Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

소도시 학생의 의료비지출에 관한 조사연구 -공주군내 초등학교 아동을 중심으로-

황보연, 이유복 공주교육대학교 초등연구원 1974 公州敎大論叢 Vol.11 No.-

In order to study Medical care expenditures spent by elementary school students of Gong Ju-gun on sickness, surveys conducted on the elected 4 schools consisting of 625 students during the 9 month period from March 1 to December 20, 1973. The following results have been obtained. 1. The average number of pers persons ons per household were 6.24. 2. OF all the households of students surveyed, 47.2 per cent were for agricultual] occupations and 20.5 per cent were clerical workers. 3. Illiterates constituted 13.4 per cent, primary school graduates 36.6 per cent, high? school graduates 18.5 per cent, and colleg graduates 13.9 per cent. 4.The average monthly income per household was 34.000 won. (47,000 won out of A area and 21;000 won out of B area) 5. The monthly average Number of absences from school due to sickness were 423.9’ case. April. June. October were the Highest months of the sickness ratio which total's 3,815 case, and which means 1,008 times of absence of each sick student. 6. The sickness of most students who needed medical care was cold, tiredness, and sto-- mach ace, etc. 7. The average monthly amount of medical care spent were 1,930 won for a households expendkures and 302 won per person. (373. 4 won out of A area and 236.8 won out of B area)

영어 쓰기능력 함양을 위한 교수-학습 방법 제안 : 자기주도적 학습활동을 통하여

황보근,전병락 대구대학교 인문과학 예술문화연구소 2003 人文科學硏究 Vol.25 No.-

The ultimate object in English education is to cultivate the communicative competence. Spoken English has been put great emphasis on, while the writing skill of English has been mostly neglected. The purpose of this study is to help middle students develop and improve their English writing skill through self-directed learning activities by students themselves, presenting them various effective teaching and learning techniques. The analytical results of the hypotheses indicated the following conclusions: First, in improving writing skill in English, continuous writing activities by the self-directed learning method are more effective than the traditional one. Second, the experimental method has a positive transitional effect on improvement of the students' general English proficiency because it has beneficial effects upon other skills as well. Lastly, the continuous writing activities by the self-directed learning method made affirmative changes in the students' attitude towards and increased their interest in English more than by the traditional method.

공주지역사회 체육의 진흥방안

황보연 공주교육대학교 초등연구원 1974 公州敎大論叢 Vol.11 No.-

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韓國企業倫理에 關한 硏究

皇甫太 단국대학교 경영대학원 1981 經院論叢(壇國大學校) Vol.1 No.-

주의력 결핍·과잉행동장애(ADHD)아동의 행동변화에 미치는 놀이치료의 효과

황혜자,김보경 東亞大學校 2004 東亞論叢 Vol.41 No.-

This study was performed to find out the possibilities the play therapy to change Attention Deficit and Hyper actmy Disorder(ADHD) child's behavior. This study demonstrates the value of phenomenological approach in a qualitative case study of behavior modifications applied to one ADHD child for one yeor of three months The researcher checked the charye of child's behavior in play therapy room in each session and interview디 with his mom after each session. On the dasis of ADHD child of DSM-IV(Diasnostic and Statistical Mamal), the problematic behavior of the chila in playtherapy room was examined. Besides, the child's mom was tested K-CBCL(korean children Behavior Check list) and KPI-C(Korean Personality Inventory for Children) for the child's probcemalic behavior in dairy life. The result of this research demonstrated positive effect of the play tharapy on the on the behavior modifications of ADHD In addition, after play therapy, the child's problematic behaviors such as attention deficit and hyper ․ activity were decreased.

