성악골 절제술후 발생한 중앙부및 구개 결손부의 재건

김훈,임재석,최성원,김호겸,권종진,최미숙,김성문 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.1

There are various defects caused by trauma or resection of maignant tumor in the orofacial region, which can be reconstructed with various regional and pararegional flaps. Among these defects, it is very difficult to reconstruct palatal and midfacial defects after maxillectomy and patients have problems in speaking and swallowing of food. Therefore it is very important for surgeons to reconstruct these defects functionally and esthetically and to return the patients to the normal social activity. These defects are usually obturated with prosthodontic appliances to assist the phonation and swallowing. But nowadays surgical reconstruction by various flaps was considered and performed for better rehabilitation. For this purpose the forehead flap, the nasolabial flap, the tongue flap, the sternocleidomastoideous flap, the temporal flap, the latissimus dorsi flap, the scapular flap etc. are used. We reconstructed small-sized plalatal defects with tongue flap, medium-sized palatal and maxillary defects after maxillectomy with temporal myofascial flap and large midfacial defects including eyeball exenteration with latissimus dorsi myocutaneous flaps. Here we are to report 5 cases of these flaps used for the reconstruction of palatal and midfacial defects and consider the versatility, reliability and limitation in use of these flaps.

선천성 고혈압 흰쥐와 정상혈압 흰쥐의 혈관 평활근 세포의 성장 특성

정재군,정현주,김인겸,김중영 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 선천성 고혈압 흰쥐(SHR)와 정상 혈압 흰쥐(WKY)의 혈관 평활근 세포를 배양하여, 그 성장 특성을 조사하였다. 대상 및 방법 : SHR 및 WKY를 pentobarbital(50㎎/㎏)로 마취하여 적출한 흉부대동맥으로부터 혈관평활근세포를 얻었으며 이 세포를 이차배양하여 일정한 시간간격으로 trypsin을 처리하여 세포를 떼어낸후 혈구계(hemocytometer)로 세포수를 헤아렸다. 결과 : SHR과 WKY에서 얻은 혈관평활근세포에서 형태학적 차이는 없었고, SHR이 WKY보다 밀생상태에 더 빨리 도달하며 밀생상태에 도달했을때 SHR이 WKY보다 세포밀도가 더 높게 나타났다. 결론: SHR이 WKY보다 증식속도와 증식밀도가 더 높게 나타나는 것으로 보아 SHR에 성장 억제 조절 기전의 장애가 있음을 시사한다. To study the generation and processes of hypertension, characteristics of vascular smooth muscle cell were observed in cultured aortic smooth mescle cell from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). The degree of cell proliferation increased with escalating concentrations of fetal bovine serum(FBS) in cells of both SHR and WKY control rats. These results have shown that 10% FBS, which was the usual concentration in culture medium, was better concentration of medium in cell proliferation than 5% FBS, 10% calf serum, 10% horse serum, or 1% bovine serum albumin. There were no detectable differences in the morphology of cells obtained from SHR and WKY. Comparative studies have shown that cultured vascular smooth muscle cells from SHR proliferated to a higher cell number, grew to a greater density, and have greater specific growth rate than those of the WKY. These results provide possibility of a disturbances on inhibitory conrol to vascular smooth muscle cell growth in SHR.

