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Molecular organization of the B mating type locus of a Lentinula edodes monokaryon strain SUP2
Bao Da-Peng Chen Ming-Jie,Song Wen-Hua,Song Chun-yan,Zhang Mei-Yan,Chen Xiang,Lin Nan 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Lentinula edodes is an important cultivated mushroom in China. The development of Lentinula edodes production promotes more studies on it. In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the whole genome sequencing of the protoplast monokaryon of Lentinula edodes strain 135, to amplify STE3-like pheromone receptor gene and its flanking conserved genes in the protoplast monokaryon strain SUP2 derived from Lentinula edodes strain Suxiang and 33655bp DNA sequence was obtained. By BlastX search, seven putative genes were identified, and three of them are pheromone receptor encoded genes. Furthermore, near to two pheromone receptor genes, four genes encoding proteins with conserved motifs of pheromone precursors were found. This study firstly reveals the molecular organization of the B mating type locus of Lentinula edodes.
Sun, Jian-Da,Chen, Chuang-Zhen,Chen, Jian-Zhou,Li, Dong-Sheng,Chen, Zhi-Jian,Zhou, Ming-Zhen,Li, De-Rui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Treatment responses of $N_0$ stage nasopharyngeal carcinoma were firstly analyzed comprehensively to evaluate long term outcomes of patients and identify prognostic factors. A total of 610 patients with $N_0$ NPC, undergoing definitive radiotherapy to their primary lesion and prophylactic radiation to upper neck, were reviewed retrospectively. Concomitant chemotherapy was administrated to 65 out of the 610. Survival rates of the patients were calculated using the Kaplan-Meier method and compared by log-rank test. Prognostic factors were identified by the Cox regression model. The study revealed the 5-year and 10-year overall, disease-free, disease-specific, local failure-free, regional failure-free, locoregional failure-free and distant metastasis-free survival rates to be 78.7% and 66.8%, 68.8% and 55.8%, 79.9% and 70.4%, 81.2% and 72.5%, 95.8% and 91.8%, 78.3% and 68.5%, 88.5% and 85.5%, respectively. There were 192 patients experiencing failure (31.5%) after radiotherapy or chemoradiotherapy. Of these, local recurrence, regional relapse and distant metastases as the first event of failure occurred in 100 (100/610, 16.4%), 15(15/610, 2.5%) and 52 (52/610, 8.5%), respectively. Multivariate analysis showed that T stage was the only independent prognostic factor for patients with $N_0$ NPC (P=0.000). Late T stage (P=0.000), male (P=0.039) and anemia (P=0.007) were independently unfavorable factors predicting disease-free survival. After treatment, satisfactory outcome wasgenerally achieved in patients with $N_0$ NPC. Local recurrence represented the predominant mode of treatment failure, while T stage was the only independent prognostic factor for overall survival. Late T stage, male gender, and anemia independently predicted lower possibility of the disease-free survival.
Development of High Spectral Resolution Lidar System for Measuring Aerosol and Cloud
Ming Zhao,Chen-Bo Xie,Zhiqing Zhong,Bang-Xin Wang,Zhenzhu Wang,Pang-Da Dai,Zhen Shang,Min Tan,Dong Liu,Yingjian Wang 한국광학회 2015 Current Optics and Photonics Vol.19 No.6
A high spectral resolution lidar (HSRL) system based on injection-seeded Nd:YAG laser and iodineabsorption filter has been developed for the quantitative measurement of aerosol and cloud. The laserfrequency is stabilized at 80 MHz by a frequency locking system and the absorption line of iodine cellis selected at the 1111 line with 2 GHz width. The observations show that the HSRL can provide verticalprofiles of particle extinction coefficient, backscattering coefficient and lidar ratio for cloud and aerosolup to 12 km altitude, simultaneously. For the measured cases, the lidar ratios are 10~20 sr for cloud,28~37 sr for dust, and 58~70 sr for urban pollution aerosol. It reveals the potential of HSRL to distinguishthe type of aerosol and cloud. Time series measurements are given and demonstrate that the HSRL hasability to continuously observe the aerosol and cloud for day and night
Controlling the Thickness of Thermally Expanded Films of Graphene Oxide
Chen, Xianjue,Li, Wei,Luo, Da,Huang, Ming,Wu, Xiaozhong,Huang, Yuan,Lee, Sun Hwa,Chen, Xiong,Ruoff, Rodney S. American Chemical Society 2017 ACS NANO Vol.11 No.1
<P>'Paper-like' film material made from stacked and overlapping graphene oxide sheets can be exfoliated (expanded) through rapid heating, and this has until now been done with no control of the final geometry of the expanded graphene oxide material, i.e., the expansion has been physically unconstrained. (As a consequence of the heating and exfoliation, the graphene oxide is 'reduced', i.e., the graphene oxide platelets are deoxygenated to a degree.) We have used a confined space to constrain the expanding films to a controllable and uniform thickness. By changing the gap above the film, the final thickness of expanded films prepared from, e.g., a 10 mu m-thick graphene oxide film, could be controlled to values such as 20, 30, 50, or 100 mu m. When the expansion of the films was unconstrained, the final film was broken into pieces or had many cracks. In contrast, when the expansion was constrained, it never cracked or broke. Hot pressing the expanded reduced graphene oxide films at 1000 degrees C yielded a highly compact structure and promoted graphitization. Such thickness-controlled expansion of graphene oxide films up to tens or hundreds of times the original film thickness was used to emboss patterns on the films to produce areas with different thicknesses that remain connected 'in plane'. In another set of experiments, we treated the original graphene oxide film with NaOH before its controlled expansion resulted in a different structure featuring uniformly distributed pores and interconnected layers as well as simultaneous activation of the carbon.</P>
Xanthones from Garcinia paucinervis with in vitro anti-proliferative activity against HL-60 cells
Da-Hong Li,Chen-Xi Li,Cui-Cui Jia,Ya-Ting Sun,Chun-Mei Xue,Jiao Bai,Hui-Ming Hua,Xiao-Qiu Liu,Zhan-Lin Li 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.2
Three new xanthones, paucinervins H–J (1–3), as well as eleven known compounds (4–14), were isolated from the leaves of Garcinia paucinervis. The structures of the new compounds (1–3) were elucidated by 1D, 2D NMR spectra and HR ESIMS. In vitro antiproliferative activity against human promyelocytic leukemia HL-60 cells was tested, among which, compounds 2, 5, 6 and 7 exhibited strong growth inhibitory effects with GI50 values ranging from 1.30 to 9.08 lM, respectively. Preliminary SARs were also discussed.
