EFFECTS OF CARBON NANOTUBES ON RAT LIVER AND BRAIN

FU-DE WANG,CHAN JIN,LING-LING CAO,HAO LIANG,CHUN-WANG MA 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7

Carbon nanotubes (CNTs) have been widely applied in various ¯elds due to excellent physicaland chemical properties. As production and applications of nanotubes expand, public concernabout their potential risks to human health has also risen. In the present study, the e®ects ofCNTs on rat liver and brain by single intratracheal instillation were detected. CNTs [eithersingle-walled carbon nanotubes (SWCNTs) or multi-walled carbon nanotubes (MWCNTs)] couldbe seen in the lung and liver indicating the transfer of CNTs by blood stream. CNTs could induceoxidative stress in liver with elevated Malondialdehyde (MDA) level and degressive GSH level,superoxide dismutase (SOD) and CAT activity. To brain, maybe due to the blood brain barrierand the increased SOD and CAT activity, serious oxidative stress of brain did not occur.

B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway‑associated markers

Ji-Shui Wang,Fang Ruan,Li-Zhu Guo,Feng-Ge Wang,Fu-Ling Wang,Hong-Min An 한국유전학회 2021 Genes & Genomics Vol.43 No.5

Background Aberrant expression of beta-1,3-N-acetylglucosaminyltransferase-3 (B3GNT3) has been frequently clarifed in various cancers, however, its role in endometrial cancer (EC) has not been assessed in detail. Purpose This study aimed to investigate the biological role of B3GNT3 in EC and simply explored the detailed mechanism. Methods The EC RNA-Seq dataset from TCGA database was applied to evaluate the expression of B3GNT3 and assess its role on prognostic value. HEC-1-A and KLE cell lines of EC were used to perform loss- and gain-of-function B3GNT3 assays respectively. Quantitative real-time PCR (qRT-PCR) and western blot were used to measure the mRNA and protein levels of indicated molecules respectively. Cell counting kit-8, clone formation tests, and Transwell assay served to determine the changes of proliferative, invasive and migratory abilities of EC cells after altering the expression of B3GNT3. Results B3GNT3 was found to be highly expressed in EC tissues compared to normal tissues according to the online public databases, which confrmed by the following qRT-PCR in 3 EC cell lines. Besides, high B3GNT3 expression presented a worse overall survival in EC patients as compared with low B3GNT3 expression group. Furthermore, functional experiments in vitro indicated that B3GNT3 could facilitate the cell growth, invasion and migration. Moreover, we found that downregulation of B3GNT3 signifcantly reduced the expression level of GTP-RhoA and GTP-RAC1, whereas upregulation of B3GNT3 presented the opposite results. Conclusion The results of current study demonstrate that B3GNT3 acts as an oncogene that promotes EC cells growth, invasion and migration possibly through regulating the RhoA/RAC1 signaling pathway-related markers, suggesting that B3GNT3 may be a candidate biomarker for EC therapeutic intervention.

Carboxymethyl Flavonoids and A Monoterpene Glucoside from Selaginella moellendorffii

Hong-Sheng Wang,Ling Sun,Yue-Hu Wang,Ya-Na Shi,Gui-Hua Tang,Fu-Wei Zhao,Hong-Mei Niu,Chun-Lin Long,Ling Li 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.8

A new dihydroflavone, 5-carboxymethyl-7,4'-dihydroxyflavonone (1), and its glucoside 5-carboxymethyl-7,4'-dihydroxyflavonone-7-O-β-D-glucopyranoside (2), and one new monoterpene glucoside, (4Z,6E)-2,7-dimethyl-8-hydroxyocta-4,6-dienoic acid 8-O-β-D-glucopyranoside (3), were isolated from the whole plants of Selaginella moellendorffii. Their structures were determined by spectroscopic methods and chemical transformation. Compound 2 was evaluated for the ability to enhance glucose consumption in normal and insulin-resistant L6 muscle cells induced by high concentrations of insulin and glucose. Glucose consumption in insulin-resistant cells (but not in normal cells) was increased 15.2 ± 3.3% (p < 0.01) by compound 2 at a concentration of 0.1 μM in the presence of insulin (1 nM).