감술제조에 관한 연구

안용근,편재영,김승겸,신철승 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

홍시를 살균처리하지 않은 것, 열처리 살균한 것, 염산처리 살균한 것과 염산처리 살균한 곶감 12%에 설탕 15% 용액을 17℃에서 36일간 발효시켰다. 무처리구와 염산처리구는 감에 부착되어 있던 유산균과 초산균으로 오염되어 산패되었다. 그래서 곶감의 산도는 4.4, 열처리한 것은 산도는 5.0, 산처리한 것은5.8, 무처리함 것은 13.5ml(0.1N NaOH/10ml sample)를 나타냈다. 에탄올 함량은 열처리구는 11.2%, 곶감은 10.0%, 산처리구는 9.4%무처리구는 6.1%였다. 가장 기호성과 안정성이 높은 것은 열처리하여 28일간 발효시킨 것이었다. 열처리구로 36일간 발효시킨 술은 총당 4.65%, 환원당 3.65%, 단백질, 0.03㎎/ml, 아미노산 0.17㎍/ml, pH 3.4, 펙틴 13.02㎍/ml, 균체수 5.75 logCFU/ml를 나타냈다. 곶감주도 기호성은 높았으나 발효속도가 낮았다. The possibility of wine making from soft and dried persimmon and the effects of treatment for persimmon on the quality of wine have been investigated. Soft persimmon was pretreated by two methods, of heat and hydrochloric acid treatment. Fermentation mash was composed of 12% persimmon and 15% of sucrose, and was fermented at 17℃ for 36 days. The fermentation using persimmons which were untreated and treated by acid was not successful because of contamination caused by lactic acid and acetic acid bacteria. Acidity of the fermented broth produced from boiled, dried, acid treated and untreated persimmon was 4.4, 5.0, 5.8 and 13.5ml(0.1N NaOH/10ml broth), respectively. Ethanol concentration of the fermented broth produced from boiled, dried, acid treated and untreated persimmon was 11.2, 10.0, 9.4 and 6.1%v/v, respectively. The wine fermented for 28day's fermentation using boiled persimmon as substrate had the best quality and stability. After 36 day's fermentation using boiled persimmon, following composition was obtained: 4.65% of total sugar, 3.65% of reducing sugar, 0.03㎎/ml of protein, 0.17 ㎍/ml of amino acid and 13.02 ㎍/m1 of pectin. The pH and yeast cell of the boiled persimmon broth were 3.4 and 5.75 logCFU/ml. Fermented wine using dried persimmon had also good quality but the fermentation rate was slow.

Ipomoea batatas Attenuates Amyloid β Peptide-Induced Neurotoxicity in ICR Mice

Jae Kyeom Kim,Soo Jung Choi,Hong Yon Cho,Young Jun Kim,Seung-Taik Lim,김창주,Eun Ki Kim,Hye Kyung Kim,Sabrina Peterson,신동훈 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.3

In this study, the protective effects of 17 Korean native plants against amyloid β peptide (Aβ)-induced oxidative stress were screened using the 2′,7′-dichlorofluorescin diacetate assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Ipomoea batatas exerted the highest protective effects against oxidative stress and was selected for further investigation. To confirm the protective activity of this extract, the I. batatas extract was fed to ICR mice that had been injected with Aβ to induce neuronal deficits. In these experiments, the extract of I. batatas significantly reversed Aβ-induced neurotoxicity as assessed by the passive avoidance test, a behavioral experiment. Moreover, I. batatas administration reduced the level of lipid peroxidation and increased catalase activities in biochemical studies using the brain tissue of mice. These results indicate that I. batatas might be beneficial against Alzheimer's disease, especially by limiting oxidative stress in the brain.

Effects of Methoxsalen from <i>Poncirus trifoliata</i> on Acetylcholinesterase and Trimethyltin-Induced Learning and Memory Impairment

KIM, Jae Kyeom,CHOI, Soo Jung,BAE, Hyeri,KIM, Cho Rong,CHO, Hong-Yon,KIM, Young Jun,LIM, Seung Taik,KIM, Chang-Ju,KIM, Hye Kyung,PETERSON, Sabrina,SHIN, Dong Hoon Japan Society for Bioscience, Biotechnology, and A 2011 Bioscience, Biotechnology, and Biochemistry Vol.75 No.10

