발효콩 추출물의 항돌연변이원성 및 세포독성 효과

최승필,이효진,문선영,김수현,이득식,함승시 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.3

This study was carried out to determine the antimutagenic and anticancer effects of fermented soybean using Ames test and cytotoxicity, respectively. The ethyl acetate fraction (200 g/plate) of fermented soybean in the Salmonella typhimurium TA100 strain showed 86.6% of inhibition rate against the mutagenesis induced by N-methyl-N-nitro-N-nitrosoguanidine(MNNG). In addition, the suppression of ethyl acetate fraction with same concentration of fermented soybean in the Salmonella typhimurium TA98 and TA100 strains showed 82.4% and 90.8% inhibition against 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxicity effects of fermented soybean against the cell lines with human lung carcinoma (A549), human gastric carcinoma (AGS) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 ㎎/mL ethyl acetate fraction of fermented soybean showed strong cytotoxicities of 71.6%, 91.5% and 80.7% against A549, AGS and MCF-7, respectively.

가시오갈피 열매 추출물의 항돌연변이원성 및 세포독성 효과

전윤영,최승필,이효진,문선영,이득식,함승시 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

가시오갈피(Eleutherococcus senticous Maxim) 열매의 에탄올 추출물 및 분획물의 항돌연변이성 및 항암활성을 규명하였다. 항돌연별이원성 실험결과에서는 직접변이원인 MNNG, 4NQO 그리고 간접변이원인 B(α)P, Trp=P-1에 대해서 농도의존적인 돌연변이 억제활성을 나타내었다. MNNG (0.4㎍/plate)의 경우 S. typhimurium TA100 균주에서는 시료농도 200㎍/plate에서 클로로포름 분획물을 제외한 모든 분획물들에서 90%이상의 높은 억제효과를 나타내었다. 4NQO(0.15㎍/plate)에 대한 S. typhimurium TA98 균주의 경우 물 분획물의 경우 88.3%, TA100 균주에서는 에틸 아세테이트 분획물이 84.4%의 억제효과를 보였다. 간접변이원의 경우 B(α)P(10㎍/plate)에선느 에탄올 추출물과 핵산 분획물은 시료농도 200㎍/plate에서 각각 96.1%와 97.5%로 가장 높은 억제효과를 나타내었다. 그리고 Trp-P-1(0.15㎍/plate)에서는 TA100 균주의 경우 200㎍/plate 농도에서 에탄올 추출물과 물 분획물이 각각 95.5%와 90%로 높은 억제율을 보여주었고, TA98 균주에 대해서는 에틸아세티에트 분획물이 88.3%로 비교적 높은 억제효과를 나타내었다. 인간의 암세포인 폐암세포(A549), 위암세포(AGS), 간암세포(Hep3B), 유방암세포(MCF-7)에 대한 세포독성 억제 효과를 검토한 결과 모든 암세포에서 각분획물들은 1㎎/mL 농도에서 60% 이상의 비교적 높은 암세포 성장 효과를 나타내었다. 특히 MCF-7에서 높은 암세포 성장억제 효과를 나타내었는데 핵산 분획물(1㎎/mL)에서 92.7%의 높은 암세포 성장 억제 효과를 나타내었고 Hep3B에서는 부탄을 분획물(1㎎/mL)에서 82%의 비교적 높은 성장 억제효과를 나타내었다. This study was carried out to investigate the antimutagenic and cytotoxic effects of Eleutherococcus senticosus Maxim fruits ethanol extract on Salmonella typhimurium TA98, TA 100 and cancer cell lines using Ames test and SRB assay, respectively. They were extracted with ethanol and then fractionated with hexane, chloroform, ethyl acetate, butanol and water to get active fractions. In the Ames test, most of the extract had strong antimutagenic effects against the mutagenesis induced by MNNG, 4NQO, B(α)P Trp-P-1. The ethanol extract (200 ㎍/plate) of Eleutherococcus senticosus fruits showed 87.2% inhibitory effect on the mutagenesis induced by MNNG against TA100. And also, The suppression ratio against B(α)P and AGS, MCF-7, Hep3B), the value value of inhibition were mostly above 60% for each fraction (1 ㎎/mL). Hexane fraction (1 ㎎/mL) against showed the strongest cytotoxic effects of 92.7% compared to those of other fraction and butano fraetion against Hep3B was relatively high growth inhibitory effect of 82%.

