한국잔디 수집계통들 중에서 우수계통들의 생육특성 비교

임용우(Y. W. Rim),김기용(K. Y. Kim),김맹중(M. J. Kim),성병렬(B. R. Sung),임영철(Y. C. Lim),정의수(E. S. Chung),신홍균(H. K. Shin),김용선(Y. S. Kim) 한국잡초학회·한국잔디학회 2003 Weed & Turfgrass Science Vol.17 No.2,3

2001년 수집된 한국잔디 133계통들 중에서 우수한 계통들을 선발하기위하여 밀도(품질),<br/> 피복성, 녹색기간, 내병성, 출수유무(종자수) 등의 주요특성과 그 외 생육특성들이 조사되었다. 생육특성이 우수한 6계통이 선발되었으며 그 결과를 요약하면 다음과 같다.<br/> 수집된 133계통들 중에서 가장 녹색기간이긴 계통은 J01067으로 11월초까지 녹색도를 유지하였다. J01106 및 J01129 계통들은 엽폭이 각각 1.5mm 및 2mm 정도로 매우 좁으며, 밀도가 높고, 피복성도 좋은 것으로 나타났으며, 내병성도 강하였다. J01122 계통은 들잔디 계통들 중에서 유일하게 내병성이 강한 계통으로 선발되었다. J01128 계통은 엽폭이 3.2mm로 중엽에 속하였으며, 대비품종인 Sunburst 와 비슷하였다. 이 계통의 경우도J01106, J01129 계통들과 마찬가지로 밀도가 높고 내병성이 강한 것으로 나타났다.<br/> 수집된 133계통들의 형태적 분류를 위하여 5가지의 생육특성을 사용하여 크게 세가지 군집<br/> 으로 분류하였으며, 그 중에서 대비품종으로 사용된 6품종들과 선발된 우수 6계통의 분류는<br/> 다음과 같다. 제 1군집에 속하는 품종 및 계통은 Belare, Meyer, 안양중지(Anyang-jungji), J01067, J01112 등이 포함되었으며, 제 2군집으로는 S-94, J01105가 포함되었고, 제 3군집에는 Sunburst, 건희(Konhee), J01106, J01128, J01129 등이 포함되었다. Growth characteristics such as density(quality), covering speed, green period, disease resistance, heading existence(number of seeds) and other characteristics were examined for selection of superior lines among the 133 zoysiagrass lines collected in 2001. Six superior lines were selected and the results were summarized as follows.<br/> Superior line, J01067 was longest for green period among the 133 zoysiagrasses and stayed green until the beginning of November. Leaf width of J01106 and J01129 lines was very narrow as 1.5㎜ and 2㎜, respectively and superior for density(quality), covering speed and disease resistance. J01122 line was selected for strongest disease resistance among the lines of Zoysia japonica. Leaf width of J01128 was 3.2㎜ showing midium type and similar to standard cultivar, Sunburst. This line also showed higher density and strong disease resistance like J01106 and J01129.<br/> Five growth characteristics for morphological classification of 133 zoysiagrass lines<br/> was used and divided into 3 cluster groups. Of 133 lines, 6 standard cultivars and 6 superior lines were classified as follows. First cluster group contained Belare, Meyer, Anyang-jungji, J01067, J01122, and second group contained S-94 and J01105, and third group contained Sunburst, Konhee, J01106, J01128 and J01129.<br/> <br/>

