Lipid rafts are important for the association of RANK and TRAF6

하현일,HanBokKwak,SooWoongLee,Hong-HeeKim,이장희 생화학분자생물학회 2003 Experimental and molecular medicine Vol.35 No.4

Rafts, cholesterol- and sphingolipid-rich membrane microdomains, have been shown to play an im-portant role in immune cell activation. More re-cently rafts were implicated in the signal trans-receptor (TNFR) family. In this study, we provide evidences that the raft microdomain has a crucial role in RANK (receptor activator of NF-κB) signaling. We found that the majority of the ectopically expressed RANK and substantial portion of endogenous TRAF2 and TRAF6 were detected in the low-den-sity raft fractions. In adition, TRAF6 association with rafts was increased by RANKL stimulation. The disruption of rafts blocked the TRAF6 trans-teraction betwen RANK and TRAF6. Our obser-vations demonstrate that proper RANK signaling requires the function of raft membrane microdo-mains.

Modulation of the caveolin-3 and Akt status in caveolae by insulin resistance in H9c2 cardiomyoblasts

하현일,박윤배 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.3

We investigated glucose uptake and the trans-location of Akt and caveolin-3 in response to insulin in H9c2 cardiomyoblasts exposed to an experimental insulin resistance condition of 100 nM insulin in a 25 mM glucose containing media for 24 h. The cels under the insulin resistance condition exhibited a decrease in insulin-sti-mulated 2-deoxy[3H]glucose uptake as compared to control cels grown in 5 mM glucose media. translocation to membranes, we observed a sig-nificant decrease in insulin-stimulated membrane asociation of phosphorylated Akt with a con-sequent increase of the cytosolic pol. Actin remodeling in response to insulin was also greatly retarded in the cells. When translocation of Akt and caveolin-3 to caveolae was examined, the insulin resistance condition attenuated locali-zation of Akt and caveolin-3 to caveolae from cytosol. As a result, insulin-stimulated Akt activa-tion in caveolae was significantly decreased. crease of glucose uptake into the cells is related to their reduced levels of caveolin-3, Akt and phosphorylated Akt in caveolae. We conclude that the insulin resistance condition induced the retardation of their translocation to caveolae and in turn caused an attenuation in insulin signaling, namely activation of Akt in caveolae for glucose uptake into H9c2 cardiomyoblasts.

지역경제개발 네트워크가 지역경제개발 입지인센티브에 미치는 영향

하현상(河顯相) 한국정책과학학회 2012 한국정책과학학회보 Vol.16 No.3

이 연구는 미국 지방정부의 입지인센티브 제공에 대한 지역경제개발 네트워크의 영향을 분석함으로서 한국에 대한 시사점을 도출하고자 한다. 경제개발을 위한 입지인센티브 제공은 네트워크에 기반한 정치적 협상에 의해서 결정되는 경향이 있다. 따라서 지역경제개발 네트워크가 입지인센티브 제공에 어떻게 영향을 미치는지를 확인하는 것은 중요한 의의를 갖는다. 그러나 기존연구는 경제개발을 위한 입지인센티브 제공에 대한 네트워크의 영향을 간과하는 경향이 있었다. 따라서 이 연구는 네 가지 유형의 지역경제개발 네트워크 (공공조직, 민간개발조직, 공공-민간개발조직, 지역주민조직 네트워크)가 지방정부의 다양한 입지인센티브 제공에 어떻게 영향을 미치는지를 확인하였다. 분석결과는 민간개발조직 네트워크와 공공-민간개발조직 네트워크가 지방정부의 다양한 유형의 입지인센티브를 제공하도록 촉진시키는데 유의미한 영향을 미치는 것으로 분석되었다. 특히, 공공-민간개발조직 네트워크가 민간개발조직 네트워크보다 입지인센티브 제공에 더 큰 영향을 미치는 것으로 나타났다. 오늘날 한국 지방자치단체들도 선호시설과 혐오시설 입지를 둘러싼 활발한 네트워크 활동을 하고 있는 점을 감안할때 이 연구의 결과는 중요한 시사점을 제공한다. 우리나라 지방자치단체의 정책결정자들은 효율적 지역경제개발을 위해서 민간개발조직이나 공공-민간개발조직 네트워크와 같은 영향력 있는 조직들의 네트워크 활동에 더 많은 관심을 가지고 보다 체계적으로 면밀히 관리할 필요가 있을 것이다. This study investigates the influence of local economic development networks on location incentive offer of American local governments. Location incentive offer closely ties to political bargaining based on local economic development networks. However, previous studies are likely to overlook the influence of networks on location incentive offer for local economic development. Therefore, this study explored how local economic development networks impact several kinds of location incentive offers: public organizational, private development organizational, public-private development organizational, neighborhood organizational networks. The findings suggest that private development organizational networks and public-private development organizational networks have significant impact on the location incentive offers. Especially, public-private development organizational networks have stronger influence on location incentive offers than private development organizational networks. This results provide critical implication in that policy decision makers of Korean local governments need to systematically manage influential networks such as private development organizational and public-private development organizational networks.

