Improved production of phleichrome from the phytopathogenic fungus Cladosporium phlei using synthetic inducers and photodynamic ROS production by phleichrome

So, K.K.,Jo, I.S.,Chae, M.S.,Kim, J.M.,Chung, H.J.,Yang, M.S.,Kim, B.T.,Kim, J.K.,Choi, J.K.,Kim, D.H. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.119 No.3

Two different diketopiperazines, cyclo-(l-Pro-l-Leu) and cyclo-(l-Pro-l-Phe), which were isolated from the culture filtrate of Epichloe typhina and found to be inducers of phleichrome production, were chemically synthesized and evaluated for use in the improved production of phleichrome from wild-type and UV-mutagenized strains (M0035) of Cladosporium phlei. When supplemented with PDA and V8 juice agar media, both inducers showed significant increases in the production of phleichrome. Phleichrome production was increased in a dose-dependent manner up to a concentration of maximum yield for both inducers. No further significant induction was observed by supplementing inducers over the concentration of maximum yield. Among the two inducers, cyclo-(l-Pro-l-Phe) showed better inducing capability than cyclo-(l-Pro-l-Leu). The maximum yield was observed from the M0035 strain grown on V8 juice media supplemented with 150 μM cyclo-(l-Pro-l-Phe), which was estimated to be 232.6 mg of phleichrome per gram of mycelia and 10.2 mg of secreted phleichrome per 20 agar-plugs. Interestingly, growth inhibition was observed on V8 juice agar media with 100, 150, and 200 μM cyclo-(l-Pro-l-Phe) but not on PDA with the same amount of inducer, which suggests that the inhibitory effect might be through the overproduction of phleichrome rather than the toxic effect of the inducer itself. Superoxide production by purified phleichrome was dramatically stimulated upon illumination, thus demonstrating photodynamic production of superoxide in vitro by phleichrome.

Optoperforations of Retinal Blood Vessels in an Intact Porcine Eye by Using a Femtosecond Laser-assisted Microsurgery System

M. S. Sidhu,Suk-Yi Woo,Wan Kee Kim,Heung Soon Lee,J. S. Yahng,Kyu Jin Kim,Sae Chae Jeoung,Hyun Kyu Lee 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.6

Femtosecond (fs) laser microsurgery is a powerful method for its potential to treat various retinal diseases, as well as to study the sub-cellular functions, because of its capability to selectively ablate a specific target (in vitro and in vivo) with minimal damage to the surrounding tissues. Here, we develop an fs-laser based microsurgery system, along with ophthalmoscope-assisted dynamic optical imaging system, for retinopathy treatment. The system is equipped with a galvano-scanner and a real-time imaging system. The laser system used for the current study consists of a regenerative amplified Ti-sapphire (λ = 810 nm) laser, delivering a 150-fs pulse at a repetition rate of 1 kHz. The illumination light from a white light-emitting diode (LED) was optically filtered to be maximally absorbed by blood, which allowed us to effectively differentiate the blood vessels from the retina surface. The estimated threshold fluences for inner limiting membrane (ILM) ablation and the primary blood vessel wall perforations within an intact porcine eye were found to be 2.6 ± 0.2 J/cm^2 and 5.0 ± 0.1 J/cm^2, respectively. The present work should be an important step in ongoing exploration of the use of femtosecond lasers for the purpose of treating retinal blood vessels.

가압고온 Conditioning 을 이용한 Pelleting 가공이 육성돈의 생산성에 미치는 영향

김명곤,오상집,채병조,이준엽,이영철,김지훈,한인규 한국축산학회 1997 한국축산학회지 Vol.39 No.5

Four differently processed diets, mash (M), pellet (P), high shear conditioned pellet (HSCP) and high shear conditioned crumble (HSCC) were prepared to evaluate the processing effect on the growth performance and nutrients digestibility in growing pigs. 1. HSCC had the lowest density and HSCP had the highest pellet durability. 2. P-fed pigs had less feed intake compared to HSCC-fed goup but showed the highest ADG and thus the most efficient feed conversion compared to other groups(P$lt;0.05). 3. P and HSCP processing improved protein and fat digestibilities and energy digestibility of P diet was higher than that of M diet (P$lt;0.05). 4. The Number of aerobic bacteria in the diet were drastically (97-98.5%) diminished by the heat treatments like P, HSCP and HSCC processing.

