스테로이드 의존성 천식 환자에서 스테로이드와 β2-아드레날린 수용체 mRNA의 발현양상

반준우 ( Joon Woo Bahn ),오선영 ( Sun Young Oh ),김세훈 ( Sae Hoon Kim ),장윤석 ( Yoon Seok Chang ),김우경 ( Woo Kyung Kim ),김상훈 ( Sang Hoon Kim ),김윤근 ( Yoon Keun Kim ),조상헌 ( Sang Heon Cho ),민경업 ( Kyung Up Min ),김유영 대한천식알레르기학회 2003 천식 및 알레르기 Vol.23 No.2

Background:Steroid-dependency is one of phenotypes of severe asthma. Because steroid- dependent asthmatics require long-term systemic steroids to control their symptoms, they have potentially increased risks to experience serious adverse events caused by

정신분열병 환자의 삶의 질과 방식

반건호,김종우,윤도준,유희정,신영우 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.2

연구목적 : 최근에 환자의 주관적 행복감과 삶의 질이 정신과 뿐만 아니라 일반 의학적인 치료효과 판정의 지표로 대두되고 있다. 본 연구는 정신분열병 환자에서 병식의 유무에 따른 삶의 질에 어떠한 차이가 있는지 알아보고, 그 임상적 의미와 치료전략에서의 유용성에 대해 고찰하고자 하였다. 방 법 : DSM-Ⅳ 진단 기준에 의한 55명의 정신분열병 입원환자를 대상으로 하였다. Amador등(1994)이 개발한 Scale for Unawareness of Mental Disorders(SUMD)를 이용, 병식이 있는 군(22명)과 병식이 없는 군(15)으로 나누었다. 각 군에서 Lehman(1988)이 개발한 Quality of Life Interview(QOLI)를 시행하여 병식 유무에 따른 차이를 검증하였다. 또한 Beck Depression Inventory(BDI)를 실시, QOLI의 주관적 척도 및 SUMD와의 상관관계를 분석하였다. 결 과 : 병식 유무에 따른 인구동태학적 지표 및 정신증상의 심각도에는 유의한 차이가 없었다. 객관적 삶의 질에서는 양 군간에 유의한 차이를 보이지 않았다. 주관적 삶의 질에서는, 병식이 있는 군에서 사회적 관계에 대한 만족도 영역에서 유의하게 낮은 수준을 보였다. (t=2.61, p〈.05), 또한 병식이 있는 군에서 통계적으로 유의하지는 않으나 주관적 척도의 총합과 안전에 대한 만족도 영역에서 낮은 경향을 나타냈다. BDI, SUMD, QOLI의 주관적 척도 사이에는 의미있는 부적 상관관계를 보였다. (p〈.05). 결 론 : 병식이 있는 정신분열병 환자에서 주관적으로 낮은 행복감을 보이며, 특히 대인관계에서의 만족도가 낮은 것으로 나타났다. 그 이유에 대해서는, 우울증상과의 관계가 시사되었으나 다른 요인들에 대한 향후 연구가 필요하다고 생각된다. 정신분열병 환자를 치료함에 있어 삶의 만족도에 관심을 갖고, 환자가 불만족스럽게 느끼는 부분을 중점적으로 지지해준다면 치료효과를 향상시킬 수 있을 것이다. Objectives : This study was designed to evaluated differences in the quality of life (QOL) according to insight in patients with schizophrenia. The author speculated that insight might have an effect on individual's QOL, especially subjective QOL. Method : The study group consisted of patients with schizophrenia(N=55). Subjects were divided into two group, patients with insight(N=22) and without insight(N=15), based on Scale of Unawareness of Mental Disorder(SUMD). All of the patients were administered the Quality of Life Interview(QOLI) by Lehman and compared in objective and subjective QOL between two groups. Additionally, they completed BDI and F scale on MMPI. Results : The patients with insight showed a tendency of lower scores in total subjective QOLI score and the safely issues compared to the patients without insight although not significant statistically. And they showed significantly less satisfaction with social relations. In objective QOL, there are no statistically significant difference between two groups. Conclusion : Such difference support the notion that schizophrenic patients with insight are less satisfied with their lives, especially in the aspect of interpersonal relations. It reflects the awareness of functional decline due to lifetime disability, disconnection from social relations, poor resources and supports, social stigma, and also depressive or anxiety symptoms. The authors propose that the treatment strategies for schizophrenia must include concern and support for domains of life with which patients feel themselves least satisfied, to increase effectiveness and efficacy of treatment and improve QOL.

