Molecular and Biochemical Characterization of a Novel Xylanase from Massilia sp. RBM26 Isolated from the Feces of Rhinopithecus bieti

( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.

Evaluate VTS benefits: A case study of Zhoushan Port

Jun-Min MOU,Cui ZHOU,Yu DU,Wei-Ming TANG 국제이네비해양경제학회 2015 International Journal of e-Navigation and Maritime Vol.3 No.1

It has been well acknowledged that Vessel Traffic Services (VTS) has played a growing important role to ensure the safety of navigation in the busy ports and waterways. However, the benefits produced by VTS are usually ignored by the public and private sectors. Besides, the previous evaluations generally exist following problems: (1) It is difficult to collect the data for the parameters in the evaluation models and/or the parameters are designed illogically; (2) Those models did not take the following factors into consideration such as reducing the frequency of coastal vessel patrolling and saving human and material resources; (3) It is difficult to clearly discriminate the benefits derived from VTS and non-VTS. In this paper, a framework is presented to calculate the benefits of VTS in China. Four key indicators (safety, traffic efficiency, environmental protection and reducing supervising cost) and quantitative methods have been introduced into the framework. When calculating the benefits quantitatively, the traffic condition before the construction (expansion) of the VTS has acted as a benchmark. For a case study, the project of the expansion of VTS in Zhoushan Port, East China was evaluated with 10-year data. According to the results, the largest contribution is from the benefit of environmental protection. Via Cost-benefit analysis the benefit cost ratio (B/C) of the VTS is up to 5.248, which shows the benefits produced by VTS are considerable. The research could provide references for VTS benefits evaluation and investment optimizing.

Synthesis of pore-expanded mesoporous materials using waste quartz sand and the adsorption effects of methylene blue

Jun-Ming Hong,Bing Lin,Jie-Shan Jiang,Bor-Yann Chen,Chang-Tang Chang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Mesoporous material Q-MCM and EX-Q-MCM were synthesized using waste quartz (99.9% SiO2) sand as a silicon source via the sol–gel method. The silicon source was extracted from the waste quartz sand using the hydrothermal method. This study selected methylene blue as a target dye to investigate the dye adsorption performance onto the EX-Q-MCM. The effects on adsorption performance under different initial dye concentrations and different pH values were also studied. The results show that the maximum adsorption capacity was 96.9 mg g 1 and a high pH is favorable for adsorption. Further, equilibrium isotherms of the adsorption of methylene blue on EX-Q-MCM are well described by the Freundlich model. It reveals that the adsorption of methylene blue onto the Q-MCM is a multilayer adsorption.

Expert Perceptual Behavior under the Spatiotemporal Visual Constraints in Table Tennis

Ming-Yuan Tang,Chih-Mei Yang,Hank Jun-Ling Jwo 대한운동학회 2021 아시아 운동학 학술지 Vol.23 No.3

[OBJECTIVES] The perceptual ability to detect movement is essential for expert table tennis players. A spatiotemporal occlusion paradigm was employed to examine the critical information that facilitates athletes’ perception. [METHODS] Thirty-one expert table tennis players, 29 participants and 2 demonstrators, volunteered to participate in the study. Four types of temporal conditions and five types of spatial occlusions were displayed in experimental videos of two opponents playing a table tennis forehand stroke. Period t1-4 represented the four temporal conditions, with 250, 500, 750, and 1000 ms of action being occluded, respectively. The five types of spatial occlusion involved showing the kinematics of only the ball, paddle, arm, trunk, or head. The participants were instructed to judge the landing direction of the ball on the basis of the information in the footage. [RESULTS] The footage depicted the longest period of play. Furthermore, in separate trials, the spatial information (for the ball, torso, or head) was missing because of occlusion. The absence of such critical spatiotemporal information impaired the ability of players to make an accurate prediction. [CONCLUSION] Players obtained crucial spatiotemporal information if the timeframe of the video was relatively complete and spatial information on the opponent’s torso and head was available. For peak performance, expert table tennis players perceive and detect the optical flow of the ball’s flight and consider invariant information concerning their opponent’s torso and head.

A Bottom-up Algorithm for XML Twig Queries

Tang Zhi-xian,Feng Jun,Xu Li-ming,Shi Ya-qing 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.4

Twig query is a core operation in processing and optimizing XML structural queries. Recently, various algorithms have been proposed for finding twig patterns efficiently. Most of them are based on region labeling, pay little attention on nodes level information, and require additional caches such as stacks or lists to maintain the intermediate matching results, which cause the performance bottleneck of these algorithms. In contrast to previous work, we present a bottom-up algorithm for twig queries, which does not require additional caches and introduces idea of string searching to determine binary relationship between two nodes. Be- sides, this paper presents a node filtering mechanism–PathLevel, which can also be used in other algorithms for speeding up the query. Comprehensive experiments on several datasets demonstrate our method is an effective way for twig query.

