PDEs 억제제와 트레드밀 지구성 운동이 파킨슨질환 동물 모델의 운동 기능과 뇌혈관 기능에 미치는 영향

황동주,이웅배,구정훈 한국체육대학교 체육과학연구소 2022 스포츠사이언스 Vol.40 No.1

In our study, we investigated the effect of treadmill endurance exercise(TEE) and PDEs inhibitor(Sildenafil, SIL) on motor function and cerebrovascular function in the mouse model of Parkinson’s disease(PD). To establish PD model, we injected 1-methyl-4-phenyl1,2,3,6-tetrahydropyridine(MPTP, 25mg/kg) and Probenecid(250mg/kg). After that, they are assigned to TEE(10~20m/min, 50min, 5days/week) with SIL administration(7mg/kg/day, 5 times a week, I.P). First, 4 weeks of TEE improve MPTP-induced muscular dysfunction. In addition, the increased level of alpha-synuclein(aSyn) protein induced by MPTP treatment was significantly reduced following TEE and/or SIL administration. In order to validate these results, we examined correlations between muscular function and aSyn protein and found that muscular function was negatively correlated with aSyn protein(r=?0.494, p=0.0025). Moreover, the tight junction-related proteins such as occludin and claudin-5 were significantly decreased by MPTP treatment. However, TEE and/or SIL administration reversed the expression level of occudin protein. Also we examined the correlations between occludin and aSyn protein(r=?0.527, p=0.0011). Taken together, our results demonstrated that TEE and SIL treatment might alleviate the motor deficits, aSyn metabolism, cerebrovascular dysfunction known as one of the pathogenesis of PD.

Heart rate recovery as a critical determinant in weightlifting

황동주,구정훈,김태경,장용철,조준용 한국응용과학기술학회 2023 한국응용과학기술학회지 Vol.40 No.6

Despite its profound impact on athletic performance, the significance of heart rate recovery (HRR) has been insufficiently addressed in the filed of sports science, particularly in the context of weightlifting characterized by brief and intense exertions involving heavy weights. Serving as a valuable indicator of autonomic nervous system and cardiovascular function, HRR assumes a pivotal role in weightlifting. This comprehensive review aims to delineate the specific demands for HRR in weightlifting, shedding light on the often overlooked cardiovascular considerations within training regimes focused on strength and power. The investigation scrutinizes the repercussions of HRR on weightlifting performance, seeking to elucidate how inadequate recovery intervals may result in physiological and psychological consequences. These consequences encompass a distorted perception of effort, disruption of coordination, compromised posture due to irregular breathing, and an overall decline in lifting capacity. The review systematically presents compelling evidence pertaining to heart rate response and recovery patterns during weightlifting, underscoring the critical importance of well-structured rest periods. Furthermore, the review delves into a comprehensive discussion of factors influencing HRR in weightlifting, encompassing variables such as sex, age, cardiovascular function, hydration, nutrition, and psychological aspects. Finally, a key emphasis is placed on the integration of effective HRR techniques into the training regimens of weightlifters, thereby ensuring sustained and optimized performance outcomes.

Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages

황동주,권기천,최동훈,송현근,김길수,정영석,황대연,조준용 한국실험동물학회 2021 Laboratory Animal Research Vol.37 No.3

Background As a laboratory animal resource, the ICR mouse is commonly used in a variety of research fields. However, information on differences in exercise-related characteristics in ICR mice derived from different lineages and the underlying mechanisms remains to be elucidated. In this study, we investigated the intrinsic exercise capacity and a magnitude of response to acute exercise, and sought to identify mechanisms contributing to difference in Korl:ICR (a novel ICR lineage recently established by the National Institute of Food and Drug Safety Evaluation, Korea) and two commercialized ICR lineages derived from different origins (viz., A:ICR mouse from Orient Bio Com, the United States, and B:ICR mouse from Japan SLC Inc., Japan). Results Results showed that despite no significant difference in body weight and weight-proportioned tissue mass of heart and skeletal muscles among groups, the relatively low intrinsic exercise capacity and exaggerated response to acute exercise were identified in B:ICR comparted with Korl:ICR and A:ICR, as reflected by total work and lactate threshold (LT). Also, the mitochondrial efficiency expressed as the complex 1 and complex 1 + 2 respiratory control ratio (RCR) values for cardiac mitochondrial O2 consumption in B:ICR was significantly lower than that in Korl:ICR with higher level of state 2 respiration by glutamate/malate and UCP3 expression in cardiac muscle. Conclusions Taken together, these results indicate that the intrinsic exercise capacity of ICR mouse varies according to lineages, suggesting the role of cardiac mitochondrial coupling efficiency as a possible mechanism that might contribute to differences in the intrinsic exercise capacity and magnitude of response to exercise.

