Molecular Cloning, Identification and Characteristics of a Novel Isoform of Carbamyl Phosphate Synthetase 1 in Human Testis

( Ran Huo ),( Hui Zhu ),( Li Lu ),( Lan Lan Ying ),( Min Xu ),( Zhi Yang Xu ),( Jian Min Li ),( Zuo Min Zhou ),( Jia Hao Sha ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.1

A gene coding a novel isoform of carbamyl phosphate synthetase I (CPSI) was cloned from a human testicular library. As shown by cDNA microarray hybridization, this gene was expressed at a higher level in human adult testes than in fetal testes. The full length of its cDNA was 3831 bp, with a 3149 bp open reading frame, encoding a 1050-amino-acid protein. The cDNA sequence was deposited in the GenBank (AY317138). Sequence analysis showed that it was homologous to the human CPS1 gene. The putative protein contained functional domains composing the intact large subunit of carbamoyl phosphate synthetase, thus indicated it has the capability of arginine biosynthesis. A multiple tissue expression profile showed high expression of this gene in human testis, suggesting the novel alternative splicing form of CPS1 may be correlated with human spermatogenesis.

A GPU-Accelerated Hydrodynamic Model for Urban Rainstorm Inundation Simulation: A Case Study in China

Hao Han,Jingming Hou,Zongxue Xu,Haixiao Jing,Jiahui Gong,Depeng Zuo,Bingyao Li,Shaoxiong Yang,Yongde Kang,Run Wang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.3

Frequent urban rainstorm inundations can cause serious damages to human life and social economy. Reliable simulation of urban rainstorm inundation is an effective approach for performing flood risk analysis to reduce losses. In this work, a full 2D high-performance hydrodynamic model for urban rainstorm inundation simulation based on Graphic Processing Unit (GPU) was developed. The idealized V-shape catchment and sponge city district of Fengxi New City were selected as the study areas to assess performance of the model. The model was validated against analytical benchmark results of the idealized V-catchment test which show good agreement between the modelled flow and analytical solutions. The model was then applied to simulate actual urban rainstorm inundation process under measured rainfall. The results indicated that the model can be applied to high-resolution urban region simulations. Specifically, the validation results indicated that the proposed model had a good performance, with an accepted error of less than 15%. When applied to compute the Fengxi New city under design rainstorms with different annual return periods of 1, 5, 10, 20, 50, and 100 years, it was found that the model effectively evaluated the temporal and spatial variation process of urban inundation, and quantitatively investigated flood risks according to the water depth change. The model has been substantially accelerated on GPU to quickly predict urbaninundation. The accuracy and rapid simulation speed of the model were verified based on the actual study area. Therefore, the proposed model can help to predict the dynamic processes of urban flood inundation and thus reduce flood inundation disasters. This is essential for future optimal sponge urban construction planning in China.

Comparative Study of DC Breakdown and Space Charge Characteristics of Insulation Paper Impregnated with Natural Ester and Mineral Oil

Hao, Jian,Zou, Run-Hao,Liao, Rui-Jin,Yang, Li-Jun,Liao, Qiang,Zhu, Meng-Zhao The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.4

Natural ester is a suitable substitute for mineral oil and has been widely used in AC transformer in many countries. In order to further application of natural ester in direct current (DC) equipment, it is needed to investigate its long term insulation property under DC condition. In this paper, a thermal ageing experiment was conducted for both mineral oil-paper and natural ester-paper insulation. The DC breakdown and space charge characteristics of insulation paper impregnated with natural ester and mineral oil was compared. Results show that the resistivity of the paper immersed in natural ester and mineral oil both increase as the ageing goes on. While insulation paper impregnated with natural ester has higher resistivity and DC breakdown voltage than the paper impregnated with mineral oil. The DC breakdown voltage for the oil impregnated insulation paper being DC pre-stressing is higher than that without pre-stressing. The average DC breakdown field strength difference between the test with pre-stressing and without pre-stressing clearly shows that there is an apparent enhancement effect for the homo-charge injection on the DC breakdown.

G-protein Coupled Estrogen Receptor 1 Expression in Primary Breast Cancers and Its Correlation with Clinicopathological Variables

Hao-jun Luo,Ping Luo,Guang-lun Yang,Qiong-le Peng,Man-ran Liu,Gang Tu 한국유방암학회 2011 Journal of breast cancer Vol.14 No.3

Purpose: G-protein coupled estrogen receptor 1 (GPER) probably play important roles in the progression of breast cancer including endocrine therapeutic resistance. We evaluated GPER in primary breast cancers. Methods: Immunohistochemistry was used to detect GPER in paraffin-embedded tissues of primary breast cancers from 423 patients and GPER expression was correlated with clinicopathological factors. Results: GPER was expressed in 63.8% of specimens, coexpressed with estrogen receptor alpha (ERα) in 36.6% of tumors and was positive in 62.5% of the ERα-negative tumors. The expression of GPER had no relationship with the status of ERα, progesterone receptor and HER2. Although the expression of GPER was significantly inversely related with nodal status (p=0.045), no correlation between GPER expression and other clinicopathological variables (age, menstruation status, tumor size, stage, histologic grade, Nottingham Prognostic Index or pathological type) was found. Conclusion: GPER and ERα exhibited independent expression pattern of distribution in primary breast cancers. A long-term follow-up and a more definite molecular phenotype for ER are necessary in confirming studies.