Drainage Alone or Combined with Anti-tumor Therapy for Treatment of Obstructive Jaundice Caused by Recurrence and Metastasis after Primary Tumor Resection

Xu, Chuan,Huang, Xin-En,Wang, Shu-Xiang,Lv, Peng-Hua,Sun, Ling,Wang, Fu-An,Wang, Li-Fu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Aim: To compare drainage alone or combined with anti-tumor therapy for treatment of obstructive jaundice caused by recurrence and metastasis after primary tumor resection. Materials and Methods: We collect 42 patients with obstructive jaundice caused by recurrence and metastasis after tumor resection from January 2008 - August 2012, for which percutaneous transhepatic catheter drainage (pTCD)/percutaneous transhepatic biliary stenting (pTBS) were performed. In 25 patients drainage was combined with anti-tumor treatment, antineoplastic therapy including intra/postprodure local treatment and postoperative systemic chemotherapy, the other 17 undergoing drainage only. We assessed the two kinds of treatment with regard to patient prognosis. Results: Both treatments demonstrated good effects in reducing bilirubin levels in the short term and promoting liver function. The time to reobstruction was 125 days in the combined group and 89 days in the drainage only group; the mean survival times were 185 and 128 days, the differences being significant. Conclusions: Interventional drainage in the treatment of the obstructive jaundice caused by recurrence and metastasis after tumor resection can decrease bilirubin level quickly in a short term and promote the liver function recovery. Combined treatment prolongs the survival time and period before reobstruction as compared to drainage only.

Safety and Efficacy of Sequential Transcatheter Arterial Chemoembolization and Portal Vein Embolization prior to Major Hepatectomy for Patients with HCC

Xu, Chuan,Lv, Peng-Hua,Huang, Xin-En,Wang, Shu-Xiang,Sun, Ling,Wang, Fu-An,Wang, Li-Fu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: To evaluate the safety and efficacy of sequential transcatheter arterial chemoembolization (TACE) and portal vein embolization (PVE) before major hepatectomy for patients with hepatocellur carcinoma (HCC). Methods: In this retrospective case-control study, data were collected from patients who underwent sequential TACE and PVE prior to major hemihepactectomy. Liver volumes were measured by computed tomography volumetry before TACE, and preoperation to assess degree of future remnant liver (FRL) hypertrophy and to check whether intro- or extrohepatic metastasis existed. Liver function was monitored by biochemistry after TACE, prior to and after major hepatectomy. Results: Mean average FRL volume increased 32.3-71.4% (mean 55.4%) compared with preoperative FRL volume. After TACE, liver enzymes were elevated, but returned to normal in four weeks. During PVE and resection, no patient had intro- or extrohepatic metastasis. Conclusion: Sequential TACE and PVE is an effective method to improve resection opportunity, expand the scope of surgical resection, and greatly reduce postoperative intra- and extrahepatic metastasis.

Role of Blood Pressure Management in Stroke Prevention: A Systematic Review and Network Meta-Analysis of 93 Randomized Controlled Trials

Xiao-Ling Zhong,Yi Dong,Wei Xu,Yu-Yuan Huang,Hui-Fu Wang,Tian-Song Zhang,Li Sun,Lan Tan,Qiang Dong,Jin-Tai Yu 대한뇌졸중학회 2021 Journal of stroke Vol.23 No.1

Background and Purpose: The present study aimed to compare the efficacy and tolerability of different blood pressure (BP)-lowering strategies. Methods: Randomized controlled trials that compared various antihypertensive treatments and stroke outcomes were included. Eligible trials were categorized into three scenarios: single or combination antihypertensive agents against placebos; single or combination agents against other agents; and different BP-lowering targets. The primary efficacy outcome was the risk reduction pertaining to strokes. The tolerability outcome was the withdrawal of drugs, owing to drug-related side effects (PROSPERO registration number CRD42018118454 [20/12/2018]). Results: The present study included 93 trials (average follow-up duration, 3.3 years). In the pairwise analysis, angiotensin-converting enzyme inhibitors (ACEis) and beta-blockers (BBs) were inferior to calcium channel blockers (CCBs) (odds ratio [OR], 1.123; 95% confidence interval [CI], 1.008 to 1.252) (OR, 1.261; 95% CI, 1.116 to 1.425) for stroke prevention, BB was inferior to angiotensin II receptor blockers (ARB) (OR, 1.361; 95% CI, 1.142 to 1.622), and diuretics were superior to ACEi (OR, 0.871; 95% CI, 0.771 to 0.984). The combination of ACEi+CCB was superior to ACEi+diuretic (OR, 0.892; 95% CI, 0.823 to 0.966). The network meta-analysis confirmed that diuretics were superior to BB (OR, 1.34; 95% CI, 1.11 to 1.58), ACEi+diuretic (OR, 1.47; 95% CI, 1.02 to 2.08), BB+CCB (OR, 2.05; 95% CI, 1.05 to 3.79), and renin inhibitors (OR, 1.87; 95% CI, 1.25 to 2.75) for stroke prevention. Regarding the tolerability profile, the pairwise analysis revealed that ACEi was inferior to CCB and less tolerable, compared to the other treatments. Conclusions: Monotherapy using diuretics, CCB, or ARB, and their combinations could be employed as first-line treatments for stroke prevention in terms of efficacy and tolerability.

