Anti-metastasis Activity of Black Rice Anthocyanins Against Breast Cancer: Analyses Using an ErbB2 Positive Breast Cancer Cell Line and Tumoral Xenograft Model

Luo, Li-Ping,Han, Bin,Yu, Xiao-Ping,Chen, Xiang-Yan,Zhou, Jie,Chen, Wei,Zhu, Yan-Feng,Peng, Xiao-Li,Zou, Qiang,Li, Sui-Yan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Increasing evidence from animal, epidemiological and clinical investigations suggest that dietary anthocyanins have potential to prevent chronic diseases, including cancers. It is also noteworthy that human epidermal growth factor receptor 2 (ErbB2) protein overexpression or ErbB2 gene amplification has been included as an indicator for metastasis and higher risk of recurrence for breast cancer. Materials and Methods: The present experiments investigated the anti-metastasis effects of black rice anthocyanins (BRACs) on ErbB2 positive breast cancer cells in vivo and in vitro. Results: Oral administration of BRACs (150 mg/kg/day) reduced transplanted tumor growth, inhibited pulmonary metastasis, and decreased lung tumor nodules in BALB/c nude mice bearing ErbB2 positive breast cancer cell MDA-MB-453 xenografts. The capacity for migration, adhesion, motility and invasion was also inhibited by BRACs in MDA-MB-453 cells in a concentration dependent manner, accompanied by decreased activity of a transfer promoting factor, urokinase-type plasminogen activator (u-PA). Conclusions: Together, our results indicated that BRACs possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules.

Enhanced Room-Temperature Thermoelectric Performance of In-Doped ZnO:Al Thin Films through Prefabricated Layer Doping Method

Ping Fan,Zhuang-Hao Zheng,Jing-Ting Luo,Guang-Xing Liang,Dong-Ping Zhang 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.3

In this study, AZO thin films prepared by direct current reactive magnetron sputtering using a Zn-Al alloy target and In with varied content were doped through the prefabricated layer doping method in order to optimize their thermoelectric properties. The effects of In content on the room temperature microstructure and thermoelectric properties of the AZO thin films were investigated. It was found that the absolute value of the Seebeck coefficient of the thin films increases stably after In doping and reaches 153 μV·K−1 when the In content is 0.71%. Though the electrical conductivity of In-doped thin films is smaller than those of the un-doped films, the power factor of the thin films shows a significant increase after In doping with a maximum value of 2.22 × 10−4 W·m−1·K−2, which is several times that of the un-doped films.

Wavelength switchable flat-top all-fiber comb filter based on a double-loop Mach-Zehnder interferometer

Luo, Ai-Ping,Luo, Zhi-Chao,Xu, Wen-Cheng,Cui, Hu The Optical Society 2010 Optics express Vol.18 No.6

<P>A wavelength switchable all-fiber comb filter with flat-top spectral response based on a double-loop Mach-Zehnder (M-Z) interferometer is proposed and demonstrated. The proposed flat-top filter consists of a rotatable polarizer and a double-loop M-Z interferometer composed of two fiber couplers with a polarization controller (PC) in the first loop. In the theoretical analysis, when the second coupler of the M-Z interferometer is a non-3dB one, with proper settings of the polarization state of the input light and the PC, the wavelength switchable comb filter with flat-top passband can be obtained. Theoretical prediction was verified by experimental demonstration. The measured 1 dB bandwidth was 0.51 nm with a channel spacing of 0.98 nm, indicating that the flat-top passband of 1 dB bandwidth extends to about 50% of the comb spacing.</P>

A Single Chord Localization Algorithm for Wireless Sensor Networks

Yong-Luo Shen,Yuan Zhang,Yu-Ping Hu,Jun Zhang 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.1

This paper presents a novel range-free single chord localization algorithm with low energy consumption and high accuracy for wireless sensor networks. A mobile anchor node and a mobile reference node are employed to periodically broadcast beacon messages and reference messages, respectively. Each sensor node is equipped with a received signal strength indicator for comparing the signal strength. By using the edge function and the result of comparing signal strength, each sensor node only needs one chord on the communication circle for localization. Compared with previous approaches that require multiple chords, less beacon messages are required by the sensor nodes. In the proposed algorithm, the localization error mainly depends on the move distance interval. However, for the other approaches, large localization errors can be observed according to the angle between chords which may descend the localization accuracy dramatically. Analysis results indicate that the proposed algorithm is superior to other approaches in energy consumption and localization accuracy.

