알레르기 환자에서 혈청 IgE와 특이항원 양성율의 일치도

남해선,권준택,박준수 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

To evaluate usefulness of serum IgE to determine specific allergens, authors conducted priest IgE and MAST-CLA allergen-specific IgE assays in 78 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through July 2000. During the study period, inhalent and food Korean panels were applied in 57 (73.1%) and 21 outpatients (26.9%), respectively. Kappa index was calculated to evaluate the agreement of priest IgE with Total IgE and allergen-specific IgE by MAST-CLA. 1. The men (±SD) age was 13.2 (±14.1) year-old and male accounted for 62.8%. 2. The overall positive rate of priest IgE was 66.7% and mean (±SD) level of priest IgE was 271.2 (±265.6) IU/ml 3. Patients with more than one specific allergen and with elevated total IgE by MAST-CLA were 25 (32.1%) and 33 (42.3%), respectively. 4. Significant agreement between total IgE and allergen-specific IgE was shown, but strength of agreement was slight (Kappa = 0.186, P = 0.046), 5. While no significant agreement between priest IgE and total IgE was observer, the priest IgE agreed significantly with allergen-specific IgE (Kappa = 0.153, P=0.043) In conclusion, our data suggested that serum priest IgE is more likely associated with allergen-specific IgE than total IgE in MAST-CLA assay. Furthermore, it is recommended that Korean panel of MAST-CLA should contain more specific allergens.

유산균 발효유 음용의 인체 항산화 효과

남해선 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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이락촌폐흡충의 조직내 항원성부위의 미세구조적 관찰

남해선 순천향대학교 1994 논문집 Vol.17 No.4

An immunoelectron microscopic observation of various antigenic sites of the lung fluke, adult Paragonimus iloktsuenensis, was performed by applying the indirect immunoperoxidase technique to localize the selective antigenic sites. The fixative was mixture of 1% paraformaldehyde and 0.2% glutaraldehyde in 0.1M phosphate buffer, pH 7.4. LR-white resin(EMS, hard grade), the embedding media, was polymerized at -20℃ under the ultraviolet light(365nm long-wave length) for 48 hours. Nickel grids(EMS, 200 mesh) were coated with 2% collodion(in isoamyl-acetate) and 0.5% polyvinyl formvar. The antiserum produced from the rat infected with Paragonimus iloktsuenensis(10 weeks after infection) was used as a primary antibody. The secondary antibody was gold conjugated goat anti-rat IgG(particle size=10nm, Sigma). The intestinal epithelial cells containing numerous antigenic granules which seem to be secreted into the intestinal lumen. The secretory granules and luminal contents were predominantly labeled by gold particles. The excretory bladder was also proved to be a highly antigenic organ, since its lamellated structures and contents were labeled by numerous gold particles. The antigenicities of secretory materials of the vitelline glands, testes, and ovaries were moderately recognizible, too. But the tegument was very weakly labeled by gold particles. The above results suggests that the secretory substances from the intestinal epithelial cells and excretory contents from excretory bladder in the adult Paragonimus iloktsuenensis are the most potent antigenic stimulants to the host immune system.

Escherichia coli 패혈증 환자에 합병된 대칭적 하지 말단 괴사증 1예

남해성,유진홍,권순석,민준기,조현선,박민경,심병주,남유정,이지인,김진수,길욱현,조근종,신완식 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

We have encountered a rare case of symmetrical peripheral gangrene complicating Escherichia coli sepsis in a 47-years-old male. He was successfully treated with antibiotics, anticoagulants, and vasodilator. To our knowledge, this is the first report on symmetrical peripheral gangrene complicating E. coli sepsis in Korea.

대호만 참붕어의 흡충류 피낭유충 감염상

남해선 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

This study was done in December 1998, for research on the actual condition of the infection status of trematode metacercariae in Pseudorasbora parva caught from Daehoman(pond) located in Sosan-Si, Chungnam. Total of 73 Pseudorasbora parva were examined under stereomicroscope after artificial digestion with pepsin-HCl solution. Three species of metacercariae were found, and each numbers of collected total metacercariae were 157 of Clonorchis sinensis, 124 of Methorchis orientalis and 39,092 of Cyathocotyle orientalis. The average numbers of detected metacercariae per each Pseudorasbora parva were Clonorchis sinensis 2.2, Methorchis orientalis 1.7 and Cyathocotyle orientalis 535.5.

천안시 북면 연춘리 수계의 메타고니무스 흡충에 관한 보고

남해선 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

The Metagonimus species cercaria and metacercaria were seperated each from snail of Semisulcospira sp. and fresh water fish of Zacco platypus in Byongchun stream of Buk Myeon, Chunan City of Korea, and were observed with scanning electron microscope for cercaria and light microscope for metacercaria. The cercaria was divided into two parts, trigonal shaped body and long tapering ended tail. The body part has oral sucker on anterior tip. Several paired ciliated sensory papillae distributed on body surface and around oral sucker. The round shaped metacercaria was surrounded with transparent membrane. The excretory bladder was seen with darkish brown color. This Metagonimus sp. was surely Metagonimus Miyata type based on its morphology of cercaria, local distribution and specific fish host of Zacco platypus. The result of this study suggest that Chunan area is one of the endemic foci of the Metagonimiasis.

