단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

The changes in the proteolysis activity and the accumulation of free amino acids during chinese traditional dry-cured loins processing

Chang-Yu Zhou,Ying Wang,Dao-Dong Pan,Jin-Xuan Cao,Yin-Ji Chen,Yuan Liu,Yang-Ying Sun,Chang-Rong Ou 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3

Twelve pieces of longissimus dorsi were processed into Chinese traditional dry-cured loins. The changes in the proteolylic enzymes activities, myofibrillar proteins degradation, and free amino acids content were investigated during processing. Compared with fresh piece (0 day), the cathepsin B ? L and calpains activities decreased after dry-curing and maintained potential activities values of 23.25 and 15.04% in the final products, respectively. The myosin heavy chain (MHC) and C protein were intensely degraded at the dry-ripened stage; the 50 kDa desmin increased at day 2 and then disappeared at day 11. The total free amino acids content increased from 333.18 mg/100 g in the raw to 1096.54 mg/100 g at the end of the dry-ripening. This work provided a mechanism for the accumulation of free amino acids and predicted the proteolysis extent of myofibrillar proteins by monitoring the changes of three marker proteins (MHC, C protein and 50 kDa desmin) during Chinese traditional dry-cured loins processing. Keywords

Monitoring changes in the genetic structure of Brown Tsaiya duck selected for feeding efficiency by microsatellite markers

Yi-Ying Chang,Hsiu-Chou Liu,Chih-Feng Chen Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.3

Objective: Few studies have genetically monitored chickens over time, and no research has been conducted on ducks. To ensure the sustainable management of key duck breeds, we used microsatellite markers to monitor Brown Tsaiya ducks over time genetically. Methods: The second, fourth, sixth to eighth generations of the Brown Tsaiya duck selected for feeding efficiency and control lines were included in this study to investigate the genetic variations, effective population size, population structure and the differentiation between populations over time with 11 microsatellite markers derived from Brown Tsaiya duck. Results: The results showed there were a slight decrease in the genetic variations and an increase in within-population inbreeding coefficient (F_IS) in both lines, but no consistent increase in F_IS was observed in each line. The effective population size in the second and eighth generations was 27.2 for the selected line and 23.9 for the control line. The change in allele richness showed a downward trend over time, and the selected line was slightly lower than the control line in each generation. The number of private alleles (N_p) in the selected line were higher than in the control line. Moderate differentiation was observed between the second and eighth generations in the selected line (F_ST = 0.0510) and the control line (F_ST = 0.0606). Overall, differentiation tended to increase with each generation, but genetic variation and structure did not change considerably after six generations in the two lines. Conclusion: This study provides a reference for poultry conservation and helps to implement cross-generation genetic monitoring and breeding plans in other duck breeds or lines to promote sustainable management.

Drilling Temperature and Hole Quality in Drilling of CFRP/Aluminum Stacks Using Diamond Coated Drill

Chang-Ying Wang,Yu-Han Chen,Qinglong An,Xiaojiang Cai,Wei-Wei Ming,Ming Chen 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Composite/metal stack materials, most often consisting of carbon fiber reinforced polymer (CFRP) composites and aluminum or titanium alloy (Al or Ti), have been widely used in the aviation industry. It is still a challenge to make holes on the composite/metal stack materials with high quality. This paper aims to investigate the influence of drilling parameters on the drilling of stack materials consisting of T800/X850 CFRP and 7075-T651 Al with regard to drilling force, drilling temperature, hole diameter and hole surface quality. Diamond coated drill bits with double point angles were employed in the drilling tests. The drilling temperature was measured utilizing a rotational motion temperature measuring system. It is indicated that maximum drilling temperature increases with the increase of spindle speed, while, it decreases with increasing feed rate. The thrust force and drilling temperature increase abnormally during drilling of the Al layer, which can be attributed to the evacuation difficulty of the long and flexible Al chips. It is found that sudden drops in the surface profile of CFRP are caused by the cavities owning to the resin degradation effects. The influences of different stack sequences on the drilling temperature and hole surface quality have also been discussed.

Blocking Artifacts Reduction using Two Modes Shift Block Filter

Ying-Wen Chang,Yen-Yu Chen 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7

Blocking effect is the major drawbacks in DCT-based codec at low bit-rate. An efficient anti-blocking effect algorithm is induced in the DCT domain. The proposed algorithm eases the false edges with low computational complexity. Two operations are suggested to different region according the region feature. One operation applies to the smooth region and the boundary edges become to linear distribution and more gradual. The other operation tries to remove some high frequency DCT component and to make the false edge smoothness but not harm for the image quality. The proposed scheme can take best quality in both objective and subjective metrics. Moreover, the objective metric, Peak Signal-to-Noise Ratio (PSNR), can improve to 0.5 ㏈.

