바이오피드백을 이용하여 측정한 불면증 환자의 정신생리적 특징

허성영,이진성,김성곤,김지훈,정우영,Huh, Sung-Young,Lee, Jin-Seong,Kim, Sung-Gon,Kim, Ji-Hoon,Jung, Woo-Young 대한수면의학회 2015 수면·정신생리 Vol.22 No.2

목 적 : 불면증은 여러 가지 신체질환, 정신질환에 공이환 된 경우가 많아 일차 진료에서 가장 흔한 문제 중 하나이다. 정신생리적 과각성이 병태생리에서 중요하다고 알려져 있다. 이 연구에서는 바이오피드백 기기를 이용한 스트레스 반응 검사로 일차성 불면증과 정상인 간에 생리신호 특성을 비교하였다. 방 법 : 일차성 불면증 환자 80명(남자 35, 여자 45명, $49.71{\pm}12.91$세)과 정상인 101명(남자 64, 여자 37명, $27.65{\pm}2.77$세)에게 바이오피드백 기기를 이용하여 스트레스 반응 검사를 하였다. 스트레스 반응 검사는 기저, 스트레스 1, 회복 1, 스트레스 2, 회복 2단계의 다섯 단계로 하였다. 각 단계에서 근전도(EMG), 심장박동수(heart rate), 피부전도(skin conductance), 피부체온(skin temperature), 그리고 호흡수(respiratory rate)를 각각 2분 동안 기록하여 평균값을 산출하였다. 측정값들의 양 군 사이 비교에는 독립 t-검정, 이전 단계와 비교한 측정값의 변화는 대응표본 t-검정으로 분석하였다(양측검정, p < 0.05). 결 과 : 불면증 환자는 정상인에 비해 5단계 스트레스 반응 검사 모두에서 전두근이 더 긴장해 있었다(기저 : $7.72{\pm}3.88{\mu}V$ vs. $4.89{\pm}1.73{\mu}V$, t = -6.06, p < 0.001 ; 스트레스 1 : $10.29{\pm}5.16{\mu}V$ vs. $6.63{\pm}2.48{\mu}V$, t = -5.84, p < 0.001 ; 회복 1 : $7.87{\pm}3.86{\mu}V$ vs. $5.17{\pm}2.17{\mu}V$, t = -5.61, p < 0.001 ; 스트레스 2 : $10.22{\pm}6.07{\mu}V$ vs. $6.98{\pm}2.98{\mu}V$, t = -4.37, p < 0.001 ; 회복 2 : $7.88{\pm}4.25{\mu}V$ vs. $5.17{\pm}1.99{\mu}V$, t = -5.27, p < 0.001 ). 정상인은 불면증 환자와 비교하여 각 단계에 따른 심장박동수의 변화 정도가 더 컸다(스트레스 1-기저 : $6.48{\pm}0.59$ vs. $3.77{\pm}0.59$, t = 3.22, p = 0.002 ; 회복 1-스트레스 1 : $-5.36{\pm}0.0.59$ vs. $-3.16{\pm}0.47$, t = 2.91, p = 0.004 ; 스트레스 2-회복 1 : $8.45{\pm}0.61$ vs. $4.03{\pm}0.47$, t = 5.72, p < 0.001 ; 회복 2-스트레스 2 : $-8.56{\pm}0.65$ vs. $-4.02{\pm}0.51$, t = -5.31, p < 0.001). 결 론 : 일차성 불면증 환자는 정상인에 비해 전두근 근전도 측정값이 전반적으로 증가되었고, 심장박동수의 검사 단계별 변화 정도가 작았다. 이는 불면증 환자의 자율신경계가 정상인에 비해 과각성 상태에 있음을 시사한다. Background and Objectives: Insomnia is the most prevalent sleep disorder in the general population and is considered to be a disorder of hyperarousal. The aim of this study was to measure the psychophysiological responses in insomnia patients using a biofeedback system, and to compare them with results from normal healthy subjects. Materials and Methods: Eighty patients with primary insomnia (35 males and 45 females, average age $49.71{\pm}12.91years$) and 101 normal healthy controls (64 males and 37 females, average age $27.65{\pm}2.77$) participated in this study. Electromyography (EMG), heart rate (HR), skin conductance (SC), skin temperature (ST), and respiratory rate (RR) were recorded using a biofeedback system during 5 phases (baseline, stress 1, recovery 1, stress 2, recovery 2) of a stress reactivity test, and average values were calculated. Difference in values between the two groups in each corresponding phase was analyzed with independent t-test, and change in values across phases of the stress reactivity test was analyzed with paired t-test (all two-tailed, p<0.05). Results: Compared to normal controls, insomnia patients had higher EMG in all 5 phases (baseline : $7.72{\pm}3.88{\mu}V$ vs. $4.89{\pm}1.73{\mu}V$, t = -6.06, p<0.001 ; stress 1 : $10.29{\pm}5.16{\mu}V$ vs. $6.63{\pm}2.48{\mu}V$, t = -5.84, p<0.001 ; recovery 1 : $7.87{\pm}3.86{\mu}V$ vs. $5.17{\pm}2.17{\mu}V$, t = -5.61, p<0.001 ; stress 2 : $10.22{\pm}6.07{\mu}V$ vs. $6.98{\pm}2.98{\mu}V$, t = -4.37, p<0.001 ; recovery 2 : $7.88{\pm}4.25{\mu}V$ vs. $5.17{\pm}1.99{\mu}V$, t = -5.27, p<0.001). Change in heart rate across phases of the stress reactivity test were higher in normal controls than in insomnia patients (stress 1-baseline : $6.48{\pm}0.59$ vs. $3.77{\pm}0.59$, t = 3.22, p = 0.002 ; recovery 1- stress 1 : $-5.36{\pm}0.0.59$ vs. $-3.16{\pm}0.47$, t = 2.91, p = 0.004 ; stress 2-recovery 1 : $8.45{\pm}0.61$ vs. $4.03{\pm}0.47$, t = 5.72, p<0.001 ; recovery 2-stress 2 : $-8.56{\pm}0.65$ vs. $4.02{\pm}0.51$, t = -5.31, p<0.001). Conclusion: Psychophysiological profiles of insomnia patients in a stress reactivity test were different from those of normal healthy controls. These results suggest that the sympathetic nervous system is more highly activated in insomnia patients.

