나노기술과 해양용 센서 개발에 관한 연구

한송희,조병기,Han, Song-Hee,Cho, Beong-Ki 해양환경안전학회 2008 해양환경안전학회지 Vol.14 No.1

It is generally recognized that monitering of bio-molecules, which are related to the ocean environment, becomes more important. So far, for the detection of the bio-molecules, ocean samples were brought to laboratory to be analyzed using a complicate and expensive measuring system The "ship and dip" method takes a relatively long time to complete a analysis cycle and causes significant errors due to the time difference between the analysis processes. In order to overcome the drawbacks, developments of sensors for the detection of bio-molecules were suggested using nano-technology, such as nano-spintronic device, carbon nano tube device, and nano-semiconductors. The pros and cons of the technology were examined and reinvestigated to overcome the technical problems in the application to real sensors.

막표면의 케이크층 세정 방법에 따른 여과 저항값 비교

한송희 ( Song Hee Han ),장인성 ( In Soung Chang ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.6

The resistance in series model has been frequently used for determination of various filtration resistance to correctly understand the membrane fouling behaviour in MBR (membrane bio-reactor) for wastewater treatment. The cake layer resistance (Rc) which is commonly determined by calculation of flux dataset that are obtained empirically before and after removing the cake layer on membrane surface. However, the calculated Rc values are very dependent on the cleaning methods adapted for removal of cake layer. This study investigated how the various cleaning options affect Rc. Seven different cleaning methods were employed: i) ultrasonication (100 W, 10 min), ii) ultrasonication (200 W, 60 min), iii) ultrasonication (400 W, 120 min), iv) water rinsing in a shaker (100 rpm, 10 min), v) water rinsing in a shaker (300 rpm, 60 min), vi) water rinsing, vii) sponge scrubbing. For the hydrophilic PES membrane, the cake layer removal efficiencies ranged from 64% to 10%, indicating that the removal of cake layer was highly dependent on the cleaning options. For the hydrophobic PVDF membrane, the cake layer removal efficiencies ranged from 79% to 97%. Consequently, a standardized method for cake layer removal to determine cake resistance (Rc) is needed for correct interpretation of the fouling phenomena.

생활계 오염원의 유기물 및 암모니아성 질소 배출특성 평가

한송희 ( Song Hee Han ),김요용 ( Yo Yong Kim ),성연국 ( Yeon Gook Sung ),박익범 ( Ik Beom Park ),조덕희 ( Deok Hee Cho ),남우경 ( Woo Kyung Nam ),김창규 ( Chang Gyu Kim ),오조교 ( Jo Kyo Oh ) 한국물환경학회 2015 한국물환경학회지 Vol.31 No.4

The purpose of this research was to suggest the water quality improvement in streams by evaluating the distribution characteristics of organics and ammonia nitrogen discharged by pollution sources from human living. The public sewage treatment plants’(PSTPs) effluents and the waters from streams in Gyeonggi-do were sampled and analyzed. Nitrogenous oxygen demand (NOD) was measured for the stream waters as well as the PSTPs effluents, and the correlations of NOD and NH3-N, NH3-N and water temperature in the PSTPs effluents were confirmed. In the case of the stream waters, the ratios of NOD to BOD and NH3-N increased in the downstream sites after discharging the PSTPs effluents. As a result of statistical analysis of NH3-N concentrations for the national water quality monitoring streams in Gyeonggi-do, NH3-N showed the non-normal distribution which were biased to the left, but showed the considerable level because of higher coefficient of variation. Therefore, it is required to establish the water quality standard for the NH3-N as a new parameter for judging the quality of the streams. In addition, inducing complete nitrification and introducing a logical standard setting system are needed to improve the water quality of streams by identifying distribution of the nitrogen components from PSTPs effluents.

LC-MS/MS를 이용한 인체 혈장에서 Ginsenoside Rb1의 분석법 검증

한송희(Song-Hee Han),김윤정(Yunjeong Kim),전지영(Ji-Young Jeon),황민호(Minho Hwang),임용진(Yong-Jin Im),이선영(Sun Young Lee),채수완(Soo-Wan Chae),김민걸(Min-Gul Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.12