Re-Thinking the Meaning of Home- A Critical Review of Government Supported Housing Policy in China

Ying Chang 한국주거학회 2015 한국주거학회 국제학술대회논문집 Vol.2015 No.4

The Optical Measurement and Quantitative Analysis of Algesia in Spodoptera litura Larva

Ying-Yun Chen,Rong-Seng Chang,Mi-Yin Tsai,Der-Chin Chen 한국광학회 2015 Current Optics and Photonics Vol.19 No.2

Muscle vibration measurement has long been an unique scientific study, in general, and the directreaction of animals to feel pain (algesia), either from vascular or muscle contraction, is a complexperceptual experience. Thus this paper proposes a way to measure animal algesia quantitatively, bymeasuring the changes in muscle vibration due to a pinprick on the surface of the skin of a Spodopteralitura larva. Using the laser optical triangulation measurement principle, along with a CMOS image sensor,linear laser, software analysis, and other tools, we quantify the subtle object point displacement, with aprecision of up to 10 μm, for our chosen Spodoptera litura larva animal model, in which it is not easyto identify the tiny changes in muscle contraction dynamics with the naked eye. We inject differentconcentrations of formalin reagent (empty needle, 12% formalin, and 37% formalin) to obtain a varietyof different muscle vibration frequencies as the experimental results. Because of the high concentrationsof reagent applied, we see a high frequency shift of muscle vibration, which can be presented as painindices, so that the algesia can be quantified

Five-Year Follow-Up Results of a Randomized Controlled Trial Comparing Bipolar Plasmakinetic and Monopolar Transurethral Resection of the Prostate

Chang-Ying Xie,Xing-Huan Wang,Guang-Bin Zhu,Xiang-Bin Liu 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.4

Purpose: To report the 5-year follow-up results of a randomized controlled trial comparing bipolar transurethral resection of the prostate (TURP) with standard monopolar TURP for the treatment of benign prostatic obstruction (BPO). Materials and Methods: A total of 220 patients were randomized to bipolar plasmakinetic TURP (PK-TURP) or monopolar TURP (M-TURP). Catheterization time was the primary endpoint of this study. Secondary outcomes included operation time, hospital stay, as well as decline in postoperative serum sodium and hemoglobin levels. All patients were assessed preoperatively and followed-up at 1, 6, 12, 24, 36, 48, and 60 months postoperatively. Parameters assessed included quality of life, transrectal ultrasound, serum prostate-specific antigen level, postvoid residual urine volume, maximum urinary flow rates (Qmax), and International Prostate Symptom Score. Patient baseline characteristics, perioperative data including complications, and postoperative outcomes were compared. Complication occurrence was graded according to the modified Clavien classification system. Results: PK-TURP was significantly superior to M-TURP in terms of operation time, intraoperative irrigation volume, resected tissue weight, decreases in hemoglobin and sodium, postoperative irrigation volume and time, catheterization time, and hospital stay. At 5 years postoperatively, efficacy was comparable between arms. No differences were detected in safety outcomes except that the clot retention rate was significantly greater after M-TURP. Conclusion: Our results indicate that PK-TURP is equally as effective in the treatment of BPO, but has a more favorable safety profile in comparison to M-TURP. The clinical efficacy of PK-TURP is long-lasting and comparable with M-TURP.

Progressive collapse analysis of steel frame structure based on the energy principle

Chang Hong Chen,Yan Fei Zhu,Yao Yao,Ying Huang 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.21 No.3

The progressive collapse potential of steel moment framed structures due to abrupt removal of a column is investigated based on the energy principle. Based on the changes of component's internal energy, this paper analyzes structural member's sensitivity to abrupt removal of a column to determine a sub-structure resisting progressive collapse. An energy-based structural damage index is defined to judge whether progressive collapse occurs in a structure. Then, a simplified beam damage model is proposed to analyze the energies absorbed and dissipated by structural beams at large deflections, and a simplified modified plastic hinges model is developed to consider catenary action in beams. In addition, the correlation between bending moment and axial force in a beam during the whole deformation development process is analyzed and modified, which shows good agreement with the experimental results.

Multi-Detector Row Computed Tomographic Evaluation of a Rare Type of Complete Vascular Ring: Double Aortic Arch with Atretic Left Arch Distal to the Origin of Left Subclavian Artery

Ying-Ying Hung,Yun-Ching Fu,Hao-Ji Wei,I-Chen Tsai,Clayton Chi-Chang Chen 대한영상의학회 2013 Korean Journal of Radiology Vol.14 No.5

Double aortic arch with an atretic left arch distal to the origin of left subclavian artery was diagnosed with multidetector row computed tomography (MDCT) in two children with dysphagia. This rare type of complete vascular ring is clinically important because it may be confused with right aortic arch in mirror imaging. Anatomic details of this rare type of complete vascular ring demonstrated on MDCT facilitated appropriate surgical treatment.