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Ca($OH_2$)-$H_2 O$-$CO_2$계의 기액반응으로부터 비정질 탄산칼슘의 합성 및 결정화
임재석,김가연,임굉,Im, Jae-Seok,Kim, Ga-Yeon,Im, Goeng 배재대학교 공학연구소 2004 공학논문집 Vol.5 No.1
수산화칼슘현탁액과 탄산가스를 출발물질로 15~$50^{\circ}C$의 온도에서 기액반응으로 비정질 탄산칼슘($CaCO_3$.$nH_2 O$)의 생성과정을 전기저도도의 연속측정법, X-선회절법 및 투과전자현미경법을 이용하여 조사한 결과, 반응초기생성물은 비정질 탄산칼슘으로 반응현탁액의 전기전도도는 비정질 탄산칼슘의 생성 중 크게 강하하고 있으며, 이것은 수산화칼슘의 입자표면이 비정질 탄산칼슘미립자로 뒤덮여 용해를 방해받는 것과 비정질 탄산칼슘이 용액 속에서 불안정하여 즉시 용해한 다음 석출하여 칼사이트로 전이되어 미세한 침강성 탄산칼슘이 나란히 결합한 연쇄형 칼사이트가 생성된다. 비정질 탄산칼슘이 연쇄형 칼사이트로 변화하는 동안 현탁액의 전기전도도는 급격히 회복되고 이 과정에서 고농도 수산화칼슘현탁액의 외관점도가 상승한다. 이것은 연쇄형 칼사이트의 뒤얽힘에 의한 것이며, 다시 전기전도도의 1회 회복단계 이후에는 미반응 수산화칼슘에 의하여 비정질 탄산칼슘이 생성이 소멸되어 칼사이트의 성장반응이 이루어지고 pH가 9.5이하에서 연쇄형 칼사이트는 결합부분이 먼저 용해하여 결정질 탄산칼슘으로 분리생성된다. 비정질 탄산칼슘의 생성 및 합성온도의 영역은 전기전도도법에서 $15^{\circ}C$일 때 1차 강하단계(a-단계)에서 가장 적합하다. The synthesis and crystallization of amorphous calcium carbonate($CaCO_3$.$nH_2 O$) obtained from gas-liquid reaction between aqueous solution of calcium hydroxide and carbon dioxide at 15~$50^{\circ}C$ are investigated by electrical conductometry, XRD and TEM. The results are as follows: The initial reaction products prior to the formation of precipitated calcium carbonate is amorphous calcium carbonate. The electrical conductivity values in the slurry are decreased during the formation of amorphous calcium carbonate which covers particle surface of calcium hydroxide and retard the dissolution of calcium hydroxide into the solution. that amorphous calcium carbonate is unstable in the aqueous solution and crystallizes finally to calcite by the through-solution reaction. While amorphous calcium carbonate crystallizes into chain-like calcite, the conductivity values are recovered rapidly and the apparent viscosity of slurry containing higher concentration of calcium hydroxide increase. At below pH 9.5, chain-like calcite separates into individual particles to form precipitated calcium carbonate. The formation and synthetic temperature range of amorphous calcium carbonate is most suitable a primary decreasing step(a-step) at $15^{\circ}C$ in the electrical conductometry.
백서 두개골 결손부의 골재생에 fibrin glue가 미치는 영향에 관한 실험적 연구
임재석,장현석,윤정주,이의석,박은용,서제덕,Rim, Jae-Suk,Jang, Hyon-Seok,Eune, Jung-Ju,Lee, Eui-Seok,Park, Eun-Yong,Suh, Je-Duck 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.5
Fibrin glue is composed of fibrinogen and thrombin and used in various regions for multiple use. Basic principle is that thrombin converts fibrinogen to fibrin in the presence of $Ca^{2+}$. The structure of fibrin is loose at the beginning, but after about 5 minutes a tight structure is formed under the influence of factor VIII which changes fibrin monomer into fibrin polymer. Fibrin glue is used for tissue adhesive, suture, local hemostasis, wound healing, closure of subdural space. Fibrin adhesive has been used in oral and maxillofacial surgery for hemostasis after tooth extraction in patients with coagulation disorders, skin graft fixation, reattachment of periodontal flaps, in combination with autogenous bone chips to fill the bony cavities following cyst removal, and for securing the hydroxyapatite granules for maxillary alveolar ridge augmentation. This study was designed for researching influence of fibrin glue during healing phase after making artificial bone defect.
