한우 c-fos 유전자의 염기서열 및 발현분석

유성란,정행진,정기철,이준헌,조규완,최재관,나기준,상병찬 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.

Hepatoprotective effect of vitamin C on lithocholic acid-induced cholestatic liver injury in Gulo(-/-) mice

Yu, S.J.,Bae, S.,Kang, J.S.,Yoon, J.H.,Cho, E.J.,Lee, J.H.,Kim, Y.J.,Lee, W.J.,Kim, C.Y.,Lee, H.S. North-Holland ; Elsevier Science Ltd 2015 european journal of pharmacology Vol.762 No.-

<P>Prevention and restoration of hepatic fibrosis from chronic liver injury is essential for the treatment of patients with chronic liver diseases. Vitamin C is known to have hepatoprotective effects, but their underlying mechanisms are unclear, especially those associated with hepatic fibrosis. Here, we analyzed the impact of vitamin Con bile acid induced hepatocyte apoptosis in vitro and lithocholic acid (LCA) induced liver injury in vitamin C-insufficient Gulo(-/-) mice, which cannot synthesize vitamin C similarly to humans. When Huh BAT cells were treated with bile acid, apoptosis was induced by endoplasmic retiiculum stress related JNK activation but vitamin C attenuated bile acid induced hepatocyte apoprosis in vitro. In our in vivo experiments. LCA feeding increased plasma marker of cholestasis and resulted in more extensive liver damage and hepatic fibrosis by more prominent apoptotic cell death and recruiting more intrahepatic inflammatory CD11b(+) cells in the liver of vitamin C-insufficient Gulo(-/-) mice compared to wild type mice which have minimal hepatic fibrosis. However, when vitamin C was supplemented to vitamin C-insufficient Gulo(-/-) mice, hepatic fibrosis was significantly attenuated in the liver of vitamin C-sufficient Gulo(-/-) mice like in wild type mice and this hepatoprotective effect of vitamin C was thought to be associated with both decreased hepatic apoptosis and necrosis. These results suggested that vitamin C had hepatoprotective effect against cholestatic liver injury. (C) 2015 Elsevier B.V. All rights reserved.</P>

Effects of O3 and H2O as oxygen sources on the atomic layer deposition of HfO2 gate dielectrics at different deposition temperatures

Lee, S.,Kim, H.,Lee, J.,Yu, I. H.,Lee, J. H.,Hwang, C. Royal Society of Chemistry 2014 Journal of Materials Chemistry C Vol.2 No.14

Variations in the growth behavior, physical and electrical properties, and microstructure of the atomic layer deposited (ALD) HfO2 gate dielectrics were examined with two types of oxygen sources: O-3 and H2O for the given Hf-precursor of Hf[N(CH3)(C2H5)](4). The ALD temperature windows for O-3 and H2O were 240-320 degrees C and 200-280 degrees C, respectively, with the growth rate of HfO2 using O-3 being higher than that of the films using H2O within the ALD window. While the film density of HfO2 using O-3 decreased, the film density of HfO2 using H2O increased with the decreasing ALD temperature. As the deposition temperature decreased, the amount of impurity in the HfO2 film with the O-3 oxidant increased due to the insufficient reaction, which led to the crystallization of the HfO2 film into the tetragonal structure after the post-deposition annealing at 600 degrees C. The films with a lower density and a higher carbon-impurity concentration retained the portion of the tetragonal phase (similar to 30%) to the highest annealing temperature of 1000 degrees C. However, the HfO2 films grown at 200 degrees C with H2O showed the best electrical performance, which could be ascribed to the highest density, low impurity concentration, and negligible involvement of the interfacial low dielectric layer.

Characterization of Insertional Variation of Porcine Endogenous Retroviruses in Six Different Pig Breeds

Jung, W.Y.,Yu, S.L.,Seo, D.W.,Jung, K.C.,Cho, I.C.,Lim, H.T.,Jin, D.I.,Lee, Jun-Heon Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.10

Pigs may need to be exploited as xenotransplantation donors due to the shortage of human organs, tissues and cells. Porcine endogenous retroviruses (PERVs) are a significant obstacle to xenotransplantation because they can infect human cells in vitro and have the potential for transmission of unexpected pathogens to humans. In this research, 101 pigs, including four commercial breeds (23 Berkshire, 13 Duroc, 22 Landrace and 14 Yorkshire pigs), one native breed (19 Korean native pigs) and one miniature breed (10 NIH miniature pigs) were used to investigate insertional variations for 11 PERV loci (three PERV-A, six PERV-B and two PERV-C). Over 60% of the pigs harbored one PERV-A (907F8) integration and five PERV-B (B3-3G, B3-7G, 742H1, 1155D9 and 465D1) integrations. However, two PERV-A loci (A1-6C and 1347C1) and one PERV-B locus (B3-7F) were absent in Duroc pigs. Moreover, two PERV-C loci (C2-6C and C4-2G) only existed in Korean native pigs and NIH miniature pigs. The results suggest that PERV insertional variations differ among pig breeds as well as among individuals within a breed. Also, the results presented here can be used for the selection of animals that do not have specific PERV integration for xenotransplantation research.

