Arylazolyl(azinyl)thioacetanilide. Part 9#: Synthesis and Biological Investigation of Thiazolylthioacetamides Derivatives as a Novel Class of Potential Antiviral Agents

Peng Zhan,Xinyong Liu,Liu Wang,Hong Liu,Xuwang Chen,Xiao Li,Xin Jiang,Qiangqiang Zhang,Christophe Pannecouque,Lieve Naesens,Erik De Clercq,Ailin Liu,Guanhua Du 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

In continuation of our endeavor to develop new, potent, selective and less toxic antiviral agents, a novel series of 2-(2-amino/chloro-4-(2,4-dibromophenyl) thiazol-5-ylthio)acetamide derivatives was synthesized via an expeditious route and evaluated for their anti-HIV activities against wild-type virus and clinically relevant mutant strains, and for their anti-influenza virus activities against influenza A (H1N1 and H3N2) and influenza B in cellular assays. The selected active compounds were also assayed for their enzymic inhibitory activities. The results showed that some 2-chloro substituted thiazolylthioacetamide derivatives possessed potent activity against wild type HIV-1 and several key mutant strains (E138K, K103N, L100I) of HIV-1 in MT-4 cells with EC50 values in micromolar range. Two 2-amino substituted thiazole derivatives 8a7 and 8a8 displayed significant potency against influenza A/H1N1 in MDCK cells with EC50 values much lower than that of oseltamivir carboxylate, ribavirin, amantadine and rimantadine. Though the mechanism of actions is still unclear, these novel thiazolylthioacetamides might serve as original leads for further pharmacological investigations as potential therapeutic agents against HIV-1 or influenza virus.

Bearing capacity of H-section beam wrapped with ceramsite concrete

Xuechun Liu,Kun Meng,Ailin Zhang,Tao Zhu,Cheng Yu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.5

In this study, an H-section steel beam with circular holes in a web wrapped with ceramsite concrete (SBWCC) was studied. Static load-bearing capacity tests and finite element analysis were performed on two groups of specimens with different sections. The H-section steel and wrapped ceramsite concrete were well bonded. The load-bearing capacity of the SBWCC was 10% larger than that of the pure H-section steel beam without holes in the web, except for its dead weight. The stiffness of the SBWCC was slightly larger than that of the pure H-section steel beam without holes. The wrapped ceramsite concrete avoided the elastic local instability of the steel beam flange and web. Based on the finite element model verified by experiments, the influences of hole diameter, hole spacing, and U-shaped stirrups on the flexural capacity of the specimens were analyzed. The formulas for the load-bearing capacities and short-term stiffness of the SBWCC were proposed and verified by tests and finite element analysis.

The Progress of Decellularized Scaffold in Stomatology

Zeng Ailin,Li Huiru,Liu Jianguo,Wu Mingsong 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3

The oral and maxillofacial region contains oral organs and facial soft tissues. Due to the complexity of the structures and functions of this region, the repair of related defects is complicated. Different degrees of defects require different repair methods, which involve a great combination of medicine and art, and the material requirements are extremely high. Hence, clinicians are plagued by contemporary oral repair materials due to the limitations of bone harvesting, immune rejection, low osteogenic activity and other problems. Decellularized extracellular matrix has attracted much attention as a bioactive scaffold material because of its nonimmunogenic properties, good osteogenic properties, slow release of growth factors, promotion of seed cell adhesion and maintenance of stem cell characteristics. This article reviews the sources, preparation methods, application and research progress of extracellular matrix materials in the repair of oral and maxillofacial defects to provide an overview for fundamental research and clinical development.