Brain Succinic Semialdehyde Dehydrogenase; Reaction of Arginine Residues Connected with Catalytic Activities

Bahn, Jae-Hoon,Lee, Byung-Ryong,Jeon, Seong-Gyu,Jang, Joong-Sik,Kim, Chung-Kwon,Jin, Li-Hua,Park, Jin-Seu,Cho, Yong-Joon,Cho, Sung-Woo,Kwon, Oh-Shin,Choi, Soo-Young Korean Society for Biochemistry and Molecular Biol 2000 Journal of biochemistry and molecular biology Vol.33 No.4

The succinic semialdehyde dehydrogenase from bovine brain was inactivated by treatment with phenylglyoxal, a reagent that specifically modifies arginine residues. The inhibition at various phenylglyoxal concentrations shows pseudo-first-order kinetics with an apparent secondorder rate constant of 30 $M^{-1}min^{-1}$ for inactivation. Partial protection against inactivation was provided by the coenzyme $NAD^+$, but not by the substrate succinic semialdehyde. Spectrophotometric studies indicated that complete inactivation of the enzyme resulted from the binding of 2 mol phenylglyoxal per mol of enzyme. These results suggest that essential arginine residues, located at or near the coenzyme-binding site, are connected with the catalytic activity of brain succinic semialdehyde dehydrogenase.

정신분열증 입원환자에서 니코틴과 항정신병 약물의 상관관계 규명을 위한 니코틴 패취 적용 연구

반건호,유희정,윤도준,김종우,이기철 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

연구목적 : 정신분열증 환자는 흡연율이 높고, 그에 따라 항정신병 약물 투여량이 늘어나고, 약물에 의한부작용은 오히려 감소한다고 알려져 있다. 이러한 연구에서 방법론적 문제로는 실제 니코틴 용량을 측정하지 않았다는 점이다. 본 연구는 담배보다 니코틴의 주요 대사물인 코티닌을 측정하여 방법론적 오류를 최소화하고자 하였다. 방법 : 정신분열증으로 진단받은 입원환자 중 니코틴 패취군 8명, 대조군 8명을 설정하였다. 5일간 패취를 붙인 후 혈액에서 코티닌을 측정하였고, 정신증상의 정도를 알기 위하여 PANSS, 약물의 추체외로 부작용 측정을 위하여 ESRS를 실시하였다 일일 약물용량을 chlorpromazine 동량으로 환산하여 기록하였다. 결과 : 코티닌 수치와 정신증상, 추체외로부작용, 약물용량 사이에는 유의한 차이가 없었다. 결론 : 코티닌은 항정신병 약물의 작용에 영향을 미치지 못하였다. 단, 향후 연구에서는 니코틴 패취의 양을 증량하여 사용하므로써, 코티닌 용량에 따라 항정신병 약물효과에 영향을 미칠 수 있는지를 검증해야 할 것이다.

응급실에 온 의도적 자해 환자들의 정신과적 특성

한승우(Seung-Woo Han),유희정(Hee-Jeong Yoo),채수광(Su-Kwang Chae),윤도준(Doh-Joon Yoon),반건호(Geon-Ho Bahn) 대한사회정신의학회 2004 사회정신의학 Vol.9 No.1

