Ecological characteristics of areas naturally inhabited by Dolbae trees(Pyrus pyrifolia) on Mt. Kaya

Ahn, Young-Hee,Chung, Kyu Hwan 한국환경과학회 2002 한국환경과학회지 Vol.11 No.11

The ecological characteristics of Dolbae trees, a valuable genetic resource for native Pyrus fruit trees, were investigated at four naturally inhabited areas on Mount Kaya, Gyeongsangnam-Do. Most Dolbae trees were found on the southwest side of an old hiking path to a mountain valley at altitudes of 610~670 m. The surrounding flora consisted of 46 families, 69 genera, 75 species, and 10 varieties. The natural habitat of the Dolbae trees was basically a deciduous broad-leaved forest with a predominance of Quercus serrata and ligneous plants(xylophytes) from the Fagaceae and Betulaceae families. The habitat was also found to be in a second transition resulting from forest disturbance and exhibited a degree of diversity of 2.901-5.065, based on Shannon-Weiner's index. Examination of the Dolbae trees found in Mount Kaya revealed diameter breast heights(DBHs) ranging from 10 to 60 cm, including six old and large Dolbae trees with a DBH of 50~60 cm, which will be particularly valuable as a genetic resource. Accordingly, the current results suggest that the study areas are a secondary forest in a typical mid-temperate zone resulting from forest damage about 10 years earlier.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

유아의 기질과 어머니의 성격특성 및 부모의 양육일치도가 어머니의 양육스트레스에 미치는 영향

안영희(Young Hee Ahn) · 김연화(Youn Hwa Kim) · 한세영(Sae Young Han) 충북대학교 생활과학연구소 2008 생활과학연구논총 Vol.12 No.2

The purpose of this research was to uncover the relative impact of children's temperament, mothers' characteristics, and parents' child-rearing agreement on mother's parenting stress. Subjects of this study were mothers of 293 preschoolers, being aged 3 to 5 years old from five kindergartens placed in Ahn-yang city and Em-sung gun, Chungbuk. Data were analyzed with Pearson's correlation and heirarchical Regression by using SPSS program. The results showed that children's emotional sensitivity and mother's neuroticism had a positive impact on mother's parenting stress and mother's extroversion had a negative impact on mother's parenting stress. Moreover, couple's child-rearing agreement showed a negative influence on mother's parenting stress.

용해실리카의 기준경도를 이용한 나노 압입시스템의 실험적 교정

이윤희 ( Yun Hee Lee ),안승민 ( Seung Min Ahn ),김주영 ( Ju Young Kim ),박찬평 ( Chan Pyung Park ),장희광 ( Hee Kwang Jang ) 대한금속재료학회(구 대한금속학회) 2015 대한금속·재료학회지 Vol.53 No.3

Indirect calibration of a nanoindentation system was performed by deriving a reference loading curve. This reference load-depth relationship was accomplished by assuming a perfect nanoindentation test on an ideal reference material; if all components in a nanoindentation system are well calibrated and the reference material has unique hardness and Young’s modulus, the indentation depth can be formulated with a function of the indentation load having two parts of contact and surrounding elastic depths. Substituting both the hardness and Young’s modulus of a fused silica into the derived function, the reference loading curve was plotted and directly compared with experimental data of the fused silica measured by two commercial nanoindenters. All indentation loading curves showed leftward shifts compared to the reference loading curve, and this is discussed, offering two reasons for the result, system compliance and indenter apex geometry.(Received January 6, 2014)

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

전통누룩 진균류를 이용한 입국의 제조 및 입국곰팡이의 동정

김재호 ( Jae Ho Kim ),권영희 ( Young Hee Kwon ),이애란 ( Ae Ran Lee ),김혜련 ( Hye Ryun Kim ),안병학 ( Byung Hak Ahn ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