The pepsinolytic hydrolysate from Johnius belengerii frame inhibited LPS-stimulated production of pro-inflammatory mediators via the inactivating of JNK and NF-κB pathways in RAW 264.7 macrophages

허성영,고석천,정원교 한국수산과학회 2018 Fisheries and Aquatic Sciences Vol.21 No.2

The objective of this study was to investigate the anti-inflammatory effects of the pepsinolytic hydrolysate from the fish frame, Johnius belengerii, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The J. belengerii frame hydrolysate (JFH) significantly suppressed nitric oxide (NO) secretion on LPS-stimulated RAW264.7 macrophages. Moreover, the JFH markedly inhibited the levels of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, the LPS-stimulated mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was downregulated when cells were cultured with the JFH. The JFH significantly reduced the phosphorylation of c-Jun N-terminal kinase (JNK) and the translocation of nuclear factor-kappa B (NF-κB) in macrophages. As the result, the JFH has the potential antiinflammatory activity via blocking the JNK and NF-κB signal pathways.

연골 조직재생을 위한 해양 천연고분자 소재의 의공학적 응용

허성영,정원교 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.14 No.3

Recently, many researchers paid a higher attention on tissue engineering approach that provide physiological and biological substitute to replace tissue loss due to some disease, congenital abnormalities or trauma. For that reason, the development of scaffolding substance for tissue engineering applications has focused on the designing of biomimetic materials that are capable of eliciting specific cellular responses mediated by biomolecular recognition, which can be manipulated by altering design parameters of the material. Marine derived polysaccharide such as alginate, chitosan and hyaluronic acid has demonstrated high biocompatibility, biodegradability, non-toxicity and low chronic inflammatory response. Therefore, these materials can applicable tissue engineering application. Among them, alginate is a well-known biomaterial obtained from brown macroalgae and is widely used in biomedical, drug delivery and tissue engineering application. Moreover, since marine organism derived materials have not yet been reported with bovine spongiform encephalopathy, transmissible spongiform encephalopathy and foot and mouth disease compared to land animals. In light of the various proven, this review focuses on tissue regenerative properties of natural polysaccharides derived from marine organisms for cartilage tissue regeneration application.

연골 조직재생을 위한 해양 천연고분자 소재의 의공학적 응용

허성영 ( Seong Yeong Heo ),정원교 ( Won Kyo Jung ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.2

Recently, many researchers paid a higher attention on tissue engineering approach that provide physiological and biological substitute to replace tissue loss due to some disease, congenital abnormalities or trauma. For that reason, the development of scaffolding substance for tissue engineering applications has focused on the designing of biomimetic materials that are capable of eliciting specific cellular responses mediated by biomolecular recognition, which can be manipulated by altering design parameters of the material. Marine derived polysaccharide such as alginate, chitosan and hyaluronic acid has demonstrated high biocompatibility, biodegradability, non-toxicity and low chronic inflammatory response. Therefore, these materials can applicable tissue engineering application. Among them, alginate is a well-known biomaterial obtained from brown macroalgae and is widely used in biomedical, drug delivery and tissue engineering application. Moreover, since marine organism derived materials have not yet been reported with bovine spongiform encephalopathy, transmissible spongiform encephalopathy and foot and mouth disease compared to land animals. In light of the various proven, this review focuses on tissue regenerative properties of natural polysaccharides derived from marine organisms for cartilage tissue regeneration application.

