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이온화방사선에 의한 느타리버섯(Rleurotus ostreatus)의 ITS2 염기서열 변화
이영근,장화형,한갑진,고동규,이기성 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4
rDNA의 ITS2(internal transcribed spacer 2)부위에 대한 이온화방사선(gamma-ray)의 영향을 알아보기 위해서 감마선을 조사한 느타리버섯(Pleurotus ostreatus)의 5종의 변이중에서 ITS2의 염기서열을 결정하였다. ITS2의 염기서열은 1 kGy 조사군(PO-5, PO-6, PO-15와 PO-16)에서 변화되지 않았으나, 2kGy 조사군(PO-14)에서는 1개의 염기가 치환되었다. RAPD(ramdomly amplified polymorphic DNA) 양상에 의한 유전유사도의 변화가 다양하였고 섬유소성 고체검정 배지에서도 다양한 생장특성을 나타내었다. 본 연구결과는 ITS염기서열이 이온화 방사선에 의해 선량의존적으로 변화될 수 있음을 시사해 주었다.
Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석
이영근,장화형,장유신,허재호,형석원,정혜영 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.3
본 연구에서는 감마선으로 유도된 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자들의 발현을 연구하기 위하여, B. lentimorbus WJ5의 방사선 유도 돌연변이체에서 발현되는 유전자를 DNA microarray로 동시에 탐색하였다. DNA microarray는 B. lentimorbus WJ5 genome을 무작위로 절단하여 2,000단편으로 구성하였으며, 감마선 (^(60)Co)으로 유도된 7돌연변이체의 발현을 정량적으로 관찰하였다. 클러스터 분석결과 발현된 408 유전자 중 27개가 감마선 유도 돌연변이체 모두에서 유의하게 발현이 증가되었다. 특히, 복구(mutL, mutM)에너지 대사 (acsA, sdhB, p하, yhjB, citB), protease(npr), 산화자극에 대한 환원 (HMM)관련 유전자들이 동시에 증가되었다. 이는 감마선 유도 돌연변이체들에서 자발적인 직/간접 복구 관련 유전자의 발현 증가는 방사선 노출 직후 보이는 stress response와는 다른 현상임을 나타내는 것으로 생각된다. To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA microarray. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation (^(60)Co). From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.
느타리버섯(Pleurotus ostreatus) 재배균상에 오염되는 Rhizoctonia solani 유사균의 특성
이영근,장화형,김진규,김재성,이기성 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4
느타리버섯(Pleurotus ostreatus) 재배균상은 흔히 토양, 공기 중 곰팡이에 의해 오염되어 자실체 생산량이 감소되곤 한다. 느타리버섯 재배균상에서 3종류(Rs-1, RS-2와 RS-3)의 오염균주를 분리하여 internal transcribed spacer(ITS)의 염기서열을 분석하였다. 이들의 ITS염기서열은 식물 병원성 사상균의 일종인 Rhizoctonia solani와 매우 높은 상동성을 보였다. 이들 오염균주들의 영양배지와 lignin 배지에서 균사체 생장율은 느타리버섯보다 각각 수배 및 수십배나 높았다. 세포외분비효소의 활성도는 오염균주들의 경우 느타리버섯과 비교하여 매우 낮은 활성도를 보였다. 느타리버섯과의 혼합배양 결과 오염균주들은 느타리버섯의 균사체 생장 및 자실체 형성을 심하게 저해하였다. The contamination of air- and soil-borne microbes could occur and result in the reduction of fruiting body formation during cultivation of Pleurotus ostreatus. Three contaminants(RS-1, RS-2 and RS-3) were isolated and showed higher homology to the plant pathogenic fungus, Rhizoctonia solani by internal transcribed spacer(ITS) analysis. The rates of their mycelial growth on the lignin specific medium and complete medium were several times higher than those of P. ostreatus. The activities of the extracellular enzymes of three contaminants were lower than those of P. ostreatus. During oyster mushroom cultivation(P. ostreatus) together with these contaminants, the mycelial growth and fruiting body formation of P. ostreatus was seriously repressed.
Comparison of Soft Tissue Changes between Adolescents and Adults in Class II Malocclusion Treatment
장나영,조진형,이유미,강경화,Chang, Na-Young,Cho, Jin-Hyoung,Lee, You-Mee,Kang, Kyung-Hwa Korean Academy of Orofacial Pain and Oral Medicine 2009 Journal of Oral Medicine and Pain Vol.34 No.3
The purpose of this study was to evaluate the soft tissue changes of class II adolescents and adults in respect to extraction or nonextraction. The study included 68 patients from Wonkwang Dental Hospital were categorized to adolescent extraction group, adolescent nonextraction group, adult extraction group, adult nonextraction group. Cephalometric tracing of each patient was done to compare pretreatment and posttreatment of each group, to compare the changes between groups. And among the variables that showed significancy, correlation analysis and simple linear regression were done. The results were as follows. 1. In both adolescents and adults after extraction treatment, nasolabial angle significantly increased and in both subjects after non extraction treatment, nasolabial angle significantly decreased. 2. In extraction subjects, there were positive correlation between the amount of treatment changes of vertical-U1 and E line-upper lip, the changes of vertical-L1 and E line-lower lip, the changes of vertical-L1 and vertical-Li. 3. In extraction subjects, simple regression equations of E line-upper lip, E line-lower lip, Li were calculated by regression analysis. According to the results above, it could be considered that the effect of the extraction or nonextraction treatment was greater than the effect of growth.
