핵산증폭시험을 이용한 혈장분획물질에서 HCV RNA 검출

홍승희,Hong, Seung-Hee 한국미생물학회 2008 미생물학회지 Vol.44 No.4

HCV는 HIV등과 함께 수혈이나 혈장된 획물질을 통하여 감염되는 주요 바이러스이다. 주로 혈액이나 혈장에서 HCV에 대한 항체를 검출함으로서 HCV의 감염을 방지하고 있다. 그러나 바이러스에 감염되었으나 항체가 생성되기 이전이나 항체의 양이 적은 경우에는 HCV의 검출이 어렵다. 따라서 핵산중폭시험(nucleic acid amplification tests, NAT)을 이용한 HCV 유전자를 검출하려는 시도들이 진행되고 있다. 이 연구의 목적은 혈장분획물질에서 HCV RNA를 검출할 수 있는 핵산증폭시험 방법을 개발하는 것이다. 5종류의 PCR primer를선별하여 실험에 이용하였다. 혈장분획물질의 HCV RNA 추출에는 컬럼 방법을 이용하는 것이 유용한 것으로나타났다. 핵산중폭시험의 결합 온도는 $48^{\circ}C$가 가장 적절한 것으로 나타났다. 또한 2차 PCR의 경우, 1차 PCR 산물 $1{\mu}l$와 30 pmol의 primer즐 사용하였을 때 높은 민감도와 특이성을 보이는 것을 알 수 있었다. 혈장 분획물질에 HCV를 주입하여 혈장중폭시험을 수행한 결과, 100 IU/ml까지 검출 할 수 있었다. 한편 근육주사용항체(IMIG)의 경우 핵산중폭시험을 통한 검출한계는 100IU/ml로 COBAS amplicor HCV2.0의 500 IU/ml 이상의 검출한계보다 민감도가 더 높은 것으로 나타났다. 이러한 결과들로 보아 본 실험에 이용된 핵산증폭시험이 혈장분획물질에서 HCV RNA를 검출하는데 유용한 방법으로 사용될 수 있을 것으로 생각된다. HCV is transmitted via various plasma derived products. Current methods to detect hepatitis C virus (HCV) are based on its antibody detection in the donated blood and plasma. Viral contamination can potentially escape such detection during the window period of infection, when no antibody is present or the level of antibody is too low to detect. It is trying to application of nucleic acid amplification tests (NAT) for the direct detection of HCV. The objective of this study was to develop a reliable NAT for the HCV RNA detection from plasma-derived products. The most useful primers was selected for NAT among 5 sets of primers. We have also found that QIAamp viral RNA isolation kit was the most efficient for HCV RNA isolation. The highest sensitivity and specificity was appeared in $48^{\circ}C$ annealing temperature and 30 pmol of primers. With a spiking of HCV to albumin, immunoglobulins and coagulation factors, NAT can detect up to 100 IU/ml. Meanwhile, COBAS amplicor HCV 2.0 afforded a lower sensitivity in high concentrated intramuscular immunoglobulins to below 500 IU/ml. Our results suggested that NAT appears to be a highly sensitive and specific method for HCV RNA detection in plasma-derived products.

기치유가 자율신경계의 조절능력에 미치는 영향

홍승희,오평일,이한춘,이미자,Hong, Seung-Hui,Oh, Pyung-Il,Lee, Han-Chun,Lee, Mi-Ja 한국전자통신학회 2013 한국전자통신학회 논문지 Vol.8 No.7

기치유가 자율신경계의 조절 능력에 미치는 영향을 알아보기 위하여 19명을 대상으로 '생활이완 시행'과 '기치유 시술' 전 후의 체감 변화와 심박변이도의 신체저항도, 평균심박수, 주파수 영역분석의 차이를 조사 및 측정하였다. 대상자들은 '생활이완 시행'과 '기치유 시술' 전 후의 체감 변화 설문에서 각각 약 8.5%와 약 23.3%의 스트레스 감소 변화율을 나타내어 '기치유 시술'이 스트레스 완화 및 감소에 긍정적인 영향을 미친 것으로 판단되었다. 심박 변이도의 신체저항도, 평균심박수, 주파수 영역 분석을 조사한 결과에서 '생활이완 시행'은 유의한 차이가 없었으나 '기치유 시술'에서는 6개 항목에서 유의한 차이를 나타내어 '기치유 시술'이 자율신경계의 조절 능력에 영향을 미쳐 스트레스 저항도를 향상시키고 스트레스 감소 및 완화에 조력할 수 있음이 입증되어 보완 대체 요법으로서 가치가 있다고 판단된다. This paper demonstrates that 'Qi Energy Healing' effects on elevation of capabilities in regards with the autonomic nervous system which prevents from the stress. Nineteen participants were assigned into 'Qi Energy Healing' and the 'Ordinary Relaxation' condition. Participants were examined to see the difference of frequency domain analysis, heart rate tachogram, physical resistance through the heart rate variability. The 'Qi Energy Healing' is significantly more likely to influence on reducing and diminishing the stress. 'Qi Energy Healing' treatment showed clear distinction on 6 fact. Our findings established the link between effectiveness of 'Qi Energy Healing' and the autonomic nervous system.

