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Hai-long Piao,Yuan-hu Xuan,Su Hyun Park,제병일,Soon Ju Park,Sung Han Park,김철민,Jin Huang,Guo Kui Wang,김민정,강상모,이인중,Taek-Ryoun Kwon,Yong Hwan Kim,Un-sang Yeo,이기환,손대영,한창덕 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.1
Calcineurin B-like protein-interacting protein kinases (CIPKs) are a group of typical Ser/Thr protein kinases that mediate calcium signals. Extensive studies using Arabi-dopsis plants have demonstrated that many calcium sig-natures that activate CIPKs originate from abiotic stresses. However, there are few reports on the functional demon-stration of CIPKs in other plants, especially in grasses. In this study, we used a loss-of-function mutation to charac-terize the function of the rice CIPK gene OsCIPK31. Expo-sure to high concentrations of NaCl or mannitol effected a rapid and transient enhancement of OsCIPK31 expression. These findings were observed only in the light. However, longer exposure to most stresses resulted in down-regulation of OsCIPK31 expression in both the presence and absence of light. To determine the physiological roles of OsCIPK31 in rice plants, the sensitivity of oscipk31::Ds, which is a transposon Ds insertion mutant, to abiotic stresses was examined during germination and seedling stages. oscipk31::Ds mutants exhibited hypersensitive phenotypes to ABA, salt, mannitol, and glucose. Com-pared with wild-type rice plants, mutants exhibited re-tarded germination and slow seedling growth. In addition, oscipk31::Ds seedlings exhibited enhanced expression of several stress-responsive genes after exposure to these abiotic stresses. However, the expression of ABA meta-bolic genes and the endogenous levels of ABA were not altered significantly in the oscipk31::Ds mutant. This study demonstrated that rice plants use OsCIPK31 to modulate responses to abiotic stresses during the seed germination and seedling stages and to modulate the expression of stress-responsive genes.
Piao, Hai-long,Xuan, Yuan-hu,Park, Su-Hyun,Je, Byoung-Il,Park, Soon-Ju,Park, Sung-Han,Kim, Chul-Min,Huang, Jin,Wang, Guo Kui,Kim, Min-Jung,Kang, Sang-Mo,Lee, In-Jung,Kwon, Taek-Ryoun,Kim, Yong-Hwan,Ye Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.1
Calcineurin B-like protein-interacting protein kinases (CIPKs) are a group of typical Ser/Thr protein kinases that mediate calcium signals. Extensive studies using Arabidopsis plants have demonstrated that many calcium signatures that activate CIPKs originate from abiotic stresses. However, there are few reports on the functional demonstration of CIPKs in other plants, especially in grasses. In this study, we used a loss-of-function mutation to characterize the function of the rice CIPK gene OsCIPK31. Exposure to high concentrations of NaCl or mannitol effected a rapid and transient enhancement of OsCIPK31 expression. These findings were observed only in the light. However, longer exposure to most stresses resulted in downregulation of OsCIPK31 expression in both the presence and absence of light. To determine the physiological roles of OsCIPK31 in rice plants, the sensitivity of oscipk31::Ds, which is a transposon Ds insertion mutant, to abiotic stresses was examined during germination and seedling stages. oscipk31::Ds mutants exhibited hypersensitive phenotypes to ABA, salt, mannitol, and glucose. Compared with wild-type rice plants, mutants exhibited retarded germination and slow seedling growth. In addition, oscipk31::Ds seedlings exhibited enhanced expression of several stress-responsive genes after exposure to these abiotic stresses. However, the expression of ABA metabolic genes and the endogenous levels of ABA were not altered significantly in the oscipk31::Ds mutant. This study demonstrated that rice plants use OsCIPK31 to modulate responses to abiotic stresses during the seed germination and seedling stages and to modulate the expression of stress-responsive genes.