Trajectory Planning for Energy Minimization of Industry Robotic Manipulators Using the Lagrange Interpolation Method

Lu-Ping Luo,Hanyang Universit,Rui-Jun Yan,Quan Yuan,Jing Wu,신규식,한창수 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

We propose to use Lagrange interpolation method to express each joint trajectory function to realize trajectory planning for energy minimization of industrial robotic manipulators. We give the position constraints to the industrial robotic manipulators and the stability of the industrial robotic manipulators should be satisfied. In order to avoid Runge’s phenomenon of Lagrange interpolation method, we use the Chebyshev interpolation points for our approach. Through derivation, the angular velocity functions and angular acceleration functions can be obtained. Lagrange interpolation method can satisfy position constraints, and ensure the smoothness of joint angular positions, velocities, accelerations, and joint torques. By taking these functions into the Performance Index (PI) of energy minimization, as well as the direct iteration method used for optimization of energy consumption, we can obtain the optimal trajectory for industrial robotic manipulators.

MiRNA-15a Mediates Cell Cycle Arrest and Potentiates Apoptosis in Breast Cancer Cells by Targeting Synuclein-γ

Li, Ping,Xie, Xiao-Bing,Chen, Qian,Pang, Guo-Lian,Luo, Wan,Tu, Jian-Cheng,Zheng, Fang,Liu, Song-Mei,Han, Lu,Zhang, Jian-Kun,Luo, Xian-Yong,Zhou, Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: Recent studies have indicated that microRNA-15a (miR-15a) is dysregulated in breast cancer (BC). We aimed to evaluate the expression of miR-15a in BC tissues and corresponding para-carcinoma tissues. We also focused on effects of miR-15a on cellular behavior of MDA-MB-231 and expression of its target gene synuclein-${\gamma}$ (SNCG). Materials and Methods: The expression levels of miR-15a were analysed in BC formalin fixed paraffin embedded (FFPE) tissues by microarray and quantitative real-time PCR. CCK-8 assays, cell cycle and apoptosis assays were used to explore the potential functions of miR-15a in MDA-MB-231 human BC cells. A luciferase reporter assay confirmed direct targets. Results: Downregulation of miR-15a was detected in most primary BCs. Ectopic expression of miR-15a promoted proliferation and suppressed apoptosis in vivo. Further studies indicated that miR-15a may directly interact with the 3'-untranslated region (3'-UTR) of SNCG mRNA, downregulating its mRNA and protein expression levels. SNCG expression was negatively correlated with miR-15a expression. Conclusions: MiR-15a has a critical role in mediating cell cycle arrest and promoting cell apoptosis of BC, probably by directly targeting SNCG. Thus, it may be involved in development and progression of BC.

Phylogenetic Diversity and Antifungal Activity of Endophytic Fungi Associated with Tephrosia purpurea

( Ze Ping Luo ),( Hai Yan Lin ),( Wen Bing Ding ),( Hua Liang He ),( You Zhi Li ) 한국균학회 2015 Mycobiology Vol.43 No.4

Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.

The differences between copper sulfate and tribasic copper chloride on growth performance, redox status, deposition in tissues of pigs, and excretion in feces

Zheng, Ping,Pu, Bei,Yu, Bing,He, Jun,Yu, Jie,Mao, Xiangbing,Luo, Yuheng,Luo, Junqiu,Huang, Zhiqing,Luo, Chenggui,Wang, Shaohui,Chen, Daiwen Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.6

