Characterization of changes in global gene expression in the brain of neuron-specific enolase/human Tau23 transgenic mice in response to overexpression of Tau protein.

Woo, Jong-Min,Park, So Jung,Kang, Ho Il,Kim, Byoung Guk,Shim, Sun Bo,Jee, Seung Wan,Lee, Su Hae,Sin, Ji Soon,Bae, Chang Joon,Jang, Mee Kyung,Cho, Chunghee,Hwang, Dae Youn,Kim, Chuel Kyu D.A. Spandidos 2010 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.25 No.5

<P>Tau is a neuronal phosphoprotein responsible for the formation of the neurofibrillary tangles in Alzheimer's disease. To characterize the changes in global gene expression in the brain of transgenic mice that overexpress human Tau23 protein in response to the increase of Tau23 phosphorylation, total RNA extracted from the hippocampus of 12-month-old transgenic and wild-type mice was converted to cDNA, labeled with biotin and hybridized to oligonucleotide microarrays. The microarray results were confirmed by real-time RT-PCR and Western blotting method. It was determined that 43 genes were up-regulated and 8 genes were down-regulated by Tau23 in transgenic mice compared to controls, based on the arbitrary difference in the 2-fold change. Among the up-regulated transcripts, those encoding for transporter and oxidoreductase were dramatically over-represented, followed by those related to regulatory molecule, cytoskeletal protein, signaling molecule, and extracellular matrix protein. Genes encoding for transcription factor, regulatory molecule, miscellaneous function, and chaperone were significantly reduced in the down-regulated group. The major genes in the up-regulated categories included Ecrg4, Folr1, Defb11, Aqp1 and Soctdc1. The major genes in the down-regulated categories were Ncor1, Gpm6a, and Hspd1. These results indicate that the microarray analysis identifies several gene functional groups and individual genes that respond to a sustained increase in Tau23 phosphorylation levels in the brain of transgenic mice. In addition, the results suggest the microarray test is a useful tool for increased understanding of the role of Tau23 protein in regulating neurodegenerative disorders.</P>

급성 췌장염으로 발현된 낭성 변화를 이릉킨 종격동 부갑상선 선종 1예

이준호,최영식,박진홍,허갑도,윤설영,최소진,권태헌,이창렬,손성표,박영효 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.3

Hypercalcemia due to hyperparathyroidism is rarely associated with acute pancreatitis. But, the relationship between hypercalcemia and pancreatitis still remains controvesial. Ectopic parathyroid adenoma with cystic change is one of the rare causes of hyperparathyroidism, and is usually located in neck and mediastinum. We report a case of mediastinal parathyroid adenoma with cystic change associated with acute pancreatitis. A 54-year-old male presented with epigastric pain for 3 days. The serum calcium, phosphate, elastase were 16.8mg/dL, 1.1 mg/dL, 2772.0 ng/mL respectively and his parathyroid hormone level in serum was 651.84 pg/mL. Chest CT showed a mediastinal mass with well defined inhomogenously enhanced density located between SVC and aorta. The patient was diagnosed to hyperparathyroidism with acute pancreatitis due to mediastinal parathyroid adenoma with cystic change, and was surgically removed. The surgical biopsy showed parathyroid adenoma with cystic change. After operation his general condition was improved and serum calcium, phosphate, amylase, lipase level were normalized (J Kor Soc Endocrinol 13:473-479, 1998).

Chemical Properties and Nutrient Loadings of Rainwater during Farming Season

Min-Kyeong Kim,Seong-Chang Hong,Jong-Sik Lee,Goo-Buk Jung,Soon-Ik Kwon,Mi-Jin Chae,Sun-Gang Yun,Kyu-Ho So 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.5

