공단지역 및 청정지역 식물 잎권의 잎표면세균 및 내산성세균의 분포

안종훈,방숙진,한남정,송왕영,황소영,이인수,박성주 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

산성강하물의 영향을 받는 대천공단지역과 영향을 받지 않는 청정지역인 대전 계족산 자연휴양림에서 자라는 밤나무(Castanea crenat)의 잎표면에서 서식하는 총세균수, 생존세균수, 종속영양세균수, 내산성세균수를 1996년 8월부터 1997년 8월까지 5회에 걸쳐 조사하였다. 공단지역 잎표면 평균 총세균수, 생존세균수 및 종속영양세균수는 각각 9.9×10^(5) cell/㎠, 1.6×10⁴cell/㎠, 7.1×10³cell/㎠,로서 청정지역에 비하여 각각 1.5배, 2배, 2.6배 정도로 관찰되었다. MPN법으로 측정한 pH 5.6애서의 잎표면 내산성세를수는 공단지역 3.3×10⁴, 청정지역 3.4×10⁴MON/㎠로 거의 같았고, pH 4.0에서의 내산성세균수는 공단지역애서 1.9×10^-(-1)MPN/㎠인 반면 청정지역에서는 전혀 검출되지 않았다. pH 3.0에서의 내산성 잎표면세균수는 공단지역과 청정지역의 잎권 어느 곳에서도 검출되지 않았다. 한편 계절별 잎표면세균수의 분포는 대체로 잎이 나기 시작하여 크기가 가장 작은 5월에 최대를, 그리고 낙엽이 지는 11월에 최소를 나타내었다. 이런 결과는 공단지역의 대기오염물질의 침적이 주변의 식물 잎표면 세균수를 감소시키지는 않으며, 특히 산성강하물의 영향으로 내산성세균수가 증가함을 보여주고 있다. Total, direct viable count, and acid-tolerant epiphytic bacterial population sizes were quantified on leaves of chestnut tree (Castanea crenata S. et Z.) near Taejon Industrial Estate affected by acid precipitation and deposition as well as in the clean natural forest area, Mt. Kyejok, in Taejon city from August 1996 to August 1997. Geometric mean numbers of total, direct viable count, and acid-tolerant epiphytic bacteria were 9.9×10^(5) cell/㎠, 1.6×10^(6)cell/㎠, and 7.1×10³cfu/㎠ respecfvely, being 1.5, 2, and 2.6 times those in the clean area. Acid-to-lerant epiphytic bfcterial numbers at pH 5.6 by MPN method were 3.3×10" in the industrial area, about the same as the number,3.4×104 MPNicni, of the clean area. Acid-tolerant bacterial number at pH 4.0 was 1.9×10^(-1)MPN/㎠ in the industrial area, whereas none was detected in the clean area. Acid-tolerant bacteria at pH 3.0 were not detected at all in the industrial area as well as in the clean area. Epiphytic bacterial population sizes were generally the greatest in May when leaves are emerged and grew but the lowest in November when defoliation occurs. These results showed that air pollutant deposition on leaves did not cause a deuease of epiphytic bacteria at least and acid deposition on leaves did cause an increase of acid-tolerant bacteria.

홍국 쌀 첨가량을 달리하여 제조한 의류의 이화학적 및 관능적 특성 변화

김철암(Tie-Yan Jin),김은수(Eun-Soo Kim),은종방(Jong-Bang Eun),왕승진(Seung-Jin Wang),왕명현(Myeong-Hyeon Wang) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.3