Shen-Kang protects against tacrolimus-induced renal injury
Long Ye Zhang,Jian Jin,Kang Luo,Shang Guo Piao,Hai Lan Zheng,Ji Zhe Jin,임선우,최범순,양철우,Can Li 대한내과학회 2019 The Korean Journal of Internal Medicine Vol.34 No.5
Background/Aims: Evidence suggests that Shen-Kang (SK), a traditional Chinese herbal medicine, protects against various types of renal injury. In this study, we evaluated whether SK treatment confers renoprotection in a rat model of chronic tacrolimus (TAC) nephropathy. Methods: Rats were treated daily with TAC (1.5 mg/kg, subcutaneously) and SK (450 mg/kg, intravenously) for 4 weeks. The effects of SK on TAC-induced renal injury were assessed by measuring renal function, urine albumin excretion, histopathology, inflammatory cell infiltration, expression of profibrotic (transforming growth factor β1 [TGF-β1] and TGF-β inducible gene-h3 [βig-h3]) and proinflammatory cytokines, oxidative stress, and apoptotic cell death. Results: Administration of SK preserved glomerular integrity (fractional mesangial area and Wilms tumor 1-positive glomeruli), attenuated tubulointerstitial fibrosis, and reduced the number of ectodermal dysplasia 1-positive cells, and this was paralleled by improved urine albumin excretion and renal dysfunction. At the molecular level, SK treatment suppressed expression of TGF-β1/Smad2/3, βig-h3, and proinflammatory cytokines. Oxidative stress and apoptotic cell death were significantly decreased with SK treatment, and apoptosis-related genes were regulated toward cell survival (active caspase-3 and the B-cell lymphoma-2/ Bcl2-associated X [Bcl-2/Bax] ratio). Conclusions: SK protects against TAC-induced renal injury.
김철민,제병일,Hai Long Piao,Soon Ju Park,김민정,Sung Han Park,Jin Young Park,Su Hyun Park,Eun Kyeong Lee,Nam Soo Chon,Yong Jae Won,Gi Hwan Lee,Min Hee Nam,Doh Won Yun,Myung Chul Lee,Young Soon Cha,이곤호,은무영,한창덕 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.2
Many aspects of epigenetic phenomena have been elu-cidated via studies of transposable elements. An active transposable element frequently loses its ability to mobilize and goes into an inactive state during devel-opment. In this study, we describe the cyclic activity of a maize transposable element dissociation (Ds) in rice. In rice genome, Ds undergoes the spontaneous loss of mobility. However, an inactive state of Ds can be changed into an active state during tissue culture. The recovery of mobility accompanies not only changes in the methylation patterns of the terminal region of Ds, but also alteration in the steady state level of the acti-vator (Ac) mRNA that is expressed by a constitutive CaMV 35S promoter. Furthermore, the Ds-reactiva-tion process is not random, but stage-specific during plantlet regeneration. Our findings have expanded previous observations on Ac reactivation in the tissue culture of maize.
Park, Soon Ju,Piao, Hai Long,Xuan, Yuan-hu,Park, Sung Han,Je, Byoung Il,Kim, Chul Min,Lee, Eun Jin,Park, Soo Hyun,Ryu, ByeongChan,Lee, Kon Ho,Lee, Gi Hwan,Nam, Min Hee,Yeo, Un Sang,Lee, Myung Chul,Yun Korean Society for Molecular Biology 2006 Molecules and cells Vol.21 No.2
<P>Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.</P>
Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice
Kim, Chul-Min,Piao, Hai-Long,Park, Soon-Ju,Chon, Nam-Soo,Je, Byoung-Il,Bingyao Sun,Park, Sung-Han,Park, Jin-Young,Lee, Eun-Jin,Kim, Min-Jung,Chung, Woo-Sik,Lee, Kon-Ho,Lee, Young-Suk,Lee, Jeung-Joo,Wo Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
Rapid, large-scale generation of a Ds transposant population was achieved using a regeneration procedure involving tissue culture of seed-derived calli carrying Ac and inactive Ds elements. In the F_(2) progeny from genetic crosses between the same Ds and Ac starter lines, most of the crosses produced an independent germinal transposition frequency of 10-20%. Also, many Ds elements underwent immobilization even though Ac was expressed. By comparison, in a callus-derived regenerated population, over 70% of plants carried independent Ds insertions, indicating transposition early in callus formation. In the remaining population, the majority of plants carried only Ac. Most of the new Ds insertions were stably transmitted to a subsequent generation. An exceptionally high proportion of independent transposants in the regenerated population means that selection markers for transposed Ds and continual monitoring if Ac/Ds activities may not necessarily be required. By analyzing 1297 Ds-flanking DNA sequences, a genetic map of 1072 Ds insertion sites was developed. The map showed that Ds elements were transposed onto all of the rice chromosomes, with preference not only near donor sites (36%) but also on certain physically unlinked arms. Poulations from both genetic crossing and tissue culture showed the same distribution patterns of Ds insertion sites. The information of these mapped Ds insertion sites was deposited in GenBank. Among them, 55% of Ds elements were on predicted open-reading frame (ORF) regions. Thus, we propose an optimal strategy for the rapid generation of a large population of Ds transposants in rice.