Recently, special attention has been given to acid rain and its problem to environment such as acid precipitation and air pollution in East Asia. In the present study, rainwater samples were collected from Apr to Nov in 2012. The samples were chemically characterized for the assessment of emission sources. Suwon and Yeoju regions, typical agricultural areas in South Korea, were chosen for study sites. Ion composition and cation-affected neutralization were determined to evaluate the contribution of cations to the acidity of rainwater. Ion and electrical conductivity between the measured and the estimated showed high correlation. The cations observed in Suwon and Yeoju were Na<SUP>+</SUP> > NH4<SUP>+</SUP> > K<SUP>+</SUP> > Ca<SUP>2+</SUP> > Mg<SUP>2+</SUP> > H<SUP>+</SUP> and Na<SUP>+</SUP> > K<SUP>+</SUP> > NH4<SUP>+</SUP> > Ca<SUP>2+</SUP> > Mg<SUP>2+</SUP> = H<SUP>+</SUP>, respectively. The anions of all sites were SO4<SUP>2-</SUP> > NO3- > Cl<SUP>-</SUP>. While the amounts of sulfate, one of the major dissolved components of rainwater, were 77.6 and 75.6 ueq L<SUP>-1</SUP> in Suwon and Yeoju, the ones of NSS-SO4<SUP>2-</SUP> (Non-Sea Salt sulfate) were 83 and 82% in Suwon and Yeoju, respectively. The comparison of observed pH values (pHobs) with the theoretical pH values (pHthe) showed that the neutralization of rain water considerably went along during the study periods. The highest amounts of rainfall throughout the year in Suwon and Yeoju were 572.3 and 484.6 mm in July, and its corresponding nitrogen loadings in Suwon and Yeoju were 5.28 and 3.50 kg ha-1, respectively. The major ion contents for crop growth with SO4<SUP>2-</SUP>, Ca<SUP>2+</SUP>, K<SUP>+</SUP> and Mg<SUP>2+</SUP> were 51.7, 5.2, 11.8 and 1.8 kg ha<SUP>-1</SUP> in Suwon and 34.2, 4.0, 4.2 and 1.1 kg ha<SUP>-1</SUP> in Yeoju.

급성 췌장염으로 발현된 낭성 변화를 일으킨 종격동 부갑상선 선종 1예

권태현,박진흥,윤설영,이창렬,이준호,최소진,손성표,허갑도,최영식,박영효 성분도병원 1998 성분도병원논문집 Vol.4 No.-

Effect of epidural corticosteroid injection on magnetic resonance imaging findings

( Min Soo Kim ),( Tae Yoon Jeong ),( Yu Seon Cheong ),( Young Wook Jeon ),( So Young Lim ),( Seong Sik Kang ),( In Nam Kim ),( Tsong Bin Chang ),( Hyun Ho Seong ),( Byeong Mun Hwang ) 대한통증학회 2017 The Korean Journal of Pain Vol.30 No.4

Background: Magnetic resonance imaging (MRI) of the spine is the preferred diagnostic tool for pathologic conditions affecting the spine. However, in patients receiving epidural corticosteroid injection (ESI) for treatment of spinal diseases, there is a possibility of misreading of MR images because of air or fluid in the epidural space after the injection. Therefore, we defined the characteristics of abnormal changes in MRI findings following an ESI in patients with low back pain. Methods: We reviewed the medical records of 133 patients who underwent MRI of the lumbar spine within 7 days after ESI between 2006 and 2015.All patients were administered an ESI using a 22-gauge Tuohy needle at the lumbar spine through the interlaminar approach. The epidural space was identified by the loss of resistance technique with air. Results: The incidences of abnormal changes in MRI findings because of ESI were 54%, 31%, and 25% in patients who underwent MRI at approximately 24 h, and 2 and 3 days after ESI, respectively. Abnormal MRI findings included epidural air or fluid, needle tracks, and soft tissue changes. Epidural air, the most frequent abnormal finding (82%), was observed in 41% of patients who underwent MRI within 3 days after injection. Abnormal findings due to an ESI were not observed in MR images acquired 4 days after ESI or later. Conclusions: Pain physicians should consider the possibility of abnormal findings in MR images acquired after epidural injection using the interlaminar approach and the loss of resistance technique with air at the lumbar spine. (Korean J Pain 2017; 30: 281-6)

중등교사의 생활체육참여에 따른 직무만족에 관한 연구

소영호,이창민 한국학교체육학회 2002 한국학교 체육학회지 Vol.12 No.1

This study has compared and analyzed the influence of "Sports for All" participation on middle school teachers' job satisfaction, 6 middle schools from Seoul and Kyungki-do was selected. 256 respondents was selected by stratified cluster random sampling and questionnaire was made by fell-administration method. The collected data was processed through SPSS FOR WINDOW 9.0 using One-Way ANOVA, chi-square and factor. Also DUNCON's discriminant analysis was uses to verify the statistical significance. I deduced the following result by this study method and procedure. 1 There was no statistical difference between non participating teachers and "Sports for All" participating teachers' job satisfaction. 2. Participating in "Sports for All' as a group or individually did not show statistical significance for job satisfaction, 3. The factors, such as participate frequency, hours, expense did not show statistical significance on job satisfaction. But experience showed statistical significance.(p<.05)