Random Imperfection Method for Stability Analysis of a Suspended Dome

Xuechun Liu,Xinxin Zhan,Ailin Zhang,Xun Zh,Chen Tian 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.1

The traditional eigenvalue buckling mode method used for the stability analysis of suspended domes has been deemed unsafe and unreasonable. A random imperfection method is proposed in this paper that is suitable for the stability analysis of suspended domes. Using this method, the impact of initial imperfections, such as joint position deviation, eccentricity of members, variation in cable pretension and elastic modulus of materials, on the stability of nonlinear geometries as well as the stability of both nonlinear geometries and materials (i.e., ultimate bearing capacity) are studied. The analysis results using this method are compared to those obtained from the traditional eigenvalue buckling mode method. This study indicates that the random imperfection method can more reasonably demonstrate the impact of initial imperfections on the stability of suspended domes. Additionally, the shape and size of the initial imperfections applied by the random imperfection method are more reasonable, and the results are safer. The random imperfection method is successfully used in the stability analysis of a largespan suspended dome, and the results of this method are nearly equivalent to that of the model test, which verifies the method.

Molecular mechanism of poisoning to honeybees (Apis mellifera L.) after ingestion of Camellia oleifera nectar

Wang Yang,Liu Xiaohua,Wang Yiming,Jin Mingxia,Xie Guai,Zhong Qiuping,Yu Ailin 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

To explore the molecular mechanism of honeybees (Apis mellifera L.) poisoning death by ingestion of Camellia oleifera nectar, feeding experiments were designed to investigate the poisoning effects. Observed the develop ment process of poisoning symptoms, and collected bees at key experimental nodes for transcriptome sequencing analysis. The results showed that the first poisoning symptom was observed within 0.5 h after ingestion of Camellia oleifera nectar, with death occurring as early as 2 h post-ingestion and all honey bees dying within 4 h. There were four stages in the development of poisoning symptoms: normal flying, crawling (abdominal swelling, inability to fly normally), fluttering (inability to crawl normally), and death. Transcriptome sequencing analyzed the causes of poisoning death indicated that honey bee poisoning occurred with the transduction of dietary restriction signals into cells, resulting in the down-regulated expression gene elements that ensure cell health and inhibit apoptosis, including intracellular NAD+-dependent protein deacetylase sirtuin 1, insulin-like growth factor 1, and heat shock proteins. At the same time, the expression of cysteinyl aspartate specific proteinase 7, which protects programmed cell apoptosis, was also downregulated, resulting in the rapid and disorderly deaths of a large number of cells in individuals in a short period of time, and eventually leading to bees fatality.

Transition of Crack Propagation from a Transgranular to an Intergranular Path in an Overaged Al-Zn-Mg-Cu Alloy During Cyclic Loading

Xu Chen,Zhiyi Liu,Peng Xia,Ailin Ning,Sumin Zeng 대한금속·재료학회 2013 METALS AND MATERIALS International Vol.19 No.2

The fatigue crack propagation behavior in the overaged Al-Zn-Mg-Cu alloy was characterized by optical microscopy, scanning electron microscopy, transmission electron microscopy and electron backscatter diffraction. The results revealed that a fatigue crack tended to transgranularly propagate in the near-threshold regime, whereas intergranular crack propagation was dominant at the high ΔK regime. The transition of crack propagation from a transgranular to an intergranular path that occurred in the Paris regime was strongly influenced by the misorientation of adjacent grains and precipitate free zones. In addition, a crystallographic model of crack propagation was proposed to interpret the transition. The fatigue short crack propagation on a single slip plane was responsible for the formation of a transgranular propagation path in the near-threshold regime. The fatigue long crack propagation, which was conducted by a duplex slip mechanism in the Paris regime, led to the formation of fatigue striations. The formation of a zigzag crack in the near-threshold regime was ascribed to the high misorientation of adjacent grains.

A remark on the exponential inequality for negatively associated random variables

Guodong Xing,Shanchao Yang,Ailin Liu,Xiangping Wang 한국통계학회 2009 Journal of the Korean Statistical Society Vol.38 No.1

We give an exponential inequality for negatively associated random variables. The inequality improves the corresponding result which was obtained in [Kim, T. S., & Kim, H. C. (2007). On the exponential inequality for negatively dependent sequence. Korean Mathematical Society. Communications, 22(2), 315321]. We also obtain the convergence rate n-1/2.log n3/2 for the strong law of large numbers, which improves the corresponding one obtained by the authors in the above-cited reference.