A Population-based Case-control Study on Risk Factors for Gastric Cardia Cancer in Rural Areas of Linzhou

Sun, Chang-Qing,Chang, Yu-Bo,Cui, Ling-Ling,Chen, Jia-Jun,Sun, Nan,Zhang, Wei-Jie,Jia, Xiao-Can,Tian, Yuan,Dai, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Gastric cancer is the second most common cause of cancer-related deaths in the world. Although certain dietary factors and lifestyles have been suggested to be associated with gastric carcinogenesis, there have been few investigations focusing on rural areas. A case-control study was therefore carried out to investigate the risk factors of gastric cardia cancer (GCC) in rural areas of Linzhou. A total of 470 newly diagnosed cases of GCC and 470 healthy controls were included. Face-to-face interviews were conducted, using a uniform questionnaire containing questions on demographics, per capita income, living habits, dietary habits and family history of tumors. The relationship between putative risk factors and GCC was assessed by odds ratios (OR) and their 95% confidence intervals (95%CI) derived from conditional logistic regression model by the COXREG command using SPSS 12.00. Multivariate logistic regression analysis was used to evaluate simultaneously the effects of multiple factors and other potential confounding factors. Multivariate logistic analysis showed that smoking (OR=1.939, 95%CI:1.097-3.426), alcohol drinking (OR=2.360, 95%CI: 1.292-4.311), hot food consumption (OR=2.034, 95%CI: 1.507-2.745), fast eating (OR=1.616, 95%CI: 1.171-2.230), mouldy food (OR=4.564, 95%CI: 2.682-7.767), leftover food (OR=1.881. 95%CI: 1.324-2.671), and family history of tumor (OR=2.831, 95%CI: 1.588-5.050) were risk factors for GCC. High per capita income (OR=0.709, 95%CI: 0.533-0.942), high education level (OR=0.354, 95%CI: 0.163-0.765), consumption of fresh fruits (OR=0.186, 95%CI: 0.111-0.311) and vegetables (OR=0.243, 95%CI: 0.142-0.415), and high BMI (OR=0.367, 95%CI: 0.242-0.557) were protective factors for GCC. Our data indicate that unhealthy lifestyle and dietary habits might be important contributors to GCC in this population.

Identifying MicroRNA and mRNA Expression Profiles in Embryonic Stem Cells Derived from Parthenogenetic, Androgenetic and Fertilized Blastocysts

Cui, Xiang-Shun,Shen, Xing-Hui,Sun, Shao-Chen,Cho, Sun-Wha,Heo, Young-Tae,Kang, Yong-Kook,Wakayama, Teruhiko,Kim, Nam-Hyung Elsevier 2013 Journal of genetics and genomics Vol.40 No.4

<P>MicroRNAs (miRNAs) are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions. In this study, miRNA and messenger RNA (mRNA) profiles were examined by Illumina microarray in mouse embryonic stem cells (ESCs) derived from parthenogenetic, androgenetic, and fertilized blastocysts. The global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene expression. Results showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs (aESCs) and fertilized ESCs (fESCs), a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs (pESCs) and fESCs, and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and pESCs. In addition, a total of 575, 5 and 376 miRNA-mRNA target pairs were observed in aESCs vs. fESCs, pESCs vs. fESCs, and aESCs vs. pESCs, respectively. Furthermore, 15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time RT-PCR. Finally, transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression of Peg3, Dyrk1b, and Prrg2 mRNA, inhibition of miR-363 increased the expression of Nfat5 and Soat1 mRNA, and inhibition of miR-883b-5p increased Nfat5, Tacstd2, and Ppapdc1 mRNA. These results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.</P>

A phosphorene–graphene hybrid material as a high-capacity anode for sodium-ion batteries

Sun, Jie,Lee, Hyun-Wook,Pasta, Mauro,Yuan, Hongtao,Zheng, Guangyuan,Sun, Yongming,Li, Yuzhang,Cui, Yi Nature Publishing Group 2015 Nature nanotechnology Vol.10 No.11

