의료기기 QI 활동 개선방안에 대한 연구

강훈희,주라형,김종순,김서확,허수진 한국의료QA학회 1998 한국의료질향상학회지 Vol.5 No.2

Background : Medical equipments take a very important role in diagnosis and treatment of disease in modern medicine and effective maintenance of the equipments is a necessory to provide a good healthcare to the public. After developing a new QC program for effective maintenance of medical equipments and practicing it for a year, we report the results of the new program. Methods : The maintenance data of 9 equipments in 8 categories including a CT Scanner were analyzed with regard to the parts responsible for most frequent failure and cause of the failure. After learning the most frequent failure part and cause of the failure, we developed a new QC program that emphasizes preventive maintenance of the most frequent failure part. We compared the number of failure per year and active rate of each equipment before, and after the adoption of the new QC program. Results : The average number of failure per year per equipment was 20.7 before and it decreased by 43% to 11.9 after adoption of the new QC program. The average active rate of the equipments was 92.6% before and it increased by 3.2% to 95.8% after adoption of the new program. Conclusions : The practice of the new QC program appears very useful as it decreased the failure rate and increased the active rate of the equipments.

소방공무원의 외상유형에 따른 정서경험 및 외상증상의 차이 비교

강훈희,유성진 한양사이버대학교 종합연구원 2018 사이버교육연구 Vol.12 No.2

The purpose of this study was to verify the hypothesis that the PTSD symptoms and emotional experiences experienced by fire fighting officers show systematic differences depending on the type of trauma. Differentiated the trauma type into Personal, Impersonal, Direct and Indirect trauma and typical trauma scenarios were created, representing each type of trauma. Then presented the trauma scenarios to 121 fire fighting officers and PTSD symptoms and emotional experiences that each scenario triggered were evaluated. The results of the study are as follows. First, personal trauma provoked more PTSD symptoms(hyper-arousal) and negative emotions(anger, depression, guilt and shame) than impersonal trauma. Second, direct trauma provoked more PTSD symptoms(avoidance, hyper-arousal, intrusion, sleep disturbance, emotional numbing and dissociation) and negative emotions(anger, anxiety, depression and shame) than indirect trauma. Third, the interaction effects of factor 1 (personal/impersonal trauma) and factor 2 (direct/indirect trauma) were partially observed. Based on these findings, the theoretical and clinical implications of this study were discussed and the limitations were mentioned. 본 연구의 목적은 소방공무원이 체험하는 외상증상과 정서경험이 외상유형에 따라서 체계적인 차이를 보인다는 가설을 검증하는 것이었다. 외상유형을 대인 외상과 비대인 외상, 직접 외상과 간접 외상으로 구분하여 각각의 외상유형을 대표하는 전형적인 외상상황 시나리오를 작성하였다. 아울러 외상상황 시나리오를 121명의 소방공무원에게 제시하고, 각각의 시나리오가 촉발하는 외상증상과 정서경험을 평정하였다. 연구 결과는 다음과 같다. 첫째, 대인 외상이 비대인 외상보다 외상증상(과각성)과 부정 정서(분노감, 우울감, 죄책감, 수치심)를 더 강하게 유발하였다. 둘째, 직접 외상이 간접 외상보다 외상증상(회피, 과각성, 침습, 수면장애, 정서마비, 해리)과 부정 정서(분노감, 불안감, 우울감, 수치심)를 더 강하게 유발하였다. 셋째, 요인1(대인/비대인 외상)과 요인2(직접/간접 외상)의 상호작용효과가 부분적으로 관찰되었다. 이를 토대로, 본 연구의 이론적 및 임상적 시사점을 논의하였고 제한점을 언급하였다.