Transposon <i>Ac/Ds</i> -induced chromosomal rearrangements at the rice <i>OsRLG5</i> locus
Xuan, Yuan Hu,Piao, Hai Long,Je, Byoung Il,Park, Soon Ju,Park, Su Hyun,Huang, Jin,Zhang, Jian Bo,Peterson, Thomas,Han, Chang-deok Oxford University Press 2011 Nucleic acids research Vol.39 No.22
<P>Previous studies have shown that pairs of closely-linked <I>Ac/Ds</I> transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line (<I>OsRLG5-161)</I> that contains two inversely-oriented <I>Ds</I> insertions in <I>OsRLG5</I> (<I>Oryza sativa</I> Receptor like kinase Gene 5). Among approximately 300 plants regenerated from <I>OsRLG5-161</I> heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented <I>Ds</I> elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted <I>Ds</I> elements in <I>OsRLG5-161</I>. The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual <I>Ds</I> termini. Together, these results demonstrate a greatly enlarged potential of the <I>Ac/Ds</I> system for plant chromosome engineering.</P>
Kim, Chul-Min,Je, Byoung-Il,Piao, Hai-Long,Park, Soon-Ju,Kim, Min-Jung,Park, Sung-Han,Park, Jin-Young,Park, Su-Hyun,Lee, Eun-Kyeong,Chon, Nam-Soo,Won, Yong-Jae,Lee, Gi-Hwan,Nam, Min-Hee,Yun, Doh-Won,L Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-
Many aspects of epigenetic phenomena have been elucidated via studies of transposable elements. An active transposable element frequently loses its ability to mobilize and goes into an inactive state during development. In this study, we describe the cyclic activity of a maize transposable element dissociation (Ds) in rice. in rice genome, Ds undergoes the spontaneous loss of mobility. However, and inactive state of Ds can be Changed into an active state during tissue culture. The recovery of mobility accompanies not only changes in the methylation patterns of the terminal region of Ds, but also alteration in the steady state level of the activator (Ac) mRNA that is expressed by a constitutive CaMV 35S promoter, Furthermore, the Ds-reactivation process is not random, but stage-specific during plantlet regeneration. Our findings have expanded previous observation on Ac reactivation in the tissue Culture of maize.
Byoung Il Je,Chul Min Kim,Hai Long Piao,Soon Ju Park,Sung Han Park,Soo Hyun Park,Jin Young Park,Eun Jin Lee,Hu Xuan Yuan,Jang Won Suk,Tae Il Kim,Sang-Nag Ahn,Yong Soo Hwang,Chang-Deok Han 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.2
Many strawberry cultivars in Korea and Japan are of mixed genetic pedigrees. We performed a random amplified polymorphic DNA (RAPD) study to distinguish among ten strawberry cultivars that have dominated the Korean and Japanese markets for the past 10 years. The cultivars examined were ‘Akihime’, ‘Tochinomine’, ‘Maeyhang’, ‘Sachinoka’, ‘Red Pearl’, ‘Tochiotome’, ‘Askarubi’, ‘Toyonoka’, ‘Nyoho’, and ‘Suhong’. In order to identify an optimal set of primers for generating polymorphic DNA products, we tested genomic DNA from petals or leaves with 163 10-mer primers. A set of five primers generated 15 polymorphic DNA fragments and each of the ten cultivars exhibited its own characteristic pattern of RAPD products. On average, seven different DNA bands were detected between any two given cultivars. A phylogenetic tree that was constructed on the basis of these 15 polymorphic DNA bands, reflects breeding pedigrees of some cultivars. Therefore, the data should assist breeders in rapid and accurate identification of strawberry cultivars.