Sodium-ion batteries have recently attracted significant attention as an alternative to lithium-ion batteries because sodium sources do not present the geopolitical issues that lithium sources might. Although recent reports on cathode materials for sodium-ion batteries have demonstrated performances comparable to their lithium-ion counterparts, the major scientific challenge for a competitive sodium-ion battery technology is to develop viable anode materials. Here we show that a hybrid material made out of a few phosphorene layers sandwiched between graphene layers shows a specific capacity of 2,440 mA h g<SUP>−1</SUP> (calculated using the mass of phosphorus only) at a current density of 0.05 A g<SUP>−1</SUP> and an 83% capacity retention after 100 cycles while operating between 0 and 1.5 V. Using in situ transmission electron microscopy and ex situ X-ray diffraction techniques, we explain the large capacity of our anode through a dual mechanism of intercalation of sodium ions along the x axis of the phosphorene layers followed by the formation of a Na<SUB>3</SUB>P alloy. The presence of graphene layers in the hybrid material works as a mechanical backbone and an electrical highway, ensuring that a suitable elastic buffer space accommodates the anisotropic expansion of phosphorene layers along the y and z axial directions for stable cycling operation.

A Genetic Heterogeneity of Renpenning Syndrome Mapped to Chromosome Xq21-Xqter

Cui, Bin,Sun, Yuming,Sun, Yuqin,Shi, Yuefeng,Pei, Gang,Kong, Xiangyin,Hu, Landian 한국유전학회 2004 Genes & Genomics Vol.26 No.1

Renpenning sysndrome is an X-linked mental retardation associated with short stature, moderate microcephaly, unremakdable facies, and no other neurological abnormality. Renpenning first reported this disorder in a Mennonite family, and using this family, Renpenning Syndrome (RENS) has been mapped to Xp11.2-p11.4 Xu Cs found a Chinses family with an X-linked hereditary disease, and the clinical phenotype is similar to RENS. Recently, we performed the linkage analysis, and the result has shown ht egenetic heterogeneity of Renpenning Syndrome: a suggestive novel locus mapped to Xq21-Xqter in this Chinese family.

RALY RNA Binding Protein-like Reduced Expression is Associated with Poor Prognosis in Clear Cell Renal Cell Carcinoma

Cui, Zhi-Wen,Xia, Ye,Ye, Yi-Wang,Jiang, Zhi-Mao,Wang, Ya-Dong,Wu, Jian-Ting,Sun, Liang,Zhao, Jun,Fa, Ping-Ping,Sun, Xiao-Juan,Gui, Yao-Ting,Cai, Zhi-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

The molecular mechanisms involved in the progression of clear cell renal cell carcinomas (ccRCCs) are still unclear. The aim of this study was to analyse the relationships between expression of RALYL and clinical characteristics. In 41 paired samples of ccRCCs and adjacent normal tissues, we used real-time qPCR to evaluate the expression of RALYL mRNA. RALYL protein levels were determined in 146 samples of ccRCC and 37 adjacent normal tissues by immunohistochemistry. Statistical analysis was used to explore the relationships between expression of RALYL and the clinical characteristics (gender, age, tumor size, T stage, N stage, M stage, survival times and survival outcome) in ccRCC. In addition, these patients were follow-up period 64 months (range: 4~116months) to investigate the influence on prognosis. We found significantly differences between ccRCC tissues and normal tissues (p<0.001, paired-sample t test) in mRNA levels of RALYL. Immunohistochemistry analyses in 146 ccRCC samples and 37 adjacent normal tissues showed significantly lower RALYL protein levels in ccRCC samples (${\chi}^2$-test, p<0.001), inversely correlating with tumour size (p=0.024), T stage (0.005), N stage (p<0.001) as well as M stage (p=0.019), but not age (p=0.357) and gender (p=0.348). Kaplan-Meier survival analysis demonstrated that people with lower level of RALYL expression had a poorer survival rate than those with a higher level of RALYL expression, significantly different by the log-rank test (p=0.011). Cox regression analysis indicated that RALYL expression (p=0.039), N stage (p=0.008) and distant metastasis (p<0.001) were independent prognosis factors for the overall survival of ccRCC patients. We demonstrated that the expression of RALYL was significantly low in ccRCC and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic factor.

