Early IL-17A Prevention Rather Than Late IL-17A Neutralization Attenuates Toluene Diisocyanate-Induced Mixed Granulocytic Asthma

Chen Shuyu,Yu Li,Deng Yao,Liu Yuanyuan,Wang Lingwei,Li Difei,Yang Kai,Liu Shengming,Tao Ailin,Chen Rongchang 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.5

Purpose: Interleukin (IL)-17A plays a critical role in the pathogenesis of allergic airway inflammation. Yet, the exact roles of IL-17A in asthma are still controversial. Thus, the aim of this study was to dissect the roles of IL-17A in toluene diisocyanate (TDI)-induced mixed granulocytic asthma and to assess the effects of neutralizing antibody in different effector phases on TDI-induced asthma. Methods: IL-17A functions in allergic airway inflammation were evaluated using mice deficient in IL-17A (Il17a−/−) or IL-17A monoclonal antibody (IL-17A mab, intraperitoneally, 50 μg per mouse, 100 μg per mouse). Moreover, the effects of exogenous recombinant IL (rIL)-17A in vivo (murine rIL-17A, intranasally, 1 μg per mouse) and in vitro (human rIL-17A, 100 ng/mL) were investigated. Results: TDI-induced mixed granulocytic airway inflammation was IL-17A-dependent because airway hyperreactivity, neutrophil and eosinophil infiltration, airway smooth muscle thickness, epithelium injury, dysfunctional T helper (Th) 2 and Th17 responses, granulocytic chemokine production and mucus overproduction were more markedly reduced in the Il17a−/− mice or by IL-17A neutralization during the sensitization phase of wild-type (WT) mice. By contrast, IL-17A neutralization during the antigen-challenge phase aggravated TDI-induced eosinophils recruitment, with markedly elevated Th2 response. In line with this, instillation of rIL-17 during antigen sensitization exacerbated airway inflammation by promoting neutrophils aggregation, while rIL-17A during the antigen-challenge phase protected the mice from TDI-induced airway eosinophilia. Moreover, rIL-17A exerted distinct effects on eosinophil- or neutrophil-related signatures in vitro. Conclusions: Our data demonstrated that IL-17A was required for the initiation of TDI-induced asthma, but functioned as a negative regulator of established allergic inflammation, suggesting that early abrogation of IL-17A signaling, but not late IL-17A neutralization, may prevent the progression of TDI-induced asthma and could be used as a therapeutic strategy for severe asthmatics in clinical settings.

China Consensus Document on Allergy Diagnostics

Chen Hao,Li Jing,Cheng Lei,Gao Zhongshan,Lin Xiaoping,Zhu Rongfei,Yang Lin,Tao Ailin,Hong Haiyu,Tang Wei,Guo Yinshi,Huang Huaiqiu,Sun Jinlyu,Lai He,Lei Cheng,Liu Guanghui,Xiang Li,Chen Zhuanggui,Ma Ha 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.2

The prevalence of allergic diseases has increased dramatically in recent years in China, affecting the quality of life in 40% of the population. The identification of allergens is the key to the diagnosis of allergic diseases. Presently, several methods of allergy diagnostics are available in China, but they have not been standardized. Additionally, cross-sensitization and co-sensitization make allergy diagnostics even more complicated. Based on 4 aspects of allergic disease (mechanism, diagnosis procedures, allergen detection in vivo and in vitro as well as the distribution map of the most important airborne allergens in China) and by referring to the consensus of the European Society of Allergy and Clinical Immunology, the World Allergy Organization, and the important literature on allergy diagnostics in China in recent years, we drafted this consensus of allergy diagnostics with Chinese characteristics. It aims to standardize the diagnostic methods of allergens and provides a reference for health care givers. The current document was prepared by a panel of experts from the main stream of professional allergy associations in China.

Hydrothermal Hexagonal SrFe12O19 Ferrite Powders: Phase Composition, Microstructure and Acid Washing

Ailin Xia,Xuzhao Hu,Diankai Li,Lu Chen,Chuangui Jin,Conghua Zuo,Shubing Su 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.2

A series of hexagonal m-type SrFe12O19 ferrite powders were hydrothermally synthesized, and their phase composition, microstructure and magnetic properties before/after acid washing were studied. In the synthesis of these specimens, the atomic ratio of Fe/Sr (RF/S) in starting materials was set to 4, 5 and 12, respectively. When RF/S = 12, the specimen has morphology of round flat cakes, not typical hexagonal plate-like structure. The results of SEM images and XRD patterns indicate that the specimen with RF/S = 12 was mostly composed of Fe2O3. When RF/S = 4 or 5, the hexagonal plate-like SrFe12O19 ferrite powders were successfully synthesized with only a small quantity of Fe2O3 and SrCO3 impurities. It is also found that acid washing can eliminate the impurities in as-synthesized specimens effectively, and also change their topography, which enhances the saturation magnetization. However, the coercivity changed irregularly after acid washing, which is ascribed to the combination of the changed morphology, introduced stress and lattice defects.