Comparative analysis of restraint stress-induced depressive-like phenotypes in C57BL/6N mice derived from three different sources

황동주,권기천,황대연,서민수,김길수,정영석,조준용 한국실험동물학회 2020 Laboratory Animal Research Vol.36 No.3

C57BL/6NKorl mice are a novel mouse stock recently developed by the National Institute of Food and Drug Safety Evaluation in Korea. Extensive research into the nature of C57BL/6NKorl mice is being conducted. However, there is no scientific evidence for the phenotypic response to restraint stress (RST), a stress paradigm for modeling depressive disorders, in rodents. In this study, we investigated the repeated RST-induced depressive-like phenotypes in C57BL/6 N mouse substrains (viz., C57BL/6NKorl mice from Korea, C57BL/6NA mice from the United States, and C57BL/6NB mice from Japan) obtained from different sources. The results showed that C57BL/6 N mice derived from various sources exposed to repeated RST resulted in depressive-like phenotypes reflected by a similar degree of behavioral modification and susceptibility to oxidative stress in a duration-dependent manner, except for the distinctive features (increased body weight (BW) and tolerance to the suppression of BW gain by exposure to repeated RST) in C57BL/6NKorl mice. Taken together, the duration-dependent alteration in depressive-like phenotypes by repeated exposure to RST observed in this study may provide valuable insights into the nature of C57BL/6NKorl mice as an alternative animal resource for better understanding of the etiology of depressive disorders and the mechanisms of antidepressant actions.

수학적 상황과 비수학적 상황에서의 확산적 사고의 관계 연구 - TTCT의 도형검사와 MCPSAT를 중심으로 -

황동주,이강섭,서종진,Hwang, Dong-Jou,Lee, Kang-Sup,Seo, Jong-Jin The Korean Society for the Gifted 2005 영재교육연구 Vol.15 No.2