Comparative Study of DC Breakdown and Space Charge Characteristics of Insulation Paper Impregnated with Natural Ester and Mineral Oil

Jian Hao,Run-Hao Zou,Rui-Jin Liao,Li-Jun Yang,Qiang Liao,Meng-Zhao Zhu 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.4

Natural ester is a suitable substitute for mineral oil and has been widely used in AC transformer in many countries. In order to further application of natural ester in direct current (DC) equipment, it is needed to investigate its long term insulation property under DC condition. In this paper, a thermal ageing experiment was conducted for both mineral oil-paper and natural ester-paper insulation. The DC breakdown and space charge characteristics of insulation paper impregnated with natural ester and mineral oil was compared. Results show that the resistivity of the paper immersed in natural ester and mineral oil both increase as the ageing goes on. While insulation paper impregnated with natural ester has higher resistivity and DC breakdown voltage than the paper impregnated with mineral oil. The DC breakdown voltage for the oil impregnated insulation paper being DC prestressing is higher than that without pre-stressing. The average DC breakdown field strength difference between the test with pre-stressing and without pre-stressing clearly shows that there is an apparent enhancement effect for the homo-charge injection on the DC breakdown.

Comparison of carcass and meat quality traits between lean and fat Pekin ducks

Ding, Si-Ran,Li, Guang-Sheng,Chen, Si-Rui,Zhu, Feng,Hao, Jin-Ping,Yang, Fang-Xi,Hou, Zhuo-Cheng Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: According to market demand, meat duck breeding mainly includes 2 breeding directions: lean Pekin duck (LPD) and fat Pekin duck (FPD). The aim of the present study was to compare carcass and meat quality traits between 2 strains, and to provide basic data for guidelines of processing and meat quality improvement. Methods: A total of 62 female Pekin ducks (32 LPDs and 30 FPDs) were slaughtered at the age of 42 days. The live body weight and carcass traits were measured and calculated. Physical properties of breast muscle were determined by texture analyzer and muscle fibers were measured by paraffin sections. The content of inosine monophosphate (IMP), intramuscular fat (IMF) and fatty acids composition were measured by high-performance liquid chromatography, Soxhlet extraction method and automated gas chromatography respectively. Results: The results showed that the bodyweight of LPDs was higher than that of FPDs. FPDs were significantly higher than LPDs in subcutaneous fat thickness, subcutaneous fat weight, subcutaneous fat percentage, abdominal fat percentage and abdominal fat shear force (p<0.01). LPDs were significantly higher than FPDs in breast muscle thickness, breast muscle weight, breast muscle rate and breast muscle shear force (p<0.01). The muscle fiber average area and fiber diameter of LPDs were significantly higher than those of FPDs (p<0.01). The muscle fiber density of LPDs was significantly lower than that of FPDs (p<0.01). The IMF of LPDs in the breast muscle was significantly higher than that in the FPDs (p<0.01). There was no significant difference between the 2 strains in IMP content (p>0.05). The polyunsaturated fatty acid content of LPDs was significantly higher than that of FPDs (p<0.01), and FPDs had higher saturated fatty acid and monounsaturated fatty acid levels (p<0.05). Conclusion: Long-term breeding work resulted in vast differences between the two strains Pekin ducks. This study provides a reference for differences between LPD and FPD that manifest as a result of long-term selection.

Diversity and Metal Tolerance of Nematode-Trapping Fungi in Pb-Polluted Soils

Ming-He Mo,Wei-Min Chen,Hao-Ran Yang,Ke-Qin Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.1

The diversity of nematode-trapping fungi (NTF) in two lead (Pb) mines in Yunnan Province, China was investigated in 2004. In total, 20 species belonging to five genera were identified from 500 samples collected at the Lanping and the Huize mines. Pb concentrations ranged from 216~7,150 mg/kg for the former and 132~13,380 mg/kg for the latter, respectively. The fungi were divided into five groups based on different trapping mechanisms. The trapping-net producer group contained the largest number of species, with nine. Two predators, Dactylellina ellipsosporum and Arthrobotrys oligospora, were found at frequencies of 32.85% and 15.41%, respectively. The diversity indexes of NTF were positively correlated with Pb pollution levels in both the Lanping Mine (r=0.66) and the Huize Mine (r=0.72), suggesting that the distribution of NTF was not negatively affected by Pb contamination. For most strains of a given species, there was no significant difference (P>0.01) in the Pb tolerance between the strains isolated from habitats with low or high Pb concentrations. However, Pb toxicity exerted adverse effects on trap formation and predacious capability of fungi. We discuss the possible metal tolerance mechanisms and their relationships to the survival strategy of NTF in Pb-polluted environments.

