갈근이 뇌허혈 손상 흰쥐의 해마신경세포 손상에 미치는 영향

부일권,김연섭 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objective : This study investigated a neuroprotective effect of herariae Radix on cerebral ischemia. Method : The global cerebral ischemia was induced by bilateral common carotid arteries occlusion under hypotension (40 mmHg) in Sprague-Dawley rats. After the treatment of Puerariae Radix extract, changes of the pyramidal neurons in CAl region of the hippocampus were observed histologically. Results :l. The significant decrease of numbers of pyramidal neurons in CA1 region of the hippocampus resulted by cerebral ischemia. Then herariae Radix treatment demonstrated significant increase of numbers of pyramidal neurons in CA1 region as compared with control group. 2. The significant decrease of pyramidal layer thickness in CA1 region of the hippocampus resulted by cerebral ischemia. Then herariae Radix treatment demonstrated significant increase of pyramidal layer thickness in CAI region as compared with control group. Conclusion : These results suggested that Puerariae Radix reveals the neuroprotective effect through the control of noxious stress stimulations to neurons.

하수오와 백수오가 난소적출로 유발된 흰쥐의 골다공증 예방효과에 미치는 영향

김민정,서부일,신순식,박지하 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : The present study has been undertaken to investigate the effects of Polygoni Multiflori Radix and Cynanchi Wilfordii Radix on prevention of osteoporosis in ovariectomized rats. Methods : In this experiment, the rats were ovariectomized. Rats were administered by 2 kinds of medicine, Polygoni Multiflori Radix and Cynanchi Wilfordii Radix. The levels of bone mineral density, osteocalcin, ALP, phosphorus, calcium in serum, calcium, phosphorus in urine, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results : The levels of spinal bone mineral density was significantly increased in comparison with OVX group at 6 weeks in Polygoni Multiflori Radix group. The levels of serum ALP showed significant decrease in comparison with OVX group at 3 weeks in Polygoni Multiflori Radix group, and showed significant decrease in comparison with OVX group at 3, 6 weeks in Cynanchi Wilfordii Radix group. The levels of serum phosphorus showed significant decrease in Comparison with OVX group at 3, 6 weeks in Polygoni Multiflori Radix and Cynanchi Wilfordii Radix group. The levels of femoral and fibula-tibial calcium showed significant increase in comparison with OVX group in Polygoni Multiflori Radix and Cynanchi Wilfordii Radix group. The levels of femoral phosphorus showed significant increase in comparison with OVX group at 3 weeks in Polygoni Multiflori Radix and Cynanchi Wilfordii Radix group. Conclusions : Reviewing these experimetal results, it appears that Polygoni Multiflori Radix and Cynanchi Wilfordii Radix have efficacy on prevention of osteoporosis. And further study should be conducted to illustrate in depth the curing osteoporosis.

盛土下部 軟弱地盤의 沈下 및 變位 擧動分析

김무일,성부경 서울産業大學校 2000 논문집 Vol.51 No.2

As embankment constructions on the soft ground proceed, many geotechnical problems concerning the ground stability and settlements begin to occur. Generally, soil has nonlinear stress-strain relation and its behavior depends on initial stress condition and stress history. There are many uncertain factors in soil. So, it is very difficult to predict the constitutive relation precisely. Results of the field data obtained by geotechnical investigations and field instrumentations were performed to investigate the deformational characteristics of the soft grounds. Theoritical calculations and numerical anaylsis were also performed. Many constitutive models on soil are proposed by several scientists. This study uses Modified Cam-Clay model among these models to analysis of the settlement and displacement of the soft ground under the embankment. Numerical analysis is conducted using CRISP which made by Cambridge University. Analysis of the settlement and displacement of the soft ground under the embankment with the finite element tenchnique using CRISP program made good predictions of the settlement and lateral displacement.