LC-MS/MS를 사용함으로써 인체 혈장에서 ginsenoside Rb1의 분석법을 개발하고 검증하였다. 유속 0.9 mL/min에 이동상 0.1% formic acid가 첨가된 water와 methanol을 사용하여 기울기 용리 조건으로 설정하였으며 사용한 분석 칼럼은 C 18(4.6 mm×150 mm, particle size 5 μm)을 사용하여 분리하였다. MRM(multiple reaction monitoring) 방법의 전기 분무 이온화 이온 분석기로 모니터링 하여 분석하였다. 인체 혈장 샘플은 액체-액체 추출방법에 의해 acetone과 water가 섞인 용액으로 추출하였다. 이 분석의 검량선 범위는 10~500 ng/mL이며 상관계수는 0.9995를 나타냈다. 일내, 일간의 정밀성 농도범위는 상관계수 5.8% 그리고 정확성은 96.0~104.6%로 나타났다. 이 LC-MS/MS를 이용한 인체 혈장의 ginsenoside Rb1의 연구가 약동학 연구에 적용할 수 있을 거라 생각한다. A new liquid chromatographic tandem mass spectrometric (LC-MS/MS) assay for the quantification of ginsenoside Rb1 in human plasma was developed and validated. The separation was performed on a Agilent C18 column (4.6 mm×150 mm, particle size 5 μm) with a gradient elution of 0.1% formic acid in water and 0.1% formic acid in methanol and a flow rate of 0.9 mL/min. The analyte was determined using electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode (m/z 1131.714→365.303). Human plasma samples were extracted with acetone : water (50:50) by the liquid-liquid extraction method. The method was linear over the dynamic range of 10~500 ng/mL with a correlation coefficient of r=0.9995. The intra-and inter-day precision over the concentration range of ginsenoside Rb1 was lower than 5.8% (correlation of variance, CV), and the accuracy was between 96.0~104.6%. This LC-MS/MS assay of ginsenoside Rb1 in human plasma is applicable for quantification in a pharmacokinetic study.

네트워킹 행동 유형이 조직변화준비도에 미치는 영향: 지식공유의 매개효과

한송희(Song Hee Han),홍아정(Ah Jeong Hong),김시진(Si Jin Kim) 한국성인교육학회 2021 Andragogy Today : International Journal of Adult & Vol.24 No.4

조직변화는 글로벌 경쟁 시장에서 생존하기 위한 필수 전제 조건이다. 이 연구는 구성원들의 심리에 주목하고 변화에 대한 태도와 신념, 의도를 긍정적으로 이끌 수 있도록 하는 조직변화준비도에 주목하였으며, 네트워킹 행동을 내부와 외부로 나누어 유의미한 영향이 있는지 실증하고자 하였다. 네트워킹 행동으로 인해 지식공유 과정이 일어나는 구조를 밝히고, 지식공유의 매개효과를 검증하고자 하였다. 국내 기업 구성원 294명에게 설문조사한 결과 다음과 같은 결과를 검증하였다. 첫째, 기업 구성원의 네트워킹 행동 중 외부 네트워킹 행동만 조직변화준비도에 유의미한 영향을 미치는 것으로 나타났다. 둘째, 조직 내 구성원들의 지식공유 역시 조직변화준비도에 유의미한 영향을 미쳤다. 셋째, 구성원들의 네트워킹 행동과 조직변화준비도 간에 지식공유는 매개효과를 갖는 것으로 나타났다. 이상의 연구결과를 토대로 이 연구는 기업의 변화시도에 있어 성공여부를 기업주도가 아닌 구성원의 주도적 행동과 조직의 지식경영의 관점에서 주목하여 시사점을 제안하였다. Organizational change is a prerequisite for companies to survive in this competitive global market. The study paid attention to the psychology of the members and the readiness for change that can lead to positive attitudes and beliefs or intentions for change, and to demonstrate whether there is a significant impact by dividing networking behavior into internal and external factors that influence this. In addition, it attempted to clarify the structure in which the knowledge sharing process occurs due to networking activities, and to verify the mediating effect of knowledge sharing. First, only external behavior among networking behaviors of corporate members had significant effect on organizational change readiness. Knowledge sharing has a significant effect on change readiness. Since knowledge sharing is based on trust, social capital is formed, which is a phenomenon that occurs in an environment with low anxiety. Third, networking behavior affects readiness for change through knowledge sharing. This is because networking behavior is largely based on communication, so there is an essential process in which knowledge is exchanged with each other. Finding ot the study implicates that the success in corporate change attempts was found from the perspective of members-led rather than corporate-led, and focused on individual psychological characteristics to uncover the effects.

