새로운 번식기술

정길생 한국낙농육우협회 1985 낙농·육우 Vol.48 No.-

연재2/가금의 인공수정과 문제점 -특히 수정율을 중심으로

정길생 대한양계협회 1974 월간 양계 Vol.6 No.2

수정율 저하문제와 그 대책

정길생 대한양계협회 1974 월간 양계 Vol.6 No.3

수정율은 수탉이 노화하거나 내분비선에 이상이 있을 때, 정액을 너무 자주 채취할 때, 여름에걸쳐 가을까지, 수탉의 영양상태가 나쁠 때, 채취한 정액에 오줌이나 계분이 혼입되었을 때 정액주입이 부적당할 때, 희석액이 변질되었을 때, 떨어진다. 수정율을 높이기 위하여 필요한 사항은 무엇인가 알아보자.

축산학교육의 문제와 전망

정길생,Jeong, Gil-Saeng 한국대학교육협의회 1984 大學敎育 Vol.11 No.-

농업 및 식품 생명공학 기술분야 : 동물분야

정길생 한국과학기술원 유전공학연구소 1993 생명공학동향 Vol.1 No.4

건강 추출물의 뇌세포 보호 작용

정길생,리빈,이동성,최현규,김윤철 한국생약학회 2010 생약학회지 Vol.41 No.3

Glutamate-induced oxidative injury contributes to neuronal degeneration in many central nervous system (CNS)diseases, such as Parkinson’s disease, Alzheimer’s disease, epilepsy and ischemia. Inducible heme oxygenase (HO)-1 acts against oxidants that are thought to play a role in the pathogenesis of these diseases. NNMBS098, a composition comprising the water insoluble of the 70% EtOH extract of Zingiberis Rhizoma, showed the potent neuroprotective effects on glutamateinduced neurotoxicity by induced the expression of heme oxygenase (HO)-1 and increased HO activity in the mouse hippocampal HT22 cells. Furthermore, NNMBS098 caused the nuclear accumulation of nuclear factor E2-related factor 2 (Nrf2)in mouse hippocampal HT22 cells. In addition, we found that treatment with c-Jun N-terminal kinase (JNK) inhibitor (SP600125) reduced NNMBS098-induced HO-1 expression and NNMBS098 also increased JNK phosphorylation. Therefore,these results suggest that NNMBS098 increases cellular resistance to glutamate-induced oxidative injury in mouse hippocampal HT22 cells, presumably through JNK pathway-Nrf2-dependent HO-1 expression.

Roles of heme oxygenase-1 in curcumin-induced growthinhibition in rat smooth muscle cells

정길생,Sun-Oh Jeong,Hak Sung Kim,김순애,Youn-Chul Kim,Su-Jin Yoo,Heung-Doo Kim,정헌택 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.3

In vascular smooth muscle cells (VSMCs), inductionof the heme oxygenase-1 (HO-1) confers vascularprotection against cellular proliferation mainly viaits up-regulation of the cyclin-dependent kinaseinhibitor p21WAF1/CIP1 that is involved in negativeregulation of cellular proliferation. In the presentstudy, we investigated whether the phytochemicalcurcumin and its metabolite tetrahydrocurcumincould induce HO-1 expression and growth inhibitionin rat VSMCs and, if so, whether their antiproliferativeeffect could be mediated via HO-1 expression. Atnon-toxic concentrations, curcumin possessingtwo Michael-reaction acceptors induced HO-1 expressionby activating antioxidant response element(ARE) through translocation of the nuclear transcriptionfactor E2-related factor-2 (Nrf2) into thenucleus and also inhibited VSMC growth triggeredby 5% FBS in a dose-dependent manner. In contrast,tetrahydrocurcumin lacking Michael-reaction accep -tor showed no effect on HO-1 expression, AREactivation and VSMC growth inhibition. The antiproliferativeeffect of curcumin in VSMCs was accompaniedby the increased expression of p21WAF1/CIP1. Inhibition of VSMC growth and expression ofp21WAF1/CIP1by curcumin were partially, but notcompletely, abolished when the cells were coincubatedwith the HO inhibitor tin protoporphyrin. In human aortic smooth muscle cells (HASMCs),curcumin also inhibited growth triggered by TNF-αand increased p21WAF1/CIP1expression via HO-1-dependent manner. Our findings suggest that curcuminhas an ability to induce HO-1 expression,presumably through Nrf2-dependent ARE activation,in rat VSMCs and HASMCs, and provide evidence thatthe antiproliferative effect of curcumin is considerablylinked to its ability to induce HO-1 expression.