Objective: The objective of this experiment was to compare the effects of adding 130 mg/kg Cu from either copper sulfate (CS) or tribasic copper chloride (TBCC) on growth performance, mineral deposition in tissues, and the excretion in feces of pigs as well as changes in the mineral contents in tissues and feces when the supplemental Cu level was decreased from 130 mg/kg to 10 mg/kg. Methods: A total of 72 pigs ($32.6{\pm}1.2kg$) were randomly assigned to a CS diet or a TBCC diet with 6 pens per treatment. The trial lasted 102 d and included 3 phases (phase 1, 1 to 30 d; phase 2, 31 to 81 d; and phase 3, 82 to 102 d). The supplemental levels of Cu in the 2 treatments were 130 mg/kg in phase 1 and 2 and 10 mg/kg in phase 3. Results: The results showed that pigs fed the CS diet tended to have higher average daily gain than pigs fed the TBCC diet during d 1 to 81 (p<0.10). Compared with CS, TBCC increased the activities of aspartate transaminase (AST), ceruloplasmin, and superoxide dismutase in serum on d 30 (p<0.05). The TBCC decreased the Cu level in the liver on d 81 (p<0.05) and increased the Mn level in the liver on d 102 (p<0.05). The concentration of Cu in feces sharply decreased when the supplemental Cu level in diet changed from 130 mg/kg to 10 mg/kg in both diets (p<0.05). Conclusion: The result suggested that TBCC and CS had no significant difference on growth performance but TBCC had higher activities of AST and antioxidant enzymes and lower liver Cu than CS when pigs fed diets with 130 mg Cu /kg diet.

라그랑지 보간법을 이용한 로봇 매니퓰레이터의 토크 최소화를 위한 궤적계획

라로평(Luo, Lu-Ping),황순웅(Hwang, Soon-Woong),한창수(Hahn Chang-Su) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.4

본 논문에서는 로봇 매니퓰레이터의 토크 최소화를 위한 궤적계획을 위해 라그랑지 보간법을 이용한 Algorithm을 제안하였다. 이를 위해 로봇 매니퓰레이터의 위치에 대한 구속조건이 주어지고 안정성이 보장되어야 한다. 라그랑지 보간법 의 Runge’s 현상을 회피하기 위해 Chebyshev 보간점을 이용하여 시간 보간점을 설정하였고, 이에 대응하는 최적각도를 찾아 내어 라그랑지 보간법을 이용한 매끄러운 관절의 각도, 속도, 가속도 궤적을 얻을 수 있다. 로봇 매니퓰레이터의 토크 소비 최적화를 위한 성능지표를 선정하였으며, 계산된 궤적을 통해 이 성능지표가 최소값을 가지도록 반복 계산하는 과정을 거친 다. 이를 통해, 토크와 성능지표를 최소화 시키는 최적의 궤적을 얻을 수 있으며, 로봇 매니퓰레이터가 작업을 수행하기 위한 움직임의 안전성을 보장한다. This paper proposes an algorithm using Lagrange interpolation method to realize trajectory planning for torque minimization of robot manipulators. For the algorithm, position constraints of robot manipulators should be given and the stability of robot manipulators should be satisfied. In order to avoid Runge’s phenomenon, we set up time interpolation points using Chebyshev interpolation points. After that, we found suitable angle which corresponds to the points and then we got trajectories of joint's angle, velocity, acceleration using Lagrange interpolation method. We selected performance index for torque consumption optimization of robot manipulator. The method went through repetitive computation process to have minimum value of the performance index by calculated trajectory. Through the process, we could get optimized trajectory to minimize torque and performance index and guarantee safety of the motion for manipulator performance.

Trajectory for Robotic Manipulators with Torque Minimization by Using Hermit Interpolation Method

Lu-Ping Luo,Chao Yuan,Kyoo-Sik Shin,Chang-Soo Han 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

In this paper, we present a new method to get the trajectory of robotic manipulators with torque minimized. For the robotic manipulators for our research, just the position and velocity of the start and the final point are given. With given conditions and arbitrary chosen mutative points, we get the Hermite interpolation method used. Beside the start point and the final point, we will search for middle arbitrary chosen mutative points. Then the Hermite interpolation method is used to make sure the positions and velocities of the links satisfy the given conditions. With the change of position and velocity on the arbitrary chosen mutative points, with which taken into performance Index, the trajectory for torque minimization can be got.