한국 청소년의 패스트푸드 섭취실태 및 관련요인 - 제16차 청소년건강행태온라인조사 이용 -

홍승희,Hong, Seung Hee 한국식품영양학회 2022 韓國食品營養學會誌 Vol.35 No.3

The purpose of study was to examine the factors influencing fast food consumption in Korean adolescents. The analysis was conducted using cross sectional study data from the 16^th Korea Youth Risk Behavior Web-based Survey in 2020. A total 54,948 middle and high school students participated in this study. The subjects in the analysis were 28,353 males and 26,595 females, 28,961 middle school and 25,987 high school students. In total, 56.6% Korean adolescents consumed fast food once or twice weekly and 25.4% consumed fast food more than three times weekly. Logistic regression analysis revealed that fast food consumption was significantly associated with dietary behavior such as lower breakfast intake (OR: 0.930, 95%CI: 0.891~0.970, p<0.001), higher soda drinks consumption (OR: 2.563, 95%CI: 2.452~2.678, p<0.001), and higher sweet drinks consumption (OR: 1.898, 95%CI: 1.818~1.982, p<0.001). For psychological and health behavior factors, fast food consumption was also significantly associated with higher perceived stress (OR: 1.239, 95%CI: 1.163-1.321, p<0.001), higher smoking (OR: 1.300, 95%CI: 1.164~1.453, p<0.001), higher drinking (OR: 1.193, 95%CI: 1.112~1.280, p<0.001), higher depression experience, higher loneliness experience, and lower subjective health, In conclusion, fast food consumption in Korean adolescents was associated with undesirable dietary habits and psychological and health behavior, suggesting that appropriate education programs are necessary to reduce such behavior.

서울 일부지역 초등학생의 식습관 및 간식과 군것질 섭취실태

홍승희,이보라,박영심,Hong, Seung-Hee,Lee, Bo-Ra,Park, Young-Sim 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.1

In this study, we investigated the dietary habits, snacks, and self-purchasing snacks (SPS) intake behaviors of 519 elementary school students (boys=239, girls=280). Obesity was significantly higher (p<0.05) in boys (24.8%) than in girls (14.7%) and the proportion of underweight subjects was higher compared to normal or other weight groups for both the boys and the girls. There were 7.5% of the subjects in the group that always skipped breakfast, and the main reason of skipping breakfast was insufficient time (51.9%). The snack intake frequency was once or twice per week for 23.1% of the subjects and three or four times per week for 25.1%. The SPS intake frequency was the subjects zero for 35.6% of the highest level of the responders, while 6.8% of the respondents took more than once SPS per day. 59.6% of the respondents consumed SPS due to hunger while 15.0% consumed SPS out of boredom. The SPS was purchased from supermarkets in 34.5% of the cases, from convenience stores in 24.1% and from snack corners in 20.0% of cases or from a store near school in 14.5% of the cases. Analysis of SPS behaviors according to obesity index showed that parent's opinion of 'permission to buy SPS as needed' had a significant effect in 64.5% over weight subjects compared to only 53.7% in underweight groups. The subjects who used more than 3/4 of their pocket money to buy SPS was higher in the overweight groups (16.4%) than in the underweight groups (7.0%) and normal weight groups (9.8%). The favorite snacks and SPS were milk and yogurt for 45.7% of the subjects, fruits for 42.7%, ice cream for 26.4%, fruit juices for 23.8%, sweet stuff for 16.4%, frozen dessert for 8.9%, and chocolate or candy for 8.1% in descending order. The intake frequency of milk, yogurt, and fruit juices was higher in the underweight groups, but the intake frequency of sweet stuff, frozen dessert, and chocolate or candy was higher in over weight groups. The intake of frozen dessert was more than four times higher in the overweight groups than in the underweight groups. In conclusion, dietary habits, snacks, and SPS intake behaviors were similar between the boys and the girls and obesity groups, but most students appeared to have a high preference for intake snacks and SPS. Therefore, education for appropriate snacks intake habits will be beneficial for improving their dietary habits and health.

