Overexpression of NDRG2 Can Inhibit Neuroblastoma Cell Proliferation through Negative Regulation by CYR61

Zhang, Zhi-Guo,Li, Gang,Feng, Da-Yun,Zhang, Jian,Zhang, Jing,Qin, Huai-Zhou,Ma, Lian-Ting,Gao, Guo-Dong,Wu, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Several recent studies have showed that the n-myc downstream regulated gene 2 (NDRG2) is a new tumor suppressor gene, and that it plays an important role in tumor suppression in several cancers or cancer cell lines. However, few studies focused on its function in neuroblastoma cells. In the present investigation, we demonstrated that NDRG2 overexpression inhibited their proliferation. Using a cDNA microarray, we found that overexpression of NDRG2 inhibited the expression of cysteine-rich protein 61 (CYR61), a proliferation related gene. From our research, CYR61 may partially hinder NDRG2-mediated inhibition of cell proliferation. Overexpression of NDRG2 resulted in accumulation of cells in the G1 phase, which was accompanied by upregulation of p21 and p27 and downregulation of CDK4 and cyclin D1. Taken together, these data indicate that NDRG2 inhibits the proliferation of neuroblastoma cells partially through suppression of CYR61. Our findings offer novel insights into the physiological roles of NDRG2 in neuroblastoma cell proliferation, and NDRG2 may prove to be effective candidate for the treatment of children with neuroblastoma.

Metastasis associated genomic aberrations in stage II rectal cancer

Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.

Performance Comparison of Analytical Models for Rotor Eccentricity: A Case Study of Active Magnetic Bearing

Zhi Cao,Yunkai Huang,Baocheng Guo,Jianning Dong,Fei Peng 한국자기학회 2020 Journal of Magnetics Vol.25 No.2

This paper applies two different analytical methods, i.e., the perturbation method and superposition method, to calculate the magnetic flux density distribution and the magnetic force of the active magnetic bearing (AMB) with the rotor eccentricity. These two methods are thoroughly analyzed, compared and validated by the finite element model (FEM). The perturbation method is theoretically complex while the superposition method is intuitive. The valid range of the superposition method is larger than the perturbation method. However, the superposition method requires longer computation time. The main contribution of this paper is assessing the effectiveness of two analytical methods for predicting the AMB performance with the rotor eccentricity and giving a comprehensive guideline for engineers to choose the proper analytical method to design AMB.

Synchronous Ectopic Pancreatoblastoma in a Child: A Case Report

Zhi-Hao Yang,Jian-Bo Gao,Song-Wei Yue,Xue-Hua Yang,Hua Guo 대한의학회 2011 Journal of Korean medical science Vol.26 No.6

Pancreatoblastoma is a rare primary pancreatic neoplasm of children that may arise in any portion of the pancreas. We report a case of a 3-yr-old boy who presented to with abdominal pain our hospital and a progressive bulge in his right abdomen. Biochemical evaluation and serum levels of tumoral markers were within reference limits. On the computed tomography, two tumors were found. One located in the head of the pancreas;however, a laparotomy revealed that the head of pancreas was compressed but normal. The other was in the left abdomen near the spleen and the tail of the pancreas. The diagnosis of two synchronous pancreatoblastoma originating from the omentum was confirmed by pathology. Therefore, a pancreatoblastoma should be considered when a large well-defined, lobulated, and heterogeneous mass is identified in the pancreas of children. In addition, an ectopic pancreatoblastoma should be considered when identified within or near the ectopic pancreatic tissue.

Differential expression of sex-related fat body proteins during the larval–pupal developmental stages of the silkworm (Bombyx mori)

Zhi-PingWu,Yan-Yan Liu,Guo-Qiang Chen,Ting-LiangWang,Jian-Zhong Tan 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1