돼지 卵子의 透明帶에 대한 單一클론抗體生産과 그 特性에 關한 硏究

金鐘培,劉永春,金昌圭,權五中,鄭盛元,鄭吉生 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

本 試驗은 單一클론抗體의 강한 特異性과 抗體性質의 不變性을 이용하여 發生學的 側面에서 哺乳動物 卵子의 透明帶의 機能과 構造를 이해하고, 또한 種特異的인 精子 受容體의 存在 및 生化學的 構造를 규명하기 위한 기초연구로서, 돼지 卵子의 透明帶를 免疫原으로 하여 BABL/c 생쥐로부터 單一클론抗體를 생산하고 그 특성을 구명하였던 바 다음과 같은 결과를 얻었다. 1) 3마리의 BABL/c 생쥐(YⅠ, YⅡ, ZI)에 돼지卵子의 透明帶를 免疫化하고, 複合抗體 生成을 확인한 후 생쥐의 脾臟細胞와 Myeloma(SP2/O-Ag14)를 polyethylene glycol를 融合을 실시한 결과 각각 25.8%, 54.5% 그리고 59.7%의 融合效率을 나타내었으며, ELISQ에 의해 陽性反應을 조사한 결과 각각 17.3%, 32.6% 그리고 6.2% 陽性反應 效率을 나타내었다. 2) YI에서 강한 陽性反應을 보인 6개의 well에 대한 cloning을 실시하고 抗體檢證을 행한 결과 20.8% ∼ 48.4%의 Cloning效率과 54.6% ∼ 82%의 陽性反應 效率을 나타내었다. 3) 강한 陽性反應을 나타낸 항체에 대해 sandwich ELISA法에 의해 isotype을 決定하였던바 2E93C(YⅠ), 3E83E7(YⅠ), 4E3(YⅡ)각각 IgG₂b, IgG₂a, IgM으로 확인되었다. 4) Isotype이 決定된 2E93C9(YⅠ), 3E84E7(YⅠ), 4E3(YⅡ)의 세포를 생쥐의 腹腔에 주사하여 얻은 腹水를 indirect ELISA法에 의해 titer를 決定한 결과 모두 1:400,000 이상의 높은 titer를 나타내었다. 5) 處理區로서 單一클론抗體의 腹水와 對照區로서 normal mouse serum이 각각 2%씩 함유된 배양액속에서 난자를 배양한 후 顯徵鏡下에서 관찰했을 때 對照區에서 배양된 난자의 표면은 정상적인 형태를 나타냈으나 處理區에서 배양된 卵子는 표면에 뚜렷한 沈澱層을 형성하였다. 6) 處理區와 對照區 卵子를 Rabbit anti-mouse IgG-FITC가 1% 함유된 배양액속에서 배양하고 洗滌한 후 螢光顯徵鏡下에서 관찰한 바 處理區의 卵子는 透明帶 주위에서 螢光이 나타났으나, 對照區에서는 나타나지 않았다. This study was carried out ot produce and characterize monoclonal antibodies against porcine zona pellucida, and undertaken as a basic study to develop immunocontraceptive vaccine and to investigate the function of zana pellucida in early fertilization process. The results obtained were summarized as follows: 1. Spleen cells of three BALB/C mice(YⅠ, YⅡ and ZI) which were immunized with porcine zona pelucida were fused with myeloma cells(SP2/O-Ag14) by polyethylene glycol. At the result of fusion, fusion efficiency was 25.8 , 54.5% and 59.7% and positive efficiency 17.3%, 32.6% and 6.2%, respectively. 2. Cloning efficiency was shown to be from 20.8% to 48.4% and positive efficiency of them were 54.6% to 82%. 3. Sub-isotypes of three strong positive antibodies, 2E93C(YⅠ), 3E83E7(YⅠ) and 4E3(YⅡ) were determined by sandwich ELISA method and shown to be as IgG2b, IgH2a and IgM, respectively. 4. The titers of three ascitic fluids containing antibodies, 2E93C9(YⅠ), 3E84E7(YⅠ) and 4E3(YⅡ) were determined by indirect ELISA and all of them showed above 1:400,000. 5. The layer of precipitate was observed on the zona incubated with medium containing 2% ascitic fluid of monoclonal antibody while the eggs treated with 2% normal mouse serum did not. 6. Porcine eggs incubated with medium containing 2% ascitic fluid of monoclonal antibody and followed by subsequent incubation with Rabbit anti-mouse IgG-FITC conjugate showed strong fluorescent light on the zona surface while the zona of normal mouse serum-treated eggs did not show fluorescence.