Valorization of lignocellulosic fibres of paper waste into levulinic acid using solid and aqueous Brønsted acid

Chen, Season S.,Wang, Lei,Yu, Iris K.M.,Tsang, Daniel C.W.,Hunt, Andrew J.,Jé,rô,me, Franç,ois,Zhang, Shicheng,Ok, Yong Sik,Poon, Chi Sun Elsevier 2018 Bioresource technology Vol.247 No.-

<P><B>Abstract</B></P> <P>This study aims to produce levulinic acid (LA) from paper towel waste in environment-friendly and economically feasible conditions, and evaluate the difference using solid and aqueous Brønsted acids. Direct dehydration of glucose to LA required sufficiently strong Brønsted acidity, where Amberlyst 36 demonstrated rapid production of approximately 30Cmol% of LA in 20min. However, the maximum yield of LA was limited by mass transfer. In contrast, the yield of LA gradually increased to over 40Cmol% in 1M H<SUB>2</SUB>SO<SUB>4</SUB> at 150°C in 60min. The SEM images revealed the conversion in dilute acids under microwave at 150°C resulting in swelling structures of cellulose, which were similar to the pre-treatment process with concentrated acids. Further increase in reaction temperature to 200°C significantly shortened the reaction time from 60 to 2.5min, which saved the energy cost as revealed in preliminary cost analysis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 30% of levulinic acid (LA) yielded from paper towel over Amberlyst 36 in 20min. </LI> <LI> Maximum yield of LA was comparable using dilute sulphuric acid at 150 and 200°C. </LI> <LI> Cellulose underwent swelling in dilute acid with microwave heating at 150°C. </LI> <LI> Conversion at 200°C shortened reaction time and reduced total energy consumption. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Quest for Missing Proteins: Update 2015 on Chromosome-Centric Human Proteome Project

Horvatovich, Pé,ter,Lundberg, Emma K.,Chen, Yu-Ju,Sung, Ting-Yi,He, Fuchu,Nice, Edouard C.,Goode, Robert J.,Yu, Simon,Ranganathan, Shoba,Baker, Mark S.,Domont, Gilberto B.,Velasquez, Erika,Li, D American Chemical Society 2015 Journal of Proteome Research Vol.14 No.9

<P>This paper summarizes the recent activities of the Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed “missing proteins”) in biological samples that lack sufficient experimental evidence at the protein level for confident protein identification. The C-HPP also aims to identify new protein forms that may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration forms the basis of the C-HPP’s activities; therefore, we have summarized some of the key approaches and their roles in the project. We present new analytical technologies that improve the chemical space and lower detection limits coupled to bioinformatics tools and some publicly available resources that can be used to improve data analysis or support the development of analytical assays. Most of this paper’s content has been compiled from posters, slides, and discussions presented in the series of C-HPP workshops held during 2014. All data (posters, presentations) used are available at the C-HPP Wiki (<uri xlink:href='http://c-hpp.webhosting.rug.nl/' xlink:type='simple'>http://c-hpp.webhosting.rug.nl/</uri>) and in the Supporting Information.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2015/jprobs.2015.14.issue-9/pr5013009/production/images/medium/pr-2014-013009_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr5013009'>ACS Electronic Supporting Info</A></P>

Analysis of Plasma Free Fatty Acid Cyanomethyl Derivatives by GC-NPD for the Diagnosis of Mitochondrial Fatty Acid Oxidation Disorders

Yoon, H. R.,Paik, M. J.,Shin, H. S.,Yu, C.,P.Rinaldo 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1

Early diagnosis of fatty acid oxidation (FAO) disorders is important to reduce severe morbidity and mortality. Although analysis of plasma free fatty acids (FFAs) is frequently performed using stable isotope-dilution gas chromatography-mass spectrometry (GC-MS), there are institutions where the required instrumentation is not available to support a rapid work-up of acutely ill patients. For this reason, we have developed a novel cyanomethyl derivatization method for FFAs which is followed by GC analysis of the resulting esters using nitrogen-phosphorus detector (NPD) for the rapid diagnosis of mitochondrial fatty acid oxidation disorders. FFAs were extracted from plasma and derivatized to the cyanomethyl ester by heating with bromoacetonitrile at 60℃ for 30 min GC-NPD analysis was then performed. The mean recoveries of C6:0-C18:0 FFAs were between 87% abd 96%. The method detection limits (S/N = 3) were 0.1 - 0.5ng for C6:0-C14:0 FFAs, and 0.001 - 0.01ng for C16:0-C18:0 FFAs. We succesfully performed differential diagnosis of representative FAO disorders from the confirmed patient's plasmas. This simple method offers cost-effective and time-saving alternative to GC-MS for the biochemical diagnosis of selected FAO disorders.