아토피 피부염의 중증도에 작용하는 중요인자

장가연,조소연,강호정,함정희 梨花女子大學校 醫科大學 醫科學硏究所 1998 EMJ (Ewha medical journal) Vol.21 No.3

목적: 아토피 피부염의 중증도나 동반 알러지 질환 유무에 따른 임상 및 검사실 소견의 차이와 그 의의를 알아보고자 하였다. 방법: 1995년 8월부터 1996년 7월까지 1년동안 본원 피부과 외래를 방문한 환자를 대상으로 피부과 의사의 직접적인 면담, 신체검사, 세균배양검사, 단자 검사 및 면역학적 검사를 시행하였다. 결과: 1) 피부 건조증, 이개 균열, hyperlinear paim의 동반이 중증도에 따른 유의성을 보였다. 2) 땀, 음식, 흡입항원이 중증군에서 통계학적으로 유의하게 악화요인으로 작용하였다. 3) 아토피 피부염의 가족력 도압ㄴ율이 중증군에서 57.9%, 경증군에서 26.3%로 중증도에 따른 유의성을 보였다. 4) 호흡기 아토피 질환 유무에 따른 집먼지 진드기에 대한 피부단자검사 양성율이 통계학적인 유의성을 보였다. 5) 총 혈청 IgE가 중증군에서 938.3±601.8IU/mL, 경증군에서 526.7±352.1IU/mL로 통계학적인 유의성을 보였다.(p<0.05) 6) 호흡기 아토피 질환이 동반된 아토피 피부염환자의 혈청 IgE는 1025.8±713.2IU/mL, 호흡기 아토피 질환이 동반되지 않은 군은 403.3±273.4IU/mL 로 유의한 차이를 보였다(p<0.05). 7) 혈청 호산구 값은 중증군에서는 509.9±398.4/㎣, 경증군에서는 340.5±219.4/㎣로 중증군에 따른 유의성을 보였다(p<0.05). 결론: 1) 아토피 피부염의 임상적 중증도와 연관있는 것은 피부 건조증, 이개균열, hyperlinear palm 같은 피부 보조증상, 땀, 음식, 흡입항원같은 악화요인이며, 혈청 IgE 증가 및 혈중 호산구증가같은 거사실 소견이었다. 2) 호흡기 아토피 질환이 있는 아토피 피부염 환자에서 높은 혈청 IgE치와 집먼지 진드기에 대한 높은 단자 검사 양성율을 보였다. Objectives : This study was performed to investigate the clinical manifestations and labora-tory findings, and to see whether the severity of atopic dermatitis(AD) implies a relationship to the clinical and laboratory findings. Methods : Our study was designed by analyzing outpatients with AD via physical examinat-ion, questionnaires and laboratory investigations such as prick test to house dust mites, bacterial cultures, total IgE, IgA, IgG, IgG_4, IgM, and peripheral eosinophil count. Results : 1) Xerosis, ear fissuring and hyperlinear palms were related to the severity of AD(p<0.05). 2) Sweating, foods and aeroallrgen were related to the severity of AD(p<0.05). 3) The presence of family history of AD differed significantly(p<0.05) between the severe group(57.9%) & the mild group(26.3%). 4) The positivity of the prick test to Dermatophagoides pteronyssinus and D.farinae was found in 90.0% of respiratory group and in 30.0% of AD only(p<0.05%). 5) Serum IgE level was higher in the severe group(938.3±601.8 IU/mL) than the mild group(526.7±352.1IU/mL)(p<0.05). 6) Serum IgE level in AD patients with respiratory disease(1025.8±713.2IU/mL) was higher than AD only(403.3±273.4IU/mL)(p<0.05). 7) Peripheral eosinophil counts were higher in the severe group(509.9±389.4/㎣) than the mild group(340.5±219.4/㎣)(p<0.05). Conclusion : The factors related to severity of AD were xerosis, ear fissuring, hyperlinear palms, sweating, food, aeroallergen, serum IgE and peripheral eosinophil count. The AD pati-ents with respiratory allergic disease had higher IgE levels and higher positive rates of prick test with house dust mite.

