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포도과피에 특이적으로 발현하는 stilbene synthase cDNA의 분리 및 과실발달 단계별 발현양상 분석
피재호,이호림 단국대학교 1999 論文集 Vol.34 No.-
Resveratrol (stilbene), a phytoalexin, is produced by stilbene synthase from malonyl-CoA and p-coumaroyl-CoA as substrates and its biosynthesis is induced by environmental stresses such as fungal elicitor and UV. In order to elucidate its role as an anti-stress compound, stilbene synthase (stsy) cDNAs were isolated from Jyoho grape skin, which has been known to contain high quantity of resveratrol, and their expressions were analyzed during the berry development. RT-PCR analysis showed that stilbene synthase gene was expressed in higher level in leaves and flowers than in berries, and its expression in berries was specific to skin. The expression was first detected 10 weeks postflowering in berry skin and increased as berries grew and ripened. Interestingly, another homolog was amplified from the RT-PCR analysis. Its expression was observed at the early developmental stage and reached at the maximum level 8 weeks postflowering, but was not detected thereafter. Both of the PCR produts were subcloned and they shared 70% homology in the nucleotide sequence. These results suggest that there are two or more stilbene synthase genes differentially regulated during the berry develepment.
돌발 잔디병 발생원인과 잔디혹응애(Aceria zoysiae)의 유연관계 분석
정덕웅,박대섭,이석찬,피재호,이경열 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
최근 피해가 확산되고 있는 돌발성 잔디병인 잔디잎말림병의 발생실태를 조사해 본 결과 난지형 잔디로 식재된 국내 골프장의 60% 이상 발생이 확인되었다. 이 병은 잔디의 밀도저하와 황화현상을 일으켜 품질저하를 초래하는데 잔디관리에 심각한 피해를 끼치는 것으로 알려져 있다. 잔디잎말림병의 원인을 분석하기 위하여 안양, 안성, 부산, 경북, 장성에 골프장 5곳의 잔디를 채집하였고 채집된 모든 샘플에서 응애가 집단 서식하고 있는 것을 관찰했으며 형태적 종 동정 결과 잔디혹응애(Aceria zoysiae)로 판단되었다. 잔디혹응애는 체장이 180-280μm로 육안 관찰이 불가능하며 잎을 말아 군집을 형성하는 특징을 가지고 있기 때문에 조기예찰 및 진단에 어려움이 있으므로 유전자 분석을 실시하였다. 한 개체에서 DNA 추출하여 ribosomal DNA ITS2 영역의 염기서열을 분석한 결과, Aceria guerreronis와 88% 일치하였다. 또한, 건전한 잔디에 잔디혹응애를 접종하여 관찰한 결과 약 2-3주 후에 잔디잎말림 현상이 100% 관찰되었다. 잔디혹응애는 잔디잎말림병 발생의 주요 원인으로 판단이 되며 잔디혹응애 발생시기의 조기예찰을 통하여 잔디잎말림병 발생을 억제하는데 기여할 것으로 판단된다.