메시징 서버를 이용한 콤포넌트 기반의 문서관리 시스템 설계

허성영(Sungyoung Hur),나연묵(Yunmook Nah) 한국정보과학회 2000 한국정보과학회 학술발표논문집 Vol.27 No.2Ⅰ

본 논문에서는 메시징 서버를 이용한 문서 관리 시스템을 제안한다. 본 문서관리 시스템은 각 구성 부분들을 콤포넌트 단위로설계하고, 메시징 서버를 기본 엔진으로 사용한다. 엔진으로 메시징 서버를 사용하면 각각의 문서를 하나의 객체로 처리할 수 있게 되어 문서 객체들에 대한 직접적인 관리가 가능해지므로 프로젝트 관리, 문서 권한 관리, 새로운 문서 유형의 추가 수정등이 용이하고, 문서들을 계층적 구조로 저장 검색하기에 용이하다. 이 시스템은 워크플로우와 같은 메시징 기반의 시스템과의 연동에 쉽게 적용될 수 있다.

알코올 사용장애와 알츠하이머 병 환자의 인지기능 평가 차이에 관한 비교 연구

조태형,허성영,정지운 한국중독정신의학회 2023 중독정신의학 Vol.27 No.2

Objectives: There are a few studies on how much and which cognitive function is specifically damaged by chronic alcohol drinking. By comparing neurocognitive function test results between patients with alcohol use disorder and those with Alzheimer’s disease, we aimed to find out which neurocognitive function is damaged by chronic alcohol drinking. In addition, we investigated whether the structural changes in the brain were related to cognitive function decline. Methods: Korean Wechsler Adult Intelligence Scale-IV and Seoul Neuropsychological Screening Battery-II were performed among patients who were diagnosed with Alzheimer’s disease and those who were diagnosed with alcohol use disorder at the department of psychiatry in Pusan National University Yangsan Hospital from November 2008 to May 2022. Among the neurocognitive domains, intelligence quotient, memory quotient, executive intelligence quotient, auditory attention and visual attention were compared. Among those who underwent cognitive function tests, the reading results for brain CT or MRI were compared. Results: There was no significant difference between the two groups in visual attention (p=0.219). Meanwhile, structural changes in the brain were less in the alcohol use disorder group compared to the Alzheimer’s disease group. Conclusion: Chronic drinking, impaired especially, visual attention to the level of Alzheimer’s disease at a relatively early age. Additional research on cognitive function impairment in alcohol use disorders is needed, referring to the results of this study.

Antiviral effect of fucoxanthin obtained from Sargassum siliquastrum (Fucales, Phaeophyceae) against severe acute respiratory syndrome coronavirus 2

강나래,허성영,Eun-A Kim,차선희,BoMi Ryu,허수진 한국조류학회I 2023 ALGAE Vol.38 No.4

Human coronavirus diseases, particularly severe acute respiratory syndrome coronavirus 2, still remain a persistent public health issue, and many recent studies are focusing on the quest for new leads against coronaviruses. To contribute to this growing pool of knowledge and explore the available marine natural products against coronaviruses, this study investigated the antiviral effects of fucoxanthin isolated from Sargassum siliquastrum—a brown alga found on Jeju Island, South Korea. The antiviral effects of fucoxanthin were confirmed in severe acute respiratory syndrome coronavirus 2-infected Vero cells, and its structural characteristics were verified in silico using molecular docking and molecular dynamic simulations and in vitro colorimetric method. Fucoxanthin inhibited the infection in a concentration-dependent manner, without showing cytotoxicity. Molecular docking simulations revealed that fucoxanthin binds to the angiotensinconverting enzyme 2-spike protein (binding energy -318.306 kcal mol-1) and main protease (binding energy -205.118 kcal mol-1). Moreover, molecular dynamic simulations showed that fucoxanthin remains docked to angiotensin-converting enzyme 2-spike protein for 20 ns, whereas it breaks away from main protease after 3 ns. Also, the in silico prediction of the fucoxanthin was verified through the in vitro colorimetric method by inhibiting the binding between angiotensinconverting enzyme 2 and spike protein in a concentration-dependent manner. These results indicate that fucoxanthin exhibits antiviral effects against severe acute respiratory syndrome coronavirus 2 by blocking the entry of the virus. Therefore, fucoxanthin from S. siliquastrum can be a potential candidate for treating coronavirus infection.

