Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

뉴노멀시대, 위기청소년의 사회적 안전망 강화를 위한 이멘토링(e-mentoring)의 역할과 과제

김지혜 남서울대학교 출판국 2022 남서울대학교 논문집 Vol.28 No.-

Youth mentoring programs are widely used at home and abroad for the purpose of stabilizing adolescents' emotions, improving academic ability, and developing career capabilities. This study attempted to provide basic data for revitalizing e-mentoring for crisis youth by examining the need to expand the e-mentoring area and the role of e-mentoring operating institutions as a way to strengthen the social safety net for crisis youth. As a result of the study, first, the most discussed advantage of e-mentoring is that it is less constrained by time and space. Second, by overcoming these space-time constraints, e-mentoring can connect with people living in underprivileged areas and people in isolation, including those in other cultures. Third, you can reduce preconceptions according to the environment such as appearance and social class. Based on these research results, suggestions were made for stable settlement and activation of e-mentoring in Korea. Key Words : Youth, Mentoring, E-mentoring

Anthony Browne 그림책의 상호 텍스트성에 관한 연구

지은주,이혜은 부산유아교육학회 2003 유아교육논총 Vol.12 No.-

현대의 그림책에서는 여러 다양한 기법들이 사용되는데, 상호텍스트성도 이 중의 하나이다. 본 연구에서는 상호텍스트성을 문학적 또는 문학외적인 텍스트와 이미지의 변형 및 재구조화로 정의 내리고, Anthony Browne의 그림책을 이러한 상호텍스트적인 관점에서 분석하였다. 분석결과는 다음과 같다. 첫째, Anthony Browne의 그림책 속에는 전래동화와 신화가 숨어 있으며, 둘째, Anthony Browne의 그림책은 문학외적인 장르 즉 유명한 회화 작품들과 영화 장면들과도 상호텍스트성을 보이며, 넷째, Anthony Browne의 그림책들 간에도 인물, 배경, 스타일면에서 많은 상호텍스트성을 보인다. 이러한 상호텍스트성은 그림책을 훨씬 더 다양하고, 복합적이고, 재미있게하며, 아동으로 하여금 문학을 더욱더 확장하고 심화하게끔 한다. A variety of techniques are used in modern picturebooks, and one of these is intertextuality. We defined intertextuality as literary or extraliterary variation and restructure of text and image. We analysed Anthony Browne’s picturebooks in terms of intertextuality. Our results are followings: First, fairy tale and myth are linked with intertextuality in his books. Secondly, intrtextuality appears with extraliterary, well-known pictures and film-scenes. In the third, intertextuality appears on characters, settings, and style. Intertextuality makes children read picturebooks with very various, complex, and interesting mind, Then, intertextuality also helps children extend and deepen their understanding and imagination of the literature.

부산의 산업별 생산비용과 역내수요로 본 산업발전방향

지해명 동의대학교 경제경영전략연구소 2005 經濟經營硏究 Vol.3 No.1

A major problem behind the stagnation of Pusan economy is that the proper amount of private capital does not flow into Pusan area. To select appropriate industries for the region, this research adopts the methology of production cost and intra-regional demand for producers. Regional technical and trade coefficients are used for estimating production cost. The regional technical and trade coefficients are estimated through the product-mix approach and the entropy maximization model, respectively. The intra-regional demand is derived based on the trade coefficients, regional supply and regional demand. Following the methodology, promising industries for the region are selected. Using the multiplier analysis of MRIO model, the effect of the industries on the regional economy is evaluated. This research concludes that developing precision industry may be an alternative to promote economic growth for Pusan.

조세경쟁이 지역경제의 성장과 분배에 미치는 효과분석

지해명 한국산학경영학회 2003 산학경영연구 Vol.16 No.-

현재 지방자치단체가 지방세를 탄력적으로 운용할 수 있도록 재정적 인센티브를 부여하자는 안이 대두되고 있다. 그렇지만 지방세의 변화가 지역경제의 순환구조를 왜곡할 수 있으며, 또한 지방자치단체간 조세경쟁 촉발 가능성이 있다는 이유로 그 제도의 실행이 순조롭지 않은 상황이다. CGE 모형의 의태분석 결과를 보면 지역에서 지방세 인하경쟁이 나타나면 공공부문의 비중이 작은 지역에서는 지역경제가 성장하는 한편 공공부문의 비중이 큰 지역은 경제침체가 초래된다. 따라서 지역간 경제력 격차가 확대되며, 학력별 소득분배를 악화시키게 된다. 조세인상 경쟁이 나타나면 지역간 경제력 격차의 축소 및 학력별 소득분배의 개선 가능성은 존재하지만 전반적인 경제침체가 초래된다. 이러한 분석에 근거하면 지자체의 입장도 설득력이 있으므로 정부는 이러한 폐해가 나타날 수 있다는 가능성을 수용해야 할 것이다. 따라서 국세권 일부의 지방이양 등 정책조합을 통하여 상충관계를 극복할 수 있는 방안을 마련해야 할 것인바. 간접세는 직접세 위주의 지방세보다 생산 부문에 유의한 효과를 주므로 지방자치단체가 경제성장에 탄력적으로 대응할 수 있는 수단이기 때문이다. The Korean government issues the policy that regional government mat change the regional tax rates for economic growth. On the ground that change in regional tax rate incurs the disturbance in regional economy and entails tax competition, regional government does not accept the proposal. The CGE simulation result shows that tax competition reveals the trade-off relation between efficiency, balanced national development, and equity. Thereby, the stance of regional government can be justified by the results. Therefore central government has to grope for some reconcile means that efficiency and equity can be harmonized. The proposal can be justified by the analyses.

