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Oil 의 종류 및 첨가수준이 반추위 미생물에 의한 C18 - 불포화지방산의 Hydrogenation 및 반추위 박테리아의 Oleic Acid 이용에 미치는 효과
송만강,최성호 ( M . K . Song,S . H . Choi ) 한국축산학회 1998 한국축산학회지 Vol.40 No.1
An in vitro study was conducted to examine the effects of source and addition level of oil on rumen microbial degradation of oil, fermentation characteristics, biohydrogenation of C_(18)-unsaturated fatty acids(UFA) and direct incorporation of oleic acid (C_(18:1)) by rumen bacteria in vitro. Mixed fish oil of herring(C. harengus) and mackerel(S. scombrus), and flaxseed(G. usitatissimus) oil were absorbed into cellulose powder at the levels of 3, s and 7%. ^(14)C-oleic acid (μCi/10㎎) was also addeded based on C_(18:1) content of each oil to estimate direct incorporation by ruminal bacteria. Approximately 3g of cellulose powder absorbed oil in nylon bag was incubated in 300m1 solution mixture(200m1 rumen fluid + 100㎖ buffer) for 24h(39℃) anaerobically. Solution mixture without oil(blank) was also incubated. Degradations of dry matter and oil were in the range of 26.6∼31.0% and 72.9∼77.2%, respectively. However, they were not affected by the sources and addition levels of oil. Unlikely to the pH and total VFA, ammonia-N concentration in the solution mixture after 24h incubation was lowered(P$lt;.05) by the fish oil addition without difference between the oils. Higher(P$lt;.05) molar proportions of acetate with lower propionate proportions, however, were observed from the oil additions then those from the blank. The source and addition levels of oil did not influence on total viable bacterial number. The addition of fish oil increased(P$lt;.05) proportions of palmitoleic acid(C_(16:1)), C_(18:1), linolenic acid(C_(18:3)), arachidic acid(C_(20:0)), gondoic acid(C_(20:1)) and behenic acid(C_(22:1)), but decreased(P$lt;.05) linoleic acid(C_(18:2)) and lignoceric acid(C_(24:1)) in the solution mixture after 24h incubation. The addition of flaxseed oil increased(P$lt;.05) proportions of C_(18:3), C_(20:0) and C_(20:1) Long chain fatty acid proportions of solution mixture as derived from degraded oil were not influenced by the oil levels, but higher proportions of palmitic acid, C_(16:1), C_(18:1), arachidonic acid and C_(22:0) were observed from the fish oil addition while lower proportions of C_(18:2) and C_(18:3) from the flaxseed oil addition. The additions of fish oil and flaxseed oil increased C_(18:0) proportion in the solution mixture after 24h incubation by 50.60∼51.32% and 59.54∼62.33%, respectively, compared to those prior to incubation, but highly decreased proportions of C_(18)-UFA. Direct incorporations of C_(18:1), by ruminal bacteria as estimated using ^(14)C_(18:0) were 40.4∼49.1%, but they were not affected by the sources and addition levels of oil.