Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis

Yixin Wang,Long Jin,Jideng Ma,Li Chen,Yuhua Fu,Keren Long,Silu Hu,Yang Song,Dazhi Shang,Qianzi Tang,Xun Wang,Xuewei Li,Mingzhou Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. Methods: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. Results: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Conclusion: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.

Exploring the Relation of Smartphone Addiction and Musculoskeletal Pain in the Neck, Trunk, and Upper Limbs: A Cross-sectional Study

Yixin Wang,Ye-Jin Kim,Kyeong-Ah Moon,Joo-Hee Park,Hye-Seon Jeon 한국전문물리치료학회 2023 한국전문물리치료학회지 Vol.30 No.3

Background: Smartphone addiction has emerged as a significant social problem. Numerous studies have indicated the association between smartphone use and discomfort in the muscu-loskeletal system of the upper extremities. Objects: This cross-sectional survey aimed to compare the characteristics of musculoskeletal pain in the neck, trunk, and upper limbs between individuals with smartphone addiction and those without addiction. Methods: We collected a total of 326 healthy individuals’ data from China and Korea who had owned and used smartphones for more than 5 years between 20–50s through an online questionnaire consisting of 84 questions in four major sections. The first part contained basic information on the participant's personal characteristics and smartphones. The second part contained questions about smartphone use and posture. The third part was the smartphone addiction. The fourth part was to investigate musculoskeletal pain in various upper body parts. Results: Smartphone addiction has a weak negative correlation with age (r = –0.20, p < 0.01) and a weak positive correlation with the hours of smartphone use (r = 0.376, p < 0.01). Frequent musculoskeletal pain symptoms related to smartphone use were observed in the neck, shoulder, lower back, and wrists. The hours of smartphone use was slightly positively associated with the prevalence of musculoskeletal pain in the shoulder (r = 0.162, p < 0.05) and lower back (r = 0.125, p < 0.05). The prevalence of musculoskeletal pain in the neck (χ2 = 3.993, p < 0.05), shoulder (χ2 = 6.465, p < 0.05), and wrist (χ2 = 4.645, p < 0.05) was significantly higher among females than males. Conclusion: The results suggest that smartphone addiction should be recognized as a dual concern encompassing both physical health and psychosocial aspects. Furthermore, health-care professionals, including physicians and physical therapists, should consider clients' smart-phone usage patterns when assessing and treating with musculoskeletal pain.

Correlation Between Knee Muscle Strength and Maximal Cycling Speed Measured Using 3D Depth Camera in Virtual Reality Environment

Ye Jin Kim(Ye Jin Kim),Hye-seon Jeon(Hye-seon Jeon),Joo-hee Park(Joo-hee Park),Gyeong-Ah Moon(Gyeong-Ah Moon),Yixin Wang(Yixin Wang) 한국전문물리치료학회 2022 한국전문물리치료학회지 Vol.29 No.4

Background: Virtual reality (VR) programs based on motion capture camera are the most convenient and cost-effective approaches for remote rehabilitation. Assessment of physical function is critical for providing optimal VR rehabilitation training; however, direct muscle strength measurement using camera-based kinematic data is impracticable. Therefore, it is necessary to develop a method to indirectly estimate the muscle strength of users from the value obtained using a motion capture camera. Objects: The purpose of this study was to determine whether the pedaling speed converted using the VR engine from the captured foot position data in the VR environment can be used as an indirect way to evaluate knee muscle strength, and to investigate the validity and reliability of a camera-based VR program. Methods: Thirty healthy adults were included in this study. Each subject performed a 15-second maximum pedaling test in the VR and built-in speedometer modes. In the VR speedometer mode, a motion capture camera was used to detect the position of the ankle joints and automatically calculate the pedaling speed. An isokinetic dynamometer was used to assess the isometric and isokinetic peak torques of knee flexion and extension. Results: The pedaling speeds in VR and built-in speedometer modes revealed a significantly high positive correlation (r = 0.922). In addition, the intra-rater reliability of the pedaling speed in the VR speedometer mode was good (ICC [intraclass correlation coefficient] = 0.685). The results of the Pearson correlation analysis revealed a significant moderate positive correlation between the pedaling speed of the VR speedometer and the peak torque of knee isokinetic flexion (r = 0.639) and extension (r = 0.598). Conclusion: This study suggests the potential benefits of measuring the maximum pedaling speed using 3D depth camera in a VR environment as an indirect assessment of muscle strength. However, technological improvements must be followed to obtain more accurate estimation of muscle strength from the VR cycling test.

A Virtual Resistance Based Reactive Power Sharing Strategy for Networked Microgrid

Yixin Zhu,Baoquan Liu,Feng Wang,Fang Zhuo,Yangjie Zhao 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Different from the traditional microgrid with a common ac bus, networked microgrid always suffers more serious reactive power sharing problems due to its complex inner configurations. In such a situation, the reactive power sharing errors among distributed generation (DG) units can’t be eliminated effectively. Thus, an advanced reactive power sharing strategy that employs communication and virtual resistance control is hereby proposed for networked microgrid. First of all, the relation between reactive power output and virtual impedance regulation (VRR) is discussed. After that, communication is introduced to assign reactive power reference to each DG unit for their respective VRR, which is able to compensate the mismatch in network. The method is immune to the load change during the regulation stage, and also to the time delay in communication channels. From the small signal analysis, it also can be seen that in the designed regulation range, VRR has no significant effect on the system stability. The feasibility and effectiveness of the proposed strategy are validated by the simulation and real time digital simulator (RTDS) test results from a 50 kVA networked microgrid system.