멥쌀에 홍국 쌀을 각각 0, 10, 20, 30, 40% 첨가하여 제조한 약주의 이화학적 특성 및 관능적 특성의 변화를 조사하였다. 홍국 쌀 첨가량의 증가에 따라 pH는 감소되었고 총산의 함량은 증가된 것으로 나타났다. 총당의 함량은 홍국 쌀 첨가량의 증가에 따라 증가하였고 알코올 함량은 감소하였다. 색도 중 L값은 홍국 쌀 첨가량의 증가에 따라 감소되었으나 a와 b값은 증가한 것으로 나타났다. 향기성분은 esters, aldehydes, alcohols, acids로 분류되었고 과일향의 주성분인 esters의 함량은 10% 흥국 쌀을 첨가한 시험구에서 제일 많이 생산된 것으로 나타났다. 관능겸사 결과 전체적 기호도에서 10% 홍국 쌀을 첨가하여 제조한 약주가 제일 우수한 것으로 나타났다. 이화학적 특성이나 관능적 특성으로 보아 10% 홍국 쌀을 첨가하는 약주를 제조하는 것이 적합하다고 생각된다. The physicochemical and sensory characteristics of rice wine, Yakju, made with different additions of red yeast rice (0, 10, 20, 30, and 40%) were investigated. The pH of the rice wine decreased and the total acidity increased with greater additions of red yeast rice. Furthermore, the total sugar content increased and the alcohol content decreased with increasing amounts of red yeast rice. The color L-value diminished and the color a- and b-values increased with increasing amounts of red yeast rice. Among the volatile flavor compounds, esters, aldehydes, alcohols, and acids were the most important aroma-active compounds identified in the rice wine. The sample with the 10% red yeast rice addition had the highest ester content, which is a primary component in fruit fragrance. The highest overall acceptability values in the sensory test for color, flavor, taste, and overall favorite were 5.10, 5.75, 5.15, 4.65, and 4.50, respectively, with the addition of 10% red yeast rice.

Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil

Wang, Chong-Zhi,Hou, Lifei,Wan, Jin-Yi,Yao, Haiqiang,Yuan, Jinbin,Zeng, Jinxiang,Park, Chan Woong,Kim, Su Hwan,Seo, Dae Bang,Shin, Kwang-Soon,Zhang, Chun-Feng,Chen, Lina,Zhang, Qi-Hui,Liu, Zhi,Sava-Se The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.2

Background: Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regulation mechanisms. Methods: Ginseng berry polysaccharide extract (GBPE) and purified ginseng berry polysaccharide portion (GBPP) were used to evaluate their activities on human HCT-116 and HT-29 CRC cell proliferation. Interleukin-8 secretion analysis was performed on HT-29 cells. Naive CD4 cell isolation and T-helper cell differentiation were performed and determined using flow cytometry for Th1 and Treg in addition to cell cycle and apoptotic investigation. Results: GBPE and GBPP significantly inhibited interleukin-8 secretion and cancer cell proliferation, inhibited CD4⁺IFN-γ⁺ cell (Th1) differentiation, and decreased CD4⁺FoxP3⁺ cell (Treg) differentiation. Compared to the GBPE, GBPP showed more potent antiinflammatory activities on the malignant cells. This is consistent with the observation that GBPP can also inhibit Th1-cell differentiation better, suggesting that it has an important role in antiinflammation, whereas Treg cells hinder the body's immune response against malignancies. Supported by cell cycle and apoptosis data, GBPE and GBPP, at various degrees, remarkably enhanced the anticancer activities of 5-fluorouracil. Conclusion: Data from this project suggested that Asian ginseng berry potentially has clinical utility in managing enteric inflammation and suppressing CRC through immunomodulation mechanisms.

Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens

Wang, Hye-young,Jin, Hyunwoo,Bang, Hyeeun,Choi, Yeon-Im,Park, Eun-mi,Koh, Won-jung,Lee, Hyeyoung The Korean Society of Clinical Microbiology (KAMJE 2011 Annals of clinical microbiology Vol.14 No.3

Establishment of Tetraploid Embryo System by Electrofusion of 2-Cell Embryo Derived from Bovine IVP

Erdan Wang,Han-Seul Park,Jea-Il Bang,Su-Jin Jo,A-Na Ha,Kyeong-Lim Lee,Il-Keun Kong,Wen-Fa Lv 한국동물생명공학회(구 한국동물번식학회) 2012 발생공학 국제심포지엄 및 학술대회 Vol.2012 No.1

중국인터넷쇼핑몰 환경에서 이용자의 혁신성이 지각된 사용성 및 고객충성도에 미치는 영향에 관한 연구: 지각위험 조절효과 중심으로

진방(Jin Bang),왕레이(Wang Lei),전상택(Sang-Taek Jeun) 조선대학교 지식경영연구원 2020 기업과 혁신연구 Vol.43 No.1