목 적: 자해로 응급실에 내원한 환자들은 단순히 자살 행동으로 간주되는 경향이 있다. 하지만 실제로 자살하려는 행동과 의도적 자해 (intentional self-harm, 이하 IHS)는 명확히 구분되어야 할 필요가 있을 것으로 생각된다. 본 연구의 목적은 의도적 자해를 주증상으로 본원 응급실에 온 환자들의 임상적 특징에 대해 검토하였다. 환자들의 임상적 특징들을 조사하여 자해와 자살의 개념을 명확히 하는데 도움이 되고자 하는 것이다. 방 법: 1995년 1월부터 1998년 12월까지 IHS를 주증상으로 경희대병원 응급실에 내원하여 정신과 진료를 받은 환자 152명(여자 103명, 남자 49명)의 병록지를 토대로 하여 조사하였다. 남녀성비, 연령, 방문계절, 자해도구, 자해부위, 자해정도, 자해동기, 추정 되는 정신과 진단명, 정신과 과거력, 자해 과거력, 응급실 방문 후 치료형태 등으로 드러나는 IHS 특징들을 규명하였다. 결 과: 1) 성비는 2.17:1의 비율로 여자에서 더 빈번하게 나타났다. 환자 연령분포는 남녀 모두 20대가 가장 많았다. 평균 연령은 여자 31.3세, 남자 32.6세였다. 2) 의도적 자해의 가장 흔한 수단은 약물이나 화학물질이었고, 약물 가운데는 정신과에서 처방 받은 약물이 39%로 가장 높았다. 3) 두 번째로 흔한 수단은 손목 절단이었으며, self mutilation의 정도는 대부분 경도, 중등도의 손상 수준이었다. 4) IHS의 가장 흔한 동기는 대인관계나 가정 내 갈등이었다. 5) IHS의 약 54%에서 정신과 질환이 동반되는 것으로 나타났지만, 이들 중 61.2%가 정신과 치료를 받지 못 하였다. 6) IHS의 과거력이 있는 경우는 14.2%였다. 결 론: IHS는 단순 자살 행동과는 달리 다양한 의미를 내포하고 있고, 성별, 나이 등의 여러 변수에 따라 양상이 다르게 나타났다. 따라서, IHS를 주소로 내원한 환자 진료 시에는 그 개념을 세분하는 것이 필요하며, 자살과는 분화된 개념으로 환자에게 접근하는 것이 필요할 것으로 생각된다. Objectives:Patients admitted to the emergency room with self-injurious behavior has been regarded simply as suicidal act. However, it needs to be distinguished bewteen actual suicide and deliberate self harm. Thus, this study was performed to examine the clinical characteristics of patients with intentional self harm(ISH). Methods:The hospital charts of 152 patients(female:103, male:49) with self injurious behavior having evaluated by psychiatrist at the emergency room at Kyunghee University Hospital from January, 1995 to December, 1998. Clinical characteristics of intentional self harm were examined by sex, age, means, injury degree, motive, underlying psychotic disorders, and past history of ISH. Results:The female to male ratio was 2.17:1. The incidence increases markedly among third decade age group. Mean age of female patients was 31.3 and 32.6 for males. The most common method for intentional self harm was drugs or chemicals(60%). Among them, drug prescribed in the department of psychiatry was the highest(39%), and the wrist cutting was the second. Regarding the self mutilation, most of them were superficially or moderately injured. The most frequent reason for intentional self harm were interpersonal or intrafamilial conflicts. 54% of patients were supposed to be accompanied with psychiatric disorder, but 61.2% of patients had no history of psychiatric treatment. 14.2% of them also had previous history of intentional self harm. Conclusion:It is necessary to consider whether it is appropriate to classify ISH as a simple act when no comorbidities are diagnosed. And it is necessary to classify the injurious behavior into suicidal act and self mutilation. Psychiatrists should make different strategies to manage these self injurious patients whenever it is possible.