코로나19 기간에 경험한 생활 스트레스와 보상소비성향 간의 관계에서 내현적 자기애의 조절효과

강훈희,정구철 한국웰니스학회 2022 한국웰니스학회지 Vol.17 No.2

코로나19로 인해 발생한 일상생활의 변화는 다양한 스트레스를 유발하였다. 그러나, 코로나19로 인해 외부활동이 극히 제한되었으며, 스트레스로 인해 발생한 부정적 감정들을 해소하기 위해 인터넷 쇼핑 등을 활용한 보상소비행동이 나타나고 있다. 본 연구는 성인의 스트레스와 부정적 정서 상황에서의 보상소비성향 간의 관계에서 내현적 자기애의 조절효과를 분석하기 위해 수행되었다. 연구의 대상은 19세에서 39세까지의 성인 300명이었다. 자료의 분석은 분산분석과 상관관계분석 및 회귀분석을 수행하였다. 연구 결과, 여성이 남성보다 스트레스, 내현적 자기애 및 보상소비성향이 유의하게 높게 나타나, 여성을 위한 지원이 필요하였다. 그리고, 스트레스, 내현적 자기애 및 보상소비성향 간에는 유의한 정적 상관관계가 있었다. 마지막으로 스트레스와 보상소비성향 간의 관계에서 내현적 자기애는 유의한 조절효과가 있었다. 이러한 결과는 코로나19기간 중 경험한 스트레스에 대해 보상소비를 통해 해소하는 경향이 내현적 자기애가 높은 집단에서 발생하는 것을 알 수 있었다. 스트레스로 인한 부정적 감정을 해소하기 위해 부적절하게 수행하는 보상소비를 조절할 수 있도록 내현적 자기애의 조절에 대해 논의하였다. We have all experienced changes to our daily life due to the COVID-19 pandemic, which has caused different kinds of stress in different individuals. Because of the lockdowns, outside activities have been extremely limited. Reward consumption behaviors such as online shopping are emerging, which are said to relieve negative emotions caused by stress. This study was conducted to analyze the moderating effect of covert narcissism on the relationship between adult stress and the tendency to engage in compensatory consumption during negative emotional situations. The subjects of the study were 300 adults aged 19 to 39 years. Data analysis was performed using analysis of variance, correlation analysis, and regression analysis. According to the results, women showed significantly higher levels of stress, covert narcissism, and compensatory consumption propensity than men, and therefore support for women was needed. Furthermore, there was a significant positive correlation between stress, covert narcissism, and the propensity of compensatory consumption. Lastly, covert narcissism had a significant moderating effect on the relationship between stress and compensatory consumption propensity. These results show that the tendency to relieve stress experienced during the COVID-19 period through compensatory consumption is more pronounced in the group with high covert narcissism. The control of covert narcissism was discussed in order to regulate the inappropriate compensatory consumption and relieve the negative emotions caused by stress.

소음노출 후 흰쥐 내이 와우유모세포의 형태적 변형

이석기,강훈희,공미경,이광선,정종우 대한이비인후과학회 2005 대한이비인후과학회지 두경부외과학 Vol.48 No.8

Background and Objectives:Mechanism of inner ear hair cell distortion after noise exposure has been well described. The present study was designed to determine the response to the auditory system of a genetically well-defined laboratory mouse in preparation for examining the effect of noise on mice with specific genetic mutations. So it is important to recognize the relationship between noise exposure duration and hair cell morphological changes. We try to reveal the hearing loss and inner ear hair cell morphological changes after applying the noise protocol. Subjects and Method:The mice were BALB/c hybrids and aged 8 weeks. Six mice served as non-noise-exposed controls and 8 mice were exposed for 3 hours per day to white band noise with a center frequency from 0.2 kHz to 70 kHz and a sound pressure level of 120 dB. And we divided the noise exposure group into 3 subgroups(1 day, 3 day, 5 day noise exposure group). We checked the photographs of FITC phalloidin stain and scanning electron microscopy of cochlea after noise exposure. Results:The hearing level of mice decreased after noise exposure. We could see the stereocilia damage in cochlea after FITC phalloidin stain in cochlea and sterocilia loss was more severe in basal turn. In scanning electron microscopy, morphological changes of stereocilia were observed to be more severe in the cochlear basal turn than other area. Significant hair cell loss in the cochlear basal turn could be calculated using cochleocytogram. Conclusion: 120dB broad white band noise can damage the hair cell of cochlea in mice. These changes were especially severe in the cochlearbasal turn. Noise exposure duration is the other important factor in damaging cochlear hair cells. Therefore, we can guess that harmful noise level and noise exposure duration are the main risk factors that injure the inner ear hair cell.