다양한 향미를 가진 막걸리의 개발을 위해 전통누룩으로부터 분리한 곰팡이로 입국을 제조한 후 품질특성을 분석하여 입국의 규격에 적합하며 이취가 없고 관능이 우수한 9균주를 입국 제조용 우수균주로 최종 선발하였다. 선발된 균주는 Aspergillus oryzae(C1-5-2-2, C20-7-3, CN1.3.1-4, CN16.19.1-1, N152-1, N220-1), Mycocladus corymbiferus (N162-2), Rhizopus oryzae(N20), Lichtheimia corymbifera (N21)로 동정되었으며, 제조한 입국의 산도는 5.0~6.8, 당화력은 128~241sp이었다. Various koji were prepared by fungi isolated from traditional nuruk and their quality characteristics were investigated. Acidity and saccharification power of their koji were ranged in 5.0~6.8 and 128sp~241sp. Nine fungi which were showed good quality and sensory evaluation were identified by analysis of their nucleotide sequences with PCR-amplified 18S rDNA internal transcribed spacer-1(ITS-1) and ITS-4 genes. Among them, six strains were identified as Aspergillus oryzae and the other strains were identified as Mycocladus corymbiferus, Rhizopus oryzae, Lichtheimia corymbifera.

관광객의 쇼핑가치 지각이 쇼핑만족에 미치는 영향에 관한 연구

안대희,김희영 세종대학교 관광산업연구소 2002 호텔관광경영연구 Vol.17 No.-

The objectives of this study were 1) to examine the relationship between shopping value and shopping satisfaction.; 2) to examine the relationship between store type and shopping value. Self-administrated questionnaire was used to collect the data for this study after reviewing previous studies and conducting pre-test twice. The data was analyzed by using analysis, Multiple regression, T-test. The followings are the results of this study, First, There were significant relationships between Tourists' Shopping value and Shopping satisfaction. Second, Store type were significant differences according to Tourists' Shopping value. The result of this study will be helpful for the shopping industries which gave needs to establish the marketing strategies.

부티크호텔('Boutique Hotel')과 실내디자인 : 로비공간을 중심으로

안희영 건국대학교 조형연구소 2003 조형연구 Vol.11 No.-

The Boutique Hotels have been rapidly growing since the end of the 20th century, breaking away from the conventional chain and standardized hotel system, raising customer satisfaction, introducing new concepts and providing customers with something to see and to enjoy. The conventional hotel is suggestive of such hotel space containing magnificent and deluxe convenience facilities, standardized furniture and identical symbols and logos and complete with commonly expected functions but the Boutique Hotels might be described as making inroads into niche market. providing subjects of gossips, being rather a little clumsy and presenting sort of customer-tailored conveniences at the expense of multi-functional conveniences. With saloon-type boutique cultural elements such as design, art or saloon-type added, the Boutique hotel may well produce new paradigms. Interior design, in particular, gives so direct impact on the feeling of customers and plays such an important role for the space that in most of the hotels the interior design is considered as important as the services provided. Therefore, by analyzing such research and cases on the Boutique hotels, we may redefine our hotel culture which is excessively Western-elements oriented as the proper hotel culture where we may enjoy healthy and comfortable leisure, breaking away from the notion that the tourist hotels must be mammoth-sized and motels should accommodate only part of the functions performed by mammoth hotels.

비완압형 어태치먼트의 위치에 따른 하악 가철성 국소위치의 지지조직에서의 저작압 분산 양태에 관한 3차원 유한요소적 연구

안원준,우순섭,이영수,심광섭,유광희 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1

The purpose of this study was to evaluate the stress patterns developed in supporting structures by removable partial denture with an extracoronal and an intracoronal rigid attachment. The mandibular removable partial dentures with Mini S-G (extracoronal) and C-M Box (intracoronal) attachment were constructed. Under 100N, 200N of vertical and 25N, 50N of lateral loads at the 1st and 2nd lower molar, stress distribution patterns and displacement were analyzed with three dimensional finite element method. The result were as follows: 1. There was no apparent difference in the patterns of stress distribution between rigid extracoronal and rigid intracironal attachments. 2. In case of the single abutment tooth with the rigid intracoronal attachment, it was not proper to preserve the abutment tooth. 3. With splinted abutments, the stress was distributed through all the root surface of both abutments. With single abutment, the stress was concentrated on the distal root surface of abutment. 4. With splinted abutments, there was not clear difference in the patterns of stress distribution with or without lingual bracing part. 5. In case of the single abutment tooth with the rigid intracoronal attachment and cantilever. More stress was concentrated around the abutment tooth with the lingual bracing part under loaded concentrated force and the abutment tooth without the lingual bracing part under loaded distributed force. 6. In case of the splinted abutments with the rigid extracoronal and intracoronal attachment, the denture base was displaced posteriorly and inferiorly when the force was applied. 7. In case of the single abutment tooth with the rigid intracoronal attachment, there was little displacement at the denture base.