Early IL-17A Prevention Rather Than Late IL-17A Neutralization Attenuates Toluene Diisocyanate-Induced Mixed Granulocytic Asthma

Chen Shuyu,Yu Li,Deng Yao,Liu Yuanyuan,Wang Lingwei,Li Difei,Yang Kai,Liu Shengming,Tao Ailin,Chen Rongchang 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.5

Purpose: Interleukin (IL)-17A plays a critical role in the pathogenesis of allergic airway inflammation. Yet, the exact roles of IL-17A in asthma are still controversial. Thus, the aim of this study was to dissect the roles of IL-17A in toluene diisocyanate (TDI)-induced mixed granulocytic asthma and to assess the effects of neutralizing antibody in different effector phases on TDI-induced asthma. Methods: IL-17A functions in allergic airway inflammation were evaluated using mice deficient in IL-17A (Il17a−/−) or IL-17A monoclonal antibody (IL-17A mab, intraperitoneally, 50 μg per mouse, 100 μg per mouse). Moreover, the effects of exogenous recombinant IL (rIL)-17A in vivo (murine rIL-17A, intranasally, 1 μg per mouse) and in vitro (human rIL-17A, 100 ng/mL) were investigated. Results: TDI-induced mixed granulocytic airway inflammation was IL-17A-dependent because airway hyperreactivity, neutrophil and eosinophil infiltration, airway smooth muscle thickness, epithelium injury, dysfunctional T helper (Th) 2 and Th17 responses, granulocytic chemokine production and mucus overproduction were more markedly reduced in the Il17a−/− mice or by IL-17A neutralization during the sensitization phase of wild-type (WT) mice. By contrast, IL-17A neutralization during the antigen-challenge phase aggravated TDI-induced eosinophils recruitment, with markedly elevated Th2 response. In line with this, instillation of rIL-17 during antigen sensitization exacerbated airway inflammation by promoting neutrophils aggregation, while rIL-17A during the antigen-challenge phase protected the mice from TDI-induced airway eosinophilia. Moreover, rIL-17A exerted distinct effects on eosinophil- or neutrophil-related signatures in vitro. Conclusions: Our data demonstrated that IL-17A was required for the initiation of TDI-induced asthma, but functioned as a negative regulator of established allergic inflammation, suggesting that early abrogation of IL-17A signaling, but not late IL-17A neutralization, may prevent the progression of TDI-induced asthma and could be used as a therapeutic strategy for severe asthmatics in clinical settings.

China Consensus Document on Allergy Diagnostics

Chen Hao,Li Jing,Cheng Lei,Gao Zhongshan,Lin Xiaoping,Zhu Rongfei,Yang Lin,Tao Ailin,Hong Haiyu,Tang Wei,Guo Yinshi,Huang Huaiqiu,Sun Jinlyu,Lai He,Lei Cheng,Liu Guanghui,Xiang Li,Chen Zhuanggui,Ma Ha 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.2

The prevalence of allergic diseases has increased dramatically in recent years in China, affecting the quality of life in 40% of the population. The identification of allergens is the key to the diagnosis of allergic diseases. Presently, several methods of allergy diagnostics are available in China, but they have not been standardized. Additionally, cross-sensitization and co-sensitization make allergy diagnostics even more complicated. Based on 4 aspects of allergic disease (mechanism, diagnosis procedures, allergen detection in vivo and in vitro as well as the distribution map of the most important airborne allergens in China) and by referring to the consensus of the European Society of Allergy and Clinical Immunology, the World Allergy Organization, and the important literature on allergy diagnostics in China in recent years, we drafted this consensus of allergy diagnostics with Chinese characteristics. It aims to standardize the diagnostic methods of allergens and provides a reference for health care givers. The current document was prepared by a panel of experts from the main stream of professional allergy associations in China.