Association between genetic variations of vascular endothelial growth factor receptor 2 and atopy in the Korean population

Park, Heung-Woo,Lee, Jong-Eun,Shin, Eun-Soon,Lee, Jae-Young,Bahn, Joon-Woo,Oh, Heung-Bum,Oh, Sun-Young,Cho, Sang-Heon,Moon, Hee-Bum,Min, Kyung-Up,Elias, Jack A.,Kim, You-Young,Kim, Yoon-Keun Elsevier 2006 The journal of allergy and clinical immunology Vol.117 No.4

<P><B>Background</B></P><P>Vascular endothelial growth factor (VEGF) has been suggested to be a key mediator in the development of atopy and T<SUB>H</SUB>2 inflammation.</P><P><B>Objective</B></P><P>We sought to evaluate the effects of variations in the gene coding VEGF receptor (VEGFR) 2 on intermediate phenotypes of asthma in the Korean population.</P><P><B>Methods</B></P><P>A cohort of 2055 children and adolescents responded to a questionnaire concerning asthma symptoms and risk factors and underwent methacholine bronchial challenge and skin tests. The <I>VEGFR2</I> gene, including the promoter area, was sequenced on 24 healthy subjects to discover informative single nucleotide polymorphisms (SNPs; minor allele frequency >2%). After haplotype reconstruction, 4 tagging SNPs (IVS6+54A>G, +889G>A, +1416T>A, and IVS25-92G>A) were scored. These SNPs were also scored in 480 adult asthmatic patients to verify the above genetic association study.</P><P><B>Results</B></P><P>The prevalence of atopy was associated with a single SNP (+889G>A) of VEGFR2 with borderline significance (<I>P</I> = .048; relative risk, 1.13; 95% CI, 1.00-1.28). However, haplotype analysis showed that the atopy prevalence was strongly associated with a haplotype (AGAG) of VEGFR2 (<I>P</I> = .002; relative risk, 1.25; 95% CI, 1.09-1.42). As for airway hyperresponsiveness, neither individual SNPs nor haplotypes were found to be associated. Interestingly, the significant association was also found between atopy and the AGAG haplotype among adult asthmatic patients (<I>P</I> = .008; odds ratio, 1.66; 95% CI, 1.14-2.44).</P><P><B>Conclusions</B></P><P>The present study demonstrated that genetic variations of VEGFR2 are significantly associated with atopy in the Korean population.</P>

Comparative Transcriptome Analysis Reveals Novel Roles of the Ras and Cyclic AMP Signaling Pathways in Environmental Stress Response and Antifungal Drug Sensitivity in Cryptococcus neoformans

Maeng, Shinae,Ko, Young-Joon,Kim, Gyu-Bum,Jung, Kwang-Woo,Floyd, Anna,Heitman, Joseph,Bahn, Yong-Sun American Society for Microbiology 2010 EUKARYOTIC CELL Vol.9 No.3

<B>ABSTRACT</B><P>The cyclic AMP (cAMP) pathway plays a central role in the growth, differentiation, and virulence of pathogenic fungi, including Cryptococcus neoformans. Three upstream signaling regulators of adenylyl cyclase (Cac1), Ras, Aca1, and Gpa1, have been demonstrated to control the cAMP pathway in C. neoformans, but their functional relationship remains elusive. We performed a genome-wide transcriptome analysis with a DNA microarray using the <I>ras1</I>Δ, <I>gpa1</I>Δ, <I>cac1</I>Δ, <I>aca1</I>Δ, and <I>pka1</I>Δ <I>pka2</I>Δ mutants. The <I>aca1</I>Δ, <I>gpa1</I>Δ, <I>cac1</I>Δ, and <I>pka1</I>Δ <I>pka2</I>Δ mutants displayed similar transcriptome patterns, whereas the <I>ras1</I>Δ mutant exhibited transcriptome patterns distinct from those of the wild type and the cAMP mutants. Interestingly, a number of environmental stress response genes are modulated differentially in the <I>ras1</I>Δ and cAMP mutants. In fact, the Ras signaling pathway was found to be involved in osmotic and genotoxic stress responses and the maintenance of cell wall integrity via the Cdc24-dependent signaling pathway. Notably, the Ras and cAMP mutants exhibited hypersensitivity to a polyene drug, amphotericin B, without showing effects on ergosterol biosynthesis, which suggested a novel method of antifungal combination therapy. Among the cAMP-dependent gene products that we characterized, two small heat shock proteins, Hsp12 and Hsp122, were found to be involved in the polyene antifungal drug susceptibility of C. neoformans.</P>