한국의 사회복지 전문성 연구와 사회복지계 대응의 역사에 관한 고찰 : 전문직에 대한 주요 관점을 중심으로

김지혜 남서울대학교 2011 남서울대학교 논문집 Vol.17 No.2

This study was conducted to present the proper views to solve the issues, currently being debated related to the expertise, by reviewing the history of the social welfare system’s solution to study the expertise of Korean social welfare and establish the expertise in a structure-functional view - a main view on professional jobs -. a conflict theory - based view, and a historical comparison view. As a result, in the quickening period of Social Welfare Practice, there was in demand for the practice but non-experts were mass-produced failed to institutionally establish the expertise. In the developing period of Social Welfare Practice since then, it left much to be desired in the acknowledgement on the expertise by the government and institutions, in spite of researches and on-site-efforts. Lastly, in the diffusing period of Social Welfare Practice, a conflict theory-based view and a historical comparison view emerged together. Based on these findings, this study presented the direction in a solution to conduct researches on the expertise of social welfare and to establish the expertise of the social welfare system.

데커시놀추출물의 경구투여후 흰쥐에 있어서의 약물속도론적 연구

김지혜,최송암,김동출 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

The objective of this study is to investigate the pharmacokinetic parameters of decursinol following oral administration in Sprague-Dawley rats. The plasm concentration of decursinol was determined by LC/MS with APCL positive mode. The m/z value of decursinol was observed at 247. Folowing oral administration of decursinol extract, the apparent clearance was 5.3±2.7 ml/hr/rat, the absorption half life was 2.5±0.41 hr, the elimination half life was 3.05±1.57 hr and the apparent volume of distribution was 21±12 ml/rat. The LC/MS method was successfully applied to the phar-macokinetic study of decursinol.

가족자원봉사활동에 참여하는 부모와 자녀의 가족건강성에 영향을 미치는 요인 : 부산지역 건강가정지원센터를 중심으로

박지영, 공미혜 신라대학교 부산학연구센터 2017 부산연구 Vol.15 No.1

이 연구의 목적은 부산 지역 건강가정지원센터에서 가족봉사활동에 참여하는 부모와 자녀를 대상으로 부모와 자녀 간 가족건강성의 차이를 비교하고 가족건강성에 영향을 미치는 변인을 분석하여 가족건강성 연구와 가족자원 봉사 활동의 발전에 기여하고자 하였다. 2016년 8월 부터 10월까지 부산지역 9개 건강가정지원센터에서 가족자원봉사활동에 참여하고 있는 부모와 자녀를 한쌍으로 자기보고식 설문지를 배포하고 회수하여 210부(105쌍)가 최종분석에 사용되었다. 수집된 자료는 SPSS 20.0 프로그램을 이용하여 빈도분석과 차이검증, 중다회귀분석을 실시하였다. 이 연구에서 나타난 결과를 요약하면 다음과 같다. 첫째, 가족자원봉사활동에 참여하는 부모와 자녀의 가족건강성점수는 모두 평균이상으로 나타나 자신의 가족을 건강하게 지각하고 있는 것을 알 수 있다. 둘째, 부모의 경우 학력수준이 높을수록, 월 봉사시간이 많을수록, 자녀의 경우 경제수준이 높을수록 가족건강성이 높았고, 부모와 자녀 모두 봉사활동 만족도가 높을수록 가족건강성이 높은것으로 해석된다. 셋째, 가족건강성 하위영역을 분류하여 분석한 결과, 봉사시간은 부모의 가족건강성을 비롯한 가족 문제해결능력, 가족 가치체계공유에 유의미한 영향을 미치는 변인으로 나타났지만 자녀의 경우 영향력이 미미했다. 그러나 경제수준은 자녀의 가족유대감, 가족 의사소통, 가족 가치체계공유정도에 유의미한 영향을 미치는 변인으로 나타났다.

N세대의 패션상품 쇼핑동기 및 디스플레이 태도

조지현,민경혜,류덕환 啓明大學校 生活科學硏究所 2002 科學論集 Vol.28 No.-

This study intends to provide a beneficial foundation which can help understanding of how a consumer group can be classified according to the shopping motivation of net generation. And what differences to the store display were shown differences to the store display were shown differently among them and ultimately, some concrete data which can be useful in establishing efficient store display strategies for consumer. The subject of this study were 298 female college students living in Daegu. Model sampling was done by inconvenient sampling. Modified survey based on references and former studies was used, using SPSS packages. The results were as follows. 1. Three kind of factors in the shopping motivations for fashion goods were determined for analysis of consumer groups and by which it was revealed as to be significant to classify them into the following three subdivisions : information pursuit, social & amusement pursuit, and financial & practical pursuit group. 2. N-generation consumer groups were interested in the store display and used it frequently, while they were effected by the satisfied with it positively. 3. It has shown the positive relation between interest and use, interest and effect and use and effect of store display, respectively. 4. N-generation consumer groups were conscious of the necessity and effect of store display highly. And lack of originality o store display and too much goods in display area were indicated to have to be improved.