The silkworm fat body is the site of many intermediary metabolic processes, and a source of sustenance forgrowth throughout the life cycle. Fat body proteins are responsible for storing nutrients, providing energy, andregulating hormones, and they have been identified using proteomic approaches. However, detailed differentialexpression of sex-related fat body proteins has not previously been evaluated. In the present study, we characterizedthe differential expression of sex-related fat body proteins, by using 2-dimensional gel electrophoresis(2-DE) followed bymass spectrometry identification and bioinformaticsmethods.We extracted the fat body proteinsfrom 5-day-old fifth instar larvae (L5), 10-day-old fifth instar larvae (corresponding to the end of spinning[LE]), and 0-day-old pupae (P0) of the multivoltine silkworm variety “Da Zao”. We confirmed the presence of 11important sex-specific expression proteins and 14 stage-specific expression proteins.We accurately identified 13of these specific expression proteins, including actin, calponin-like protein, 75 kDa subunit NADH, receptor foractivated protein kinase C from Bombyx mori (BmRACK), IMP (inosine monophosphate) cyclohydrolase, tropomyosin1, β-tubulin, hypothetical protein, antichymotrypsin precursor, and 30 K protein precursor.We showedthat BmRACK was differentially expressed betweenmale and female silkworms.Wediscuss the biological roles ofthe specific expression proteins during the larval–pupal developmental stages.

Invited Mini Review : Splicing and alternative splicing in rice and humans

Zhi Guo E,Lei Wang,Jian Hua Zhou 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.9

Elevated Expression of Nuclear Protein Kinase CK2α as a Poor Prognosis Indicator in Lymph Node Cancerous Metastases of Human Thyroid Cancers

Guo, Miao,Liu, Chao,Qi, Feng-Jie,Zhang, Xiu-Mei,Ren, Li-Li,Liu, Yi-Meng,Meng, Zhi-Chao,Zhu, Zhi-Tu,Xiao, Jian-Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Aim: To investigate the expression of protein kinase $CK2{\alpha}$ ($CK2{\alpha}$) in human thyroid disease and its relationship with thyroid cancer metastasis. Materials and Methods: Using immunohistochemistry we measured the expression of $CK2{\alpha}$ in 76 benign and malignant human thyroid cancer tissues, including 10 pairs of papillary carcinoma tissues with or without lymph node cancerous metastasis and similarly 10 pairs of lymph nodes. Results: The expression of $CK2{\alpha}$ was found to be higher in thyroid carcinoma cases (papillary carcinoma, follicular carcinoma, anaplastic carcinoma and medullary carcinoma) than in ones such as chronic lymphocytic thyroiditis, nodular goiter and adenoma. These findings were also confirmed by RT-PCR and Western blotting. More strikingly, elevated expression of $CK2{\alpha}$ in thyroid papillary carcinoma tissues was not only significantly associated with lymph node cancerous metastasis and clinical stage of thyroid cancers; but also correlated with epithelial-mesenchymal transition (EMT) and high tenascin C (TNC) expression. In addition, EMT and high TNC expression in thyroid carcinoma tissues was significantly associated with lymph node cancerous metastasis. Conclusions: Elevated expression of nuclear $CK2{\alpha}$ is a poor prognosis indicator in lymph node cancerous metastasis of human thyroid cancers.

Effects of Wushu-Sanda Player`s Different Exercise Intensity on the Fatigue Substance, Oxidative Stress and Antioxidant Enzyme

( Guo Jian He ),( Zhi Hong Bao ),( Tian Yu Liu ),( Shuai Li ),( Jeong Ryong Chae ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Purpose: The purpose of this study was designed to examine the activation of Fatigue Substance, Oxidative Stress and Antioxidant Enzyme in Wushu Sanda players according to the different exercise intensity. Methods & Materials: Subjects of this four-week-long Wushu exercise program were divided into 2 groups: high intensity group (n=8), and low intensity group (n=8). Subjects participated in this program once a day (high intensity group:60mins, low intensity group:90mins) and 5 times per week (Monday to Friday). Every subjects’ fatigue substance (LAC, LDH), oxidative stress (MDA) and antioxidant enzyme (GPx, SOD, CAT) were measured before the program, immediately after exercise, after 30mins recovery and after 60mins recovery. All these parameters were measured again four-week latter. Statistics: ANOVA, paired t-test and repeated ANOVA were carried out to analyze the changes before and after the exercises, changes in terms of fatigue substance, oxidative stress and antioxidant enzyme, respectively. Statistical significance is established at the .05 level (p<.05). Result: 1. Change of LAC and LDH Pre and Post exercises, analysis of the LAC have statistically significant changes in recovery 30 and 60minutes than immediately after within each group (p<.05). But according to the time of measurement there are no statistically significant difference (p>.05). And, after four weeks, LAC in both groups are lower than the concentration of before exercise. Especially, the low intensity exercise group are lower than the high intensity exercise group. But all variables are no statistically significant differences(p<.05). Pre and Post exercises, analysis of the LDH was statistically significant changes in immediately after and recovery 60minutes within each group(p<.05). And according to the time of measurement were only significantly difference in the rest (p<.05). What’s more, the high intensity exercise group are lower than the low intensity exercise group (p>.05). 2. Change of MDA MDA in low intensity group was significantly higher after exercise and 30mins recovery than before exercise (p<.05). In high intensity group, no significant difference was found. 3. Change of GPx, SOD, CAT After the four weeks exercise, GPx in high intensity group shows significant differences after 30mins and 60mins recovery compared with the value of pre-exercise (p<.05). No significant difference was found in low intensity group. Compared to the value of SOD pre-exercise, the SOD of post-exercise shows significant differences after 30mins and 60mins recovery in high intensity group ( p<.05) while only immediately after exercise in low intensity group (p<.01 ). In CAT, a significant difference was found in high intensity group after the four weeks exercise (p<.05). No significant difference was found in low intensity group. Conclusion: After four weeks exercise, some differences were found in fatigue substance(LAC, LDH), oxidative stress(MDA) and antioxidant enzyme(GPx, SOD, CAT) in both groups: LDH was found to be increased in high intensity group after the four-week exercise; MDA was increased in low intensity group after exercise and 30mins recovery than before exercise; compared with the value of pre-exercise, GPx and SOD in high intensity group shows significant differences after 30mins and 60mins recovery; while in low intensity group, the SOD difference only occurred immediately after exercise; In CAT, a significant difference was found in high intensity group but no significant difference was found in low intensity group.

