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( Nguyen Hoai Nam ),박성훈 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
3-hydroxypropioinc acid (3-HP), an important platform chemical, can be produced from glycerol through a two-step reaction catalyzed by glycerol dehydratase (GDHt) and 3-hydroxypropionaldehyde dehydrogenase (ALDH). When these enzymes are highly expressed from multi-copy plasmid(s), recombinant microorganisms can produce 3-HP efficiently. However, for industrial purposes, the enzymes should be expressed from the chromosome. In this study, using the recentlydiscovered novel promoter which is induced by the target product 3-HP, expression cassette for a GDHt was developed. Gene expression was modified at transcriptional and translational levels, and the expression efficiency was evaluated by RT-PCR, protein production, and/or enzyme activity measurements. The GDHt expression/activity, under both non-induced and induced conditions, could be varied in a wide range by proper combination of the gene expression control methods.
A New Sterol from the Soft Coral Lobophytum crassum
Nguyen, Phuong Thao,Nguyen, Hoai Nam,Nguyen, Xuan Cuong,Nguyen, Xuan Nhiem,Pham, The Tung,Tran, Hong Quang,Nguyen, Thi Thanh Ngan,Phan, Van Kiem,Chau, Van Minh,Kim, Young Ho Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.1
Development of Biosensor for 3-Hydroxypropionic Acid
Nam Hoai Nguyen,Jung Rae Kim,SUNGHOON PARK 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.1
In this study, whole-cell biosensors that detect and indicate the concentration of 3-hydroxypropionic acid (3-HP) by producing a green fluorescent signal were developed using a transcription factor (TF)-responsive 3- HP inducible promoter identified in the Pseudomonas denitrificans 3-HP degradation pathway. Upon forming a complex with 3-HP, the MmsR TF protein, a LysR-type transcriptional regulator (LTTR), binds to the PmmsA promoter and controls the expression of the PmmsA-regulated gfp reporter gene in response to the concentration of 3-HP. Furthermore, by enhancing the expression of MmsR and through mutagenesis of the PmmsA promoter region, three well-performing biosensors were developed that covered a wide dynamic range of 3-HP (0.01-100 mM when added externally) with ~100-fold signal change upon induction in P. denitrificans. The 3-HP biosensor machinery, composed of MmsR, the PmmsA promoter and gfp could also function well in E. coli and P. putida. The developed 3-HP biosensors should be useful for engineering 3-HP-producing strains and the enzymes associated with its production.
Patchy approximate explicit model predictive control
Nguyen Hoai Nam,Sorin Olaru,Morten Hovd 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10
Multiparametric quadratic programming (MPQP) can be used to construct an off-line solution to constrained linear model predictive control. The result is a piecewise linear state feedback defined over polyhedral cells of the state space. However, with high dimensional problems, coding and implementation of this solution may be very burdensome for the available hardware, due to the high number of polyhedral cells in the state space partition. In this paper we provide an algorithm to find an approximate solution to MPQP, which is obtained by linear interpolation of the exact solution at the vertices of a feasible set and the solution of linear quadratic(LQ) problem. Based on a patchy control technique, we assure robust closed loop stability in the presence of additive measurement noise despite the presence of discontinuities at the switch between the regions in the state space partition.
Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor
Nam Hoai Nguyen,김중래,박성훈 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.5
Application of the recently developed wholecell 3-HP biosensor, which detects 3-hydroxypropionic acid (3-HP) and expresses fluorescence as an output signal in response to the 3-HP concentration, was studied in three areas of enzyme and metabolic engineering. First, a sensor was applied to identify active 3-hydroxyaldehyde dehydrogenase (ALDH), a key enzyme in the 3-HP production pathway. Second, with the aid of helper enzymes that catalyze the conversion of 1,3-propanediol (1,3-PDO) to 3-HP, a 3-HP biosensor was converted into a 1,3-PDO biosensor. Third, a 3-HP biosensor, with proper modifications in expression module of the output signal and the use of pH-tolerant red fluorescent protein (RFP), was shown to monitor the rate of 3-HP production under process conditions in which one or more interfering compounds are present in the culture medium and/or the medium pH decreases. This study demonstrates that 3-HP biosensors can be widely used in enzyme and metabolic engineering applications for 3-HP production.
Nguyen Ba Tiến,Hoai-Nam Nguyen,Hoang-Ha Le,Tran Thu Trang,Chau Van Dinh,Ha-Nam Nguyen,Gyoo Seok Choi 한국인터넷방송통신학회 2023 International Journal of Internet, Broadcasting an Vol.15 No.2
A common approach to the problem of predicting student test scores is based on the student's previous educational history. In this study, high school transcripts of about two thousand candidates, who took the High-school Student Assessment (HSA) were collected. The data were estimated through building a regression model - Random Forest and optimizing the model's parameters based on Genetic Algorithm (GA) to predict the HSA scores. The RMSE (Root Mean Square Error) measure of the predictive models was used to evaluate the model’s performance.
Nguyen Phuong Thao,Nguyen Hoai Nam,Nguyen Xuan Cuong,Bui Thi Thuy Luyen,Bui Huu Tai,김지은,송석빈,Phan Van Kiem,Chau Van Minh,김영호 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.6
Anti-inflammatory transcriptional effects ofnineteen compounds (1–19) from the soft coral Sinulariamaxima were evaluated using NF-jB luciferase and reversetranscriptase polymerase chain reaction. Compounds 1, 2,4, 8, 15, 17, and 18 significantly inhibited TNFa-inducedNF-jB transcriptional activity in HepG2 cells in a dosedependentmanner, with IC50 values ranging from15.81 ± 2.29 to 29.10 ± 1.54 lM. Furthermore, the transcriptionalinhibitory function of these compounds wasconfirmed by a decrease in intercellular adhesion molecule-1 and inducible nitric oxide synthase gene expressionlevels in HepG2 cells. These results provide a scientificrationale for the use of the soft coral S. maxima warrantfurther studies to develop new agents for the preventionand treatment of inflammatory.
Nguyen Phuong Thao,Bui Thi Thuy Luyen,Chau Ngoc Diep,Bui Huu Tai,김은지,강희경,이상현,장해동,Nguyen The Cuong,Nguyen Van Thanh,Nguyen Xuan Cuong,Nguyen Hoai Nam,Chau Van Minh,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4
This study performed phytochemical and bioactiveassessments of the mangrove Lumnitzera racemosaWilld. leaves. Bioassay-guided fractionation of the methanolicextracts led to the identification of thirty-six compounds(1–36), their structures were elucidated using detailed NMRspectroscopic and MS analysis. The extracts, fractions, andthe isolated compounds were screened for potential antioxidantand cytotoxic activities. Antioxidant assays wereperformed using peroxyl radical-scavenging and reducingassays, whereas cytotoxicity was measured using MTTassays in HL-60 and Hel-299 cell lines. The methanolicextract, CH2Cl2 and n-BuOH fractions (10.0 lg/mL)exhibited potent antioxidant activity, with Trolox equivalent(TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and27.09 ± 0.37 (lM), respectively. In addition, the isolatedcompounds exerted cytotoxic effects in a dose-dependentmanner; compounds 1 and 14 exhibited the most potentcytotoxicity in HL-60 cells, with IC50 values of 0.15 ± 0.29and 0.60 ± 0.16 lM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured thetime-dependent changes in apoptotic markers including thecondensation and fragmentation of nuclear chromatin, andthe downregulation of p-ERK1/2, p-AKT, and c-Myc levels.