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Song, Yi,Jeong, Sung Woo,Lee, Won Sup,Park, Semin,Kim, Yun-Hi,Kim, Gon-Sup,Lee, Soo Jung,Jin, Jong Sung,Kim, Chi-Yeon,Lee, Ji Eun,Ok, Se Yun,Bark, Ki-Min,Shin, Sung Chul Hindawi Publishing Corporation 2014 Journal of analytical methods in chemistry Vol.2014 No.-
<P>The Korean prostrate spurge <I>Euphorbia supina</I> is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (<I>r</I><SUP>2</SUP>) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1–102.8% and 85.2–98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.</P>
Cloning and Expression of Pseudomonas cepacia catB Gene in Pseudomonas putida
Song, Seung-Yeon,Jung, Young-Hee,Lee, Myeong-Sok,Lee, Ki-Sung,Kim, Young-Soo,Kim, Chi-Kyung,Choi, Sang-Ho,Min, Kyung-Hee The Microbiological Society of Korea 1996 The journal of microbiology Vol.34 No.4
The enzyme, cis,cis-muconate lactonizing enzyme has been proposed to play a key role in the $\beta$-ketoadipate pathway of benzoate degradation. A 3.2-kb EcoRI fragment termed as pRSU2, isolated from a Pseudomonas cepacia genomic library was able to complement the catB defective mutant. Several relevant restriction enzyme sites were determined within the cloned fragment. In Pseudomonas putida SUC2 carrying pRSU2, the enzyme activity was relatively higher than those of the induced or partially induced state of wild type P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida. One possible interpretation of these results is that the catB promoter in P. cepacia is recognized within P. putida, resulting in the almost same expression level.
Effects of Essential Amino Acids on the In Vitro Development of Porcine Parthenogenetic Embryos
Yeon-Ik Jeong,Chi-Hun Park,Dong-Kyung Lee,Hee-Jin Song,Yeun-Wook Kim,Yeon-Woo Jeong,Sang-Hwan Hyun,Hwan-Yul Yong,Woo-Suk Hwang 한국동물생명공학회(구 한국동물번식학회) 2013 Reproductive & Developmental Biology(Supplement) Vol.37 No.2s
Amino acids have been regarded as beneficial for the development of preimplantation embryos and acted critically in the in vitro culture of porcine embryos. However, some reviews of emerging literatures show that supplementation of essential amino acids (EAAs) to in vitro culture medium was improved less than usually expected. The present study was designed to investigate the effects of EAAs on the in vitro development of porcine preimplantation embryos after parthenogenetic activation. The results showed that supplementation of EAAs to porcine zygote medium-3 (PZM-3) with 1:50 significantly decreased the blastocyst rate of parthenogenetic embryos (12.8±4.0 vs. 25.6±4.2) although cleavage rate was not different (p<0.05). On the other hand, the distribution pattern of total cell number of the blastocysts varied widely while the average number has not shown significant difference. Real-time qPCR analysis revealed that EAAs have turned over transcription pattern of imprinted genes: the relative abundance of H19 transcript was enhanced and level of IGF2r transcript was decreased significantly (p<0.05). Interestingly, gene expression of mTOR, activated by some essential amino acids, was significantly up-regulated (p<0.05). In conclusion, these results indicate that the presence of essential amino acids in the in vitro culture medium might impair embryo development due to mRNA turnover of imprinted genes, but could possibly play an important role at the implantation stage because development of protrusive trophoblast activity requires some amino acids-dependent mTOR signaling in early blastocysts.
Song, Seung Yeon,Han, Yong Duk,Park, Yoo Min,Jeong, Chi Yong,Yang, Yong Ju,Kim, Moo Sub,Ku, Yunhee,Yoon, Hyun C. Elsevier 2012 Biosensors & bioelectronics Vol.35 No.1
<P><B>Abstract</B></P> <P>We developed an electrochemical glycated hemoglobin (HbA<SUB>1c</SUB>) biosensor for diagnosing diabetes in whole human blood based on the competitive binding reaction of glycated proteins. Until now, no studies have reported a simple and accurate electrochemical biosensor for the quantification of HbA<SUB>1c</SUB> in whole blood. This is because it is very difficult to correctly distinguish HbA<SUB>1c</SUB> from large amounts of hemoglobin and other components in whole blood. To detect glycated hemoglobin, we used electrodes modified with boronic acid, which forms a covalent bond between its diol group and the cis–diol group of the carbohydrate moiety of glycated proteins. For accurate HbA<SUB>1c</SUB> biosensing, we first removed blood components (except for hemoglobin) such as glycated proteins and blood glucose as they interfere with the boronate-based HbA<SUB>1c</SUB> competition analysis by reacting with the boronate-modified surface via a cis–diol interaction. After hemoglobin separation, target HbA<SUB>1c</SUB> and GOx at a predetermined concentration were reacted through a competition onto the boronate-modified electrode, allowing HbA<SUB>1c</SUB> to be detected linearly within a range of 4.5–15% of the separated hemoglobin sample (HbA<SUB>1c</SUB>/total hemoglobin). This range covers the required clinical reference range of diabetes mellitus. Hence, the proposed method can be used for measuring %HbA<SUB>1c</SUB> in whole human blood, and can also be applied to measuring the concentration of various glycated proteins that contain peripheral sugar groups.</P> <P><B>Highlights</B></P> <P>▸ We developed an accurate electrochemical HbA<SUB>1c</SUB> biosensor in whole human blood based on the competitive binding reaction of glycated proteins. ▸ Hemoglobin was separated from other blood components using a zinc ion-mediated separation method. ▸ Target HbA<SUB>1c</SUB> and signaling GOx was bound competitively onto the boronate group-modified electrode. ▸ HbA<SUB>1c</SUB> concentrations were determined linearly within a range of 4.5–15%, covering the required clinical reference range.</P>