외래 관광객의 체험지향성이 체험활동 선호도에 미치는 영향연구

한송희(Song Hee Han),윤유식(Yoo Shik Yoon),최윤지(Yoon Ji Choi) 한국관광연구학회 2012 관광연구저널 Vol.26 No.1

This study investigated the effects of foreign tourists` experience orientation for experience activity preferences in the rural tourism destination. Based literature review, tourists` experience orientation and experience activity preferences were discussed. In order to collect the data, survey questionnaires were distributed in the popular tourists destinations such as Insadong, rural tourism experiences villages, and etc. Four different languages for targeting Chinese, Japanese, East-Asian people, and English speaking countries were used in the survey questionnaire. A total of 317 useful samples were collected and analyzed in SPSS. Five different underlying dimensions for experience orientation were identified and their effects on the experiences preferences were examined. Findings and implications were discussed in the conclusion.

농촌관광마을의 체험활동 분류 및 분석 연구

한송희(Song-Hee Han),손진관(Jin-Kwan Son),최윤지(Yoon-Ji Choi),윤유식(Yu-Shik Yoon) 한국농촌지도학회 2015 농촌지도와 개발 Vol.22 No.1

Rural tourism village experience is proceeded quantitatively without distinct characteristic. This research aimed at analyzing the experience and utilizing in the establishment of differentiation and contents development. Type of experience activity was classified as 10 types in Level 1 and 0~4 types in Level 2. As the result of analyzing 3,007 experiences in 168 villages, types of experience activity implemented per 1 village was 17.9. Among them, ecological experience type appeared to be the most, and appeared in order of food, agriculture farming experience. In respect of agriculture farming experience, ‘harvest and utilization’ was analyzed to be the highest, and regarding rural farmhouse living experience displayed ‘farmhouse living’experience the highest. Tradition courtesy experience displayed‘traditional culture’ experience the highest, and rural food experience was analyzed to implement ‘food making’ experience the most. Ecological experience mainly consisted of ‘hunting and collecting’ and ‘observation/learning’, in case of play experience,‘traditional play’experience activity was analyzed to be performed the most. Considering utilization material, it appeared in order of ‘rice’, ‘sweet potato’,‘potato’, ‘corn’, ‘chili’, ‘agricultural implement’, ‘farmhouse’, ‘animal’, ‘culture’, ‘history’, ‘rice cake’, ‘alcoholic drink’, ‘tofu’, ‘kimchi’, etc. The place of ecological experience was performed in the forest the most, and lots of experience was performed in stream, valley, and river. The researcher expects that characteristic experience activity will be developed based on this result, by avoiding doubleness of the experience activity among the regions and the villages.

외국인 농촌관광 중요도속성에 따른 농촌관광 인지도 및 경험여부, 행동의도 판별분석

한송희(Song-Hee Han),윤유식(Yoo-Shik Yoon) 한국농촌지도학회 2015 농촌지도와 개발 Vol.22 No.3

This study was to investigate if there are important variables of rural tourism destination for foreign tourists that discriminate rural tourism perception, experience, and intention to behavior. of the experience activity among the regions and the villages. From the literature review, the survey questionnaires was developed to measure important variables of rural tourism destination, rural tourism perception, experiences and intention to behavior. A total of 799 useful samples were collected and analyzed in SPSS. Factor analysis showed that there are four underlying dimensions (program, preparation to receive, service and regional products, and human service). the results of discriminate analysis showed that service and regional products are important variables in rural tourism perception and experiences. And also service and regional products and human service are important variables in intention to behavior. More detailed discussion and implications are provided in conclusion.

소규모 급수 시설의 불소 및 질산성질소 이온 제거를 위한 탄소나노튜브 전극을 활용한 전기흡착 연구

한송희(Han, Song-Hee),장인성(Chang, In-Soung),채기웅(Chae, Ki-Woong),정선용(Chung, Son-Young),이철구(Lee, Chul-Ku) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.6