국민참여재판법 개정안의 개선방안

홍승희(Hong, Seung-Hee) 한국형사법무정책연구원 2014 형사정책연구 Vol.99 No.-

의사소통장애아동 부모교육 프로그램의 모형개발을 위한 요구도 조사 연구

홍승희(Seung Hee Hong),이은경(Eun Kyoung Lee) 한국언어치료학회 2017 言語治療硏究 Vol.26 No.4

Purpose: This study intends to provide basic data for future parent education programs by investigating the types and contents of parental education by questioning parents who have children with communication disabilities. Method: For this, questionnaires were distributed to 289 parents of children with communication disabilities This study targeted speech disorders, language disorders, hearing impairment, social communication disorder, and other disorders, by sub-types of children with communication disorders. The questionnaire for this study included three sections: basic personal information, parent education experience, and the need for parent education programs for language therapy by referring to previous studies and literature. Statistical analysis was performed through frequency analysis, variance analysis, and Scheffé test.Results: The results of the present study were as follows: The need for parent education programs depending on the type of child with disability was higher than average in the order of: communication, overall area, language, auditory, and spoken areas. These findings suggest that parents of children with disabilities recognize the necessity of these areas in language therapy and want them to be included in the content of parent education programs. By analyzing the need for parent education programs according to the types of disabilities, basic data for the configuration and model development of parent education programs for children with communication disorders was provided. Conclusions: There is a high possibility that the results of this study can be used as a basic data to reflect the actual needs of parents in language therapy processes in the future.

대학생에서 야식의 섭취가 영양소 섭취 상태에 미치는 영향

홍승희(Seung-Hee Hong),연지영(Jee-Young Yeon),배윤정(Yun-Jung Bae) 동아시아식생활학회 2013 동아시아식생활학회지 Vol.23 No.3

이혼시 재산분할에 관한 미국의 법률과 판례 분석

홍승희(Hong Seung Hee),손종학(Sohn Jong Hak) 원광대학교 법학연구소 2021 圓光法學 Vol.37 No.1

민법 제839조의2(재산분할청구권)는 재산분할에 관하여 협의가 되지 아니하거나 협의할 수 없는 때에는 가정법원이 당사자의 청구에 의하여 당사자 쌍방의 협력으로 이룩한 재산의 액수 기타 사정을 참작하여 분할의 액수와 방법을 정한다고 하여, 재산분할 대상을 ‘당사자 쌍방의 협력으로 이룩한 재산’으로, 재산분할 방법 및 비율은 ‘가정법원이 재산의 액수 기타 사정을 참작하여 분할의 액수와 방법을 정한다’고 규정한다. 이혼시 매우 중요한 부분인 당사자 쌍방의 협력으로 이룩한 재산의 구체적인 범위와 재산분할 방법 및 비율이 사실심 법원의 재량에 달려있는 실정이다. 2006년에 이혼시 재산분할과 관련하여, 법무부가 마련한 정부안과 한명숙 의원안, 이계경 의원안 등이 발의되어 재산분할은 균등분할을 원칙으로 하고 구체적인 경우에 타당성을 결여하지 않도록 법원의 재량에 의하여 분할비율을 조정할 수 있도록 하였다. 비록 민법개정으로 이어지지 않았지만, 이혼시 재산의 균등분할 원칙이 헌법상 양성평등의 이념에 부합하고, 미국의 많은 주(州)들이 법률에서 재산의 균등분할 원칙을 천명하거나, 판례를 통하여 균등분할이 형평(equitable) 분할이라고 판시하고 있으므로, 우리 나라도 민법 개정을 통하여 이혼시 재산의 균등분할 원칙을 명시하는 것이 바람직하다고 생각한다. 재산분할 비율과 관련하여 고려할 사항에 대해서도 현행 법문인 “재산의 액수 기타 사정을 참작하여”는 너무 추상적이므로, 미국과 같이 나이, 혼인기간, 기여도 등은 물론 가사 혹은 양육을 위한 교육･취업 기회 포기, 양육권자에게 가족의 거주지 및 가재도구를 점유･사용하게 할 필요성, 사업(business)･기업(corporation) 혹은 전문성(profession)에 대한 자산이나 이익의 평가 가능성 혹은 곤란성과 일방 배우자의 청구나 간섭(interference)으로부터 자유롭게 그러한 자산이나 이익을 보유하는 것이 경제적으로 바람직한지 여부, 결혼기간 누렸던 것과 비슷한 생활수준으로 당사자가 독립하여 지낼 수 있도록 하기에 충분한 교육이나 훈련을 받게 하는데 필요한 시간과 비용 등 재산분할 비율산정시 고려할 요소를 법문에 명시하는 것이 구체적 사실관계를 반영하여 공평한 재산분할을 하는데 도움이 될 것이다. 또한 재산분할의 대상 및 범위에 대해서도, 법원의 판단에만 맡길 것이 아니라, 재산분할의 대상이 되는 공동재산(community property) 혹은 부부재산 (marital property), 그리고 원칙적으로 재산분할의 대상이 되지 않은 특유재산(separate property) 및 고유재산(individual property)의 개념 및 범위 그리고 예외 조항을 신설하는 것이 법률의 명확성과 예측가능성을 높일 수 있을 것으로 생각한다. Article 839-2 of the Civil Act in Korea (the right to claim division of property) stipulates that when there is no consultation or consultation cannot be reached on the division of property, the amount and method of division shall be determined by the family court at the request of the parties, taking into account the amount of property achieved by the cooperation of the parties and other circumstances. The subject of property division shall be the property achieved by the cooperation of both parties, and the amount and method of division shall be determined by the family court in consideration of the amount of property and other circumstances. The specific scope of property achieved by cooperation between the parties, method and proportion of property division, which is a very important part of the divorce, depend on the discretion of the court. Regarding matters to be considered in relation to the ratio of property division, the current law, Considering the amount of property and other circumstances, is too abstract. In 2006, the Ministry of Justice prepared a bill on the division of property in case of divorce. National Assembly member Han Myung Sook, Lee Kye Kyung also proposed a bill that in principle, the division of property should be made equally and the division ratio could be adjusted at the discretion of the court to ensure that it did not lack validity in specific cases. Although it did not lead to a revision of civil law, the principle of equal division of property upon divorce conforms to the ideology of gender equality in the Constitution. Also, as many states in the United States proclaim the principle of equal division of property in law, and many cases suggests that equal division is an equity division, I think it is desirable for our country to specify the principle of equal division of property in case of divorce through the revision of civil law Regarding matters to be considered about the ratio of property division, the current law, in consideration of the amount of property and other circumstances, is too abstract. As in the United States, to state in the law what factors to consider in calculating the ratio of property division will help to make a fair division of property by reflecting specific facts. The specific factors will include not only age, the length of the marriage, degree of contribution but also abandonment of educational and employment opportunities for household(homemaking) or child support(child care service), the need of a custodial parent to occupy or own the marital residence and to use or own its household effects, the impossibility or difficulty of evaluating any component asset or any interest in a business, corporation or profession, and the economic desirability of retaining such asset or interest intact and free from any claim or interference by the other party, the time and expense necessary to acquire sufficient education or training to enable the party to become self-supporting at a standard of living reasonably comparable to that enjoyed during the marriage. In addition, the subject and scope of property division should not be left to the court s judgment, but civil law will have to specify the concept and scope of community property or marital property which is subject to property division, the concept and scope and exceptions of separate property and individual property which is not subject to property division in principle.