Dynamic behavior analysis of push-the-bit rotary steerable bottom hole assembly

Zhi-chuan Guan,Heng Wang,Yu-cai Shi,Wei-qing Chen,Guo-shan Zhao,Jian-yun Wang,Guang-qiang Cao 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.4

Rotary steerable drilling technology is currently an advanced technology in the field of drilling engineering. A good understanding of dynamic behavior of rotary steerable system will benefit the drilling progress. Aimed at push-the-bit type of rotary steerable tool, finite element method was employed to model the rotary steerable bottom hole assembly (RSBHA) and establish the motion equation. Contact interaction between drill string and wellbore wall was analyzed and taken as the constraint condition in solving RSBHA motion equation. Force applied by steering pads was as the external force acting at the pads node. Then RSBHA motion state and bit lateral force were obtained by using Newmark method to solve the motion equation. A case was given and effects of influencing factors were analyzed. The results show that stick-slip phenomenon occurs under 60 r/min rotary speed condition and disappears when rotary speed rises to 90 r/min. Fully developed backward whirl comes into being at the near-bit stabilizer when rotary speed increases to 120 r/min. High weight on bit intensifies bit torsional vibration while larger pads’ steering force decreases the bit torsional vibration. Upper stabilizer plays a part in weakening RSBHA backward whirl under high rotary speed condition but reduces the inclination force. Time average of bit lateral force is mainly determined by pads’ steering force. Based on the results, corresponding suggestions were proposed to make the RSBHA be better used in the field applications.

Circular RNA cFAM210A, degradable by HBx, inhibits HCC tumorigenesis by suppressing YBX1 transactivation

Yu Jian,Li Wen,Hou Guo-jun,Sun Da-peng,Yang Yuan,Yuan Sheng-xian,Dai Zhi-hui,Yin Hao-zan,Sun Shu-han,Huang Gang,Zhou Wei-ping,Yang Fu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Hepatitis B protein x (HBx) has been reported to promote tumorigenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), but the mechanism awaits further investigation. In this study, we found that cFAM210A (a circular RNA derived from the third exon of transcript NM_001098801 of the FAM210A gene; CircBase ID: hsa_circ_0003979) can be silenced by HBx. cFAM210A expression was downregulated and negatively correlated with tumorigenesis in patients with HBV-related HCC. Furthermore, cFAM210A reduced the proliferation, stemness, and tumorigenicity of HCC cells. Mechanistically, HBx increased the N6-methyladenosine (m6A) level of cFAM210A by promoting the expression of RBM15 (an m6A methyltransferase), thus inducing the degradation of cFAM210A via the YTHDF2-HRSP12-RNase P/MRP pathway. cFAM210A bound to YBX1 and inhibited its phosphorylation, suppressing its transactivation function toward MET. These findings suggest the important role of circular RNAs in HBx-induced hepatocarcinogenesis and identify cFAM210A a potential target in the prevention and treatment of HBV-related HCC.