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Symposium 7 : Plant Biochemistry ; Identification of rice genes with Ac/Ds mediated gene trap system
한창덕 ( Chang Deok Han ),( Byoun Il Je ),( Su Hyun Park ),( Chul Min Kim ),( Sung Han Park ),( Yun Jeong Na ),( Jung Ja Lee ),( Byeong Geun Oh ),( Nam Min Hee ),( Gi Hwan Yi ),( Ho Yong Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2000 추계학술대회집 Vol.2000 No.-
원용재,이기환,조준현,고종민,박향미,한창덕,양세준,김순철,남민희,Won, Yong-Jae,Yi, Gi-Hwan,Cho, Jun-Hyeon,Ko, Jong-Min,Park, Hyang-Mi,Han, Chang-Deok,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1
This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y. 형질전환 식물체를 직접 이용하기에는 후대에 유전적인 고정 및 포장검정이라는 문제가 대두되는데, 이를 해결하기 위하여 본 연구는 바스타저항성 유전자가 도입된 벼와 직파적 응성 계통을 교잡한 후 약배양에 의한 형질전환체 고정 등 기존 육종체계와 연계하여 제초제 저항성 직파적응 계통을 조기에 육성할 수 있는 체계를 확립하고자 수행하였다. 제초제 저항성 직파적응 계통을 조기에 육성하고자 1999년 동계에 교배된 동진벼 Ds15(Ba $r^{R}$)/주남벼 등 5조합으로부터 2000년 $F_1$을 양성하고, 이들을 PCR분석으로 제초제 저항성 유전자 bar도입을 확인한 후 약배양을 실시하여 얻어진 1,508개의 재분화 식물체 중 제초제 저항성 유전자가 도입된 169개체 ( $A_2$)에서 채종하였다. 이들을 2001년 포장에 공시하여 42계통을 선발하고,2002년에 동진벼 Ds15 (Ba $r^{R}$)/주남벼 조합에서 수량 등 주요 농업형질이 우수한 7계통을 생산력 검정시험에 공시한 결과 제초제 저항성이면서 직파적응성 등 기타 농업형질이 우수한 YR23235Acp79를 선발하여 밀양 204호로 계통명을 부여하였다. 결과적으로 형질전환체를 이용하여 교배부터 계통선발까지 3년 만에 목적하는 계통을 조기에 육성할 수 있는 생력육종체계가 확립되었다.
정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희,Jeong Eung-Gi,Yi Gi-Hwan,Won Yong-Jea,Park Hyang-Mi,Cheon Nam-Soo,Choi Jun-Ho,Ku Yeon-Chung,Han Chang-Deok,Eun Mu-Yeong,Kim Tae-Sas,Nam Min-Hee 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2
밀양 204호는 작물과학원 영남농업연구소 생명공학연구팀이 직파적응 제초제저항성 벼 품종을 육성할 목적으로 '98년 bar 유전자를 동진벼에 아그로박테리움법으로 형질 전환하여 제초제저항성 벼를 양성하였다. '98/'99년 동계에 단간 내도복 양질인 주남벼를 인공교배하여 약배양 등 육종프로그램에 적용하여 우량계통을 선발하고 밀양 204호로 계통명을 부여하였다. GM 벼와 Non-GM 벼의 농업적 특성을 UPOV 및 국립종자관리소의 품종등록 기준에 따른 농업적특성 중 차이가 있었던 것은 엽색도, 지엽의 형태, 간장, 수당립수 등 이었다. This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.
이강섭,박성한,윤도원,안병옥,김창국,한창덕,이기환,박동수,은무영,윤웅한,Lee, Gang-Seob,Park, Sung-Han,Yun, Do-Won,Ahn, Byoung-Ohg,Kim, Chang-Kug,Han, Chang-Deok,Yi, Gi-Hwan,Park, Dong-Soo,Eun, Moo-Young,Yoon, Ung-Han 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2
Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.
Small non-coding RNA를 발현하는 형질전환 벼의 환경위해성 평가 방법
진병준,전현진,조현민,이수현,최철우,정욱헌,백동원,한창덕,김민철,Jin, Byung Jun,Chun, Hyun Jin,Cho, Hyun Min,Lee, Su Hyeon,Choi, Cheol Woo,Jung, Wook-Hun,Baek, Dongwon,Han, Chang-deok,Kim, Min Chul 한국식물생명공학회 2019 식물생명공학회지 Vol.46 No.3
Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNA-mediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.
정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
This study was conducted to investigated the major characteristics of genetically modified rice of "Milyang 204" originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Junambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of "Milyang 204" showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic "Milyang 204" showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the nontransgenic varieties rice.