보티옥세틴이 C57BL/6형 생쥐의 알코올 섭취량에 미치는 영향

최세라,정지운,허성영,주병욱,김현경,김성곤 대한생물치료정신의학회 2023 생물치료정신의학 Vol.29 No.3

Objectives: The effectiveness of drugs currently used in medication, which is important in the treatment of alcohol use disorders, is limited. Recently, ondansetron which acts as 5-HT3 receptor antagonist, has been studied and proved possibility as new medication for alcohol use disorder. Meanwhile, there are studies supporting that 5-HT1A receptors are related to addictive behavior. Considering those studies, we expect that vortioxetine, which acts as both 5-HT3 receptor antagonist and 5-HT1A receptor agonist, may be effective in treatment of alcohol use disorder. The purpose of this study is to examine the effect of vortioxetine on alcohol intake of C57BL/6 mice. Methods: In this study C57BL/6 mice were randomly assigned to normal saline group, vortioxetine 10 mg/kg group and vortioxetine 1 mg/kg group. To study effect of vortioxetine on alcohol, water, food intake and body weight of mice, we administered each medication for 14 days. Results: The overall alcohol intake was different between the three groups (pGroup=0.021), and alcohol intake in vortioxetine 10 mg/kg group was significantly lower than one in placebo group. Change across time points (pTime<0.001) and the interaction between group and time (pGroup×Time=0.016) were also significant. However, there were no significant differences between the three groups in water, food intake and body weight. Conclusions: These results indicated that administration of high dose vortioxetine reduced alcohol intake of mice. Therefore, it is necessary to conduct clinical studies to examine the effectiveness of vortioxetine as a new treatment for alcohol use disorder.

바이오피드백을 이용하여 측정한 알코올 사용장애 환자의 정신생리적 특징

이신화,정지운,허성영 한국중독정신의학회 2023 중독정신의학 Vol.27 No.2

Objectives: Previously published studies have shown that hypothalamic-pituitary-adrenal axis axis dysfunction and autonomic arousal are associated with alcohol use disorder. The purpose of this study was to measure the psychophysiological responses in patients with alcohol use disorder using a biofeedback system and compare them with those of healthy subjects. Methods: Fifty-two patients with alcohol use disorder and 149 healthy controls participated in this study. Heart rate (HR), electromyography (EMG), skin conductance (SC), skin temperature, and respiratory rate (RR) were recorded using a biofeedback system during five phases of a stress reactivity test, and average values were calculated. The difference in values between the two groups in each corresponding phase was analyzed with the independent t-test, and the change in values across phases of the stress reactivity test was analyzed with the paired t-test. Results: Compared to the healthy controls, patients with alcohol use disorder had a higher HR and RR in three phases. Change in EMG and SC was higher in patients with alcohol use disorder than the healthy controls from baseline to the stress phase 1. Conclusion: The psychophysiological profiles of patients with alcohol use disorder in a stress reactivity test were different from those of healthy controls. These results suggest that the sympathetic nervous system is more highly activated in patients with alcohol use disorder.

알코올 사용 장애 환자들의 가족력 강도와 음주 특성

민은정,김성곤,허성영,김현경 알코올과 건강행동학회 2022 알코올과 건강행동연구 Vol.23 No.1

Background: It has been shown that alcohol use disorders are common within a particular family. The risk of alcohol use disorders is known to be three to five times higher for a parent than non-parent alcohol. For this reason, many studies to date have examined the family history of alcohol use problems among adolescents or early adults with drinking problems. However, most studies examined only the existence of alcohol family history, and there were no reports of familial density among alcoholics patients. Objective: The purpose of this study was to investigate the familial density of people with alcohol abuse problems and to investigate the differences in the characteristics of alcohol abuse problems according to their familial density. Method: At four community centers in Busan and Gyeongsangnam-do, familial density was investigated for clients using the service. There were 141 subjects in the study, 113 men and 28 women. Result: Of the people with alcohol use problems, familial density had four groups (family history negative, grandparents or siblings only, parent(s) only, parent(s) and grandparents or siblings, parent(s) and grandparents and siblings,). Familial density was significantly associated with frequency of violence, drinking related, AUDIT-K score. Conclusion: There is a difference in familial density among people with alcohol use problems. However, systematic comparison of variety of measures of family history is lacking. and further study is needed. This study investigated familial density in patients with alcohol use disorder. These results will be the evidence for another perspective on the genetic effects and its risks of alcohol use disorder patients.