Pronuclear Formation and DNA Synthesis in Pig Oocytes following Intracytoplasmic Injection of Pig or Mouse Spermatozoon

Xiang Sun Cui,Bong-Ki Kim,Sun Hong Jun,Dong Il Jin,Chang Sik Park,Nam-Hyung Kim 한국동물번식학회 2002 Reproductive & Developmental Biology(Supplement) Vol.26 No.1s

Impurity Behaviors under Wall Conditioning in HL-2A

Zhengying Cui,Chenghe Cui,Mingxu Wang,Ping Sun,Quanming Wang,Wei Li,Yudong Pan,Zeng Cao 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.III

Impurities are one of the key issues of tokamak plasma research, as they are directly related to plasma quality and steady-state operation. The HL-2A device with two close divertor is the first divertor tokamak in China. It was successfully constructed in 2002 and has been operated for three 3 years up to now. In the 2004 campaign, siliconization as a wall conditioning was first done on the HL-2A tokamak by using glow discharge cleaning (GDC) with a gas mixture of SiH4 + He. The effects of siliconization on impurities and recycling are investigated, as well as the lifetime of siliconization, in this paper. The intensity of oxygen line is remarkably decreased after siliconization. Radiation of silicon line rapidly decreases shot by shot just after siliconization, but the effect of siliconization on the plasma properties is present all along and decreases gradually for a large amount of discharges. The effect of the siliconization can be maintained to about 180 discharges with similar discharge parameters.

연구개발과 마케팅 부문 간 공유영역의 조직 구조적 특성과 구성요인이 신제품개발 성과에 미치는 영향에 관한 탐색적 연구: 한국 기업과 중국 기업의 비교분석을 중심으로

손취걸 ( Cui Jie Sun ),박주홍 ( Joo Hong Park ) 한독경상학회 2011 經商論叢 Vol.29 No.2

본 연구는 신제품개발에 있어서 연구개발과 마케팅 부문 간 공유영역의 조직 구조적 특성(집중화, 공식화)이 공유영역의 구성요인(커뮤니케이션 원활성, 신제품개발 참여 정도, 부서장 파워)과 신제품개발 성과(신제품개발 건수/특허, 실용신안 및 의장건수)에 미치는 영향, 조직 구조적 특성이 신제품개발 성과에 미치는 영향, 그리고 조직 구조적 특성과 공유영역의 구성요인이 신제품개발 성과에 미치는 영향을 실증적으로 분석하였다. 실증적 분석을 위하여 한국 기업과 중국 기업을 대상으로 한 설문조사가 실시되었다. 우편 및 방문조사를 통하여 회수된 설문지 중에서 극단적인 응답을 한 설문지를 제외하고, 중국에서 회수된 57부, 한국에서 회수된 37부가 최종 실증분석 자료로 이용되었다. 측정변수의 신뢰성과 타당성을 검정한 후, 다중회귀분석을 통하여 가설을 검정하였다. 국가별 가설검정 결과를 바탕으로 본 연구는 이론적 및 관리적 시사점을 제공한다. This paper attempts to analyze impacts of the interface components between R&D and marketing on the output of new product development. Especially, this study analyzed these impacts in Korean and Chinese firms. For the empirical research, we selected variables, i.e. organization structural characteristics(the degree of the centralization between the R&D and marketing, the degree of the formalization between the R&D and marketing), smooth communication, the degree of participation between the R&D and marketing during the process of new product development, and the power of the head of the department, which could influence on the output of new product development. Based on these factors, research objects were formulated as follows: 1) To analyze how these factors have an effect on the output of new product development; 2) To analyze how these factors have an effect on the output of new product development in the Korean firms; 3) To analyze how these factors have an effect on the output of new product development in the Chinese firms. For empirical research, we conducted mail survey and visit. 57 Chinese and 37 Korean questionnaires were used for empirical analysis. Data were analyzed by using Cronbach`s alpha, factor analysis and multiple regression analysis utilizing SPSS 16.0 statistical package. The results of the empirical analysis showed that factors influenced on the output of new product development and have effects on the output of new product development in Korean and Chinese firms differently. Based on the results, we provide theoretical and managerial implications. To maximize the effect of new product development, the interface between the R&D and marketing must interact through the new product development project. This is the key to new product development success. However, there are some limitations in this study. The sample size is relatively small. So it can be connected with imperfect results. Therefore, we need more refined methodology for the future study. And to get more correct results, the number of sample should be increased.