베개내 세균성 내독소와 집먼지진드기 항원 농도의 비교

남해선 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

베개에는 일반적으로 많은 양의 실내 알레르겐과 세균성 내독소가 있는 것으로 알려져 있으며, 이는 집먼지진드기 항원에 감작되어 있는 사람에게는 매우 중요시해야 하는 요소 중의 하나로 되어 있다. 메밀베개는 한국에서는 일반적으로 사용되고 있는 베개이다. 이번 실험을 통하여 메밀베개와 일반 스폰지 베개 내의 집먼지진드기 항원의 양과 내독소의 양이 사용함에 따라 어떻게 변하는지에 대하여 규명해 보고자 천안시에 거주하는 13가구에서 새로 구입하여 지급한 두가지 타입의 베개를 3개월간 같은 침대에서 사용토록한 후 사용전후의 내독소와 집먼지진드기의 양을 서로 비교하였다. 새 베개에서는 두 가지 타입 모두에서 집먼지진드기(Der f 1 및 Der p 1) 항원이 측정되지 않았다. 3개월간 사용한 후에 측정한 Der f 1 의 농도는 메밀베개와 스폰지베개에서 평균이 각각 1,16 g/g (0.02-8.13)와 1.08 g/g (0.19-1.68)로 95% 신뢰구간에서 유의한 차이가 없었다. 그러나 새로 구입한 메밀베개와 스폰지베개에서 측정한 내독소의 양은평균이 각각 60.950 EU/g (30,270-122,700) 와 4,887 EU/g (2,570-9,311) 로 95%의 신뢰구간에서 유의한 차이가 있었다. 3개월간 사용한 후에 측정된 Der f 1 의 양은 메밀베개와 스폰지 베개에서 평균이 각각1.16 g/g (0.02-8.13) 과 1.08 g/g (0.19-1.68) 로 95% 신뢰도 구간에서 유의한 차이가 없었다. 이러한 결과를 통하여 메밀베개는 매우 많은 양의 내독소를 함유하고 있어서 심한 알레르기성 천식의 유발원인이 될 수 있음을 확인하였다.

학교급식 보조 유무에 따른 아동의 사회인구학적 특성과 식생활 태도 비교

남혜원,우인애,변진원 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.1

This study was carried out to compare dietary attitudes between the children with school lunch fee provided by parents and by the government. The subjects of this study was consisted of 460 elementary school students in the 6th grade(407 lunch-affordable children, 53 lunch-unaffordable children). General characteristics was significantly different in two groups. In the free group family size(P<0.001), percentage of living with parents (P<0.001) and education level of parents(father: P<0.05, mother: (P<0.01)) was lower than in the charged group, but the dependence on mother's income(P<0.001) and financial support of government (P<0.001) was higher. There was no significant difference in anthropometric measurements by lunch fee. The preference to cooking method was significantly different (P<0.01), the charged group liked grill except frying and roasting but the free group liked seasoning and salting. When comparing eating habits, general eating behavior(P<0.05) was significantly lower in the free children and especially regularity of breakfast (P<0.05) and daily intake of raw vegetables (P<0.05), fruits(P<0.01). There was no significant difference in the total score of nutrition knowledge between the children of the two groups.

유자차 부산물을 이용한 유자 과편의 최적화 연구

남혜원,현영희,변진원 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.6

This study was performed to develop a standardized recipe for yuza pyun made of citron juice that is by-product of citron tea, to increase the utilization of citron. The effects of mungbean starch(15%, 18%) and dilution ratio of yuza extract(100%, 75%, 50%) on the quality characteristics were evaluated. The quality characteristics of the samples were estimated in terms of sweetness, color difference, texture profile analysis and sensory evaluation. Sweetness of the 18% mungbean groups were higher than 15% groups(p<0.001). The values of Hunter color system indicated that higher content of yuza extract tended to increase yellowness significantly(p<0.001). Texture profiles of hardness, adhesiveness, springiness, cohesiveness, gumminess of yuzapyun showed no significant differences among samples. For sensory evaluation, color and sourness were decreased as yuza extract was diluted, whereas elasticity and sweetness were increased. Yuza pyun containing 18% of mungbean starch with 50% diluted extract was the most preferred in overall acceptability. According to multiple regression analysis, color, sweetness, elasticity and sourness(in order) were important control factors in overall acceptability.

알레르기성 소인이 없는 정상인에서 특이 알레르겐항체 양성율

남해선,안현철,박준수,황규윤,박춘식 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

This study was designed to evaluate prevalence of allergen-specific antibodies in healthy adults. During Mar. ~ Aug. 2001, 1,925 subjects visited for medical check-up, were inquired to fill out a standardized questionnaire. Among them, 200 subjects who had no current and past allergic disorders were randomly selected for rhe current study and examined for allergen-specific antibodies using Korean inhalant panel of MAST-CLA allergen-specific IgE assay. Among them, 170 persons (85.0%) did not have any positive allergen-specific antibody. Each positive rate of 35 allergen specific antibodies was usually less than 3%, except Cockroach Mix (3.5%), Dermatophagoides farinae(8.5%), Dermatophagoides pteronyssinus(6.0%). Among 30 positive subjects, 19(63.3%) showed one or two allergen-specific antibodies. Although atopic subjects in non-allergic population were not common, few of them showed allergen-specific antibodies in Korean inhalant panel of MAST-CLA. Our results suggested that the standardized questionnaire and MAST-CLA could be an useful tool of a screening test for allergy, showing high specificity and sensitivity.