연폭로 근로자들에서 빈혈증 관련 인자에 관한 연구

황보영,김용배,김화성,이성수,안규동,이병국 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Objectives : This study intended to obtain the interrelationship of lead exposure indices and anemia related factors for the better health management of lead exposed workers. Methods : 502 male workers in storage battery industries as lead exposed group and 106 male office workers as contorl group were selected for this study. The examined variables wer age, work duration, smoking and alcohol crinking habit, blood zinc protoporphyin concentraion (ZPP), blood lead concentration (PbB), urinary δ-aminoevulinic acid (ALAU), hemoglobin (Hb), hematocrit (Hct), serum calcium (S-Ca), serum iron (S-Fe), total iron binding capacity (TIBC), and percentage trasferrin saturation (PTS). Results : The means of ZPP, PbB ALAU in lead exposed proup were 58.4±35.7 ㎍/㎗, 27.6±10.9 ㎍/㎗, and 1.61±2.00 mg/ℓ, respectively. These three values in lead exposed group were significantly higher than control group (P<.05). The means of Hb, Hct, S-Ca, S-Fe, TIBC, PTS in lead exposed group were 15.3±1.1 g/㎗, 47.2±4.5 %, 9.4±0.4 ㎍/㎗, 124.0±67.5 ㎍/㎗, 317.1±68.5 ㎍/㎗, and 38.7±15.2 %, respectively. Hb, Hct, S-Ca, S-Fe, and TIBC in lead exposed group were significantly lower than contrl group (P<.05). PTS was no significant difference in lead exposed group and control group (P> .05). Hct, S-Ca, S-Fe, PTS were significant positive correlation with Hb and ZPP, corrected PbB, AlAU were significant negaitive correlation with Hb in lead exposed group (P< .05). On multiple regression using ZPP as a dependent variable and PbB, S-Ca and S-Fe as independent variables after adjustment for possible confounder such as age, smoking and drinking habit, Pbb, S-Ca, S-Fe and age contributed to ZPP. Conclusions : As the result of this study, it was suggested that inclusion of S-Ca, S-Fe, PTS measurement as a anemia screening test for lead workers was highly recommended for the effective prevention and early diagnosis of anemia.

공정한 키 위탁 시스템에 대한 연구

황보성,이임영 순천향대학교 부설 산업기술연구소 1999 순천향 산업기술연구소논문집 Vol.5 No.1

It is important to manage private keys on large public networks. A Key recovery system can satisfy user key loss or law-enforced access of government. Generally, key recovery system, there are many problems at fairness between user and government. So that, this paper propose new fair key recovery system that can protect of user privacy and user can not pass by key recovery system based on Failsafe and Blind decoding scheme

기능적 언어중재 프로그램이 언어장애 아동의 언어능력에 미치는 영향

황보명 대불대학교 2003 論文集 Vol.9 No.1

This study was aimed atclarifying the effects of a Functional Language Intervention in children with language disoders. Five subjects were selected and two speech-language pathologists engaged in the functional language intervention. In the functional language intervention, activities consisted of group time, palying riddles time, following directions time, drawing time, cooking time, word-chain game time, role-playing time, searching for objects based on directions, and recreation game time. To compare pre-treatment with post-treatment in the children with language disorders, repeated-measures ANOVA was used. The results were as follows; First, the Functional Language Intervention was effective in improving the number of communicative intentions of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions. Second, the Functional Language Intervention was effective in improving the number of semantic relations of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of semantic relations. Third, the Functional Language Intervention was effective in improving the overall language abilities of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the overall language abilities.

The Anglo-Saxon Chronicle의 어순연구 : Parker 및 Peterborough본을 중심으로

황보근 대구대학교 인문과학연구소 1988 人文科學硏究 Vol.6 No.-

The main purpose of this paper is to describe synchronic word-order types of the Parker and the Peterborough manuscripts of the Anglo-Saxon Chronicle and to explain diachronic word-order changes. The manuscripts were divided, according not to the syntactic characteristics of the sentences analyzed but to the compilers, into four sections: (Ⅰ) 878∼891, (Ⅱ) 925∼955, 971∼1001, (Ⅲ)1122∼1131 and (Ⅳ) 1132∼1154. The sentences in each entry year were analyzed and counted on the basis of three clause types: main, subordinate and relative. The number of clause types in the sections of the ASC is as follows: Ⅰ Ⅱ Ⅲ Ⅳ Main Clauses 113 65 329 249 Subordinate Clauses 13 7 109 81 Relative Clauses 28 7 66 28 Description of the word-order types led to the following conclusions: 1) The dominant word-order types in the main clause are SVX, SXV and X°VS. The frequency of SVX constantly increases from Section Ⅱ through Section Ⅳ. This type tends to have the topical or the semi-topical subject and to be used as the new information sentence conjoined with the preceding X°VS type. The SXV has the topical nominal or pronominal subject, and the pre-verbal X is mostly pronominal. The high frequency of X°VS in Section Ⅰ is due to its use as "the new event order" though it has the topical subject. The decrease in both SXV and X°VS is relatively constant. 2) In the subordinate and relative clauses SXV, SVX are the two dominant orders in Section Ⅰ. But from Section Ⅱ though Section Ⅳ the decrease in SXV is constant, and is coincident with the decrease in the main clause. The reason is to be explained by the hyphothesis that the order of elements in dependent clauses will be changed by analogy to the order of elements in independent clauses.