HA/TCP 골이식재상에 이식된 지방유래 줄기세포의 골모세포로의 분화 및 골형성에 대한 연구
임재석,권종진,장현석,이의석,정유민,이태형,박정균,Rim, Jae-Suk,Gwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Seok,Jeong, You-Min,Lee, Tai-Hyung,Park, Jeong-Kyun 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.2
Aim of the study: An alternative source of adult stem cells that could be obtained in large quantities, under local anesthesia, with minimal discomfort would be advantageous. Adipose tissue could be processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). This study was performed to confirm the availability of ATSCs in bone tissue engineering. Materials amp; Methods: In this study, adipose tissue-derived mesenchymal stem cell was extracted from the liposuctioned abdominal fat of 24-old human and cultivated, and the stem cell surface markers of CD 105 and SCF-R were confirmed by immunofluorescent staining. The proliferation of bone marrow mesenchymal stem cell and ATSCs were compared, and evaluated the osteogenic differentiation of ATSCs in a specific osteogenic induction medium. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific BMP-2, ALP, Cbfa-1, Osteopontin and osteocalcin were confirmed by RT-PCR. With differentiation of ATSCs, calcium concentration was assayed, and osteocalcin was evaluated by ELISA (Enzyme-linked immunosorbant assay). The bone formation by 5-week implantation of HA/TCP block loaded with bone marrow mesenchymal stem cells and ATSCs in the subcutaneous pocket of nude mouse was evaluated by histologic analysis. Results: ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. ATSCs could be easily identified through fluorescence microscopy, and bone formation in vivo was confirmed by using ATSC-loaded HA/TCP scaffold. Conclusions: The present results show that ATSCs have an ability to differentiate into osteoblasts and formed bone in vitro and in vivo. So ATSCs may be an ideal source for further experiments on stem cell biology and bone tissue engineering.
CaO-$C_2 H_5 OH$-$CO_2$계의 기.액반응에 의한 비정질 탄산칼슘의 합성 및 결정구조
임재석,임굉,Im, Jae-Seok,Im, Goeng 배재대학교 공학연구소 2004 공학논문집 Vol.6 No.1
CaO의 첨가량에 따라 $CO_2$가스를 불어넣고 반응시간 변화에 따라 용매로 $C_2 H_5 OH$을 사용하여 에틸렌글리콜을 첨가한 CaO-$C_2 H_5 OH$-$CO_2$계의 기.액반응으로부터 비정질 $CaCO_3$의 합성과 결정구조를 전기전도도, X-선회절 및 주사전자현미경으로 조사하였다. 이 반응에서 900ml의 $C_2 H_5 OH$에 에틸렌글리콜 100ml를 첨가하고 CaO의 양을 10~40g으로 하여 $CO_2$가스를 1$\ell$/min의 유속으로 흡입시켜 얻은 합성분말의 겔형 물질을 신속히 여과, 감압하의 $60^{\circ}C$에서 건조하여 1${\mu}m$이하의 구형 vaterite상과 무정형인 비정질 $CaCO_3$을 얻었다. 그리고 비정질 $CaCO_3$의 일부는 중간생성물로서 연쇄형 calcite로 변화하는 것을 알 수 있었고 침강성 $CaCO_3$의 생성보다 먼저 초기 반응생성물은 비정질 $CaCO_3$이었고 이 경우 생성역역은 pH 7-9의 범위로 상당한 영향을 준다. 또한 비정질 $CaCO_3$은 용액 속에서 불안정하여 용해반응으로 인해서 결국 calcite로 결정화한다. 특히 비정질 $CaCO_3$은 CaO-$C_2 H_5 OH$-$CO_2$계의 반응에 의해서 침강되어 생성되거나 또는 gel상으로 된다. The synthesis and crystal structure of amorphous calcium carbonate obtained from gas-liquid reaction of CaO-$C_2 H_5 OH$-$CO_2$ system according to change of added amount of calcium oxide by blowing $CO_2$ gas and reaction time using ethanol and ethylene glycol were investigated by electric conductivity, X-ray diffraction, and scanning electron microscope. The powdery or gelatinous phases were prepared by passing $CO_2$ gas at a flow rate of 1$\ell$/min into the suspensions containing 10~40g of CaO in mixing solutions 900ml of $C_2 H_5 OH$- and 100ml of ethylene glycol. By rapid filtration and drying the both phases at $60^{\circ}C$ under reduced pressure, the phases converted to the spherical vaterite and amorphous phase. The stable phase of amorphous calcium carbonate(ACC) was formed in the region pH 7-9 but the formation regions of amorphous phase were remarkably affected by pH in the mother liquor. It seems that a part of ACC changed into chain calcite as an intermediate products. The initial reactants prior to the formation of precipitated calcium carbonate is ACC. And ACC is unstable in the aqueous solution and crystallizes finally to calcite by the through-solution reaction. Especially ACC was produced or gelatinous phase which precipitated from the reaction of CaO-$C_2 H_5 OH$-$CO_2$ system.
