連文複音詞 “歡喜/喜歡” 研究

胡玉華(Hu Yuhua) 동양한문학회(구 부산한문학회) 2019 동양한문학연구 Vol.52 No.-

“喜歡(희환)”과 “歡喜(환희)”는 동소역순어(同素逆序)어로서 모두 ‘희열, 기쁨’과 ‘좋아하다’라는 의미를 지니고 있다. 본 논고에서는 이 두 단어에 대한 통시적인 고찰을 통하여 그 의미 변화를 분석하고 사용빈도를 통계하였으며, 사용빈도에서 나타나는 차이 점의 원인을 분석하였다. 동시에 이 두 단어가 普通話(표준어)와 방언에서 어떻게 전승되어 왔는가를 고찰하였다. Xihuan and Huanxi“ are a pair of the inverse morphemes words with the semantics of “happy”and“like”. This study researches the two words by diachronic research method, describes their semantic changes, and analyzes the reasons why these two words is different in frequency when used in ancient literatures. At last, this study analyzes the inheritance of the two words in modern Chinese Mandarin and dialects.

Genome shuffling improved acid-tolerance and succinic acid production of Actinobacillus succinogenes

Shumeng Hu,Ying You,Feifei Xia,Junmei Liu,Weichang Dai,Jingsheng Liu,Yuhua Wang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3

Succinic acid is widely applied to chemical,pharmaceutical, food, and agricultural industries. With therapid development of these industries, a great demand ofsuccinic acid is required. The acid-tolerance and succinicacid production of Actinobacillus succinogenes strain wereimproved by using genome shuffling. Results showed thatone modified strain AS-F32, with the best acid resistanceand the highest succinic acid production, was obtained after3 cycles of genome shuffling. The minimum growth pH ofAS-F32 was 3.5, and the acid production and cell dryweight were 5.1 and 4.8 g/L in flask, improved 2.6 and1.85 times over the start strain As-R2. Furthermore, thesuccinic acid yield of As-32 was 31.2 g/L and the dry cellweight was increased 44.4% by maintaining pH 4.8 with7.0 M NH4OH in 5 L bioreactor, increased 1.1 times thanthe original strain As-R2.

High performance proton-conducting solid oxide fuel cells with a layered perovskite GdBaCuCoO5+x cathode

Xiaozhen Zhang,Yuhua Jiang,Xuebing Hu,Liangliang Sun,Yihan Ling 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.2

Proton-conducting solid oxide fuel cell (H-SOFC) based on layered perovskite type GdBaCuCoO5+x (GBCC) cathodewas fabricated with in situ drop-coating BaZr0.1Ce0.7Y0.2O3−δ (BZCY) electrolyte membrane. The influences of Cu dopinginto Co sites of GdBaCo2O5+x on the electrical conductivity and conduction mechanism, thermal expansion propertyand electrochemical performance of cathode materials and corresponding single cell were investigated. Results show thatthe electrical conductivity decreased and the conduction mechanism would gradually transform to the semiconductor-likebehavior. A high maximum power density of 480 mW cm−2 was obtained for the anode supported NiO–BZCY/NiO–BZCY/BZCY/GBCC single cells with wet H2fuel at 700 °C. The corresponding polarization resistance was as low as 0.17 Ω cm2. The excellent electrochemical performance of as-prepared single cell indicates that GBCC is a good candidate of cathodematerials for H-SOFCs.

An Optimized Third Harmonic Injection Method for Three-Phase Cascaded H-Bridge Photovoltaic Inverter

Wang Mao,Xing Zhang,Tao Zhao,Yuhua Hu,Fusheng Wang,Zhiqiang Dai,Renxian Cao 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The uneven irradiation and temperature in photovoltaic (PV) modules of three-phase cascaded H-bridge (CHB) inverters will cause the unbalanced output power between solar panels, resulting in unbalanced grid current between three phases. In addition, since each phase can be regarded as a single-phase inverter, the second harmonic voltage pulsation of dc-side capacitors is a problem to be solved. To solve these two problems, a third harmonic injection optimization method on the basis of fundamental frequency zero sequence is proposed in this paper. The third harmonic with the same amplitude of fundamental modulated wave signal is injected into the modulated wave signal when the amplitude of synthesized wave signal is no greater than unity. Otherwise, the amplitude of third harmonic is decreased correspondingly to ensure the amplitude of synthesized wave signal being unity. With this method, the dc-side voltage pulsation is effectively restrained and the power balancing limit of CHB inverter is expanded. The feasibility and effectiveness of this method are verified by an experimental prototype of a three-phase seven-level CHB inverter.

Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis

Yixin Wang,Long Jin,Jideng Ma,Li Chen,Yuhua Fu,Keren Long,Silu Hu,Yang Song,Dazhi Shang,Qianzi Tang,Xun Wang,Xuewei Li,Mingzhou Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. Methods: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. Results: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Conclusion: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.

Biological characterization of mesenchymal stem cells from bovine umbilical cord

Hui Xiong,Wei Jun Guan,Yue Hui Ma,Chunyu Bai,Shuang Wu,Yuhua Gao,Taofeng Lu,Qingyun Hu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.1

Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into othercell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, andavian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle asthe experimental animal, we describe an external culture of the UCMSCs from it and summarize the biologicalcharacteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, geneexpression, and differentiation potential were detected via using immunofluorescence and reverse transcriptionpolymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, andneurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, andDithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics ofthe UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured topassage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustratedtypical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternativesource of MSCs for experimental and clinical applications.

Ginsenosides as dietary supplements with immunomodulatory effects: a review

Tang Ping,Liu Sitong,Zhang Junshun,Ai Zhiyi,Hu Yue,Cui Linlin,Zou Hongyang,Li Xia,Wang Yu,Nan Bo,Wang Yuhua 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Immune disorders have become one of the public health problems and imposes a serious economic and social burden worldwide. Ginsenosides, the main active constituents of ginseng, are regarded as a novel supplementary strategy for preventing and improving immune disorders and related diseases. This review summarized the recent research progress of ginsenosides in immunomodulation and proposed future directions to promote the development and application of ginsenosides. After critically reviewing the immunomodulatory potential of ginsenosides both in vitro and in vivo and even in clinical data of humans, we provided a perspective that ginsenosides regulated the immune system through activation of immune cells, cytokines, and signaling pathways such as MAPK, PI3K/ Akt, STAT, and AMPK, as well as positively affected immune organs, gut flora structure, and systemic inflammatory responses. However, the evidence for the safety and efficacy of ginsenosides is insufficient, and the immune pathways of ginsenosides remain incompletely characterized. We believe that this review will provide a valuable reference for further research on ginsenosides as dietary supplements with immunomodulatory effects.

MiR-200c-3p inhibits LPS-induced M1 polarization of BV2 cells by targeting RIP2

Zhao Lei,Liu Xiaosong,Yang Jiankai,Wang Xiaoliang,Liu Xiaomeng,Wu Jianliang,Li Chen,Xu Donggang,Hu Yuhua 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Background: Microglia are important immune cells, which can be induced by lipopolysaccharide (LPS) into M1 phenotype that express pro-inflammatory cytokines. Some studies have shown that microRNAs play critical roles in microglial activation. Objective: This study was designed to investigate the role of miR-200c-3p in regulating inflammatory responses of LPS-treated BV2 cells. Methods: The expression of miR-200c-3p in BV2 cells was detected by real-time PCR. Receptor-interacting protein 2 (RIP2) was predicted as a target gene of miR-200c-3p. Their relationship was verified by dual-luciferase reporter assay. The function of miR-200c-3p and RIP2 in microglial polarization and NF-κB signaling was further evaluated. Results: LPS treatment reduced miR-200c-3p expression in a dose-dependent and time-dependent manner in BV2 cells. LPS treatment increased the expression of M1 phenotype markers inducible nitric oxide synthase (iNOS) and major histocompatibility complex class (MHC)-II, promoted the release of pro-inflammatory cytokines interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, and enhanced the nuclear translocation and phosphorylation of nuclear factor-kappaB (NF-κB) p65. Reversely, miR-200c-3p mimics down-regulated the levels of these inflammatory factors. Furthermore, RIP2 was identified to be a direct target of miR-200c-3p. RIP2 knockdown had a similar effect to miR-200c-3p mimics. Overexpression of RIP2 eliminated the inhibitory effect of miR-200c-3p on LPS-induced M1 polarization and NF-κB activation in BV2 cells. Conclusions: MiR-200c-3p mimics suppressed LPS-induced microglial M1 polarization and NF-κB activation by targeting RIP2. MiR-200c-3p/RIP2 might be a potential therapeutic target for the treatment of neuroinflammation-associated diseases.