길준영,피재호,Kihl, Joon-Yeong,Pyee, Jae-Ho 한국생명과학회 2007 생명과학회지 Vol.17 No.8
VvMSA, a grapevine ASR which is highly inducible by sugar and abscisic acid signals was previously shown to be a transcription factor for a hexose transporter gene VvHT1. We isolated a cDNA clone, VlASR which is regulated temporally during the grape berry development by ACP RT-PCR (annealing control primer reverse transcriptase-polymerase chain reaction) and it proved identical to VvMSA. RT-PCR and real-time PCR analyses revealed that the VlASR gene was expressed in berries at fruit set and that its expression increased as berries aged but decreased at the late ripening stage. In order to understand the regulatory mechanism of the asr gene, a genomic fragment was cloned from grapevine. The genomic DNA was 1375 bp long and a sugar box (sucrose box 3 and sucrose responsive element 1) was identified in the 611 bp upstream region of the open reading frame. Analysis of the VlASR promoter::reporter gene fusion demonstrated that this promoter was expressed in transgenic Arabidopsis even without sucrose treatment. This result suggests that the ASR/VvHT1-mediated sugar/ABA signaling, previously reported in grapevine, may not function in Arabidopsis which has no ASR homologue. 포도 ASR (VvMSA) 단백질은 hexose transporter 유전자 VvHT1의 전사를 조절하는 조절 인자 중의 하나로서 sugar 및 abscisic acid (ABA) 신호에 의해 발현이 유도된다. 본 연구진은 ACP RT-PCR (annealing control primer reverse transcriptase-polymerase chain reaction) 방법을 이용하여 포도 과실발달 과정에서 조절되는 유전자 중 VvMSA와 동일한 cDNA (VlASR)를 클로닝하였다. 이 유전자는 착과 시기에 발현되기 시작하여 과실이 발달하면서 점점 증가하여 착과 후 10 주에 가장 많이 발현되며, 숙기 후반에는 도리어 발현양이 감소하였다. 포도 asr 유전자의 조절기작을 밝히기 위해, 이 유전자의 genomic clone을 분리하였다. 총 1375 bp로 이루어진 이 유전자 절편에는 open reading frame과 100 bp의 intron을 포함하고 있다. 약 600 bp 길이의 프로모터 내에는 sugar 신호전달과 연관이 있는 것으로 알려진 sugar box(sucrose box 3 +sucrose response box 1)가 있다. 프로모터 절편을 reporter 유전자와 연결하여 Arabidopsis에 도입하고 형질전환체를 분석한 결과, reporter 유전자는 sucrose 처리와 상관없이 항상 발현되었다. 이러한 결과는 포도에서 보고된 ASR/VvHT1를 매개로 하는 sugar/ABA 신호전달계가 asr 유전자가 없는 Arabidopsis에서는 작동되지 않음을 시사하고 있다.
국내분리 돼지 전염성 위장염 바이러스의 antigenic site A와 D를 포함하는 spike gene의 염기서열 분석
권혁무,피재호,성환우,Kwon, Hyuk-moo,Pi, Jae-ho,Seong, Hwan-woo 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.2
The nucleotide sequences of spike (S) glycoprotein containing antigenic sites A and D of TGEV isolated in Korea were determined and compared with published sequences for TGEVs. The TGEV 133 and DAE5 strains had 97.40% nucleotide sequence similarity. The overall nucleotide sequence similarity of the 133 and DAE5 strains compared with other TGEV strains was between 96.86% and 99.15%. The similarity of the predicted amino acid sequence of the 133 and DAE5 strains was 94.93%. The TGEV 133 and DAE5 strains had 94.93-98.61% amino acid similarity with published sequences of other TGEV strains. The sequences of amino acid codons in the antigenic sites A and D were identical among all the viruses although there were several nucleotide changes in region containing antigenic sites A and D of Korean TGEV isolates. By phylogenetic analysis of the sequences, two Korean isolates 133 and DAE5 seemed to be derived from different lineages. These studies showed that a distinct difference in genome exists among TGEV field strains isolated in Korea.
Streptomyces coelicolor A3(2)의 actinorhodin생성에 미치는 아미노산의 영향
김재헌,피재호,오충훈,최정은 단국대학교 1996 論文集 Vol.30 No.-
We investigated the fatty acid compositions before and after the actinorhodin producrion in Streptomyces coelicolor A3(2). The relatice proportion of 15 and 17 carbon branched-chain fatty acids decreased signifi-cantly during the actinorhodin production. Fatty acid compositions in the mycelia grown in the presence of L-arginine as a sole nitrogen source, which supported actinorhodin production, showed higher relative pro-portion of the i15:0, i17:0 and ai17:0 branched-chain fatty acids. Therefore, it was concluded that common precursors derived from the amino acid were used for actinorhodin and fatty acid biosynthesis.