Water extract of Uncaria sinensis suppresses RANKL-induced bone loss by attenuating osteoclast differentiation and bone resorption

하현일,심기석,마진열 한국한의학연구원 2017 Integrative Medicine Research Vol.6 No.4

Background: The hooks and stems of Uncaria sinensis have been used to mitigate cardiovascular and central nervous system disorders in Asia traditional medicine. Regulation of osteoclast differentiation and activity is a major target for preventing and treating pathological bone diseases. Methods: Tartrate-resistant acid phosphatase (TRAP) activity and the number of TRAP-stained multinucleated cells were used to examine receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. The activation of RANKL-induced signaling pathways and the expression of transcription factors were investigated by western blot analysis and quantitative real-time polymerase chain reaction. The bone resorption activity of osteoclast was studied using a plate coated with hydroxyl-apatite. Trabecular bone destruction was investigated using a RANKL-induced trabecular bone loss mouse model. Results: We found that water extract of the hooks and stems of U. sinensis (WEUS) inhibits RANKL-induced differentiation of murine bone marrow macrophages and RAW264.7 cells into osteoclasts. WEUS inhibited the activation of NF-κB and the expression of nuclear factor of activated T-cells, cytoplasmic 1. In addition, WEUS suppressed the bone resorbing activity of mature osteoclasts without affecting their survival. Furthermore, oral administration of WEUS suppressed RANKL-induced bone loss with a significant amelioration of trabecular bone micro-structures. WEUS also reduced RANKL-induced increase in serum TRAP5b activity and C-terminal cross-linked telopeptide of type I collagen levels. Conclusion: The present study demonstrates that WEUS has a pharmacological activity that inhibits osteoclast-mediated bone destruction by suppressing osteoclast differentiation and function. These results suggest that U. sinensis could be a promising herbal candidate for preventing and treating bone diseases such as osteoporosis.

축계 진동 저감을 위한 수직형 안내 베어링의 최적 설계

하현천(Hyun Cheon Ha),박철현(Chul Hyun Park),김형자(Hyung-Ja Kim) 한국동력기계공학회 2000 한국동력기계공학회 학술대회 논문집 Vol.- No.-

양수 발전소 펌프-터빈의 구조물 진동 저감 대책

하현천(Hyun Cheon Ha),류석주(Seok Ju Ryu),박철현(Chul Hyun Park) 한국동력기계공학회 1999 한국동력기계공학회 학술대회 논문집 Vol.- No.-

賀序

河炫綱 역사실학회 2000 역사와실학 Vol.17·18 No.-

賀序

河炫綱 역사실학회 2000 역사와실학 Vol.15·16 No.-

긴급중재인 제도관련 중국 중재기관의 규정 및 태도

하현수 한국중재학회 2016 중재연구 Vol.26 No.4

중국은 국제상사중재제도의 변화에 대응하기 위하여, 상해국제경제무역중재위원회(SHIAC)가 중국(상해)자유무역시험지구 중재규칙을 2014년 5월 1일 시행하면서 중국 최초로 긴급중재인 제도를 규정하였다. 그리고 중국국제경제무역중재위원회(CIETAC)도 2015년 1월 1일자로 개정한 중재규칙에서 긴급중재인 제도를 규정하였다. 그러나 중국은 민사소송법 및 중재법에서 법원만이 보전처분을 내릴 수 있도록 하고 있는 상황에서, SHIAC와 CIETAC이 긴급중재인의 임시적 처분 결정권을 인정한다는 것은 상당한 모순을 야기하고 있다. 본 논문은 SHIAC와 CIETAC의 중재규칙에 규정된 긴급중재인 제도와 관련한 주요 내용을 긴급중재인 제도를 운영하고 있는 대표적 중재기관들의 중재규칙과 상호 비교분석하였다. 또한 중국 법률에서 규정하고 있는 보전처분관련 규정과 SHIAC와 CIETAC의 관련 규정을 상호 비교하여 적용상의 특징 및 문제점 등을 파악하였다. In order to cope with the changes of International Commercial Arbitration, the Shanghai International Economic and Trade Arbitration Commission (SHIAC) regulated an Emergency Arbitrator for the first time, implementing the arbitration rules in China (Shanghai) Pilot Free Trade Zone on May 1, 2014. Moreover, the China International Economic and Trade Arbitration Commission (CIETAC) also regulated the Emergency Arbitrator in the revised arbitration rules on January 1, 2015. However, it caused considerable contradiction that SHIAC and CIETAC admitted an interim measure decision by the Emergency Arbitrator under the circumstance that the Chinese court can impose a preservative measure in the Civil Procedure Code (CPC) and Arbiration Act. This study attempted to compare the main contents of an Emergency Arbitrator regulated in the arbitration rules of SHIAC and CIETAC with arbitration rules of representative arbitral institutions which operate an Emergency Arbitrator. In addition, this study verified the application features and problems through comparing the rule of SHIAC and CIETAC with the rule related to the preservative measure in Chinese law.

SELF-ORGANIZING FEDERALISM: COLLABORATIVE MECHANISMS TO MITIGATE INSTITUTIONAL COLLECTIVE ACTION DILEMMAS

하현상 한국행정학회 2011 International Review of Public Administration Vol.16 No.1