Graphitization of graphene oxide films under pressure
Chen, Xianjue,Deng, Xiaomei,Kim, Na Yeon,Wang, Yu,Huang, Yuan,Peng, Li,Huang, Ming,Zhang, Xu,Chen, Xiong,Luo, Da,Wang, Bin,Wu, Xiaozhong,Ma, Yufei,Lee, Zonghoon,Ruoff, Rodney S. Elsevier 2018 Carbon Vol.132 No.-
<P><B>Abstract</B></P> <P>Lightweight, flexible graphite foils that are chemically inert, high-temperature resistant, and highly electrically and thermally conductive can be used as component materials in numerous applications. “Graphenic” foils can be prepared by thermally transforming graphene oxide films. For this transformation, it is desirable to maintain a densely packed film structure at high heating rates as well as to lower the graphitizing temperatures. In this work, we discuss the pressure-assisted thermal decomposition of graphene oxide films by hot pressing at different temperatures (<I>i.e.</I>, 300 °C, 1000 °C, or 2000 °C). The films pressed at 1000 °C or 2000 °C were subsequently heated at 2750 °C to achieve a higher degree of graphitization. The combination of heating and pressing promotes the simultaneous thermal decomposition and graphitic transformation of G-O films. Films pressed at 2000 °C as well as films further graphitized at 2750 °C show high chemical purity, uniformity, and retain their flexibility. For films pressed at 2000 °C and then further heated at 2750 °C, the mechanical performances outperform the reported values of the “graphite” foils prepared by calendering exfoliated graphite flakes; the electrical conductivity is ∼3.1 × 10<SUP>5</SUP> S/m and the in-plane thermal conductivity is ∼1.2 × 10<SUP>3</SUP> W/(m·K).</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
An On-Machine and Vision-Based Depth-Error Measurement Method for Micro Machine Tools
Shih-Ming Wang,Han-Jen Yu,Shih-His Liu,Da-Fan Chen 한국정밀공학회 2011 International Journal of Precision Engineering and Vol. No.
The error measurement method is an essential for further enhancing the machining accuracy of a micro machine tool. An on-machine vision-based measurement method that can measure 2-D contouring-/tracking errors of a micro machining process had been previously developed. An on-machine depth-error measurement method was proposed in this study to fulfill the complete 3-D machining errors measurement. The method adopts image re-constructive technology and camera pixel correction to provide non-contact measurement capability. To improve the measurement limits due to the pixel resolution and the filler of view of a CCD, a 2-step measurement method with use of a depth model was developed. Because of the capability of eliminating the shadow effects caused by the tilting light source, the proposed method provides more accurate and reliable measurement results. Sensitivity analysis was conducted to assess the influence of the CCD setup errors on the measurement accuracy for implementation. Experiment was conducted and the results have shown the effectiveness and feasibility of the measurement method.
Li, Da-Peng,Du, Chen,Zhang, Zuo-Ming,Li, Guang-Xiao,Yu, Zhi-Fu,Wang, Xin,Li, Peng-Fei,Cheng, Cheng,Liu, Yu-Peng,Zhao, Ya-Shuang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12
The present systematic review and meta-analysis was conducted to assess any association between breastfeeding and the risk of ovarian cancer. A systematic search of published studies was performed in PUBMED and EMBASE and by reviewing reference lists from retrieved articles through March 2013. Data extraction was conducted independently by two authors. Pooled relative risk ratios were calculated using random-effect models. Totals of 5 cohort studies and 35 case-control studies including 17,139 women with ovarian cancer showed a 30% reduced risk of ovarian cancer when comparing the women who had breastfed with those who had never breastfed (pooled RR = 0.70, 95% CI: 0.64-0.76; p = 0.00), with significant heterogeneity in the studies (p = 0.00; I2 = 76.29%). A significant decreasd in risk of epithelial ovarian cancer was also observed (pooled RR = 0.68, 95% CI: 0.61-0.76). When the participants were restricted to only parous women, there was a slightly attenuated but still significant risk reduction of ovarian cancer (pooled RR = 0.76, 95% CI: 0.69-0.83). For total breastfeeding duration, the pooled RRs in the < 6 months, 6-12 months and > 12 months of breastfeeding subgroups were 0.85 (95% CI: 0.77-0.93), 0.73 (95% CI: 0.65-0.82) and 0.64 (95%CI: 0.56-0.73), respectively. Meta-regression of total breastfeeding duration indicated an increasing linear trend of risk reduction of ovarian cancer with the increasing total breastfeeding duration (p = 0.00). Breastfeeding was inversely associated with the risk of ovarian cancer, especially long-term breastfeeding duration that demonstrated a stronger protective effect.