"하고초(夏枯草)"의 생약학적 연구

황명석,조창희,박종희,Whang, Myung-Suk,Cho, Chang-Hee,Park, Jong-Hee 한국생약학회 2000 생약학회지 Vol.31 No.2

'Ha Go Cho (夏枯草)' is one of the Chinese crude drugs used mainly as a diuretic. With regard to the botanical origin of 'Ha Go Cho', it was reported by Su et al. that those from China were originated from the fruited spica of Prunella vulgaris L. of Labiatae. It was, however, for the herba or spica of Prunella vulgaris L. var. lilacina Nakai from Korea. According to survey of markets in Korea, most of the materials collected in the markets seemed to be originated from Prunella plant, while some seemed to be Thesium plant of Santalaceae. To clarify the botanical origin of 'Ha Go Cho', the anatomical characteristics of Prunella vulgaris L. var. lilacina Nakai and Thesium chinense Turcz. were studied. As a result, it was clarified that some 'Ha Go Cho' from Korea were the herba or spica of Prunella vulgaris var. lilacina, whereas some others were the herba of Thesium chinense.

Notes on the Korean Ascomycetes (IX)

Cho,Duck-Hyun,Lee,Jong-Il 한국자원식물학회 2002 Plant Resources Vol.5 No.2

Many ascomycetes were collected at Mt. Minjuji, Mt. Manduck, Mt. Odae, Mt. Yonsuk and Mt. Sunun from August 1999 to September 2000. They were identified. According to the reusulting, Helvella villosa (Hedw.: O. Kuntze) Dissing and Nannfedt, Dasychyphus corticalis (Pers.:Fr.) Mass., Lachnellula fuscosanguinea(Rhem) Dennis, Lachnellula subtilissima (Cke.) Dennis and Scutellina erinaceus (Schw.) Kunrze are newly to Korea. They were designed Korean common names by authors.

Unrecorded Species of Cordyceps used Oriental Medcine Resources

Cho,Duck-Hyun,Lee,Jong-Il 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Many higher fungi were collected at Mt.Sunun, Mt.Kangchon and Mt.Moak from June, 1991 to April, 2003. They were identified and surveyed with references. According to the result, Cordyceps clavata, C. cocciniocapite, C. ryougamimontanna, C. tuberculate J. moelleri and C. yakushimensis are unrecorded species to Korea. They were designed Korean common names by authors. Common names: Cordyceps clavata, C. cocciniocapite, C. ryougamimontanna, C. tuberculate f moelleri, C. yakushimensis, unrecorded species, common names.

2. Phytomitogen Induced Blast Trmsformation of Korea Native Goat

Jong_shu Kim,K. C. Lee,J.H. Lee,H.J. Cho 한국실험동물학회 1987 한국실험동물학회 학술발표대회 논문집 Vol.1987 No.-

α1,3-Gal Knock out Pigs Increases N-Glycolylneuraminic Acids

Jong-Yi Park,Mi-Ryung Park,Hong-Thuy Bui,Deug-Nam Kwon,Min-Hui Kang,Mihye Oh,Jae-Woong Han,Ssang-Goo Cho1,Chankyu Park,Hosup Shim,Hye-Min Kim,Man-Jong Kang,Jin-Ki Park,Jeong-Woong Lee,Kyung-Kwang Lee 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

In this study, we examined whether Hanganutziu-Deicher (H-D) antigens are important as an immunogenic non-a1,3-galactose (Gal) epitope in pigs with a disrupted a1,3- galactosyltransferase gene. The targeting efficiency of the AO blood genotype was achieved (2.2%) in pig fibroblast cells. A total of 1800 somatic cell nuclear transfer (SCNT) embryos were transferred to 10 recipients. One recipient developed to term and naturally delivered two piglets. The a1,3-galactosyltransferase activity in lung, liver, spleen, and testis of heterozygote a1,3-galactosyltransferase gene knockout (GalT-KO) pigs was significantly decreased, whereas brain and heart showed very low decreasing levels of a1,3- galactosyltransferase activity when compared to those of control. Enzyme-linked lectinosorbent assay showed that the heterozygote GalT-KO pig had more sialyla2,6- and sialyla2,3- linked glycan than the control. Furthermore, the heart, liver, and kidney of the heterozygote GalT-KO pig had a higher N-glycolylneuraminic acid (Neu5Gc) content than the control, whereas the lung of the heterozygote GalT-KO pig had Neu5Gc content similar to the control. Collectively, the data strongly indicated that Neu5Gc is a more critical xenoantigen to overcoming the next acute immune rejection in pig to human xenotransplantation.