Osteopontin Promotes the Development of Natural Killer Cells from Hematopoietic Stem Cells

Chung, Jin Woong,Kim, Mi Sun,Piao, Zheng-Hao,Jeong, Mira,Yoon, Suk Ran,Shin, Nara,Kim, Sang Yong,Hwang, Eun Sook,Yang, Young,Lee, Young Ho,Kim, Young Sang,Choi, Inpyo Wiley (John WileySons) 2008 Stem Cells Vol.26 No.8

<P>The detailed mechanisms driving the development of natural killer (NK) cells from hematopoietic stem cells remain to be clearly elucidated. Here, we show that osteopontin (OPN) is a key factor for NK development. OPN-deficient mice evidenced severe impairments of NK development in bone marrow (BM) and spleen in which the NK populations that express CD122 and NK cell receptors were reduced. However, the absence of intrinsic OPN expression did not affect NK development, whereas the absence of OPN in the microenvironment caused a significant reduction in NK population. The expression of OPN was induced by interleukin (IL)-15 in BM stromal cells, and the defect in NK differentiation in IL-15(-/-) hematopoietic precursor cells (HPC) was recovered by addition of recombinant OPN, suggesting that the microenvironmental OPN may be a key factor in IL-15-mediated NK differentiation. In addition, OPN-driven NK maturation was reduced in T-bet-deficient HPC, suggesting that T-bet is required for OPN-mediated NK development. Collectively, these results show that paracrine OPN signaling drives NK-lineage commitment, thus ultimately promoting NK cell development. Disclosure of potential conflicts of interest is found at the end of this article.</P>

Nano-scale SiO2 patterned n-type GaN substrate for 380 nm ultra violet light emitting diodes.

Jo, Min Sung,Seo, Hyo Won,Tawfik, Wael Z,Yang, Seung Bea,Lee, Jung Ju,Ryu, Sang Wan,Ha, Jun Seok,Jeon, Seong Ran,Cui, Hao,Park, Si Hyun,Lee, June Key American Scientific Publishers 2014 Journal of Nanoscience and Nanotechnology Vol.14 No.8

<P>380 nm Ultraviolet (UV) light emitting diodes (LEDs) were grown on patterned n-type GaN substrate (PNS). Wet etched self-assembled indium tin oxide (ITO) nano clusters serves as dry etching mask for converting the SiO2 layer grown on n-GaN template into SiO2 nano dots by inductively coupled plasma etching. In the pre-experiment, crystal quality and optical properties of n-GaN were greatly improved by applying PNS process. In this work, etch-pits density (EPD) method confirmed that PNS with SiO2 nano dots have superior crystalline properties. Thus Reference LED without PNS, 1-step PNS LEDs with SiO2 nano dots size were 200 nm, 250 nm, 300 nm and 300 nm 2-step PNS LED were fabricated. LEDs show almost the same operating voltage of about 3.4 V at an injection current of 50 mA. Light intensity was enhanced by ~2.1 times and 3.2 times for 300 nm 1-step and 300 nm 2-step PNS, respectively. FDTD simulation results show a similar tendency. As a result, PNS promotes epitaxial lateral overgrowth (ELOG) for defect reduction as well as act as a light scattering point.</P>

Possible Epithelial Ovarian Cancer Association with HPV18 or HPV33 Infection

Zhang, Pei-Pei,Zhou, Lei,Cao, Jia-Shi,Li, Yi-Ping,Zeng, Zhi,Sun, Ni,Shen, Li,Zhu, Hao-Yue,Ruan, Yang,Zha, Wen-Ting,Wang, Xin-Yu,Zhang, Ke-Qiang,Zhang, Ran Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.6

The present study was conducted to investigate the prevalence of HPV infection in epithelial ovarian cancer (EOC) in Hunan province. DNA samples were collected from paraffin embedded ovarian tissue from 322 patients with EOC, 99 with ovarian benign tumors and 199 normal persons. The polymerase chain reaction and direct sequencing were used to identify the HPV types in the samples. The relationship between the infection of human papillomavirus (HPV) and the epithelial ovarian carcinoma (EOC) was investigated combined with clinical data. The prevalence of HPV18 and HPV33 in EOC group and benign group was higher than in the normal group. HPV18 and HPV33 may play a role in the development of both EOC and ovarian benign tumor and may participate in the development of EOC with traditional risk factors, family history and abortion, possibly exerting synergistic effects.