Solubility of D-p-hydroxyphenylglycine p-toluenesulfonate and L-p-hydroxyphenylglycine in different solvents

Yixin Qu,Shui Wang,Tian Li 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.10

The solubility of D-p-hydroxyphenylglycine p-toluenesulfonate and L-p-hydroxyphenylglycine in different solvents was measured using a laser monitoring observation technique. The solubility data were accurately correlated by the modified Apelblat equation, the calculated results of which were proved to show fine representation of experimental data.

Reconstruction of a Volatility Based on the Black-Scholes Option Pricing Model Using Homotopy Perturbation Inversion Method

Yixin Dou,Jianhua Fu,Zhihao Wang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.9

The reconstruction of a volatility based on a Black-Scholes option pricing model is ill-posed. In order to overcome the ill-posedness, a homotopy perturbation inversion method is designed to solve the inverse problem. The proposed method is a modified version of the Landweber method. The reconstruction of a volatility is a nonlinear problem which is needed to be linearized. Hence, numerical experiments consist of the reconstruction of a policy parameter based on a Todaro model which is a linear inverse problem and the reconstruction of a volatility based on a Black-Scholes option pricing model in order to test the performance of the proposed method. Numerical examples show that the proposed method is more accurate and faster than the Landweber method.

A Novel Fixed Twin-Rotor Unmanned Aerial Vehicle with Variable Angle Louver Rudder

Yixin Zhang,Jianfeng Zhou,Shaoping Wang,Mengqi Yang,Shaoshi Li 제어로봇시스템학회 2021 제어로봇시스템학회 국제학술대회 논문집 Vol.2021 No.10

A novel fixed twin-rotor unmanned aerial vehicle (UAV) based on the variable angle louver rudder (VALR) is developed in this work. In view of the mechanical characteristics under the action of the louver rudder, specific kinematics and dynamics models are derived. The aerodynamic characteristics of the UAV are studied through computational fluid dynamics simulation, and a ground measurement system is designed to analyze the aerodynamic characteristics of the prototype. An attitude control model is proposed, and maneuvers such as take-off, landing, hovering, and braking are realized through flight experiments, which verify the feasibility of the prototype design. This research shows that the new layout with the VALR system, combined with the advantages of rudder control and vector control, can reduce the structural complexity and deadweight of the UAV while ensuring flight stability and improving maneuverability. Moreover, the novel UAV has higher energy efficiency and economy.

Comparative Study Based on Two Kinds of Mobile Ad Hoc Network Routing Protocols

Yixin Yan,Huamin Wang 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.11

A mobile ad hoc network is a special kind of wireless mobile communication network, featuring equal status of all terminals, ad hoc, no need for prior arrangement of any network facilities or central control nodes, dynamically changing topology and bandwidth and energy restriction. In recent years, research on mobile ad hoc networks, especially research on media access control and routing protocols have research focus. In this paper, the well-known on-demand routing protocol AODV is analyzed and is improved as AODV-CLMP, with a simulation platform used to evaluate its performance, with some meaningful conclusions drawn. In this paper, the origin, characteristics, application and current development of mobile ad hoc networks are introduced briefly, followed by research on two kinds of well-known on-demand routing protocols for mobile ad hoc networks, i.e. AODV and AODV-CLMP. Finally, commonly used network simulation software is compared to conduct evaluation and analysis of the performance of the AODV and AODV-CLMP protocols. Through the analysis of the simulation result of such evaluation indicators as average end-to-end delay, packet delivery fraction and throughput, the following conclusions are drawn: the average delay, packet deliveryfractionand throughput of AODV-CLMP protocol are superior to those ofAODV protocol, indicating thatAODV-CLMP protocol can transmit information more rapidly and steadily.

Hierarchical porous composites derived from spent tire char for CO2 capture: K-N co-doped, process intensification and thermodynamic analysis

Hongguan Wang,Guofeng Qiu,Yanjie Niu,Liqing Chen,Yan Li,Sixi Guo,Yixin Zhang,Jianjun Wu,Fanhui Guo 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

Spent tire is a kind of solid waste with large production, accumulation and great pollution. Spent tire charis the primary product of tire harmless disposal. Its commercial value is enhanced by modification. In thisstudy, K-N co-doped activated carbon was prepared by using spent tire char as raw material, KOH andmelamine as activator and dopant to enhance CO2 capture. In comparison with traditional process, thereasons for the decrease in specific surface area and pore volume caused by K doped have been identified,and a potential doped mechanism has been proposed. It was determined that pyridine nitrogen in theamino functional group played a key role in CO2 adsorption. Meanwhile, the synergistic intensificationof CO2 adsorption by potassium phenolic and pyridine nitrogen was verified by simplified model simulation. The precise K-N doping significantly intensify the CO2 adsorption capacity of spent tire char from0.45 to 2.60 mmol/g (1 bar, 0℃), an increase of 4.78 times. Thermodynamic studies have demonstratedthat the Langmuir model accurately describes the adsorption process, and the adsorption reaction takesplace spontaneously at temperatures below 80.52 C. This work presents an efficient process for upgradingspent tire char, and a novel method of co-treating waste and CO2.

Comprehensive Comparison and Evaluation of the WS , MHV2 , Twu and CWSM Mixing Rules

( Wenchuan Wang ),( Yixin Qu ) 한국화학공학회 1995 화학공학의이론과응용 Vol.1 No.1