<P>Previously, we identified methoxsalen (8-methoxy-2′,3′,6,7-furocoumarin) as the bioactive compound probably responsible for acetylcholinesterase (AchE) inhibition achieved by feeding crude extract of <I>Poncirus trifoliate.</I> To confirm the activity of methoxsalen, Institute of Cancer Research (ICR) mice were fed a control or a methoxsalen-supplemented diet for 4 weeks, and then learning and memory enhancing effects with respect to trimethyltin (TMT)-induced neurotoxicity were evaluated. The brain tissues of ICR mice were dissected after completion of the behavioral tests for biochemical analysis. Methoxsalen effectively reversed TMT-induced memory impairment on both Y-maze and passive avoidance tests. Brain AchE activity was inhibited by the oral consumption of all concentrations of methoxsalen. Moreover, the level of oxidative stress was significantly ameliorated in the groups on methodsalen containing diets. This is the first <I>in vivo</I> study conducted with methoxsalen in the field of AD research, and it indicates that further investigation of methoxsalen is warranted.</P>

Inhibitory Effect of <i>Poncirus trifoliate</i> on Acetylcholinesterase and Attenuating Activity against Trimethyltin-Induced Learning and Memory Impairment

KIM, Jae Kyeom,BAE, Heyri,KIM, Mi-Jeong,CHOI, Soo Jung,CHO, Hong Yon,HWANG, Han-Joon,KIM, Young Jun,LIM, Seung Taik,KIM, Eun Ki,KIM, Hye Kyung,KIM, Bok Yong,SHIN, Dong-Hoon Japan Society for Bioscience, Biotechnology, and A 2009 Bioscience, biotechnology, and biochemistry Vol.73 No.5

<P>Various native Korean plants were screened to find an effective acetylcholinesterase (AChE) inhibitor for the treatment of Alzheimer’s disease (AD). Among these plants, the ethanol extract of <I>Poncirus trifoliate</I> was selected for isolating the AChE inhibitor because it exhibited the highest inhibitory activity (47.31%). To separate the active compound from <I>Poncirus trifoliate</I>, solvent partition, open column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were utilized. The putative chemical structure of the AChE inhibitor was identified as <I>methoxsalen</I> by successive analysis with electron ionization mass spectrometry (EI-MS) and <SUP>13</SUP>C/<SUP>1</SUP>H-nuclear magnetic resonance (NMR). To confirm the attenuating effect of the <I>Poncirus trifoliate</I> extract against trimethyltin (TMT)-induced neurotoxicity, <I>in vivo</I> behavior tests were carried out. Our findings suggest that the <I>Poncirus trifoliate</I> extract significantly reversed TMT-induced learning and memory impairment. These results demonstrate that the <I>Poncirus trifoliate</I> extract could possess a wide range of beneficial activities for neurodegenerative disorders, notably AD.</P>

Characterization of Nutritional Composition, Antioxidative Capacity, and Sensory Attributes of <i>Seomae</i> Mugwort, a Native Korean Variety of <i>Artemisia argyi</i> H. Lév. & Vaniot

Kim, Jae Kyeom,Shin, Eui-Cheol,Lim, Ho-Jeong,Choi, Soo Jung,Kim, Cho Rong,Suh, Soo Hwan,Kim, Chang-Ju,Park, Gwi Gun,Park, Cheung-Seog,Kim, Hye Kyung,Choi, Jong Hun,Song, Sang-Wook,Shin, Dong-Hoon Hindawi Publishing Corporation 2015 Journal of analytical methods in chemistry Vol.2015 No.-

<P>Few studies have investigated <I>Seomae</I> mugwort (a Korean native mugwort variety of <I>Artemisia argyi</I> H. Lév. & Vaniot), exclusively cultivated in the southern Korean peninsula, and the possibility of its use as a food resource. In the present study, we compared the nutritional and chemical properties as well as sensory attributes of <I>Seomae</I> mugwort and the commonly consumed species <I>Artemisia princeps</I> Pamp. In comparison with <I>A. princeps, Seomae</I> mugwort had higher contents of polyunsaturated fatty acids, total phenolic compounds, vitamin C, and essential amino acids. In addition, <I>Seomae</I> mugwort had better radical scavenging activity and more diverse volatile compounds than <I>A. princeps</I> as well as favorable sensory attributes when consumed as tea. Given that scant information is available regarding the <I>Seomae</I> mugwort and its biological, chemical, and sensory characteristics, the results herein may provide important characterization data for further industrial and research applications of this mugwort variety.</P>

