단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

The changes in the proteolysis activity and the accumulation of free amino acids during chinese traditional dry-cured loins processing

Chang-Yu Zhou,Ying Wang,Dao-Dong Pan,Jin-Xuan Cao,Yin-Ji Chen,Yuan Liu,Yang-Ying Sun,Chang-Rong Ou 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3

Twelve pieces of longissimus dorsi were processed into Chinese traditional dry-cured loins. The changes in the proteolylic enzymes activities, myofibrillar proteins degradation, and free amino acids content were investigated during processing. Compared with fresh piece (0 day), the cathepsin B ? L and calpains activities decreased after dry-curing and maintained potential activities values of 23.25 and 15.04% in the final products, respectively. The myosin heavy chain (MHC) and C protein were intensely degraded at the dry-ripened stage; the 50 kDa desmin increased at day 2 and then disappeared at day 11. The total free amino acids content increased from 333.18 mg/100 g in the raw to 1096.54 mg/100 g at the end of the dry-ripening. This work provided a mechanism for the accumulation of free amino acids and predicted the proteolysis extent of myofibrillar proteins by monitoring the changes of three marker proteins (MHC, C protein and 50 kDa desmin) during Chinese traditional dry-cured loins processing. Keywords

Monitoring changes in the genetic structure of Brown Tsaiya duck selected for feeding efficiency by microsatellite markers

Yi-Ying Chang,Hsiu-Chou Liu,Chih-Feng Chen Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.3

Objective: Few studies have genetically monitored chickens over time, and no research has been conducted on ducks. To ensure the sustainable management of key duck breeds, we used microsatellite markers to monitor Brown Tsaiya ducks over time genetically. Methods: The second, fourth, sixth to eighth generations of the Brown Tsaiya duck selected for feeding efficiency and control lines were included in this study to investigate the genetic variations, effective population size, population structure and the differentiation between populations over time with 11 microsatellite markers derived from Brown Tsaiya duck. Results: The results showed there were a slight decrease in the genetic variations and an increase in within-population inbreeding coefficient (F_IS) in both lines, but no consistent increase in F_IS was observed in each line. The effective population size in the second and eighth generations was 27.2 for the selected line and 23.9 for the control line. The change in allele richness showed a downward trend over time, and the selected line was slightly lower than the control line in each generation. The number of private alleles (N_p) in the selected line were higher than in the control line. Moderate differentiation was observed between the second and eighth generations in the selected line (F_ST = 0.0510) and the control line (F_ST = 0.0606). Overall, differentiation tended to increase with each generation, but genetic variation and structure did not change considerably after six generations in the two lines. Conclusion: This study provides a reference for poultry conservation and helps to implement cross-generation genetic monitoring and breeding plans in other duck breeds or lines to promote sustainable management.

Study on the Interaction of Compound Bonded Magnets

Ying Chang,Nuo Ma,Xiaojun Yu,Fazeng Lian,Wei Li 한국자기학회 2004 Journal of Magnetics Vol.9 No.4

The attempt for the addition of double-phase nanocomposite Nd₂Fe₁₄B/Fe₃B powders, respectively, into several RE₂Fe₁₄B (RE=Pr, Nd) powders with high magnetic properties was carried out. The powders were compounded and compressed to take shape bonded magnets. By means of investigating the variation of compound magnet Br, the interaction between magnetic powders was revealed. The result shows that not chemical just but physical interaction exists between elements. The compound effect of Nd₂Fe₁₄B/Fe₃B-ferrite bonded magnets was detailed studied. The functional relation was revealed between magnetic properties and ferrite content. That is Y = 5.42 x²-11.34 x + 6.62. The variation of iHc temperature coefficient β_(iHc) with ferrite content was investigated. Following the ferrite content increased, β_(iHc) and hirr were obviously decreased, compression-resistant strength was enhanced.

Blocking Artifacts Reduction using Two Modes Shift Block Filter

Ying-Wen Chang,Yen-Yu Chen 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7

Blocking effect is the major drawbacks in DCT-based codec at low bit-rate. An efficient anti-blocking effect algorithm is induced in the DCT domain. The proposed algorithm eases the false edges with low computational complexity. Two operations are suggested to different region according the region feature. One operation applies to the smooth region and the boundary edges become to linear distribution and more gradual. The other operation tries to remove some high frequency DCT component and to make the false edge smoothness but not harm for the image quality. The proposed scheme can take best quality in both objective and subjective metrics. Moreover, the objective metric, Peak Signal-to-Noise Ratio (PSNR), can improve to 0.5 ㏈.