다중압출공정을 이용하여 제조한 섬유상 HAp-(t-ZrO2)/Al2O3-(m-ZrO2) 복합소결체의 미세조직과 기계적특성
이치우 ( Chi Woo Lee ),윤민호 ( Min Ho Youn ),송호연 ( Ho Yeon Song ),이병택 ( Byong Taek Lee ) 대한금속재료학회 ( 구 대한금속학회 ) 2006 대한금속·재료학회지 Vol.44 No.6
Core-shell structured HAp-(t-ZrO2)/Al2O3-(m-ZrO2) composites were fabricated using a multi extrusion process. The shell of Al2O3-(m-ZrO2) phases was selected due to their excellent biocompatibility and mechanical properties and the core was designed with t-ZrO2 dispersed HAp matrix, In the 3rd passed sample, core/shell and fibrous microstructures having 4㎛ in diameter and 1㎛ in thickness were clearly observed. In the composite sintered at 1200℃, many pores were observed in both HAp-(t-ZrO2) core and Al2O3-(m-ZrO2) shell regions. However at 1500℃, the shell regions showed dense microstructure. The values of relative density, hardness, bending strength and fracture toughness in the sample sintered at 1500℃ were higher than those of 1200℃ and their maximum values were at 1500℃ about 95%, 967Hv, 278 MPa and 4.2 MPam(1/2), respectively. The fracture morphology was appeared with homogeneously rough surface due to the crack deflection and phase transformation of t-ZrO2 particles.
The indices of body size and aggressiveness of papillary thyroid carcinoma
Ju-Yeon Kim,Eun-Jung Jung,Sang-Ho Jeong,Chi-Young Jeong,Young-Tae Ju,Young-Jun Lee,Soon-Chan Hong,Sang-Kyeong Choi,Woo-Song Ha,Soon-Tae Park 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.80 No.4
Purpose: The incidence of papillary thyroid carcinoma (PTC) is increasing worldwide. Positive associations between indices of body size and thyroid cancer have been reported. However, the relationships to cancer severities and/or behaviors are uncertain. Methods: We performed a retrospective analysis of the data of patients who underwent total thyroidectomy due to PTC. The epidemiologic factor and pathologic report after operation were determined based on chart review. The relationships between indices of body size and these parameters were assessed. Results: Positive association between body mass index and T stage was found, but it was not statically significant. In neck lymph node metastasis, the group with metastasis had a tendency for larger mean height and weight, but significant difference was found only in height. However, in the multivariate analysis, the age and size of nodules were only identified as independent risk factors of neck lymph node metastasis (P = 0.000 and 0.019). Conclusion: There was no independent association between indices of body size and stages of PTC in patients who underwent total thyroidectomy.
Initiation of Guideline-Matched Oral Anticoagulant in Atrial Fibrillation-Related Stroke
Mi-Yeon Eun,Jae-Young Kim,황양하,Man-Seok Park,Joon-Tae Kim,Kang-Ho Choi,Jin-Man Jung,Sungwook Yu,Chi Kyung Kim,Kyungmi Oh,Tae-Jin Song,Yong-Jae Kim,Bum Joon Kim,허성혁,Kwang-Yeol Park,Jeong-Min Kim,Jong-Ho 대한뇌졸중학회 2021 Journal of stroke Vol.23 No.1
Background and Purpose: To evaluate the outcome events and bleeding complications of the European Society of Cardiology (ESC) guideline-matched oral anticoagulant therapy for patients with acute ischemic stroke and atrial fibrillation (AF). Methods: Patients with acute ischemic stroke and AF from a nationwide multicenter registry(Korean ATrial fibrillaTion EvaluatioN regisTry in Ischemic strOke patieNts [K-ATTENTION]) between January 2013 and December 2015 were included in the study. Patients were divided into the ESC guideline- matched and the non-matched groups. The primary outcome was recurrence of any stroke during the 90-day follow-up period. Secondary outcomes were major adverse cerebrovascular and cardiovascular events, ischemic stroke, intracranial hemorrhage, acute coronary syndrome, allcause mortality, and major hemorrhage. Propensity score matching and logistic regression analyses were performed to assess the effect of the treatments administered. Results: Among 2,321 eligible patients, 1,126 patients were 1:1 matched to the ESC guidelinematched and the non-matched groups. As compared with the non-matched group, the ESC guideline- matched group had a lower risk of any recurrent stroke (1.4% vs. 3.4%; odds ratio[OR], 0.41; 95% confidence interval [CI], 0.18 to 0.95). The risk of recurrent ischemic stroke was lower in the ESC guideline-matched group than in the non-matched group (0.9% vs. 2.7%; OR, 0.32; 95% CI, 0.11 to 0.88). There was no significant difference in the other secondary outcomes between the two groups. Conclusions: ESC guideline-matched oral anticoagulant therapy was associated with reduced risks of any stroke and ischemic stroke as compared with the non-matched therapy.