Transition of Crack Propagation from a Transgranular to an Intergranular Path in an Overaged Al-Zn-Mg-Cu Alloy During Cyclic Loading

Xu Chen,Zhiyi Liu,Peng Xia,Ailin Ning,Sumin Zeng 대한금속·재료학회 2013 METALS AND MATERIALS International Vol.19 No.2

The fatigue crack propagation behavior in the overaged Al-Zn-Mg-Cu alloy was characterized by optical microscopy, scanning electron microscopy, transmission electron microscopy and electron backscatter diffraction. The results revealed that a fatigue crack tended to transgranularly propagate in the near-threshold regime, whereas intergranular crack propagation was dominant at the high ΔK regime. The transition of crack propagation from a transgranular to an intergranular path that occurred in the Paris regime was strongly influenced by the misorientation of adjacent grains and precipitate free zones. In addition, a crystallographic model of crack propagation was proposed to interpret the transition. The fatigue short crack propagation on a single slip plane was responsible for the formation of a transgranular propagation path in the near-threshold regime. The fatigue long crack propagation, which was conducted by a duplex slip mechanism in the Paris regime, led to the formation of fatigue striations. The formation of a zigzag crack in the near-threshold regime was ascribed to the high misorientation of adjacent grains.

A Novel Spectral Matching LED Lighting Source for Plant

Fan Cao,Ailin Chen,Zhisheng Wang,Xiaoyang He,Nianyu Zou 한국조명·전기설비학회 2017 조명·전기설비학회논문지 Vol.31 No.8

This paper deals with the spectral matching optimization by a group of narrowband high power LEDs to achieve the target spectrum required by plant growing, the correlation index is a key evaluation criteria, higher value of which is often difficult to achieve because of the poor performance of spectral matching algorithm. The bat algorithm is proposed as a spectral matching algorithm. Parameters of bat algorithm under high correlation index are selected by observing the parameter variation tendency in the spectral matching simulation. In the experimental verification, 2 kinds of control circuit are used to match the target spectrum. Experimental results shows that appropriate parameter groups of bat algorithm must select based on their relationship with the correlation index. In practical experiment, the optimal correlation index is 0.70±0.05 under conditions of 3 selected bat algorithm parameter groups. It is of significance to plant lighting in the engineering practice exploratory.

Homologous tumor cell membrane vesicles active preferential self‑recognition of tumor cells in vitro

Wu Chenghu,Yu Ailin,Chen Yue,Fan Mingbo 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.1

Cell membrane vesicles, as delivery carriers of drugs or biological agents in vivo, are an important therapeutic mode in the study of disease treatment. Tumor membrane-derived vesicles have been widely used in tumor therapy because of their good tumor enrichment effect. The most common method is the surface of nanoparticles coated with tumor cell membrane, which can effectively prolong the circulation time of particles in the blood and the enrichment of tumors. In this study, we prepared vesicles of different tumor cell membrane derivate and studied their targeting to tumors detailly. The results showed that homologous vesicles have high targeting to homologous tumor cells. The fluorescence of vesicles in homologous tumor cells was significantly higher than that in other tumor cells. This study will provide a new strategy and guidance for the clinical treatment of cancer based on the tumor cell membrane system.

Random Imperfection Method for Stability Analysis of a Suspended Dome

Xuechun Liu,Xinxin Zhan,Ailin Zhang,Xun Zh,Chen Tian 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.1

The traditional eigenvalue buckling mode method used for the stability analysis of suspended domes has been deemed unsafe and unreasonable. A random imperfection method is proposed in this paper that is suitable for the stability analysis of suspended domes. Using this method, the impact of initial imperfections, such as joint position deviation, eccentricity of members, variation in cable pretension and elastic modulus of materials, on the stability of nonlinear geometries as well as the stability of both nonlinear geometries and materials (i.e., ultimate bearing capacity) are studied. The analysis results using this method are compared to those obtained from the traditional eigenvalue buckling mode method. This study indicates that the random imperfection method can more reasonably demonstrate the impact of initial imperfections on the stability of suspended domes. Additionally, the shape and size of the initial imperfections applied by the random imperfection method are more reasonable, and the results are safer. The random imperfection method is successfully used in the stability analysis of a largespan suspended dome, and the results of this method are nearly equivalent to that of the model test, which verifies the method.

Arylazolyl(azinyl)thioacetanilide. Part 9#: Synthesis and Biological Investigation of Thiazolylthioacetamides Derivatives as a Novel Class of Potential Antiviral Agents

Peng Zhan,Xinyong Liu,Liu Wang,Hong Liu,Xuwang Chen,Xiao Li,Xin Jiang,Qiangqiang Zhang,Christophe Pannecouque,Lieve Naesens,Erik De Clercq,Ailin Liu,Guanhua Du 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

In continuation of our endeavor to develop new, potent, selective and less toxic antiviral agents, a novel series of 2-(2-amino/chloro-4-(2,4-dibromophenyl) thiazol-5-ylthio)acetamide derivatives was synthesized via an expeditious route and evaluated for their anti-HIV activities against wild-type virus and clinically relevant mutant strains, and for their anti-influenza virus activities against influenza A (H1N1 and H3N2) and influenza B in cellular assays. The selected active compounds were also assayed for their enzymic inhibitory activities. The results showed that some 2-chloro substituted thiazolylthioacetamide derivatives possessed potent activity against wild type HIV-1 and several key mutant strains (E138K, K103N, L100I) of HIV-1 in MT-4 cells with EC50 values in micromolar range. Two 2-amino substituted thiazole derivatives 8a7 and 8a8 displayed significant potency against influenza A/H1N1 in MDCK cells with EC50 values much lower than that of oseltamivir carboxylate, ribavirin, amantadine and rimantadine. Though the mechanism of actions is still unclear, these novel thiazolylthioacetamides might serve as original leads for further pharmacological investigations as potential therapeutic agents against HIV-1 or influenza virus.

Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene

Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4

The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.