발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

의료기기 QI 활동 개선방안에 대한 연구

강훈희,주라형,김종순,김서확,허수진 한국의료QA학회 1998 한국의료질향상학회지 Vol.5 No.2

Background : Medical equipments take a very important role in diagnosis and treatment of disease in modern medicine and effective maintenance of the equipments is a necessory to provide a good healthcare to the public. After developing a new QC program for effective maintenance of medical equipments and practicing it for a year, we report the results of the new program. Methods : The maintenance data of 9 equipments in 8 categories including a CT Scanner were analyzed with regard to the parts responsible for most frequent failure and cause of the failure. After learning the most frequent failure part and cause of the failure, we developed a new QC program that emphasizes preventive maintenance of the most frequent failure part. We compared the number of failure per year and active rate of each equipment before, and after the adoption of the new QC program. Results : The average number of failure per year per equipment was 20.7 before and it decreased by 43% to 11.9 after adoption of the new QC program. The average active rate of the equipments was 92.6% before and it increased by 3.2% to 95.8% after adoption of the new program. Conclusions : The practice of the new QC program appears very useful as it decreased the failure rate and increased the active rate of the equipments.

초정밀 절삭가공에서 표면거칠기 특성 평가

강순준,김종관 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.1

In this study. experiments were conducted with an ultra-precision machine. developed in domestic. to find the characteristics and the most suitable cutting conditions of ultra-precision machining. To maximize the performance of the machine, the machine was installed in a room that is protected from vibration and is maintained constant temperature and constant humidity. Selected work pieces are an aluminum-alloyed material, which has excellent corrosion resistance and has loll deformation. The used tool is synthetic poly crystal diamond, which has excellent abrasion resistance and has low affinity, Four types of tool nose radius were used such as 0. 0.1, 0.2 and 0.4mm. machining is performed with cutting speed of 500, 800 and 1000m/min. feed rate of 0.005, 0.008, 0.01mm/rev. and cutting depth of 0.0005, 0.0025 and 0.005mm respectively which can generally be used in the field as a cutting condition. As a method of evaluation, surface roughness was measured for each cutting condition. and reciprocal characteristics are computed for each tool nose radius. cutting speed, feed rate and cutting depth. As a result. the most suitable cutting condition and characteristics of ultra-precision machining were identified which can usefully be applied in the industrial field.

고등학교 계열별 학업 성취도에 관한 연구

강현중,박종순 瑞逸大學 1999 論文集 Vol.17 No.-

근래들어 2년제 대학(전문대학)의 입학에 실업계 출신 학생들의 정원 비율이 높아지고 있다. 특별전형의 방법이 다양해지고 정원도 늘면서 전체 입학생 중 실업계 고교 출신자들의 비율이 늘고 있는 것이다. 전문대학이 중견 전문기술인의 양성을 목표로 하고 있기 때문에 이는 바람직한 방향으로 볼 수도 있으나 다른 한편으로 실업계 출신 학생들의 학업 성취도가 일반계 고교 출신 학생들보다 낮다고 생각하며 우려하는 시각이 있는 것도 사실이다. 이러한 관점에서 실제로 실업계 고교 출신 학생들과 일반계 고교 출신 학생들의 학업 성취도에 차이가 있는 지를 알아보기 위해 본교의 재학생을 대상으로 간단한 실험적 분석을 하였다.

초기치 문제를 극복한 그림자 정보의 형상복구

강창순,배종갑 동의공업대학 1999 論文集 Vol.25 No.1

In this paper, we propose the new reconstruction algorithm of shape from shading without regularization. The proposed algorithm can overcome the initial value problem by using the singular point for the estimation of the surface normal. In our approach, it is assumed that the illuminant source direction is known and the object surface is uniform Lambertian. The normal vector of the given image pixel is calculated from the illuminant direction vector, the brightness of the image, and the singular point that has the local maximum of brightness. Computing speed in our method is faster than in other ones, since normal vectors are computed directly. In addition, it gives good results for the convex surfaces in contrast to other methods. Simulation results from synthetic image are provided to demonstrate the performance of the proposed algorithm and compare it with the performance of miminimum downhill principle.