Responsive Surface Methodology Optimizes Extraction Conditions of Industrial by-products, <i>Camellia japonica</i> Seed Cake

Kim, Jae Kyeom,Lim, Ho-Jeong,Kim, Mi-So,Choi, Soo Jung,Kim, Mi-Jeong,Kim, Cho Rong,Shin, Dong-Hoon,Shin, Eui-Cheol Medknow PublicationsMedia Pvt Ltd 2016 Pharmacognosy magazine Vol.12 No.47

<P><B>Background:</B></P><P>The central nervous system is easily damaged by oxidative stress due to high oxygen consumption and poor defensive capacity. Hence, multiple studies have demonstrated that inhibiting oxidative stress-induced damage, through an antioxidant-rich diet, might be a reasonable approach to prevent neurodegenerative disease.</P><P><B>Objective:</B></P><P>In the present study, response surface methodology was utilized to optimize the extraction for neuro-protective constituents of <I>Camellia japonica</I> byproducts.</P><P><B>Materials and Methods:</B></P><P>Rat pheochromocytoma cells were used to evaluate protective potential of <I>Camellia japonica</I> byproducts.</P><P><B>Results:</B></P><P>Optimum conditions were 33.84 min, 75.24%, and 75.82°C for time, ethanol concentration and temperature. Further, we demonstrated that major organic acid contents were significantly impacted by the extraction conditions, which may explain varying magnitude of protective potential between fractions.</P><P><B>Conclusions:</B></P><P>Given the paucity of information in regards to defatted <I>C. japonica</I> seed cake and their health promoting potential, our results herein provide interesting preliminary data for utilization of this byproduct from oil processing in both academic and industrial applications.</P><P><B>SUMMARY</B></P><P><P>Neuro-protective potential of <I>C. japonica</I> seed cake on cell viability was affected by extraction conditions</P><P>Extraction conditions effectively influenced on active constituents of <I>C. japonica</I> seed cake</P><P>Biological activity of <I>C. japonica</I> seed cake was optimized by the responsive surface methodology.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> GC-MS: Gas chromatography-mass spectrometer, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, PC12 cells: Pheochromocytoma, RSM: Response surface methodology.</P>

Protective Effects of Kaempferol (3,4′,5,7-tetrahydroxyflavone) against Amyloid Beta Peptide (Aβ)-Induced Neurotoxicity in ICR Mice

KIM, Jae Kyeom,CHOI, Soo Jung,CHO, Hong Yon,HWANG, Han-Joon,KIM, Young Jun,LIM, Seung Taik,KIM, Chang-Ju,KIM, Hye Kyung,PETERSON, Sabrina,SHIN, Dong-Hoon Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, biotechnology, and biochemistry Vol.74 No.2

<P>To determine the effects of kaempferol, rat pheochromocytoma cells (PC12) and Institute of Cancer Research (ICR) mice were utilized as neuronal models. Using <I>in vitro</I> assays, kaempferol was shown to have protective effects against oxidative stress-induced cytotoxicity in PC12 cells. Administration of kaempferol also significantly reversed amyloid beta peptide (Aβ)-induced impaired performance in a Y-maze test. Taken altogether, the results reported here suggest that further investigation is warranted of the influence of kaempferol on pathways related to Alzheimer’s disease.</P>

[PB-0089] Voltage-dependent anion channel (VDAC) functions in the GA biosynthesis pathway in monocot plant and affects seed germination

Tae Kyeom Kim(Tae Kyeom Kim),Jae Yoon Kim(Jae Yoon Kim) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-