韓茵蔯蒿湯이 알코올 투여로 유발된 흰쥐의 고지혈증과 간 손상의 예방에 미치는 영향

金株坤,徐富一,崔善美,崔弘植 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Objectives : This study was performed to compare the effect of water extracts from Hanlnjinho-tang on hyperlipidemia and liver damage induced by alcohol. Methods : Except for the normal group, we fed rat on 25% alcohol for 55 days. And each extract was administrated for the same period. We measured the serum component in rat's blood, body weight and weight of liver. Results : 1. At first, we observed effects of Hanirijinho-tang on hyperlipidemia induced by alcohol. Hanirjinho-tang group showed significant decrease of total cholesterol and triglyceride in comparison with those of the control group. Haninjinho-tang group showed significant increase of HDL-cholesterol in comparison with those of the control group. Haninjinho-tang group didn't show significant increase of body weight in comparison with those of the control group in 4weeks and 8weeks. 2. At second, we observed effects of Haninjinho-tang on liver damage induced by alcohol. Haninjinho-tang group showed significant decrease of LDH, GOT in comparison with those of the control group. Haninjinho-tang group didn't show significant change of ALP, GPT in comparison with those of the control group. Haninjinho-tang group showed significant increase of liver weight in comparison with those of the control group. Conclusions : Reviewing these experimental results, it appears that water extracts from Haninjinho-tang have pharmaceutical efficacy on hyperlipidemia and liver damage induced by alcohol. Therefore further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.

비만 치료 및 예방에 대한 한약의 효능 연구(Ⅱ) : 4종 처방이 비만 흰쥐의 면역조직화학적 변화에 미치는 영향

金蓮燮,卞晟僖,徐富一,金相贊,國萬,趙應行 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1

We had experiment with several prescriptions in order to find cure and prevention effects for an obesity. We fed control gmup rats high fat diet and administered normal saline for Bweeb. And we fed experimental gmup ram high fat diet and administered exmct of four prescriptions for Bweek. The result were as follows: 1. The size of the epididymal fat cell of rats in Bikam-whan, Chekamhanghyuluiyiin-rang and Chekambohyulansin-tang groups showed a significant decrease in comparison with control group. 2. The fat drops area in hepatic lobule of rats in Bikam-whan, Cheknmhanghyuluiyiin-rang, Chekambohyulonrin-tang, and Chekambangpungrongseong-san groups showed a significant decrease in comparison with control group. 3. The area of the insulin granuls in islet of rats in Bikam-who4 Chekamhnnghyuluiyiin-tang and Chekambohyulonrin-tong group showed a significant decrease in comparison with control group. According to above these results, Bikam-whan. Cheknmhonghyuluiviin-tnng and Chekambohyulonrin-tang have shown to be capable of cure and prevent for an obesty.

韓國産 紅花子와 中國産 紅花子가 鎭痛 , 消炎 및 關節炎에 미치는 效果

서부일,이은숙,박지하,김상찬,변부형,최호영 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

This experimental study was done to compare the efficacy of Korean and Chinese Carthami Semen on analgesic effect and remedy of inflammation and arthritis. The results were as follows; 1. In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in analgesic action on the writhing syndrome in mice induced by Acetylcholine HC1, Korean and chinese Carthami Semen extract group showed significant decrease in comparison with control group. 2. In Anti-inflammatory effects, Chinese Carthami Semen extract group showed significant decrease at 150 and 180 minutes after inducing paw-edema in comparison with control group. 3. In the method of adjuvant arthritis, orally administered for 21 days, Korean Carthami Semen extract group showed significant inhibition of the hind paw edema after 1 day and 21 days. Whereas Chinese Carthami Semen extract group showed some reduction, but the results didn's show significant decrease. 4. In the level of leucocytes count, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in the level of total protein. CRP and glucose, Korean and Chinese Carthami Semen extract group didn't showed significant change in comparison with control group.

夏枯草의 免疫調節作用 및 알레르기 低減化에 關한 硏究

徐富一,金先熙 대한본초학회 1997 大韓本草學會誌 Vol.12 No.1

The results of Prunellae Spica on immune response and anti-allergic reaction were as follows. 1. In Prunellae Spica group, hemagglutinin titer and hemolysin titer were increased in comparison with control group. But the results were not recognized as having significance. 2. The number of Plaque forming cells were increased in case of Prunellae Spica. But the results were not recognized as having significance. 3. The number of Rosette forming cells were increased in case of Prunellae Spica group, and the results were significant. 4. In experimentation of phagocytic activity in peritoneal exudate cells and monolymphocytus cells, Prunellae Spica group showed increase. But Prunellae Spica group in spleen cells showed decrease on the contrary. But these results were not recognized as having significance. 5. In Prunellae Spica group, results of PCA response were increased in case of 2 week, 4 week, 6 week on the contrary. 6. In experimentation on histamine contents, Prunellae Spica group showed derease at all agent contact. But these results were of no importance. From above these results, Prunellae Spica group showed good effects on immunoreaction. And Prunellae Spica group showed effects on anti-allergic reaction Ⅰ.