1.54 μm photoluminescence emission at room-temperature of erbium-implanted lithium niobate crystal

Gang Fu,Shi Ling Li,Xue Lin Wang,Feng Chen,Ke Ming Wang 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.-

Erbium doped materials are of great interest in integrated optoelectronic technology, due to their Er³+ intra-4f emission at 1.54 μm, a standard telecommunication wavelength. Lithium niobate crystals (LN) are of great importance for fabrication of integrated optical devices, due to their excellent properties such as high electro-optical coefficient, low propagation loss, and high Curie temperature. Lithium niobate crystal has been implanted with 500 keV Er ions at a fluence of 3.0×10 15 ions/㎝² with the aim of optically doping the material in the near surface region. In order to recrystallize the amorphized implanted region the sample was annealed at 500℃ for 90 min in oxygen atmosphere. Photoluminescence (PL) and Rutherford backscattering spectrometry studies were performed on the as-implanted sample before and after annealing. 1.54 μm room-temperature photoluminescence emission was observed in the annealed sample. The relationship between annealing temperature and photoluminescence intensity is discussed.

Effects of Multiple-target Anti-microRNA Antisense Oligodeoxyribonucleotides on Proliferation and Migration of Gastric Cancer Cells

Xu, Ling,Dai, Wei-Qi,Xu, Xuan-Fu,Wang, Fan,He, Lei,Guo, Chuan-Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Backgrounds: To investigate the inhibiting effects of multi-target anti-microRNA antisense oligonucleotide (MTg-AMOs) on proliferation and migration of human gastric cancer cells. Methods: Single anti-microRNA antisense oligonucleotides (AMOs) and MTg-AMOs for miR-221, 21, and 106a were designed and transfected into SGC7901, a gastric cancer cell line, to target the activity of these miRNAs. Their expression was analyzed using stem-loop RT-PCR and effects of MTg-AMOs on human gastric cancer cells were determined using the following two assay methods: CCK8 for cell proliferation and transwells for migration. Results: In the CCK-8 cell proliferation assay, $0.6{\mu}mol/L$ was selected as the preferred concentration of MTg-AMOs and incubation time was 72 hours. Under these experimental conditions, MTg-AMOs demonstrated better suppression of the expression of miR-221, miR-106a, miR-21 in gastric cancer cells than that of single AMOs (P = 0.014, 0.024; 0.038, respectively). Migration activity was also clearly decreased as compared to those in randomized and blank control groups ($28{\pm}4$ Vs $54{\pm}3$, P <0.01; $28{\pm}4$ Vs $59{\pm}4$, P < 0.01). Conclusions: MTg-AMOs can specifically inhibit the expression of multiple miRNAs, and effectively antagonize proliferation and migration of gastric cancer cells promoted by oncomirs.

Imaging x-ray crystal spectrometer on EAST

Shi, Yuejiang,Wang, Fudi,Wan, Baonian,Bitter, Manfred,Lee, Sanggon,Bak, Jungyo,Hill, Kennith,Fu, Jia,Li, Yingying,Zhang, Wei,Ti, Ang,Ling, Bili Published jointly by The Institute of Physics and 2010 Plasma physics and controlled fusion Vol.52 No.8

<P>A high-resolution imaging x-ray crystal spectrometer is described for implementation on the EAST tokamak to provide spatially and temporally resolved data on the ion temperature, electron temperature and poloidal plasma rotation. These data are derived from observations of the satellite spectra of helium-like argon, Ar XVII, which is the dominant charge state for electron temperatures in the range from 0.4 to 3.0 keV and which is accessible to EAST. Employing a novel design, which is based on the imaging properties of spherically bent crystals, the spectrometers will provide spectrally and spatially resolved images of the plasma for all experimental conditions, which include ohmically heated discharges as well as plasmas with rf and neutral-beam heating. The experimental setup and initial experimental results are presented.</P>

Barrier Optical Waveguide Fabrication in LiNbO3 Crystal by 4.5-MeV LithiumIon Implantation with Low Dose

Xue-Lin Wang,Ding-Yu Shen,Gang Fu,Hong-Ji Ma,Ke-Ming Wang,Rui Nie,Shi-Ling Li 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.1

A barrier planar waveguide was fabricated in z-cut LiNbdO3 crystals by 4.5-MeV lithium ion implantation at a dose of 3 £ 1014 ions/cm2 at room temperature. Dark modes were observed by the prism-coupling method with wavelengths of both 633 nm and 1539 nm. The refractive index pro¯les were reconstructed by using the re°ectivity calculation method. There were about 1.1 % and 0.7 % decreases at the optical barriers of the ordinary and extraordinary refractive index at the wavelength at 633 nm, and the positions of the optical barriers were close to those of the damage peaks calculated by the TRIM098 (Transport of Ions in Matter) code. It is found that the refractive index change may be partly due to the damage induced by nuclear collision.