消積白朮散의 癌 轉移 抑制와 T 및 B 임파구의 증식에 미치는 영향

Jung, Chel-Jong,Son, Chang-Kyu,Cho, Chong-Kwan 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Chel-Jong Jung, Chang-Kyu Son, Chong-Kwan Cho. Department of Oriental Internal Medicine, Oriental Medical Hospital, Taejon University, Taejon, Korea. Studies on the effects of sojekbaekchoolsan on immune response & metstasis of B16-Fo melanoma cells. Proceedings of International Symposium on East-West Medicine, Seoul. 117-127, 1999.-This study was carried out to investigate the effect of Sojeokbaekchoolsan on immune response and metastasis of B16-Fo melanoma cells. The result were summarized as follows:1. Metastasisi of B16-Fo melanoma cell in C57BL/6 mice were decreased in the Sojeokbaekchoolsan treated group as compared with the control group. 2 Blastic response of lymphocyte to Con A were increased in the Sojeokbaekchoolsan teated group as compared with the control group. 3. Blastic response of lymphocyte to LPS were increased in the Sojeokbaekchoolsan treated group as compared with the control group. 4 Blastic response of lymphocyte to PWM were increased in the Sojeokbaekchoolasn treated group as compared with the control groupl From the above results, It is suggested that Sojeokbaekchoolsan enhanced as well cellula-mediated and humoral immune response as sojeokbaekchoolsan supressed the metastasis of tumor cells as compared with the control group.

Antimutagenic Effects of Ginsenoside Rb$_1$, Rg$_1$ in the CHO-K1 Cells by Benzo[a]pyrene with Chromosomal Aberration Test and Comet Assay

Kim, Jong-Kyu,Kim, Soo-Jin,Rim, Kyung-Taek,Cho, Hae-Won,Kim, Hyeon-Yeong,Yang, Jeong-Sun The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.2

The usage and types of chemicals are advancing, specializing, large-scaled increasing, and new chemical exposed workers are concerning to occupational disease. The generation of reactive oxygen in the body from carcinogen, mutation and DNA damage in cancer is protected by natural antioxidants (phytochemicals) with antimutagenic effect. There were many reports of ginsenoside Rb$_1$, Rg$_1$ grievances of the genetic mutation to suppress the effect confirm the genetic toxicity test with chromosomal aberration test and the Comet (SCGE) assay confirmed the suppression effect occurring chromosomal DNA damage. We had wanted to evaluate the compatibility and sensitivity between the chromosomal aberration (CA) test and the Comet assay. We used the CA test and Comet assay to evaluate the anti-genotoxicity of ginsenoside Rb$_1$ and Rg$_1$, in CHO-K1 (Chinese hamster ovary fibroblast) cell in vitro, composed negative control (solvent), positive control (benzo[a]pyrene), test group (carcinogen+variety concentration of ginsenoside) group. The positive control was benzo[a]pyrene (50 $\mu$M), well-known carcinogen, and the negative control was the 1 % DMSO solvent. The test group was a variety concentration of ginsenoside Rb$_1$, Rg$_1$ with 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10%. In chromo-somal aberration test, we measured the number of cells with abnormally structured chromosome. In Comet assay, the Olive tail moment (OTM) and Tail length (TL) values were measured. The ratio of cell proliferation was increased 8.3% in 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10% Rb$_1$ treated groups, and increased 10.4% in 10$^{-10}$%, 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1% Rg$_1$ treated groups. In the CA test, the number of chromosomal aberration was decreased all the Rb$_1$ and Rg$_1$ treated groups. In the Comet assay, the OTM values were decreased in all the Rb$_1$ and Rg$_1$ treated groups. To evaluate the compatibility between CA and Comet assay, we compared the reducing ratio of chromosomal abnormalities with its OTM values, it was identified the antimutagenicity of ginsenoside, but it was more sensitive the CA test than the Comet assay. Ginsenoside Rb$_1$ and Rg$_1$ significantly decrease the number of cells with chromosomal aberration, and decrease the extent of DNA migration. Therefore, ginsenoside Rb$_1$, Rg$_1$ are thought as an antioxidant phytochemicals to protect mutagenicity. The in vitro Comet assay seems to be less sensitive than the in vitro chromosomal aberration test.

Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

사면구조 격자를 이용한 이동경계 기법 개발

김종호,이승오,조용식,Kim. Jong-Ho,Lee. Seung-Oh,Cho. Yong-Sik 한국방재학회 2008 한국방재학회 학술발표대회논문집 Vol.2008 No.1

All measures to cope with flooding rely on flood predictions to some extent, and the effectiveness of these measures is dependent on the quality of flood predictions. It is important to track properly the movements of the river-bankline in numerical modeling because the location of it varies continuously in the flood inundation. In this study, the wet and dry treatment is used to describe the moving river-bankline accurately (Cho, 1996). An oscillatory flow motion in a parabolic basin is used to validate the performance of the developed model based on quadtree grids. As a result of a simulation, a reasonable agreement is observed with analytical and Cho`s results.

Selection of sawdust alternative substrates for oyster mushroom bottle cultivation

Jeong-Han Kim,Jong-In Cho1,Yun-Hae Lee,Yeo-Hwang Moon 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Poplar or pine sawdust is used to main substrates for oyster mushroom bottle cultivation. However, sawdusts are containing a lot of lignin that is insoluble and non digestible component. Therefore, spent substrates which are produced by oyster mushroom harvest are difficult to use as ruminant feed. In this study, we carried out to find sawdust substitute on the oyster mushroom for ruminant feed. As results of chemical analysis of cotton seed pellet, corn stem pellet, and corncob was revealed that concorb showed low crude ash(2.4%) and lignin(13.1%) contents. Mushroom yield and biological efficiency in concorb substrates is similar to and higher than that of control. Therefore we estimated that corncob could be substituted for sawdust.