The antimicrobial peptide human cationic antimicrobial protein-18/cathelicidin LL-37 as a putative growth factor for malignant melanoma

Kim, J.E.;Kim, H.J.;choi, J.M.;Lee, K.H.;Kim, T.Y.;Cho, B.K.;Jung, j.Y.;Chung, K.Y.;Cho, D.;Park, H.J. Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

Background Recent evidence suggests cathelicidin LL-37 to be a growth factor for various human cancers such as lung cancer, ovarian cancer and breast cancer. However, the effect of LL-37 against malignant skin cancer has not been reported. Objectives To investigate whether the human cathelicidin LL-37 is involved in the carcinogenesis of various skin tumours. Methods Human cationic antimicrobial protein-18 (hCAP-18)/LL-37 production in several cell lines including HaCaT, a chronic myelogenous leukaemia (CML) cell line and various melanoma cell lines was examined using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Immunohistochemical analysis of melanoma, nonmelanoma skin cancer and precancerous and benign skin lesions was performed. After adding LL-37 to a melanoma cell line, tumour cell proliferation, migration and invasion were investigated. Results Human malignant melanoma cell lines overexpressed hCAP-18/LL-37 mRNA and peptide compared with HaCaT and CML cell lines. Immuno- histochemistry showed that the peptide was strongly expressed in malignant melanoma and moderately expressed in squamous cell carcinoma, whereas basal cell carcinoma, precancerous lesions and seborrhoeic keratosis showed no or weak expression. LL-37 also stimulated melanoma cell proliferation, migration and invasion in vitro. Conclusions Cathelicidin LL-37 was primarily expressed in human malignant skin cancer. LL-37 promoted melanoma cell proliferation, migration and invasion in vitro. We report that an increase in the level of LL-37 is associated with malignant skin tumours such as malignant melanoma. These results highlight the importance of LL-37 in the malignant tendency of skin tumours.

[디젤엔진부문] 이종연료 층상분사를 이용한 디젤엔진의 NOx와 soot 동시 저감에 관한 연구

강병무(B.M. Kang),김종률(J.Y. Kim),이태원(T.W. Lee),정성식(S.S Chung),하종률(J.Y. Ha) 한국자동차공학회 2000 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

<br/> ~~.A\-%7}t'--c:j-~ 2000\15:. ~;>jI~t:1J~<br/> 2000-03-2041<br/> 01<br/> 3S. 91 ~ ~A~~<br/> A<br/> L.s:.<br/> OI<br/> Ho~<br/> c:"~.n. C::>C>c r2. C:::>L ;<br/> C/~2!!~~ NOx$ soot ~AI AI~OU ~~ eF?<br/> 7J'!!-';'-, 7,J'6-it(%ottJI-o:}jj! tJI~~), 01I11~(~~ ~~tJI-O:}), '\J'\J-6j, "6J-'6-iH%ottJI-o:}jj!)<br/> A Study on the Simultaneous Reduction of :>iOx and Soot<br/> with Dual Fuel Stratified Injection System in a Diesel Engine<br/> B. 1\1. Kang, ]. Y. Kim(Graduated School of Dong- A L'niv'>, T. W. Lee(Changwon CQUage), S. S. Chung, ]. Y. Ha(Dong-A Univ,)<br/> ABSTRACT<br/> To investigate the method of the simultaneous reduction of Soot and NOx, we developed a new conception for dual fuel stratified injection system in a Diesel Engine.<br/> Some parts of the injection system, ie. nozzle holder, delivery vale. were remodeled to inject diesel and methanol in order from one injector. The Quantity of diesel and methanol was controled precisely by micrometers that were mounted at control lack of injection pump.<br/> The real injection Quantity ratio of dual fuel was certificated by volumetric ratio at injection Quantity experiment. We could confirm the capability that soot and NOx simultaneously were reduced by diesel-methanol stratified injection from the results of combustion and exhaust characteristic.<br/>

The pharmacological stimulation of Nurr1 improves cognitive functions via enhancement of adult hippocampal neurogenesis

Kim, J.i.,Jeon, S.G.,Kim, K.A.,Kim, Y.J.,Song, E.J.,Choi, J.,Ahn, K.J.,Kim, C.J.,Chung, H.Y.,Moon, M.,Chung, H. Elsevier 2016 Stem cell research Vol.17 No.3