본 연구의 목적은 수학적 상황에서의 확산적 사고와 비수학적 상황에서의 확산적 사고의 관계를 조사하기 위하여 중학교 2학년 학생 215명을 대상으로 검사를 실시하여 자료를 분석하였다. 자료 분석은 빈도, 퍼센트, t-검증과 상관 분석을 사용하였다. 본 연구의 결과는 첫 번째, 수학 영재 학생이 일반 학생보다 수학적 상황에서의 확산적 사고(MCPSAT)와 비 수학적 상황에서의 확산적 사고(TTCT)는 통계적으로 유의미하게 높은 점수를 받았다. 두 번째, 여학생이 남학생보다 비 수학적 상황에서의 확산적 사고(TTCT)에서 제목의 추상성을 제외하고 모든 요소에서 통계적으로 유의미하게 높은 점수를 받았다. 세 번째, 남학생이 여학생보다 수학적 상황에서의 확산적 사고에서 유창성과 융통성은 평균이 높게 나타나고 있으나 통계적으로는 유의미하지 않고 여학생이 남학생보다 수학적 상황에서의 확산적 사고에서 독창성의 평균이 높게 나타나고 있으며 통계적으로 유의미하게 나타나고 있다. 네 번째, 수학적 상황과 비 수학적 상황에서의 확산적 사고 점수사이의 상관관계는 통계적으로 유의미하게 나타나고 있으며 중학생 전체에서는 r=.41(p<.05)이고 r=.21에서 r=.56까지 분포하고 있으며 일반 학생은 r=.27(p<.05)이고 r =.07에서 r=.27까지 분포하고 있다. 다섯 번째로 수학 영재학생의 경우는 수학적 상황과 비 수학적 상황에서의 확산적 사고 점수사이의 상관관계는 r=.11이며 통계적으로 유의미하지 않게 나타나고 있다. 이 결과는 수학 영재학생의 경우 수학적 상황과 비 수학적 상황에서의 확산적 사고 점수사이의 상관관계는 거의 0에 가깝다고 할 수 있다. 이것은 수학적 상황에서의 확산적 사고능력은 비 수학적인 상황에서의 확산적 사고 조합된 능력이 아니라 다른 특별한 능력이라고 볼 수 있다. 그러나 본 연구에서 수학 영재 학생들의 사례수가 적어서 수학 영재 학생의 수학적 상황과 비 수학적 상황에서의 확산적 사고 점수 사이의 상관관계가 있다는 주장을 일반화하기에는 충분치 않을 수 있다는 제한점을 가지고 있다. We examined the relations between the score of the divergent thinking in mathematical (Mathematical Creative Problem Solving Ability Test; MCPSAT: Lee etc. 2003) and non-mathematical situations (Torrance Test of Creative Thinking Figural A; TTCT: adapted for Korea by Kim, 1999). Subjects in this study were 213 eighth grade students(129 males and 84 females). In the analysis of data, frequencies, percentiles, t-test and correlation analysis were used. The results of the study are summarized as follows; First, mathematically gifted students showed statistically significantly higher scores on the score of the divergent thinking in mathematical and non-mathematical situations than regular students. Second, female showed statistically significantly higher scores on the score of the divergent thinking in mathematical and non-mathematical situations than males. Third, there was statistically significant relationship between the score of the divergent thinking in mathematical and non-mathematical situations for middle students was r=.41 (p<.05) and regular students was r=.27 (p<.05). A test of statistical significance was conducted to test hypothesis. Fourth, the correlation between the score of the divergent thinking in mathematical and non-mathematical situations for mathematically gifted students was r=.11. There was no statistically significant relationship between the score of the divergent thinking in mathematical and non-mathematical situations for mathematically gifted students. These results reveal little correlation between the scores of the divergent thinking in mathematical and non-mathematical situations in both mathematically gifted students. Also but for the group of students of relatively mathematically gifted students it was found that the correlations between divergent thinking in mathematical and non-mathematical situations was near zero. This suggests that divergent thinking ability in mathematical situations may be a specific ability and not just a combination of divergent thinking ability in non-mathematical situations. But the limitations of this study as following: The sample size in this study was too few to generalize that there was a relation between the divergent thinking of mathematically gifted students in mathematical situation and non-mathematical situation.

고정, 임의시간 강우량 선택에 따른 농경지 배수 영향 분석

황동주,김병규,심좌근,Hwang, Dong Joo,Kim, Byoung Gyu,Shim, Jwa Keun 한국관개배수위원회 2012 한국관개배수논문집 Vol.19 No.1

Recently, it has been increased disaster of crops and agricultural facilities with climate change such as regional storm, typhoon. However agricultural facilities have unsafe design criteria of improving drainage corresponding to this change. This study has analyzed the impact that inundation area and magnitude of drainage-facility is decided based on fixed- and unfixed-duration precipitation by applying revised design criteria of drainage for climate change. The result was shown that 1-day and 2-days rainfall for 20-years return period has increased about 11.4%, 4.4% respectively by changing fixed- to unfixed duration. And the increase rate of design flood was 15.0%. The result was also shown that Inundation area was enlarged by 6.6% as well as increased inundation duration under same basic condition in designed rainfall between fixed- and unfixed-duration. According to the analysis, it is necessary for pump capacity in unfixed-duration to be increased by 70% for same effect with fixed-duration. Therefore, when computing method of probability precipitation is changed from fixed one to unfixed-duration by applying revised design criteria, there seems to be improving effect in drainage design. Because 1440-minutes rainfall for 20-years return period with unfixed-duration is more effective than 1-day rainfall for 30-years return period with fixed-duration. By applying unfixed-duration rainfall, capacity of drainage facilities need to be expanded to achieve the same effects (Inundation depth & duration) with fixed-duration rainfall. Further study is required for considering each condition of climate, topography and drainage by applying revised design criteria.