Multicenter phase II study of docetaxel plus oxaliplatin combination chemotherapy in patients with advanced gastric cancer: Daegu Gyeongbuk Oncology Group

Kim, J G,Sohn, S K,Chae, Y S,Song, H S,Kwon, K-Y,Do, Y R,Kim, M K,Lee, K H,Hyun, M S,Ryoo, H M,Bae, S H,Park, K U,Lee, W S,Baek, J H,Chung, H Y,Yu, W Nature Publishing Group 2008 The British journal of cancer Vol.98 No.3

<P>The present study was conducted to evaluate the efficacy and safety of a combination regimen of docetaxel plus oxaliplatin in patients with advanced gastric cancer. Patients with previously untreated metastatic or recurrent, measurable gastric cancer received intravenous docetaxel 65 mg m<SUP>−2</SUP> plus oxaliplatin 120 mg m<SUP>−2</SUP> on day 1 based on a 3-week cycle. Forty-two patients were enrolled in the current study, among whom 39 were assessable for efficacy and all assessable for toxicity. One complete response and 18 partial responses were confirmed, giving an overall response rate of 45.2% (95% confidence interval (CI); 31.7–59.7%). At a median follow-up of 7.7 months, the median time to progression and median overall survival was 5.7 (95% CI; 4.3–7.2) months and 9.9 (95% CI; 7.8–12.0) months, respectively. Grade 3/4 neutropenia occurred in 11 patients (26.1%) and febrile neutropenia was observed in four patients (9.5%). The common non-haematologic toxicity was fatigue (grade 1/2, 61.9%) and nausea (grade 1/2, 47.7%). The combination of docetaxel and oxaliplatin was found to be well tolerated and effective in patients with advanced gastric cancer.</P>

Oxide-free Sb₂S₃ sensitized solar cells fabricated by spin and heat-treatment of Sb(III)(thioacetamide)₂Cl₃

You, M.S.,Lim, C.S.,Kwon, D.H.,Heo, J.H.,Im, S.H.,Chae, K.J. Elsevier Science 2015 ORGANIC ELECTRONICS Vol.21 No.-

Pure Sb<SUB>2</SUB>S<SUB>3</SUB> without oxide impurities was formed by thermal decomposition of Sb(thioacetamide: TA)<SUB>2</SUB>Cl<SUB>3</SUB> precursor. From the analysis of thermal properties of Sb(TA)<SUB>2</SUB>Cl<SUB>3</SUB>, we developed a spin-coating and heat-treatment process to form pure Sb<SUB>2</SUB>S<SUB>3</SUB> thin-films with controllable thickness due to the formation of insoluble Sb<SUB>2</SUB>S<SUB>3</SUB> by heat-treatment. Through the spin-coating and heat-treatment process, we could fabricate oxide-free Sb<SUB>2</SUB>S<SUB>3</SUB> planar type sensitized solar cell with 8.12mA/cm<SUP>2</SUP> of short circuit current density (J<SUB>sc</SUB>), 0.616V of open circuit voltage (V<SUB>oc</SUB>), 45.9% of fill factor (F.F), and overall power conversion efficiency (η) of 2.3% at 1 sun condition.

Identification of New Microsatellite DNAs in the Chromosomal DNA of the Korean Cattle (Hanwoo)

Kim, J.W.,Hong, J.M.,Lee, Y.S.,Chae, S.H.,Choi, C.B.,Choi, I.H.,Yeo, J.S. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.10

To isolate the microsatellites from the chromosomal DNA of the Korean cattle (Hanwoo) and to use those for the genetic selection, four bacteriophage genomic libraries containing the chromosomal DNA of six Hanwoo steers showing the differences in meat quality and quantity were used. Screening of the genomic libraries using $^{32}P-radiolabeled 5'-({CA})_{12}-3$nucleotide as a probe, resulted in isolation of about 3,000 positive candidate bacteriophage clones that contain $(CA)_n$-type dinucleotide microsatellites. After confirming the presence of microsatellite in each positive candidate clone by Southern blot analysis, the DNA fragments that include microsatellite and flanking sequences possessing less than 2 kb in size, were subcloned into plasmid vector. Results from the analysis of microsatellite length polymorphism, using twenty-two PCR primers designed from flanking region of each microsatellite DNA, demonstrated that 208 and 210 alleles of HW-YU-MS#3 were closely related to the economic traits such as marbling score, daily gain, backfat thickness and M. longissimus dorsi area in Hanwoo. Interestingly, HW-YU-MS#3 microsatellite was localized in bovine chromosome 17 on which QTLs related to regulation of the body fat content and muscle ypertrophy locus are previously known to exist. Taken together, the results from the present study suggest the possible use of the two alleles as a DNA marker related to economic trait to select the Hanwoo in the future.

Effect of Fungal Elicitor, Pluronic F-68 and Methylcellulose on Suspension Culture of Mentha piperita Cells

Kang, Y. M.,Oh, J. H.,Chung, I. S.,Lee, H. J.,Chae, Y. A. 경희대학교 유전공학연구소 1992 遺傳工學論文集 Vol.4 No.-

The effect of fungal elicitor, pluronic F-68 and methylcellulose on suspension culture of M. piperita cells was investigated using shake flasks. About two fold increase in oil production was observed in response to the treatment of fungal elicitor prepared from Rhodotorula rubra. Pluronic F-68 or methylcellulose at a low concentration enhanced peppermint cell growth at 100 rpm of agitation.

