다낭난소증후군으로 인한 사회경제적 비용에 관한 연구

김한나 ( Han Na Kim ),정경아 ( Kyung Ah Jeong ),정혜원 ( Hye Won Chung ),배근량 ( Geun Ryang Bae ),한복기 ( Bok Ghee Han ),김형래 ( Hyung Lae Kim ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.12

Objective: The objective of the study was to estimate socioeconomic burden of polycystic ovary syndrome (PCOS) during the reproductive life span using current definitions and prevalence or incidence data. Methods: Questionnaires were given to 8,588 reproductive women reviewed at Ewha Womans University Mokdong hospital. The PCOS affected approximately 10.4% of reproductive-aged women (11 million women in Korea, prevalence rate according to 1990 National Institutes of Health PCOS diagnosis criteria). We tied general societal cost data for the different health consequences to reproductive-age PCOS costs, using prevalence data. Results: We estimated the mean annual cost of the initial evaluation to be ￦76 hundred million, that of hormonally treating menstrual dysfunction, providing infertility care, diagnosis/treatment of endometrial hyperplasia, GDM, type 2 DM, and hypertension to be ￦280 billion. The total annual socioeconomic cost of evaluating and providing care to reproductive-aged PCOS women in Korea is ￦350 billion. Conclusion: Because the cost of the diagnostic evaluation accounted for a relatively minor part of the total socioeconomic costs, more widespread screening for PCOS appears be a cost-effective strategy, leading to earlier diagnosis and intervention and possibly the amelioration and prevention of serious sequelae.

2차원 바코드와 UCC/EAN-128을 이용한 생물자원 자동인식시스템

주민석,류근호,김준우,김흥태,한복기,Chu, Min-Seok,Ryu, Keun-Ho,Kim, Jun-Woo,Kim, Hung-Tae,Han, Bok-Ghee 한국정보처리학회 2008 정보처리학회논문지D Vol.15 No.6

컴퓨팅 환경이 발전함에 따라 다양한 물리적 객체와 디지털 정보를 연동하는 자동인식 연구가 활발히 진행 중이다. 이러한 자동인식시스템은 다양한 산업분야에서 활용되고 있음에도 불구하고 보건의료와 관련한 자동인식 기술의 접목은 아직까지 다른 산업기술 전반에 미치지 못하고 있는 실정이다. 이에 따라 의료장비, 혈액, 인체조직 등 보건의료 용품의 자동인식에 관한 여러 연구가 진행 중이다. 이 논문은 인간 유전체 연구의 필수 연구재료인 생물자원을 대상으로 자동인식 기술의 적용 방안을 제안한다. 먼저, 자동인식기술 도입을 위해 사용 환경상의 고려사항을 정의하고, 조사과정 또는 실험을 통하여 적합한 형태의 태그 인터페이스로서 바코드를 선택하였다. 바코드 심볼로지는 2차원 바코드 심볼로지인 Data Matrix를 사용하고, 데이터 스키마는 국제적 범용성 추구를 위하여 UCC/EAN-128 기반으로 설계하였다. 제안된 기술들이 실제 환경에 적용되는지를 보이기 위한 어플리케이션을 개발하고, 이에 대한 실험 및 평가를 다음의 방법으로 수행하였다. 생물자원이 실제 보존되는 영하 $196^{\circ}C$, 영하 $75^{\circ}C$의 초저온 보존환경에서 바코드 인식실험을 한 결과 1.6초 내외의 평균 인식시간을 보이며, 데이터 스키마는 생물자원 활용 분야의 요구사항을 만족하는 것으로 평가되었다. 따라서 제안한 방법으로 생물자원의 정보처리 과정에서 정확성과 데이터 입력의 신속성이 제공될 수 있다. As rapid development of computing environment, field of automatic identification research which interoperates with various physical objects and digital information is making active progress. Although the automatic identification system is widely used in various industries, application of automatic identification system in the field of medical health doesn't reach other industry. Therefore research in medical health supplies such as medical equipment, blood, human tissues and etc is on progress. This paper suggests the application of automatic identification technology for biological resources which is core research material in human genome research. First of all, user environment requirements for the introduction of automatic identification technology are defined and through the experiments and research, barcode is selected as a suitable tag interface. Data Matrix which is 2D barcode symbology is chosen and data schema is designed based on UCC/EAN-128 for international defecto standard. To showapplicability of proposed method when applied to actual environment, we developed, tested and evaluated application as following methods. Experiments of barcode read time at 196 and 75 below zero which is actual temperature where biological resources are preserved resulted read speed of average of 1.6 second and the data schema satisfies requirements for the biological resources application. Therefore suggested method can provide data reliability as well as rapid input of data in biological resources information processing.