<P>The nuclear receptor related-1 (Nurr1) protein plays an important role in both the development of neural precursor cells (NPCs) and cognitive functions. Despite its relevance, the effects of Nurr1 on adult hippocampal neurogenesis have not been thoroughly investigated. Here we used RT-PCR, western blot, and immunocytochemistry to show that adult hippocampal NPCs abundantly express Nurr1. We then examined the effect of Nurr1 activation on adult hippocampal NPCs using amodiaquine (AQ), an anti-malarial drug that was recently discovered to be a Nurr1 agonist. Cell proliferation assay showed that AQ significantly increased cell proliferation. AQ-treated NPCs showed increased levels of phosphorylation of Akt and ERK1/2 whereas AQ-treated Nurr1 siRNA-transfected NPCs showed no changes in those levels. Further immunocytochemical and immunohistochemical analyses confirmed the stimulating effect of Nurr1 agonist on the proliferation and differentiation of adult hippocampal NPCs both in vivo and in vitro. In addition to its effects on proliferation and differentiation of NPCs, AQ-treated mice showed a significant enhancement of both short-and long-term memory in the Y-maze and the novel object recognition test. These data suggest that activation of Nurr1 may enhance cognitive functions by increasing adult hippocampal neurogenesis and also indicate that Nurr1 may be used as a therapeutic target for the treatment of memory disorders and cognitive impairment observed in neurodegenerative diseases. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.</P>

Pretreatment HBeAg level and an early decrease in HBeAg level predict virologic response to entecavir treatment for HBeAg‐positive chronic hepatitis B

Kwon, J. H.,Jang, J. W.,Lee, S.,Lee, J.,Chung, K. W.,Lee, Y. S.,Choi, J. Y. Blackwell Publishing Ltd 2012 Journal of viral hepatitis Vol.19 No.2

<P><B>Summary. </B> There are few reports on hepatitis B e antigen (HBeAg) titres during nucleos(t)ide analogues treatment. We investigated the changes in HBeAg levels in patients treated with entecavir and the usefulness of HBeAg quantification for predicting antiviral response. Ninety‐five consecutive HBeAg‐positive patients treated with entecavir for more than 48 weeks were enrolled. Serum levels of hepatitis B surface antigen (HBsAg), HBeAg and HBV DNA were assessed at 4‐week intervals to week 24 and thereafter at 12‐week intervals. Virologic response (Y1VR) was defined as an undetectable HBV DNA level at week 48 of therapy. During 48 weeks, HBeAg and HBV DNA level decreased significantly in a biphasic manner and HBsAg level tended to decease. Fifty‐three patients (55.8%) attained Y1VR. Pretreatment HBeAg levels were significantly lower in the Y1VR group than in no Y1VR group. At week 4 and 12 of therapy, 25% and 41.4% of patients showed a decrease of HBeAg levels with >0.5 log<SUB>10</SUB> and >1.0 log<SUB>10</SUB> from baseline, respectively. These patients achieved more Y1VR than those with less decrease of HBeAg levels (97.7%<I>vs</I> 22.2% and 86.2%<I>vs</I> 29.3%, respectively). HBeAg level at week 12 had higher predictive values for Y1VR than HBV DNA level. Multivariate analysis revealed that a pretreatment HBeAg level of <360 PEIU/mL and the reduction in HBeAg level >1.0 log<SUB>10</SUB> at week 12 were associated with Y1VR. These results suggest that pretreatment HBeAg level and an early decrease in HBeAg level are useful measurements for predicting one‐year virologic response during entecavir treatment.</P>