본 연구에서는 소규모 수도시설에서 수질기준을 자주 초과하는 항목으로 지적되고 있는 불소와 질산성질소 를 효과적으로 제거하기 위하여, 탄소나노튜브를 이용한 전극을 제작하여 전기흡착 공정에 적용함으로써 두 종류의 무기이온 제거 가능성을 평가하였다. 탄소나노튜브를 활용한 전극을 제작하기 위해 코팅 (coating)법과 소결(sintering) 법을 이용하여 전극을 제작하였다. 코팅전극은 바인더의 종류, 소결전극은 소결온도를 변화시켜 각각의 전극을 제작 하였다. 제작된 전극을 이용하여 전류밀도 및 반응시간 등을 변화시켜가며 전기흡착 실험을 진행한 결과 유기바인더 를 이용해 제작된 전극의 이온제거율은 불소 46%, 질산성질소 99.9%로 무기바인더를 이용하여 제작된 전극보다 제거 효율이 우수하였다. 또한 소결하여 제작된 탄소나노튜브 중 1000℃ 고온에서 제작한 전극은 불소 77%, 질산성질소 87%의 제거율을 보여 850℃ 저온에서 제작한 전극보다 더 높은 제거효율을 보임을 확인하였다. 적당한 전류밀도 및 반응시간 하에서 모두 먹는 물 수질 기준에 적합한 농도로 처리될 수 있음을 보임으로써, 탄소나노튜브를 활용하여 제작한 전극을 채택한 전기흡착 공정이 소규모 수도시설에 적합한 공정으로 평가받을 수 있을 것으로 판단된다. Water qualities in the decentralized water treatment plants do not frequently satisfy the water standard limit, in particular, fluoride and nitrate are notorious for the poor removal. In this study, an electro-adsorption equipped with carbon nonotube (CNT) electrodes were carried out to effectively remove the nitrate and fluoride in the decentralized water treatment plants. Two types of CNT electrodes, coating and sintering electrodes were applied. Coating electrodes were made based on different kinds of binder and sintering electrodes were made based on different sintering temperature. Removal of fluoride and nitrate when the coated electrodes with organic binder were used for electro-adsorption were 46 and 99.9% respectively, which were better performances than the coated electrodes with inorganic binder were used. On the other hand, removal of fluoride and nitrate when the electrodes sintered at higher temperature (1,000℃) were 77 and 87% respectively, which were better performances than the electrodes sintered at lower temperature (850℃). As a consequences, the electro-adsorption equipped with a CNT electrodes could be an potential alternative process for the removal of fluoride and nitrate in a decentralized water treatment plants if proper current density and contact time were applied.

백수오와 한속단 추출물의 비에스트로젠 효과에 관한 연구

한송희(Song-Hee Han),이태희(Tae-Hee Lee),장자영(Ja-Young Jang),송현경(Hyun-Kyung Song),홍상근(Sang-Keun Hong),김유리(Yu-Ri Kim),한범석(Beom-Seok Han) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.5

저성장 어린이들을 대상으로 하는 성장호르몬 치료는 다양한 부작용과 투여과정의 스트레스로 인한 환자 순응도 감소, 고가의 치료 비용 등의 단점을 가지고 있다. 이러한 이유로 부작용 발생의 위험성이 작고 낮은 비용으로 어린이의 성장에 도움을 줄 수 있는 천연물 유래 기능성 소재 개발에 대한 관심이 증가하고 있다. 그러나 성적으로 미성숙 상태인 어린이를 대상으로 한다는 점, 에스트로겐에 의한 뼈 성장과 성조숙증 발병기전 연관성, 천연물에 존재하는 다양한 식물성 에스트로겐의 영향으로 안전성에 대한 우려가 존재하는 것이 사실이다. 따라서 본 연구에서는 앞선 연구를 통하여 뼈 성장 촉진 효과가 확인된 시험물질의 안전성을 확인하기 위하여 난소를 절제한 실험동물에 시험물질을 4주간 투여한 후 시험물질이 식물성 에스트로겐으로 작용하여 성조숙증과 성장호르몬 이상을 일으킬 가능성을 확인하고자 하였다. 실험 결과 난소 절제로 인한 체중증가, 혈중 지질 농도의 증가, 자궁 벽 두께의 감소 등이 나타남을 확인하였으며 시험물질의 4주 투여가 이러한 변화에 영향을 끼치지 않음을 확인할 수 있었다. 또한 혈중 에스트로겐 농도, 혈중 성장호르몬 농도를 분석한 결과 시험물질 투여가 아무런 영향을 주지 않음을 확인하였다. 이러한 결과는 백수오와 한속단 동량 혼합물이 생체 내에서 식물성 에스트로겐으로 작용하지 않는다는 것을 의미하며 반복투여에 의한 성장호르몬 이상을 일으키지 않는 것으로 판단된다. Cynanchum wilfordii and Phlomis umbrosa Turcz. are known to contain isoflavone, a representative phytoestrogen. This study was performed to determine whether the extract mixture of C. wilfordii and P. umbrosa Turcz. would induce an estrogenic effect in ovariectomized rats. The extracts were administered to the ovariectomized rats at 30, 60, 120 mg/kg per day for 4 weeks, respectively. They showed no estrogenic effect, which was indicated by the decrease in uterus wall thickness as well as the increase in body weight and the level of cholesterol and triglyceride. The extracts also had no effect on the concentrations of estrogen and growth hormone in the serum. However, the increase in alkaline phosphatase activity, which leads to protection against the bone loss caused by ovariectomy, was noted on administration of the extract. Therefore, it seemed that the extracts of C. wilfordii and P. umbrosa Turcz. had no estrogenic effect in rats.