Influence of the Change in Oxidation Number of Mn on Magnetic Properties of <inline-formula> <tex-math notation='TeX'> <tex> ${\rm BaTi}_{{1}-{x}}{\rm Mn}_{x}{\rm O}_{3}$ </tex> </tex-math></inline-formula>

Phan, T. L.,Thanh, T. D.,Ho, T. A.,Thang, P. D.,Yu, S. C. IEEE 2014 IEEE transactions on magnetics Vol.50 No.4

<P>We prepared BaTi<SUB>1-x</SUB>Mn<SUB>x</SUB>O<SUB>3</SUB> samples (x = 0 and 0.05) by standard solid-state reaction at 700°C and 900°C in an Ar ambient to change oxidation number of Mn dopants. The fabricated samples were then studied structural characterization and electronic structures by means of X-ray diffraction and absorption, and Raman scattering and electron-spin-resonance spectrometers. We found oxidation numbers 2+ and 3+ of Mn ions coexisting in BaTi<SUB>1-x</SUB>Mn<SUB>x</SUB>O<SUB>3</SUB> with x = 0.05, where the Mn<SUP>2+</SUP>/Mn<SUP>3+</SUP> ratio is about one for the sample annealed at 700°C. These Mn ions prefer locating at the Ti site in the tetragonal BaTiO<SUB>3</SUB> host lattice. In particular, there is a change in the oxidation number of Mn<SUP>2+</SUP> → Mn<SUP>3+</SUP> when the annealing temperature changes from 700°C to 900°C. Their magnetic properties are accordingly changed. Meanwhile, annealing pure BaTiO<SUB>3</SUB> in an Ar ambient at 700°C and 900°C does not lead to ferromagnetic (FM) order; they are almost diamagnetic. With the obtained results, we believe that FM order in Mn-doped BaTiO<SUB>3</SUB> annealed in an Ar ambient is associated with exchange interactions of Mn<SUP>2+</SUP> ions mediated by oxygen vacancies rather than associated with Mn<SUP>3+</SUP> ions.</P>

승화법에 의한 CdS 단결정 성장

정태수,김현숙,유평렬,신영진,신현길,김택성,정철훈,이훈,신영신,강신국,정경수,홍광준,Jeong, T. S.,Kim, H. S.,Yu, P. Y.,Shin, Y. J.,Shin, H. K.,Kim, T. S.,Jeong, C. H.,Lee, H.,SHin, Y. S.,Kang, S. K.,Jeong, K. S.,Hong, K. J. 한국결정성장학회 1993 韓國結晶成長學會誌 Vol.3 No.2

수직 2단 전기로를 제작하고 결정성장관에 꼬리관을 연결하여 seed 결정없이 승화 방법으로 CdS 결정을 성장하였다. 이때 시료부분과 성장부분의 온도차 ${\Delta}T$가 이론적인 값 $14.7^{\circ}C$와 비교해서 실험적으로 얻은 값이 $15^{\circ}C$ 로 아주 일치하는 값을 나타내었다. 이때 꼬리관의 온도를 $110^{\circ}C$로 시간당 0.38mm 정도로 빨리 결정성장관을 끌어 올려 결정을 성장하였다. 분말법의 X-선 회절무늬와 Laue 배면 반사법의 Laue 무늬로부터 성장된 결정이 육방정이고 결정성장관의 길이 방향으로 c축을 갖는 단결정임을 확인하였다. 또한 CdS 단결정은 상온에서 전자 이동도와 운반자 밀도는 각각 $316cm^2/V{\cdot}sec$와 $2.90{\times}10^{16}cm^{-3}$정도이였다. We has made 2-zone vertical electric furnace and has been grown CdS single crystal by sublimation method in crystal growth tube with tail tube without seed crystal for growth. While it has been growing, temperature difference ${\Delta}T$ of source and growth part has nearly agreed with theoritical value $14.7^{\circ}C$and experimental value $15^{\circ}C$ Then, crystal of best quality has been grown, when temperature of tail tube has been $110^{\circ}C$, in spite of quickly pulling up crystal growth tube a degree O.38mm per hour. The grown crystal have had hexagonal structure and single crystal with c-axis to length of crystal growth tube from X- ray diffraction pattern of powder method and Laue pattern of back reflection Laue method. Also, the mobility and carrier density from Hall effect measurement have been $316cm^2/V{\cdot}sec$ and $2.90{\times}10^{16}cm^{-3}$ at the room temperature, respectively.

Three Essays by C. D. Pi

C. D. Pi,Beong-Cheon Yu(번역자),Mun Sang-d?k(번역자),Kim Chin-man(번역자) The Academy of Korean Studies 1968 Korea Journal Vol.8 No.3