The Clamping Position Optimization and Deformation Analysis for an Antenna Thin Wall Parts Assembly with ASA, MIGA and PSO Algorithm

Ming-hui Pan,Wen-cheng Tang,Yan Xing,Jun Ni 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.3

In order to reduce the deformation and assembly error in an antenna thin wall parts assembly process, the assembly location or clamping positions need to be optimized. In this paper, the effect of clamping positions and clamping force on the thin wall parts deformation is analyzed considering the gravity by finite element simulation under different clamping force. The clamping positions are optimized with the Adaptive Simulated Annealing (ASA), Multi-Island Genetic Algorithm (MIGA) and Particle Swarm Optimization (PSO) algorithm by Isight software to reduce the deformation. Comparing with MIGA and PSO method, the results show that the clamping position optimization based on ASA exhibits the better results and superior than the MIGA and PSO no matter whether it has the effect of gravity. Simultaneously, through establishing the contact force model and contact deformation model, the nonlinear relationship between contact force, contact deformation and contact area is qualitatively analyzed, and the node deformation is calculated by theoretical formula. Comparing with those results, it is shown that the theoretical value matches well with the simulation results. Finally, some clamping scheme principles are proposed so that it provides a basis for reducing the deformation and assembly error of thin wall parts in future.

A New Chemosensing Ensemble for Colorimetric Detection of Oxalate in Water

Tang, Li-Jun,Liu, Ming-Hui Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.11

To realize highly selective recognition of oxalate in water, a new chemosensing ensemble that behaves highly selective colorimetric recognition of oxalate in water at pH 7.4 has been developed. The ensemble was constructed by a pyrrole containing mononuclear copper complex and chromeazurol S. The ensemble shows a highly selective recognition of oxalate through an obvious color change from blue to yellow upon the addition of oxalate, whereas, other dicarboxylates such as malonate, succinate, fumarate, maleate, glutarate, adipate, phthalate, isophthalate and terephthalate do not induce any noticeable color changes. The oxalate recognition process is not significantly affected by other coexisting dicarboxylate.

KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1

( Jun Jian Hu ),( Wu Zhou Yuan ),( Ming Tang ),( Yue Qun Wang ),( Xiong Wei Fan ),( Xiao Yang Mo ),( Yong Qing Li ),( Zao Chu Ying ),( Yong Qi Wan ),( Karen Ocorr ),( Rolf Bodmer ),( Yun Deng ),( Xiu 생화학분자생물학회 2010 BMB Reports Vol.43 No.1

Experimental quantum fast hitting on hexagonal graphs

Tang, Hao,Di Franco, Carlo,Shi, Zi-Yu,He, Tian-Shen,Feng, Zhen,Gao, Jun,Sun, Ke,Li, Zhan-Ming,Jiao, Zhi-Qiang,Wang, Tian-Yu,Kim, M. S.,Jin, Xian-Min Springer Science and Business Media LLC 2018 Nature photonics Vol.12 No.12

Global prevalence of depression and anxiety in patients with hepatocellular carcinoma: Systematic review and meta-analysis

Darren Jun Hao Tan,Sabrina Xin Zi Quek,Jie Ning Yong,Adithya Suresh,Kaiser Xuan Ming Koh,Wen Hui Lim,Jingxuan Quek,Ansel Tang,Caitlyn Tan,Benjamin Nah,Eunice Tan,Taisei Keitoku,Mark D. Muthiah,Nichola 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.4

Background/Aims: Depression and anxiety are associated with poorer outcomes in patients with hepatocellular carcinoma (HCC). However, the prevalence of depression and anxiety in HCC are unclear. We aimed to establish the prevalence of depression and anxiety in patients with HCC. Methods: MEDLINE and Embase were searched and original articles reporting prevalence of anxiety or depression in patients with HCC were included. A generalized linear mixed model with Clopper-Pearson intervals was used to obtain the pooled prevalence of depression and anxiety in patients with HCC. Risk factors were analyzed via a fractional-logistic regression model. Results: Seventeen articles involving 64,247 patients with HCC were included. The pooled prevalence of depression and anxiety in patients with HCC was 24.04% (95% confidence interval [CI], 13.99–38.11%) and 22.20% (95% CI, 10.07–42.09%) respectively. Subgroup analysis determined that the prevalence of depression was lowest in studies where depression was diagnosed via clinician-administered scales (16.07%;95% CI, 4.42–44.20%) and highest in self-reported scales (30.03%; 95% CI, 17.19–47.01%). Depression in patients with HCC was lowest in the Americas (16.44%; 95% CI, 6.37–36.27%) and highest in South-East Asia (66.67%; 95% CI, 56.68–75.35%). Alcohol consumption, cirrhosis, and college education significantly increased risk of depression in patients with HCC. Conclusions: One in four patients with HCC have depression, while one in five have anxiety. Further studies are required to validate these findings, as seen from the wide CIs in certain subgroup analyses. Screening strategies for depression and anxiety should also be developed for patients with HCC.