X-ray absorption spectroscopy investigations on electronic structure and luminescence properties of Eu:SnO₂-SnO nanocomposites

Sharma, A.,Varshney, M.,Shin, H.J.,Chae, K.H.,Won, S.O. Elsevier 2016 Current Applied Physics Vol.16 No.10

<P>Tin oxide nanostructures are nontoxic in nature and biocompatible. However, they exhibit weak emission characteristics that limit their applications in cellular and biology fields. Therefore, it is important to enhance luminescence properties in this oxide and establish a relationship between the local electronic structure and emission characteristics. In this report, we demonstrate significant advancement in the photoluminescence (PL) properties in the Eu incorporated SnO2-SnO nanocomposites. Eu: SnO2-SnO nanocomposites were synthesized by chemical precipitation method. X-ray diffraction (XRD), X-ray absorption near edge structure (XANES) and PL measurements were performed to investigate the structural, electronic structure and emission properties of the samples. Eu3+ ions, as confirmed by the Eu M-5,M-4-edge XANES studies, lead to defect formation and local electronic structural perturbation in the nanocomposites. The O K-edge and Sn M-5,M-4-edge XANES studies confirm a net decrease in the hybridization of O 2p and Sn 5s/5p states and formation of Sn defects, upon Eu doping. Significant enhancement in the Eu3+ related interband transitions (D-5(0)-F-7(J); J = 0,1,2,3,4) is achieved upon increasing the Eu concentrations, indicating synthesis of emission characteristic rich SnO2-SnO nanocomposites. (C) 2016 Elsevier B.V. All rights reserved.</P>

Natural convective heat transfer of heated packed beds

Lee, D.Y.,Chae, M.S.,Chung, B.J. Pergamon Press 2017 International communications in heat and mass tran Vol.88 No.-

<P>Natural convection heat transfer of heated packed bed was investigated. Experiments were performed for a single heated sphere buried in unheated packed beds varying its locations and for packed beds with all heated spheres varying the heights of packed beds from 0.02 m to 0.26 m. Mass transfer experiments using a copper electroplating system were performed based upon the analogy between heat and mass transfer. The diameter of sphere was 0.006 m, which corresponds to Ra-d of 1.8 x 10(7). For the single heated sphere cases, the measured results agreed well with the existing natural convection heat transfer correlations for packed beds and even with those for a single sphere in an open channel. For all heated sphere cases, the average heat transfers decrease with increasing packed bed heights.</P>

Robust PEGylated hyaluronic acid nanoparticles as the carrier of doxorubicin: Mineralization and its effect on tumor targetability in vivo

Han, H.S.,Lee, J.,Kim, H.R.,Chae, S.Y.,Kim, M.,Saravanakumar, G.,Yoon, H.Y.,You, D.G.,Ko, H.,Kim, K.,Kwon, I.C.,Park, J.C.,Park, J.H. Elsevier Science Publishers 2013 Journal of controlled release Vol.168 No.2

The in vivo stability and tumor targetability of self-assembled polymeric nanoparticles are crucial for effective drug delivery. In this study, to develop biostable nanoparticles with high tumor targetability, poly(ethylene glycol)-conjugated hyaluronic acid nanoparticles (PEG-HANPs) were mineralized through controlled deposition of inorganic calcium and phosphate ions on the nanoparticular shell via a sequential addition method. The resulting nanoparticles (M-PEG-HANPs) had a smaller size (153.7+/-4.5nm) than bare PEG-HANPs (265.1+/-9.5nm), implying that mineralization allows the formation of compact nanoparticles. Interestingly, when the mineralized nanoparticles were exposed to acidic buffer conditions (<pH6.5), their sizes increased rapidly due to dissolution of the inorganic minerals. Doxorubicin (DOX), chosen as the model anticancer drug, was effectively encapsulated into the bare and mineralized nanoparticles. For bare PEG-HANPs, DOX was released in a sustained manner and its release rate was not dependent on the pH of the solution. On the other hand, DOX release from M-PEG-HANPs was pH-dependent: i.e. DOX was slowly released from nanoparticles under physiological condition (pH7.4), whereas its release rates were much higher at mildly acidic environments (<pH6.5). From in vivo biodistribution study, it was found that M-PEG-HANPs could reach the tumor site more effectively than bare PEG-HANPs. The antitumor efficacy of DOX-loaded nanoparticles was evaluated after systemic administration into the tumor-bearing mice. Of the samples tested, the most effective antitumor efficacy was observed for DOX-loaded M-PEG-HANPs. Overall, these results suggest that M-PEG-HANPs could be a promising carrier for an anticancer drug.