흰쥐 내이에서 CoCl2 전 처치에 의한 소음성 난청의 예방 효과

신정은,강훈희,정종우 대한이비인후과학회 2007 대한이비인후과학회지 두경부외과학 Vol.50 No.9

Background and Objectives:1α (HIF-1α) acording to time after being exposed to noise trauma and find out the efect of HIF-1 α in the prevention of noise-induced hearing los by pre-treatment with cobalt chloride (CoCl2). Subjects and Method:BALB/c hybrid mice with 25 dB HL or les ABR were used in this study. In the study group, subjects were exposed to 120 dB SPL broad white band noise for 3 hours per day for 3 days. The changes in their hearing were documented before and after 1, 2, 3, 4, and 9 days of the first noise exposure. CoCl2 α on noise-induced hearing los. For the control, injection with distilled water was performed and hearing thresholds were measured in the same manner. Cochlea from each group was obtained in order to observe the morphological changes in the iner ear and the expression of the HIF-1α using imunohistochemial staining and imuno-fluorescein staining along with quantification of the hair cell los. Results:Mice exposed to the noise for 3 days, showed permanent threshold shift and the expression of HIF-1α was increased. When HIF-1α was induced by pre-treatment of CoCl2was observed to some degree. And hair cel survival rate was also higher when treated with CoCl2 compared to the distilled water treated group. Conclusion:When pre-treated with CoCl2, inducing HIF-1α before the noise trauma, it allowed for a less stereocilia los in the hair cells in the organ of Corti. HIF-1α may play an important role in the prevention of noise-induced hearing loss. (Korean J Otorhinolaryngol-Head Neck Surg 2007 ;50 :743-9)

기니픽의 정원창에 투여된 Bafilomycin에 의한 청력 및 와우내 전위 변화

안중호,강훈희,정종우 대한이비인후과학회 2004 대한이비인후과학회지 두경부외과학 Vol.47 No.6

흰쥐에서 소음자극 후 Hypoxia-Inducible Factor의 내이내의 발현

신정은,강훈희,정종우 대한이비인후과학회 2004 대한이비인후과학회지 두경부외과학 Vol.47 No.8

:Investigations regarding the mechanisms of noise-induced hearing los have sugested that noise exposure induces tissue ischemia, and damages the inner ear. The hypoxia-inducible factor (HIF), a helix-lop-helix PAS transcription factor, is known to prevent hypoxic cel damage in the brain in cases of ischemic attacks. Like the role it plays in the brain, there is a posibility that HIF could be found in the inner ear playing the similar role. Materials and Method:We used 23 (46 ears)threshold was measured by ABR before and after exposing mice to 120 dB SPL broad band white noise 3 hours daily for 5 consecutive days. And after 1 month, the hearing threshold was rechecked to assure the permanent threshold shift. Cochlea were collected for the histological study and the expression of HIF was observed. Results:Control mice showed hearing level of 27.5± 1.7 dB (mean± SD), which was changed to 80.5± 2.1 dB immediately after 5-day of exposure to noise, and 79.5±s noticeable. HIF was expresed in the spiral limbus, few suporting cels, stria vascularis and spiral ganglion cells of the noise exposed mouse. Conclusion:Noise, a form of acoustic trauma to the inner ear, induced damage to the outer hair cels in mice. At the same time, HIF were expresed in all of the studied animals. The posibility of HIF playing a role of protecting the inner ear from the acoustic trauma as it does in the brain should be investigated further.