Hwa-Hyoung Chang,Young-Keun Lee,Jae-Sung Kim,Ki-Sung Lee,Kyu Seong Cho 한국미생물학회 2003 The journal of microbiology Vol.41 No.1
The mutational spectra in the manganese (II) peroxidase gene (mnp) of the Pleurotus ostreatus mutants induced by gamma radiation (Co60) give evidence to prove the effect of gamma radiation on the gene. mnp of each mutant was cloned, sequenced and analyzed. Among the 1941 base pairs of the sequenced region of the mnp genes of 4 mutants (PO-5, -6, -15 and -16), nine mutational hotspots on which the same base was mutated simultaneously were found, additionally 6 mutations were also found at different positions in the mnp gene. These mutation-spectra were predominantly A:TG:C transitions (50.1%). By the analysis of putative amino acid sequences, PO-5 and PO-16 mutants have 3 and 1 mutated residues, respectively. Since the mutational spectra reported herein are specific to the mnp gene, we propose that the mutational hotspots for the gamma radiation could be in the gene(s) within cells.
Chang, Hwa-Hyoung,Lee, Young-Keun,Kim, Jae-Sung,Lee, Ki-Sung,Cho, Kyu-Seong The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.1
The mutational spectra in the manganese (II) peroxidase gene (mnp) of the Pleurotus ostreatus mutants induced by gamma radiation (Co$\^$60/) give evidence to prove the effect of gamma radiation on the gene. mnp of each mutant was cloned, sequenced and analyzed. Among the 1941 base pairs of the sequenced region of the mnP genes of 4 mutants (PO-5,-6,-15 and -16), nine mutational hotspots on which the same base was mutated simultaneously were found, additionally 6 mutations were also found at different positions in the mnp gene. These mutation-spectra were predominantly A:T\longrightarrowG:C transitions (50.1%). By the analysis of putative amino acid sequences, PO-5 and PO-16 mutants have 3 and 1 mutated residues, respectively. Since the mutational spectra reported herein are specific to the mnp gene, we propose that the mutational hotspots for the gamma radiation could be in the gene(5) within cells.
Difructose Dianhydrides(DFA IV)의 랫드를 이용한 단회 및 4주간 반복 경구투여 독성시험
이창우(Chang-Woo Lee),이명렬(Myong-Lyoll Lee),김환묵(Hwan-Mook Kim),윤원기(Won-Kee Yoon),김승환(Seung-Hwan Kim),손화영(Hwa-Young Son),김형진(Hyoung-Chin Kim) 한국독성학회 2004 Toxicological Research Vol.20 No.3
This study was to investigate single and repeated-dose toxicities of DFA IV, a new<br/> candidate of nutraceutical which has preventive effect on anemia and osteoporosis. In single-dose<br/> oral toxicity study, the test article were administered once by gavage to rats at dose level of 0, 2,000<br/> and 5,000 mg/kg. No dead animal, abnormal sign and abnormal necropsy finding was found in control<br/> and treated groups. Thus the approximate lethal dose of DFA IV was considered to be higher<br/> than 5,000 mg/kg in rats. In four week repeated dose oral toxicity study, the test article was administered<br/> once daily by gavage to rats at dose levels of 0, 500, 1,000 and 2,000 mg/kg. No abnormality<br/> was observed in mortality, clinical findings, body weight changes, food and water consumptions,<br/> opthalmoscopic findings, hematological findings, necropsy findings, organ weights and histopathological<br/> findings. In urinalysis, specific gravity was increased in 2,000 mg/kg groups of male rats. In serum<br/> biochemical analysis, creatine phosphokinase was increased in all treatment groups of male rats.<br/> These increases in urine specific gravity and serum creatine phosphokinase activity were not accompanied<br/> with related signs such as histopathological changes or clinical findings. In conclusion, four<br/> week repeated oral dose of DFA IV to rats did not cause apparent toxicological change at the dose of<br/> 500, 1,000 or 2000 mg/kg body weight. Thus it is suggested that no-observed-adverse-effect level<br/> (NOAEL) of DFA IV in rats would be 2,000 mg/kg/day body weight.