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

고무 및 화학제품제조업 산업장의 작업환경 실태에 관한 조사연구

전종휘,김용완,배기택,김준연,김진옥,김준효 인제대학교 1980 仁濟醫學 Vol.1 No.2

고무 혹은 화학제품제조업 산업장내 각종 유해작업환경 인자 가운데 특히 톨루엔ㆍ키실렌 등과 같은 유기용제가 산업보건학적 견지에서 보아 중요한 유해인자로 고려되었다. 따라서 고무 혹은 화학제품제조업 산업장과 그 근로자들에 대한 보건관리사업은 유기용제 중독의 예방과 조기치료에 중점을 두어야 할 것으로 생각된다. This study was conducted on some rubber and chemical products manufacturing industries of relatively larger sires and 3,356 workers in Busan area over a period of 4 months from June 1 to ,September 30, 1979. The purpose of the study was in investigating the status of working environment in rubber and chemical products manufacturing industries. The summarized results were as follows: 1.The highest and lowest mean values of harmful environmental elements in workroom of rubber products manufacturing industries were noted in cutting process (93.8 dB) and decorative designing(72.3 dB) in case of noise, vulcanizing (6.92 mg/m3) and rolling (5.55 mg/m3) in class II dust, spreading (883.3 ppm and 803.3 ppm, respectively) and decorative designing (155.0 ppm and 93.3 ppm, respectively) in toluene and xylene, and spreading (87.3 ppm) and assembling (61.0 ppm) ill benzene, respectively. Also in chemical products manufacturing industries, they were noted in culling process (96.2 dB) and inspecting (74.3 dB) in case of noise, cutting (9.07 mg/m3) and grinding (2.30 mg/m3) in class II dust, casting (160.0 ppm) and tube (71.7 ppm) in toluene. printing (133.3 ppm and 43.5 ppm respectively) and tube (66.7 ppm and 11.7 ppm, respectively) in xylene and benzene, respectively. 2.The workers of rubber products manufacturing industries who exposed to working environment above threshold limit value of harmful elements were highly noted in pressing process, cutting and compressing in case of noise, mixing, cutting and vulcanizing in class II dust, decorative designing and spreading in toluene and benzene, and spreading in xylene, respectively. Also in chemical products manufacturing, they were highly noted in cutting process, sheet and grinding in case of noise, mixing and cutting in class II dust, and printing and milling in toluene. xylene and benzene, respectively.

만삭자궁파열 10예 : A Report of 10 Cases

정우열,문동열,이지재 대한산부인과학회 1974 Obstetrics & Gynecology Science Vol.17 No.10

Population structure analysis reveals the maintenance of isolated sub-populations of weedy rice

CHUNG, J-W,PARK, Y-J Blackwell Publishing Ltd 2010 Weed research Vol.50 No.6

<P><SMALL>CHUNG</SMALL> J-W & <SMALL>PARK</SMALL> Y-J (2010). Population structure analysis reveals the maintenance of isolated sub-populations of weedy rice. <I>Weed Research</I> <B>50</B>, 606–620.</P><P>Summary</P><P>This study was conducted to assess the genetic diversity, population structure and linkage disequilibrium (LD) of weedy rice in Korea using 73 weedy rice, 24 landrace lines, 12 breeding lines and 24 international varieties. In total, 772 alleles were detected in 63 microsatellite loci. The number of alleles per marker locus ranged from two to 40, with an average of 12.3. The gene diversity and polymorphism information content value averaged 0.694 and 0.668, with a range of 0.016–0.941 and 0.016–0.946 respectively. In a model-based analysis of population structure, Korean rice germplasm was classified into four subpopulations, designated ‘cultivated <I>indica</I>’, ‘cultivated <I>japonica</I>’, ‘weedy <I>indica</I>’, ‘weedy <I>japonica</I>.’ Pair-wise estimates of <I>F</I><SUB>ST</SUB> indicated different degrees of differentiation between the four model-based populations, with values ranging from 0.073 (between cultivated and weedy <I>japonica</I>) to 0.474 (between weedy <I>japonica</I> and weedy <I>indica</I>). The weedy <I>indica</I> population appeared to be highly differentiated, compared with the other populations. The cultivated <I>indica</I> lines had the highest gene diversity (0.58), followed by cultivated <I>japonica</I> (0.50), weedy <I>japonica</I> (0.42) and weedy <I>indica</I> (0.35). The total number of private alleles in weedy <I>indica</I> and <I>japonica</I> populations was 39 (23 markers) and 55 (22 markers) respectively. The average of LD (<I>r</I><SUP>2</SUP>) of weedy <I>indica</I> and <I>japonica</I> types was higher than that of the two cultivated populations, both for inter- and intrachromosome values, indicating the possible reproductive and geographical isolation of sub-populations in cultivated rice fields.</P>