Re-Thinking the Meaning of Home- A Critical Review of Government Supported Housing Policy in China

Ying Chang 한국주거학회 2015 한국주거학회 국제학술대회논문집 Vol.2015 No.4

Response of Growth Performance, Cecal Fermentation Traits and In vitro Gas Production to Substitution of Soyhulls for Lignified Fiber in Rabbit Diets

Chang, Ying,Qin, Yinghe,Xiong, Yiqiang,Du, Yuchuan,Meng, Qingxiang Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.1

A growth trial (Expt. 1) and an in vitro fermentation experiment (Expt. 2) were conducted to determine the response of growth performance, cecal fermentation characteristics and in vitro gas production to incremental levels of substitution of digestible fiber for lignified fiber in the diet of weaned rabbits. Three diets, formulated by substituting soyhulls (SH; used as digestible fiber source) for soybean straw (used as lignified fiber source) at substitution levels of 0, 25 and 50%, were used in a factorial design. In the growth trial (Expt. 1), increasing levels of SH substitution resulted in a quadratic increase in daily body weight gain rate (p<0.04) and feed conversion efficiency (p<0.02), but in a numerical decrease in dietary DM intake (p=0.15). When SH were included in the diet at 25% substitution level, rabbits had the highest rate of liveweight gain and feed conversion efficiency. As SH substitution level increased, pH values and ammonia-N of cecal contents linearly (p<0.001) decreased, but total VFA concentration linearly (p<0.03) increased. With incremental levels of SH substitution, the percentage of acetate and butyrate linearly (p<0.05) reduced, but the percentage of propionate and minor acids linearly (p<0.03) increased. Increasing the SH substitution levels tended to increase incidence of diarrhea. In the in vitro fermentation experiment (Expt. 2), regardless of origin of substrates fermented, increasing SH substitution level resulted in increased maximal gas production (p<0.001) and shortened gas production lag time, but had no effect on gas production rate (p>0.2). These observations suggest that incrementally feeding SH to rabbits could stimulate their cecal microbial activity, allowing cecal fermentation to shift towards favoring fiber digestion. In conclusion, digestible fiber from soyhulls may partially substitute for more lignified fiber, soybean straw, without having an adverse effect on cecal fermentative and microbial activity and growth performance. For growing rabbits, about 73% of total dietary NDF should be supplied by effective NDF, the remainder could come from digestible NDF, such as soyhulls.

Drilling Temperature and Hole Quality in Drilling of CFRP/Aluminum Stacks Using Diamond Coated Drill

Chang-Ying Wang,Yu-Han Chen,Qinglong An,Xiaojiang Cai,Wei-Wei Ming,Ming Chen 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Composite/metal stack materials, most often consisting of carbon fiber reinforced polymer (CFRP) composites and aluminum or titanium alloy (Al or Ti), have been widely used in the aviation industry. It is still a challenge to make holes on the composite/metal stack materials with high quality. This paper aims to investigate the influence of drilling parameters on the drilling of stack materials consisting of T800/X850 CFRP and 7075-T651 Al with regard to drilling force, drilling temperature, hole diameter and hole surface quality. Diamond coated drill bits with double point angles were employed in the drilling tests. The drilling temperature was measured utilizing a rotational motion temperature measuring system. It is indicated that maximum drilling temperature increases with the increase of spindle speed, while, it decreases with increasing feed rate. The thrust force and drilling temperature increase abnormally during drilling of the Al layer, which can be attributed to the evacuation difficulty of the long and flexible Al chips. It is found that sudden drops in the surface profile of CFRP are caused by the cavities owning to the resin degradation effects. The influences of different stack sequences on the drilling temperature and hole surface quality have also been discussed.

The Optical Measurement and Quantitative Analysis of Algesia in Spodoptera litura Larva

Ying-Yun Chen,Rong-Seng Chang,Mi-Yin Tsai,Der-Chin Chen 한국광학회 2015 Current Optics and Photonics Vol.19 No.2

Muscle vibration measurement has long been an unique scientific study, in general, and the directreaction of animals to feel pain (algesia), either from vascular or muscle contraction, is a complexperceptual experience. Thus this paper proposes a way to measure animal algesia quantitatively, bymeasuring the changes in muscle vibration due to a pinprick on the surface of the skin of a Spodopteralitura larva. Using the laser optical triangulation measurement principle, along with a CMOS image sensor,linear laser, software analysis, and other tools, we quantify the subtle object point displacement, with aprecision of up to 10 μm, for our chosen Spodoptera litura larva animal model, in which it is not easyto identify the tiny changes in muscle contraction dynamics with the naked eye. We inject differentconcentrations of formalin reagent (empty needle, 12% formalin, and 37% formalin) to obtain a varietyof different muscle vibration frequencies as the experimental results. Because of the high concentrationsof reagent applied, we see a high frequency shift of muscle vibration, which can be presented as painindices, so that the algesia can be quantified

Study on the interaction of compound bonded magnets

Ying Chang,Fazeng Lian,Xiaojun Yu 한국자기학회 2003 한국자기학회 학술연구발표회 논문개요집 Vol.13 No.2