牛精漿의 各 劃分이 햄스터精子의 運動性 및 受精能에 미치는 影響

鄭吉生,尹和重 건국대학교 1983 學術誌 Vol.27 No.2

These experiments were performed to investigate the effect of fractions separated from bovine seminal plasma on the motility and activation of hamster spermatozoa. Hamster spermatozoa collected from caudal epididymis were incubated in fresh bovine seminal plasma, or culture medium containing fresh or heated fractions separated by dialysis from bovine seminal plasma, and changes in motility index, activation and capacitation of spermatozoa during culture were estimated. The results obtained were summarized as follows : (1) The motility of hamster spermatozoa were inhibited by fresh bovine seminal plasma and the inhibitory effect was not modified by the dilution of seminal plasma at 64 times wish BSA free TALP solution. (2) Fraction I separated by dialysis from bovine seminal plasma stimulated significantly (p < 0.05) the motility of hamster spermatozoa, but the reverse was true for the nondializable, water insoluble fraction II and water soluble fraction III. However, fraction III also stimulated the motility of hamster spermatozoa when the fraction was inactivated by heating at 70℃ for 30 minutes. (3) Fraction I was stable against heat treatment: its sperm motility stimulating activity was not changed after heating at 90℃ for 30 minutes, but that of fraction III was decreased significantly (p < 0.05) by similar heat treatment. (4) The optimum concentration of fraction III was 24mg/ml of BSA free TALP solution. The optimum combination of heated fraction I and fraction III for sperm motility was found to be 12㎕ and 38㎕/50㎕ of culture droplet. (5) Culture medium containing heated fraction I and fraction III at optimum combination activated 51±6.11% of live spermatozoa after 5 hours incubation at 37℃. (6) Hamster ova incubated with activated hamster epididymal spermatozoa were fertilized by 23±4.12% after 4 hours incubation at 37℃. (7) From the results obtained in these experiments, it could be concluded that bovine seminal plasma included both sperm motility inhibiting and stimulation factor. The later may capacitate hamster spermatozoa in vitro.

동물산업에 있어서 생명공학 연구의 현황과 전망

정길생 건국대학교 동물자원연구센터 1990 국제 심포지움 Vol.- No.1

The nation wide demand for animal products has been daily increased by the change of overall dietary pattern with the rapid economic growth. In view of these circumstances, the import of animal products is inevitable to meet the increasing demand. However, opening the domestic market for agricultural products to other contries makes the domestic animal industry he faced with a critical moment, because its productivity is too low to compete with animal products from the foreign countries. Therefore, the best avenue to tide over this crisis is to enhance the productivity by developing and using the biotechniques. Biotechniques mainly related to animal production are summarized as following 1) collection of predominant genes, 2) increase of efficiencies of these genes, 3) manipulation of these genes, 4) preservation of these genes, 5) trasfer of embryo containing these genes, 6) control of sex, and 7) production and application of antibodies. At present, a few of above biotechniques are well developed and applied, but the others are at the beginning stage in the country. Furthermore, researches concerning these biotechniques are trying to imitate them of foreign countries are also not well established but just started. Accordingly, it could be possible for us to dominate these biotechniques in the world, if we make efforts to create the new ideas, to mike the heat of the technical information, and to educate technicians. But, it is impossible to accomplish these potential efforts without the financial support of government.

Prostaglandin F2α의 投與에 의한 돼지의 分娩誘起에 관한 硏究 - Ⅰ.投與方法이 分娩誘起에 미치는 影響

鄭吉生,延正雄 한국동물생명공학회(구 한국동물번식학회) 1979 Reproductive & developmental biology Vol.3 No.2