먹방 및 쿡방 시청이 청소년의 식습관 및 정신 건강에 미치는 영향: 제18차 청소년건강행태 조사를 이용한 단면연구

홍승희(Seung-Hee Hong) 대한지역사회영양학회 2024 대한지역사회영양학회지 Vol.29 No.2

Objectives: This study aimed to investigate the association between how often Korean adolescents watch Mukbang and Cookbang videos and their dietary habits. Methods: Data from the 18th Korea Youth Risk Behavior Survey conducted in 2022 was analyzed for this study. The study included 51,850 middle and high school students and assessed various aspects, including demographics, frequency of watching Mukbang and Cookbang videos per week, dietary habits, health behaviors, and mental health factors. Results: Nearly a third (29.3%) of Korean adolescents watched Mukbang and Cookbang videos one to four times a week, while 13.5% watched them more than five times weekly. Females, those with lower academic achievement, and those from lower socioeconomic backgrounds were significantly more likely to be frequent viewers (P < 0.001). Increased viewing frequency was associated with poorer dietary habits. Adolescents who watched more frequently were less likely to eat breakfast and consume fruits and milk, while their consumption of fast food, high-caffeine drinks, sugary drinks, and late-night snacks increased (P < 0.001). Higher viewing frequency correlated with increased feelings of stress, depression, and loneliness (P < 0.001). Logistic regression analysis confirmed these associations. More frequent viewers were significantly less likely to eat breakfast (odds ratio (OR), 0.63; 95% confidence interval (CI), 0.58–0.68), and more likely to consume fast food (OR, 1.85; 95% CI, 1.69–2.02), high-caffeine drinks (OR, 1.43; 95% CI, 1.30–1.56), sugary drinks (OR, 1.54; 95% CI, 1.41–1.67), and late-night snacks (OR, 1.37; 95% CI, 1.25–1.51). Conclusions: Our findings suggest that frequent exposure to Mukbang and Cookbang content is linked to unhealthy dietary habits in adolescents. Educational programs may be necessary to mitigate the potential for these videos to negatively influence dietary choices.

온라인 서비스 플랫폼 브랜드 개발을 위한 비대면 환경에서의 Co-creation 사례 연구

홍승희(Seunghee Hong),이정선(Jungsun Lee) 한국디자인학회 2021 한국디자인학회 학술발표대회 논문집 Vol.2021 No.5