慶南 梁山地方의 工業化에 따른 氣溫上昇에 대한 考察

李富龍,田炳日,金有根 부산대학교 환경문제연구소 1991 環境硏究報 Vol.9 No.-

The temperature variations due to industrialization in Yangsan area are investigated using the 10 years(1980∼1989) temperature data and compared with those of Jinju. The major results are as follows: (1) The rates of increase of mean and minimum temperatures were more pronounced in Yangsan area than in Jinju area, and the increasing rates were always positive for every month. (2) There exists close correlation between the growth of population and increase of minimum temperature, and between the growth of population and the increase of mean temperature. Furtheromore, close correlations between the increase of those temperature and other industrial factors are also indentified.

구자가 卵巢摘出로 誘發된 흰쥐의 骨多孔症에 미치는 影響 (2)

徐富一,金先熙,卞晟僖,金美麗 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

This study was performed to investigate the effects of Allii Tuberosi Semen(菲子) on osteoporosis after ovariectomy in rats. Control group received a daily po administration of normal saline for 4 weeks after oophorectomy(OVX). The other experimental group received a daily po administration of water extract of Allii Tuberosi Semen for 4 weeks after oophorectomy(OVX-ATS). Therefore, we measured levels of calcium, phosphorus, ash weights in femur. These results were as follows : 1. In the level of bone phosphorus, the result of OVX was 63.85±1.86㎎/㎗, but the result of OVX-ATS was 68.18±0.85㎎/㎗. But there was no statistical significance. 2. In the level of bone calcium, the result of OVX was 141.66±2.19㎎/㎗, but the result of OVX-ATS group was 164.82±3.74㎎/㎗. And the result of OVX-ATS showed significant increase in comparison with OVX. 3. In the level of bone ash weight, the result of OVX was 0.31±0.005(g), but the result of OVX-ATS was 0.33±0.002(g). And the result of OVX-ATS showed significant increase in comparison with OVX. The observations from these studies and the past studies(=A study on the effects of Allii Tuberosi Semen in ovariectomized osteoporosis of rats) suggest possible protective effect of Alli Tuberosi Semen water extract against bone loss in ovariectomized rats.

牧楡湯이 人體 肺癌細胞의 變化에 미치는 影響에 關한 分子生物學的 硏究

李炯周,金相贊,徐富一,金先熙,卞晟僖 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

This experimental study was performed to investigate the effects of Mokyou-Tang on cell cycle and gene expressions of human lung cancer cell. I examined the cell cycle progression and cell cycle-related gene expression in A549 using a flow cytometry and a quantitative PT-PCR analysis. The treatment groups, in accordance with concentration of Mokyou-Tang were classified as 3 group(MYT 100㎕/㎖, MYT 500㎕/㎖, MYT 1,000㎕/㎖), and according to treatment time, were classified as 2 group(24hours, 48hours). The results were summarized as follows; 1. In cell cycle stage, MYT 100㎕/㎖(48hours) and 1000㎕/㎖(48hours) groups were increased as compared with control group, especially 1000㎕/㎖(48hours) group showed eminent increase. 2. In p53 gene, MYT 500㎕/㎖(48hours) and 1000㎕/㎖(48hours) groups showed eminent suppressive effect to cancer cell. 4. CDKNI gene was decreased in all treatment groups, this mean that MYT has suppressive effect to proliferation of cancer cell. 4. In GADD45 gene was decreased in all treatment groups, this mean that MYT has suppressive effect to proliferation of cancer cell. The results suggest that MYT promotes G1 cell cycle arrest and suppresses the proliferation of cancer cell. Thus, it seems likely that MYT has suppressive effect to proliferation of lung cancer cell.