임재석,김선현 부천대학 2001 論文集 Vol.22 No.-
본 논문은 빠르게 성장하고 있는 조직형태의 하나인 프랜차이즈 시스템에 대한 분석이다. 기업의 확장 수단으로 직영점이 아니고 가맹점으로 확장방법을 선택하느냐 하는 요인들에 대해서 분석한다. 프랜차이즈를 이용하는 이유를 설명하는 전통적인 견해는 단순히 사업 확장을 위한 자본조달에 어려움이 있는 기업이 자본을 조달하기 위한 방법으로 이용한다는 것이 있으나 비교적 최근에는 위험을 회피하기 위한 방법으로 프랜차이즈를 활용한다는 견해, 대리 문제를 해결하기 위한 수단이라는 주장, 최적자본구조를 구성하기 위하여 이용한다는 견해, 그리고 시장 영향력을 증대시키기 위한 방법으로 프랜차이즈를 활용한다는 주장이 있다. 자원할당이론은 기업의 재무적 필요성에 의해 성립되는 것에 비해 대리이론은 다분히 조직 관리적 측면이 강조된 설명이라는 특징이 있으며, 거래비용이론과 탐색비용이론은 프랜차이즈 형성 근거를 설명하면서도 조직 관리적 측면의 시사점을 일부분 가지고 있음을 알 수 있다. This paper is about the analysis of the franchise system, an organization that is growing fast. I analyze the factors such as whether to choose the method of expanding into a member store rather than the one of direct management, as a way of expanding an enterprise. The traditional view of explaining the reason for using a franchise lies in the fact that enterprises having difficulty in raising money for simply expanding the business is using it as a way to raise its capital. On relatively recent days risk-avoiding franchises are utilized to avoid the risk accompanying the expansion or the estabilishment of enterprises. There are an assertion that a franchise is a means of solving the problem of agency, a view that it's used to organize the optimal capital structure, and an assertion that it's utilized as a way of increasing the impact on the market. Agency theory is, characteristically, mostly an explanation that stresses the aspect of organizational management, while the material allotment theory is formed by the financial need of an enterprise. We can see that the transaction expense theory and the exploration expense theory have the part of current events in the aspect of organizational management, while they explain the reason for the formation of franchises.
양악 수술후 상악전진에 의한 상순 및 비부의 연조직 변화에 대한 두부계측방사선학적 연구
임재석,최철민 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.3
In case of dentofacial deformity patient, Lefort I osteotomy and BSSRO are popular methods to correct the deformity. The purpose of this orthognathic surgery is to achieve esthetic facial appearance and proper function. Various articles have been reported on soft tissue change after orthognathic surgery in terms of esthetics. Especially a change of soft tissue in upper lip and nose is important to gain facial esthetic profile. To obtain accurate prediction of postsurgical esthetics, a proper understanding of relationship between maxillary surgical movement and soft tissue change. In other words, it is very helpful in treatment planning and progonsis of orthognathic surgery to predict soft tissue changes related to hard tissue chamge. A retrospective study of selected patients was undertaken to estimate and analyze the changes of soft tissue in upper lip and nose following surgical correction with Lefort I osteotomy and BSSRO. Preoperative, postoperative, and retention period data derived from lateral cephalometric analysis.