소비자들의 욕구와 필요가 다양해지고, 디지털 기술과 정보통신 기술의 급속한 진화와 함께 경제의 글로벌화가 가속화 되면서 제품의 수명과 교체 주기가 짧아지고, 소비자는 혁신적 제품을 요구하게 되었다. 제품경쟁이 치열해지는 기업 환경은 기업의 발전과 생존에 지속적인 기회와 위협의 요인으로 작용하고 있으며, 기업들은 시장에서 새로운 기술을 적용하여 독특함과 신기성을 추가한 신제품을 출시하여 소비자들의 니즈를 충족시키는 제품을 만들어 구매를 촉진하고자 노력한다. 소비자들은 새로운 제품에 대한 욕구가 증가하고 있으며 이러한 환경에 대처하는 기업은 제품성공을 위해 혁신성 신제품을 생산하여 소비자에게 새로운 가치를 창조하고자 한다. 따라서 고객의 혁신성향과 소비자의 가치 욕구와 니즈를 충족하는 혁신적 제품은 제품 차별화와 세분화시장의 적응성을 높여 기업의 경쟁력을 강화시킬 수 있기 때문이다. 본 연구는 중국인터넷쇼핑몰 환경에서 이용자의 혁신성이 지각된 사용성과 고객충성도에 미치는 영향관계를 살펴보며, 이용자의 지각된 위험이 이용자의 혁신성과 지각된 사용성 간에 조절효과 역할이 미치는지 알아보기 위하여 연구 과제를 도출하였다. As consumers" needs and needs become diverse, and the globalization of the economy accelerates with the rapid evolution of digital technology and information and communication technology, the life and replacement cycle of products are shortened and consumers are demanding innovative products. The corporate environment in which product competition is fierce is a factor of continuous opportunity and threat to the development and survival of the company. Companies try to promote the purchase by making products that meet the needs of consumers by launching new products that add uniqueness and novelty by applying new technology in the market. Consumers are increasingly desire for new products, and companies that cope with these environments are trying to create new value for consumers by producing innovative new products for product success. Therefore, innovative products that meet customer innovation tendency, consumer value desire and needs can enhance product competitiveness and adaptability to segmentation market. This study examined the effect of user"s innovativeness on perceived usability and customer loyalty in the Chinese Internet shopping mall environment, and drew research tasks to find out whether the perceived risk of users has a moderating effect between user"s innovativeness and perceived usability.

Evaluation of a PCR-Reverse Blot Hybridization Assay to Identify Six Dermatophytes Predominant in the Republic of Korea

Hyunwoo Jin,Hyunjung Kim,Sunghyun Kim,Yeonim Choi,Hyeeun Bang,Sangjung Park,Hyeyoung Wang,Jang-Ho Lee,In Ho Jang,Young-Kwon Kim,Hyeyoung Lee 대한의생명과학회 2014 Biomedical Science Letters Vol.20 No.3

Accurate and rapid diagnosis of dermatophytosis, a disease whose prevalence has been steadily increased, is important for successful treatment. Current laboratory methods for diagnosing dermatophytosis rely on KOH mount and fungal culture method. However, these methods have low sensitivity and are time-consuming (2~4 weeks to diagnosis). In our previous study, a rapid molecular diagnostic assay (PCR-reverse blot hybridization assay, REBA) was developed to identify the following 6 main species of dermatophytes: Trichophyton rubrum, T. mentagrophytes, T. tonsurans, Microsporum canis, M. gypseum, and Epidermophyton floccosum. However, the REBA required more evaluation to validate its use in clinical examinations. The aim of the present study was to evaluate and validate the ability of the PCR-REBA to successfully identify dermatophytes in clinical isolates from dermatophytosis patients. Both conventional identification methods and the PCR-REBA were used to assess the presence of species of dermatophytes in 148 clinical isolates. The results of the two approaches were compared, and discrepancies between the two approaches were resolved by fungal ITS1 sequence analysis. T. rubrum was the most prevalent dermatophyte identified by conventional identification methods (118/148, 79.7%) and the PCR-REBA (131/148, 88.4%). The overall rate of consistency between conventional identification methods and the PCR-REBA was 79.0% (117/148 samples). Fungal ITS1 sequence analysis showed that PCR-REBA results were correct for 93.5% (29/31) of the discrepant samples. The PCR-REBA is rapid, sensitive, and highly specific compared with conventional identification methods. Thus, the PCR-REBA is a potentially useful tool for identifying dermatophytes in clinical settings.