ERIC 검색을 통한 미국의 수학영재교육 프로그램 분석

황동주,홍지창,서종진 한국수학교육학회 2002 수학교육 학술지 Vol.7 No.-

Comparative analysis of dose-dependent neurotoxic response to 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine in C57BL/6 N mice derived from three different sources

황동주,권기천,송현근,김길수,정영석,황대연,조준용 한국실험동물학회 2019 Laboratory Animal Research Vol.35 No.2

MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine is commonly used to induce nigrostriatal defects to induce parkinsonism and/or parkinsonian syndrome, to replicate the lesions seen in Parkinson’s disease (PD), with use in numerous PD models in mice. It has been suggested that various biological characteristics including strain could result in differing mortality rates, sensitivity to MPTP administration, and reproducibility of lesions in mice, but there is no evidence on the sensitivity of C57BL/6 mice from different origins to MPTP and its associated pathological lesions. In this study, we investigated the magnitude of the dose-dependent response to acute MPTP administration in C57BL/ 6NKorl mice and two commercialized C57BL/6 stocks derived from the United States and Japan. We measured biological features (body weight, temperature, and composition), nigrostriatal neurotoxic responses (dopamine levels, tyrosine hydroxylase enzymes, and protein carbonylation) and motor function. In results, the three different C57BL/6 stocks exhibited similar overall neurotoxic response and locomotor impairment which increased in a dose-dependent manner with acute MPTP administration (10 mg/kg, 20 mg/kg, and 30 mg/kg, all with external heat support), although some of these differences were not significant. In conclusion, this study provides scientific evidence that C57BL/6NKorl mice can be used as an alternative animal model for practical and targeted PD research.

중학교 1학년 수학 영재학생과 일반 학생의 수학 문제해결과 문제설정 능력의 차이 비교

황동주 한국학교수학회 2006 韓國學校數學會論文集 Vol.9 No.3

본 연구에서는 수학 문제해결력 검사 도구를 개발하여 수학 영재와 일반 학생의 수학 문제 해결력의 차이를 조사하였다. 수학 문제 해결력 검사 도구는 10문항으로 신뢰도, 타당도, 변별도가 높은 도구이다. 연구 대상은 중학교 1학년 168명의 일반 학생과 150명의 수학 영재 학생으로 총 318명을 대상으로 하였다. 본 연구 결과분석은 빈도, t-검증과 을 사용하였다. 결과는 수학 영재의 특성이 수학 문제 해결 능력뿐만 아니라 수학 설정 능력도 수학 영재의 특성이라고 볼 수 있다.

수학 영재를 위한 행동 특성 검사도구 개발

황동주 한국학교수학회 2006 韓國學校數學會論文集 Vol.9 No.3

본 연구는 수학 영재의 행동특성 검사 도구를 개발하는데 목적이 있다. 연구는 문헌 연구를 통해 수학 영재의 행동 특성을 추출하여 유목화한 후 측정변인으로 규정하였으며, 각각의 측정변인별로 문항을 개발하여 예비검사 과정을 통하여 최종적으로 51개 문항으로 구성된 검사 도구를 개발하였다. 기존에 영재교육을 받은 학생과 표준화된 수학 창의적 문제해결력에서 상의 10%와 교사 지명 학생 포함하여 424명을 연구 대상으로 본 검사를 실시하였다. 검사도구의 신뢰도와 타당도를 검증한 결과 높은 신뢰도(.95)를 확보하였으며 Rash 1모수 모형을 이용하여 내적 타당도를 검증하였다. 주성분 요인추출방법으로 요인을 추출하여 Varimax 방법으로 직각회전을 한 구인타당도 검증에서 일반적인 수학정신 능력 요인, 수학적 능력 요인, 정보 수집과 처리 능력 요인과 수학적 성향 요인이 추출되었다 따라서 본 연구에서 개발한 수학 영재 행동 특성 검사 도구는 신뢰도와 타당도가 양호하게 검증되었다고 볼 수 있다.