저산소증을 유발한 기니픽에서 청력역치의 변화 및 외림프액내 유산염 농도의 변화

안중호,강훈희,정종우 대한이비인후과학회 2002 대한이비인후과학회지 두경부외과학 Vol.45 No.12

Background and Objectives:Authors aimed to observe the change of hearing threshold and the concentration of perilym-Materials and Method:Healthy guinea pigs were anesthetized with xylazine and ketamine and perfomed thracheotomy with ventilatory care. After shaving around vertex and mastoid area, the minimum ABR threshold was checked until the wave I was normally traced. Hypoxic condition was induced by the increase of N2 and the decreased O2 saturation was confirmed via monitor, and then the ABR threshold was rechecked. Then, the mastoid cavity was opened and round window was identified under microscopic view. Labyrinthine perilymphatic fluid was collected from a round window by Hamiltons syringe and the lactate level was calculated. Three guinea pigs were used as controls and the perilymphatic fluids were collected. Results:In the guinea pig with normal Preyers reflex, the initial hearing threshold was 29.5± 3.6 dB SPL (mean± SD) and the average ABR threshold of guinea pig with hypoxic state was 71.0± 1.4 dB SPL. The average perilymphatic lactate level was 5.39± 0.07 mg/dL in the guinea pig of normal hearing and 60.024± 1.66 mg/dL in the guinea pig of hypoxic state. Conclusion:Authors observed that hearing threshold was decreased and perilympahatic lactate level was elevated via induced hypoxia. Through these findings, authors can draw the conclusion that lactate may be one of the possible factors explaning the mechanism of the change in the hearing threshold by tissue ischemia. (Korean J Otolaryngol 2002 ;45 :1125-9)

유아ㆍ놀이중심 교육과정 실행력 강화를 위한 학습공동체에 참여하는 원장의 경험 분석

고영미,강훈희,김미란,김은영,남상미 한국유아교육학회 2020 정기학술대회 논문집 Vol.2020 No.1

Matrigel uses in cell biology and for the identification of thymosin b4, a mediator of tissue regeneration

Hynda K. Kleinman,김경순,강훈희 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.6

The thin extracellular matrix that is found basally in epithelial and endothelial cells and around smooth muscle, peripheral nerves, and fat cells is known as the basement membrane. A murine tumor matrix extract, termed Matrigel, has provided an abundant source of basement membrane proteins (laminin, collagen IV, heparan sulfate, etc.). Matrigel gels at room temperature into a structure similar to the authentic matrix. Embryonic tissue explants, stem cells, and various cell types differentiate when cultured on Matrigel. Matrigel has been used in various in vitro assays for angiogenesis, cell invasion, spheroid formation, organoid formation from a single cell, etc. In vivo Matrigel improves/promotes tumor xenograft growth and is used to measure angiogenesis, improve heart and spinal cord repair, increase tissue transplant take, etc. Endothelial cells plated on top of Matrigel form capillarylike tubules. The gene for thymosin beta 4 was induced at 4 h after plating endothelial cells on Matrigel, and when the thymosin beta 4 protein was added exogenously to the culture, tubule formation was accelerated. Thymosin beta 4, a small 43 kDa protein present in all body fluids and cells, has multiple biological activities, including reducing inflammation, apoptosis, and cytotoxicity while increasing cell migration, stem cell recruitment and differentiation, and tissue repair. Thymosin beta 4 was subsequently found to promote angiogenesis in vivo and to improve dermal and ocular healing in experimental injury models. It has regenerative activity in animal models of traumatic brain injury, stroke, multiple sclerosis, heart attack, peripheral neuropathy, liver and kidney fibrosis, and hair growth. Clinical trials have demonstrated its efficacy for both stasis and pressure ulcers and for both dry eye and a rare ocular disease. This mini review will discuss the development of Matrigel and the discovery of thymosin beta 4 as a regenerative protein that is upregulated when endothelial cells are plated on Matrigel.