윤활유 종류에 따른 주축 열변위의 특성 평가

강순준,이갑조,김종관 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.2

High speed precision machines have been introduced to the CNC industry in order to improve productivity, shorten the appointed date of delivery and reduce the prime cost. High speed machines have more functions then general maches, and they were proved in performance. The production and sales of the high speed machines have been increased not only in domestic market but also all over the world. Accordingly, machines are faster, there are lots of problems to be solved. One of the most difficult problems is the thermal displacement on the main spindle due to generated heat while the spindle is rotated in high speed. Since the thermal displacement directly effects the quality of the machined parts, utmost efforts to minimize the thermal displacement have to be given from the beginning of designing machines. In practice, variety of methods are attempted and practiced to minimize the thermal displacement such as design of symmetrical lime, adoption of high speed bearings. application of compensation system using non-contact sensor and use of forced circulating lubrication system with oil cooler. Even if these variable methods have been practically used in the industrial field, generated heat has not been perfectly prevented. Hence, in this pager, the characteristics of thermal displacement were investigated when several kinds of oil were tested for a high speed machine with forced circulating lubrication system within the same atmosphere and under the same conditions.

SEM 측정법에 의한 초정밀 표면가공 특성연구

강순준,오상록,이갑조,김종관 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

The purpose of this paper is to look at the characteristics of surface finishing which is one of the form accuracies and to obtain the fundamental technical data from the process of machining with diamond tool through experiment and theoretical analysis. The experiments were conducted with domestic made ultra-precision machine and MCD·PCD tool, with aluminum alloyed material and brass being used for the work pieces. The goal of the size accuracy was set to 100nm. The most suitable tool nose radius and machining conditions were selected, and the variations of the surface roughness were observed using SEM method while machining the distance of up to 500km. These data were evaluated and they examined the variation of the machined surfaces while cutting up to 500km of machining distance. At the same time, the state for the wear of diamond tool nose was analyzed and carefully examined through the newest measuring device. Additionally, the characteristics of ultra-precision machining technology were studied through visual analysis.

새로운 반복이완법에 의한 선소의 정합

강창순,배종갑 동의공업대학 2000 論文集 Vol.26 No.1

The line segment matching problem in stereo vision is solved by a new iteration scheme. A match function which directly reflects the requirements of the epipolar and disparity constraints is proposed for line segment matching. The information contained in the match function can be used to determine line segment correspondences indirectly. After a match network is established according to the match function values, a new iteration algorithm is employed to tune the strengths of the match links in the match network so that the match network can converge to a stable state. No explicit compatibility coefficient need be defined for computing the support function values in the iterations, so that computation time can be saved, compared with conventional relation matching techniques. The inherent anti-symmetric characteristic of relaxation matching for the correspondence problem is also avoided naturally. The experimental results show that the proposed iteration scheme is effective and suitable for various stereo images.

HPLC-ECD를 이용한 흰쥐의 뇌 중 감마아미노부티르산 및 글루탐산의 정량

강종성,이순철 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and γ-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with ο-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C_18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na_4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.

外筒과 윗板이 回轉하는 二重圓筒內에서의 定常層流 流動解析

姜昌壽,權純範,李鍾鵬 경북대학교 공과대학 1981 工大硏究誌 Vol.10 No.-

In this paper, steady laminar flow in a tank of double cylinder with rotating outer cylinder and upper disc was analyzed numerically. For simplicity, the stream function and vorticity have been introduced into the Navier-Stokes and the continuity equations. The transformed governing equations were converted into the fimite difference equations which were solved numerically by ADI (Alternating direction implicit) and SUR (Successive under relaxation) method.