Expression of cellular retinoic acid-binding protein-I (CRABP-I) in the cerebrospinal fluid of adult onset moyamoya disease and its association with clinical presentation and postoperative haemodynamic change

Jeon, Jin Sue,Ahn, Jun Hyong,Moon, Youn-joo,Cho, Won-Sang,Son, Young-Je,Kim, Seung-Ki,Wang, Kyu-Chang,Bang, Jae Seung,Kang, Hyun-Seung,Kim, Jeong Eun,Oh, Chang Wan BMJ Publishing Group Ltd 2014 Journal of neurology, neurosurgery and psychiatry Vol.85 No.7

<P><B>Objective</B></P><P>The elevation of cellular retinoic acid-binding protein-I (CRABP-I) has been suggested as a candidate in the pathogenesis of paediatric moyamoya disease (MMD). However, few studies have addressed CRABP-I in adult onset MMD. The aim of this study was to examine the expression of CRABP-I in the cerebrospinal fluid (CSF) of adult onset MMD, and to evaluate its association with clinical presentation and postoperative haemodynamic change.</P><P><B>Methods</B></P><P>This study examined the CSF from 103 patients: bilateral MMD, n=58 (56.3%); unilateral MMD, n=19 (18.4%); atherosclerotic cerebrovascular disease (ACVD), n=21 (20.4%); and control group, n=5 (4.9%). The intensity of CRABP-I was confirmed by western blotting and expressed as the median (25th–75th percentile). The differences in CRABP-I expression according to disease entity (unilateral MMD vs bilateral MMD vs ACVD), initial presenting symptoms (haemorrhage vs ischaemia) and postoperative haemodynamic change (vascular reserve in single photon emission CT and basal collateral vessels in digital subtraction angiography) were analysed.</P><P><B>Results</B></P><P>CRABP-I intensities in bilateral MMD (1.45(0.86–2.52)) were significantly higher than in unilateral MMD (0.91(0.78–1.20)) (p=0.044) or ACVD (0.85(0.66–1.11)) (p=0.004). No significant differences were noted based on the initial presenting symptoms (p=0.687). CRABP-I was not associated with improvement in vascular reserve (p=0.327), but with decrease in basal collateral vessels (p=0.023) postoperatively.</P><P><B>Conclusions</B></P><P>Higher CRABP-I in the CSF can be associated with typical bilateral MMD pathogenesis in adults. Additionally, postoperative basal collateral change may be related to the degree of CRABP-I expression.</P>

Identification of Differentially Expressed Proteins in Human Kidney with IgA Nephropathy

( Yeong Jin Choi ),( Eun Hui Wang ),( Kweon Heang Lee ),( Yong Bok Koh ),( Dong Chan Jin ),( Chul Woo Yang ),( Yong Soo Kim ),( Byung Kee Bang ),( Chang Suk Kang ),( Jung Ho Cha ) 대한신장학회 2003 춘계학술대회 초록집 Vol.22 No.1

위선종과 조기위암에서 투명캡을 이용한 흡인점막절제술의 임상적 유용성 평가

김진일,이강문,정인식,방춘상,박두호,이동수,김성수,김상우,송호진,성용직,한석원,양영상,최규용,왕준호 대한소화기내시경학회 2000 Clinical Endoscopy Vol.21 No.3

Background/Aims: Endoscopic aspiration mucosectomy (EAM) can always lift up the lesion by suction regardless of its location and does not injure the mucosal surface. The clinical usefulness of this method was evaluated as follows. Methods: Thirty two patients with gastric adenoma (35 lesions) and 6 patients with early gastric carcinoma were treated by EAM from March 1997 to February 1999. Their characteristics (diameter, macroscopic appearance, and histologic diagnosis) were verified at endoscopy with a biopsy specimen. The complete resection was defined as the presence of normal mucosa at the resected margin. Results: The average size of the resected specimens was 17.8 mm in antrum and 16.5 mm in body. The ratio of complete resection by location was 67% in anterior wall, 88% in posterior wall, 86% in lesser curvature and 92% in greater curvature. The complete resection rate for smaller lesions (≤15 mm) was highly distinguished from that cf larger lesions (>15 mm). No serious complication was encountered. Coclusions: EAM is suitable for the treatment of gastric tumors. Lesions 15 mm or less in diameter can be resected easily by single procedure regardless of its location.