열량 및 열량영양소 섭취량과 관련된 유전자 변이에 대한 전장유전체 연관성 분석연구

백인경(Baik Inkyung),안윤진(Ahn Younjhin),이승구(Lee Seung Ku),김소리울(Kim Soriwul),한복기(Han Bok-Ghee),신철(Shin Chol) 韓國營養學會 2010 Journal of Nutrition and Health Vol.43 No.4

There has been no genome-wide association study (GWAS) for macronutrient intake as a quantitative trait. To explore genetic loci associated with total calorie and macronutrient intake, genome-wide association data of autosomal single nucleotide polymorphisms (SNPs) from Korean adults were analyzed. We conducted a GWAS in 3,690 men and women aged 40 to 60 years from an urban population-based cohort. At the baseline examination (June 18, 2001 through January 29, 2003), DNA samples of the study subjects were collected and analyzed for genotyping. The information of average daily consumption of total calorie, carbohydrate, protein, and fat was obtained from a semi-quantitative food frequency questionnaire and transformed by natural logarithm for analyses after adjustment of calorie intake. Using multivariate linear regression analysis adjusted for age, sex, and height, we tested for 352,021 SNPs and found weak associations, which do not reach genome-wide association significance, with calorie and macronutrient intake. However, a number of SNPs were found to have potential associations with macronutrient intake; in particular, signals in SORBS1 and those in PRKCB1 were likely associated with carbohydrate and fat intake, respectively. We observed an inverse association between the minor allele of the SNPs in these genes and the amount of consumption of carbohydrate or fat. Our GWAS identified loci and minor alleles weakly associated with macronutrient intake. Because SORBS1 and PRKCB1 are reportedly associated with the metabolism of glucose and lipid as well as with obesity-related diseases, further investigations on biological and functional roles of polymorphism of these genes in the relation to macronutrient intake are warranted.

인슐린비의존성 당뇨병 동물모델에서 미토콘드리아 DNA 보제수의 감소

김영미,송지현,이홍규,박경수,임선희,한복기,Suzuki, S 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.2

Background: Although genetic disorder in diabetes mellitus (DM) is not well understood, it has been suggested that the maternally inherited mitochondrial DNA which does not follow the Mendel's laws is a genetic factor for DM. It was reported that the mitochondrial DNA contents in DM patients were decreased compared to the normal control. Similar decrease in mitochondria) DNA content before DM development was tested in animal models. Method: The mitochondria) DNA (mtDNA) content in various tissues obtained from two types of non-insulin dependent diabetic rats, Goto-Kakizaki (GK) and Otsuka Long-Evans Tokushima Fatty (OLETF) rats at different ages were quantified. We also determined the quantity of hepatic COX subunit III (COX III) mRNA, and the enzyme activities of succinate dehydrogenase (SDH) and cytochrome c oxidase (COX) in mitochondria isolated from liver and skeletal muscle were measured. Results: At 6 weeks, mfiDNA content of GK rat liver was 20% decreased compared to the Wistar control, The mtDNA content of Wistar rat liver was decreased to aging from 6 weeks to 24 weeks while mtDNA in GK rat liver remains relatively constant. In case of skeletal muscle, however, mtDNA contents in GK rats were 50% decreased compared to the control at 12 and 24 week old. Similarly, OLETF and LETO control rats showed the age-dependent decrease of mtDNA content in liver and pancreas. Especially the mtDNA contents in OLETF rat tissues were reduced at the younger age than the LETO control content. That is, at 6 weeks old mtDNA contents in OLETF rat pancreas and liver were only 50% of the control. The level of mitochondrial coded hepatic COX subunit III mRNA tends to decrease with age. Despite the decrease of mtDNA content, hepatic COX III mRNA level and COX activities and SDH activities were not altered significantly, implying that the change of mtDNA contents did not damage the mitochondria) gene transcription and mitochondria) function dramatically. Conclusions: This results suggest that mtDNA contents in pancreas and liver decrease age-depehdently but it occurs at younger age in NIDDM. The decrease of mtDNA content at young age may be a cause of NIDDM.