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Effect of Nutrition Counseling Program on Weight Control in Obese University Students
( Bo Ryeong Kim ),( Seung Yeon Seo ),( Na Gyeong Oh ),( Jung-sook Seo ) 한국임상영양학회 2017 Clinical Nutrition Research Vol.6 No.1
This study was conducted to investigate the effect of nutrition counseling program and related factors on weight control for obese university students. Subjects were 24 students with a body mass index (BMI) of 25 or above. The program was conducted from September 16th to November 18th, 2015. Change of body composition, blood index and nutrient intake were observed in subjects before and after the program. The average age of the subjects was 23.2 years old and the percentage of male and female was 66.7% and 33.0%, respectively. There were tendencies of decrease in weight, amount of body fat, BMI, and body fat percentage. The blood test showed that values of all biochemical parameters were in the normal range before and after the program. When the change of the nutrient intake was examined and compared with the Dietary Reference Intakes for Koreans (KDRIs), there was a tendency of decreased intake in most of the nutrients including protein. However, the nutrient quality index showed increasing tendency, which implies that the intake of micronutrients was getting balances simultaneously with the decrease of calorie intake.
Yeon Sun Kim,Gyeong Ryeong Kim,박미라,양승철,So Hee Park,원지은,이주희,Ha Eun Shin,Haengseok Song,김혜련 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.1
BACKROUND: CRISPR/Cpf1 is a class II, type V RNA-guided endonuclease that is distinct from the type II CRISPR/Cas9 nuclease, widely used for genome editing. Cpf1 is a smaller and simpler endonuclease than Cas9, overcoming some limitations of the CRISPR/Cas9 system. The applications of CRISPR to rodent embryos for the production of knock-out (KO) mice have been achieved mainly by microinjection, which requires heavily-equipped instruments with skillful hands. Here, we evaluated the genome editing efficiency between Cpf1/mRNA and Cpf1/ribonuclear protein (RNP) in mouse embryos, and established an easy, fast, and technically less demanding method to produce KO mice using electroporation of the Cfp1/RNP system. METHODS: The efficiency of electroporation-based delivery of AsCpf1/mRNA and AsCpf1/RNP to target exon 3 of leukemia inhibitory factor (Lif) into mouse zygotes was evaluated. Embryos that developed to the two-cell stage after zygote electroporation were transferred into the oviducts of surrogate mothers to produce AsCpf1-mediated LIF KO mice. The genome editing efficiency of blastocysts and pups was tested using the T7E1 assay and/or DNA sequencing. Congenital abnormalities and reproductive phenotypes in LIF KO mice produced by electroporation with AsCpf1/RNP were examined. RESULTS: Survival and two-cell development of electroporated zygotes were comparable between the AsCpf1/mRNA and AsCpf1/RNP groups, whereas genome editing efficiency was relatively higher in the AsCpf1/RNP group (13.3% vs 18.1% at blastocyst and 33.3% vs 45.5% at offspring), respectively. Two mouse lines with a frameshift mutation in exon 3 of the Lif gene were established from the AsCpf1/RNP group. All congenital abnormalities of LIF KO mice produced by AsCpf1/RNP electroporation were observed. AsCpf1-mediated LIF KO mice showed postnatal growth retardation and implantation failure, both of which are major phenotypes of LIF KO mice generated by conventional gene targeting. CONCLUSION: Electroporation of AsCpf1/RNP at the zygote stage is an efficient genome editing method to produce KO mice.
김봉환,한선희,신지은,김혜령,강보경,박정현,Kim, Bong-Hwan,Han, Sun-Hee,Shin, Ji-Eun,Kim, Hye-Ryeong,Kang, Bo-Gyeong,Park, Jeong-Hyeon 한국임상보건과학회 2018 한국임상보건과학회지 Vol.6 No.1
Purpose. The purpose of this study was to investigate the effect of lens color of sunglasses on stereoacuity by experiment. Methods. We conducted a stereotaxic test using a RANDOM DOT with a polarizing lens at an examination distance of 40 cm in 100 men and women aged 20 to 60 years without any specific ocular disease. Then, seven kinds of PVC color film such as yellow, black, brown, red, purple, blue and green were put on the polarizing lens and the stereoscopic examination was carried out again. Results. When the lens color of the sunglasses is yellow, black, and brown, it affects less stereoscopic effect. However, red, purple, blue, and green have much influence on stereoscopic effect. Especially, the red and purple colors affected the stereoscopic vision. In blue and green, the effect of stereoscopic vision was different according to the people. Conclusions. Depending on the lens color of the sunglasses, stereoscopic vision is affected, which can cause problems in binocular vision. Therefore, when choosing sunglass lens color, many buyers need to pay attention.