관상동맥내 스텐트 시술시 Aspirin과 Cilostazol 2개월 병합요법

윤명호,탁승제,염철훈,장혁재,최소연,유상용,안성균,고종훈,신준한,김한수,최병일 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

마이크로그로브 형태에 따른 평활근 세포 운동 측정

유소영,박찬영,정석,김동익,이병붕,허세호,한동철,장준근 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.2

Purpose: The spreading, orientation, and chemotaxis the gradient of a chemoattractant of smooth muscle cells (SMCs) were studied on the micro-grooved substrata by the light, fluorescence and scanning electron microscopy. Method: Vertical-walled grooves were produced in silicon wafers by the micromachining technique. All grooves were 4~20㎛ deep and 10~80㎛ wide, SMCs were cultured on each microgroove and examined under stereo-microscope. Result: Cell clusters were markedly oriented by all the grooved substrata examined. Tim-lapse images acquired from CCD (Charge Coupled Device) showed that the grooves directed the migration of SMCs. There was no prominent difference in the migration speed of SMCs according to the grooves. All the cytoskeletal fibers were reoragnized in the same direction with grooves. Especially the alignments of microtubule and intermediate filaments were distinguished in the SMCs on the micro grooves. Conclusion: These results could be applied to the analysis of vascular restenosis and the development of artificial blood vessels.

항우울제 장기 투여에 의한 흰쥐 해마에서 PhosphoCREB의 발현 증가

김소양,한진희,이창욱,백인호 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 저자들은 paroxetine(selective serotonin reuptake inhibitor). desipramine(selective norepinephrine reuptake inhibitor), moclobemide(reversible monoamine oxidase inhibitor)의 항우울제를 흰쥐에 장기 투여한 후 해마에서 phosphoCREB의 발현정도를 측정하고 이를 항정신병약물, 항불안제 및 control을 장기 투여하였을 때의 발현정도와 비교해 보고자 하였다. 방 법 : 실험동물로는 체중 200∼300g의 Sprague-Dawley 수컷 흰쥐 240마리를 사용하였고, paroxetine 투여군, desipramine 투여군, moclobemide 투여군, haloperidol 투여군, lorazepam 투여군과 대조군으로 나누고 각각의 약물을 1일간, 3일간, 7일간, 14일간 각각 하루 한차례씩 복강내 주입을 하였다. 흰쥐는 마지막 투약 15분후에 대뇌를 적출하였고 면역조직화학법을 이용하여 해마에서 발현되는 phosphoCREB(+)신경원 수를 측정하였다. 결 과 : 항우울제인moclobemide 투여군에서 3일째부터 phosphoCREB의 발현을 증가시켜 7일째와 14일째 모두 증가되었고 paroxetine 투여군과 desipramine 투여군도 7일째와 14일째 증가를 나타내었다. 그러나 항우울제가 아닌 약물을 투여한 군(lorazepam 투여군과 haloperidol 투여군)에서는 대조군과 비교하였을 때 투여 기간에 따른 phosphoCREB의 증가는 없었다. 결 론 : 다양한 항우울제의 장기 투여는 항정신병약물과 항불안제의 장기 투여와 비교하면 모두 해마에서 phosphoCREB의 발현을 증가시킴을 알 수 있었다. 따라서 이는 다른 약제와 구별되는 항우울제만의 특이한 공통적 약물작용기전으로 생각된다. Objectives : This study was designed to assess the expression of phosphoCREB in rat hippocampus after chronic administration of various antidepressants in comparison with chronic administration of antipsychotic and antianxiety drugs. Methods : Male Sprague-Dawley rats(200-300g) were used for this experiment. The subjects were divided into 6 groups according to specific treatment agents(paroxetine, desipramine, moclobemide, haloperidol, lorazepam, vehicle) which were administered daily for 1day, 3day, 7day, and 14days by intraperitoneal injection respectively. Brains were removed 15 minutes after the last treatment. PhosphoCREB immunoreactivity was measured by phosphoCREB(+) cell counts in hippocampus of rats. Results : Expression of phosphoCREB was significantly increased from day 3 in moclobemide group, from day 7 in paroxetine and desipramine groups, and increased most significantly from day 14 in all antidepressant-administered groups, with no increase in other two groups(lorazepam and haloperidol groups) throughout the experiment and even after 14 days of treatment. Conclusion : These result suggest that increased expression of phosphoCREB after chronic administration of antidepressants, not of antipsychotic or antianxiety drugs, demonstrates pharmacolgical specificity of antidepressant treatment in rat hippocampus regardless of their receptor preference.