Brain Succinic Semialdehyde Dehydrogenase : Reaction of Arginine Residues Connected with Catalytic Activities

Choi, Soo Young,Bahn, Jae Hoon,Park, Jinseu,Jin, Li Hua,Lee, Byung Ryong,Kim, Chung Kwon,Cho, Sung-Woo,Jeon, Seong Gyu,Cho, Yong Joon,Jang, Joong Sik,Kwon, Oh-Shin The Korea Science and Technology Center 2000 BMB Reports Vol.33 No.4

The succinic semialdehyde dehydrogenase from bovine brain was inactivated by treatment with phenylglyoxal, a reagent that specifically modifies arginine residues. The inhibition at various phenylglyoxal ocncentrations shows pseudo-first-order kinetics with an apparent second-order rate constant of 30 M-¹min-¹ for inactivation. Partial pretection against inactivation was provided by the coenzyme NAD+, but not by the substrate succinic semialdehyde. Spectrophotoetric studies indicated that complete inactivation of the enzyme resulted from the binding of 2 mol phenylglyoxal per mol of enzyme. These results suggest that essential arginine residues, located at or near the coenzyme-bindig site, are connected with the catalytic activity of brain succinic semialdehyde dehydrogenase.

Association between haplotypes of genetic polymorphisms of IL-13 and GM-CSF and atopy, airway hyperresponsiveness, and atopic asthma

( Sang Heon Kim ),( Heung Woo Park ),( Yong Eun Kwon ),( Yi Young Chung ),( Tae Bum Kim ),( Hye Kyoung Park ),( Sun Young Oh ),( Joon Woo Bahn ),( Heung Bum Oh ),( Eun Soon Shin ),( Jong Eun Lee ),( Y 대한내과학회 2004 대한내과학회 추계학술발표논문집 Vol.2004 No.1

Leukotriene C4 Synthase 유전자 Promoter 부위 유전적 다형과 아스피린 과민성 천식의 연관성

김상헌 ( Sang Heon Kim ),박흥우 ( Heung Woo Park ),손성욱 ( Seong Wook Sohn ),이상민 ( Sang Min Lee ),오선영 ( Sun Young Oh ),김선신 ( Sun Sin Kim ),박준우 ( Joon Woo Bahn ),장윤석 ( Yoon Seok Chang ),신은순 ( Eun Soon Shin ),이 대한천식알레르기학회 2006 천식 및 알레르기 Vol.26 No.2

Background: The 5-lipoxygenase, 5-lipoxygenase associated protein and leukotriene C4 (LTC4) synthase are enzymes involved in the synthesis of cysteinyl leukotrienes which are known to be important lipid mediators for the development of aspirin-intolerant asthma (AIA). Several studies have evaluated genetic association between polymorphisms of these enzymes and AIA, reporting inconsistent results. Objective: To evaluate the genetic effect of polymorphisms of promoter areas of ALOX5, ALOX5AP and LTC4S genes in the development of AIA. Method: Polymorphisms in promoter areas of ALOX5 (-1761G>A, -1708G>A), ALOX5AP (21A/18A repeat) and LTC4S (-444A>C) were genotyped in patients with AIA (n=44), aspirin-tolerant asthma (ATA, n=104), and healthy-normal controls (NC, n=107). Result: The frequency of LTC4S -444 AC or CC alleles was significantly lower in AIA patients than in ATA patients (19.0% vs. 36.5%, P=0.005, OR=0.23, 95%CI 0.08∼0.64). However, the frequency of these genotypes was not different between AIA and NC (19.0% vs. 24.8%, P=0.458). As to LOX5 -1761G>A, -1708G>A, and ALOX5AP 21A/18A repeat, the frequency of these genotypes was not different between AIA and ATA patients and between AIA and NC. Conclusion: A significant association was found between LTC4S -444A>C and the frequency of AIA. This finding suggests that LTC4 synthase may be involved in the development of AIA in the Korean population. (Korean J Asthma Allergy Clin Immunol 2006;26:115-121)