인간진피섬유아세포에서 Nobiletin이 Mitogen-Activated Protein Kinases를 통한 Matrix-Metalloprotease 1 발현조절에 미치는 영향

차화준,김영주 한국피부과학연구원 2014 대한피부미용학회지 Vol.12 No.6

Nobiletin is widely used for the treatment of cancer and inflammation disorder. In neurocytes andmelanocytes, nobiletin exposure induced dendrite formation. Recently, nobiletin is suggested as a newwhitening agent. However, in dermis, effects of nobiletin are not understood currently. Therefore, it isimportant to determine how nobiletin exerts its therapeutic action and its effect on the anti-wrinkle. Todetermine whether MMP1 gene expression is regulated by nobiletin, we measured MMP1 mRNA expressionin nobiletein-treated human dermal fibroblasts (HDFs). And we showed that transactivation activity of TPAresponsive element (TRE) which is a major regulation promoter element and mitogen-activated proteinkinase (MAPK) activity which main regulator of AP-1 complex. As shown results, nobiletin was decreaseexpression level of MMP1 mRNA in oxidative-stressed HDFs. In addition, nobiletin repressed MAPKphosphorylation and transcriptional activity of AP-1 complex in oxidative-stressed HDFs. Therefore, nobiletinprotects oxidative-stress through repressing MAPK phosphorylation and transcriptional activity of AP-1complex. In addition, these results suggest that nobiletin is a potential cosmetic ingredient repressed antiagingand anti-wrinkle in skin.

Image J 프로그램을 사용한 모공측정 및 노화도의 새로운 정량분석법개발과 화장품 인체적용시험에의 활용

차화준,권승빈,김지현,김수경,안인숙,안성관 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.4

Sebum is emitted by skin pores, the openings of sebaceous glands. Skin pore size increases due to excessive sebum emission and decreased skin elasticity caused by aging. These larger skin pores result in cosmetic problems. Therefore, methods to regulate skin pore size were examined. Additionally, effective methods for measurement of skin pores are also needed. In the present study, we developed a novel quantitative method for evaluation of skin pore area and degree of aging using the Image J software. First, we evaluated the pixel size of skin pores following the application of pore-tightening cream to the skin using the Cosmetic Full-Face Photography System, which is used to measure skin pore size, as well as a novel system that combines both microscopy and Image J analysis. In both the Cosmetic Full-Face Photography System and the novel system, skin pore size decreased following application of pore-tightening cream; however, the novel skin pore detection system effectively determined the decreases in skin pore size. Additionally, the degree of aging of skin pores was determined using Feret’s diameter. Thus, we suggest that this novel skin pore-detection system can be utilized as a substitute for the existing skin pore-detection method.

기술도입 점진적 자유화 - 식품제조기술 정보 도입이래 8백여건 도입 -

차화준 한국식품산업협회 1977 좋은식품 Vol.41 No.-

NF-κB 활성 조절을 통한 Lactobacillus johnsonii 파쇄액의 항염 효과

차화준 사단법인 대한화장품학회 2023 대한화장품학회지 Vol.49 No.3

본 연구에서는 김치유래의 Lactobacillus johnsonii의 anti-inflammation 효능을 확인하였다. Rat 유래대식세포인 Raw 264.7세포에 Lactobacillus johnsonii 파쇄물을 처리하여 염증유발 marker인 TNFα와 IL1β의발현량을 확인한 결과 250 μg/mL 추출물을 처리시 1 μg/mL LPS를 처리한 대조군 대비 각각 40.55%와 34.66%의TNFα와 IL1β의 발현량 감소를 확인하였다. 또한 LPS에 의한 cytokine 발현의 핵심 전사인자인 NF-κB의 전사활성을 확인한결과 1 μg/mL LPS를 처리한 대조군 대비 NF-κB의 전사활성은 40.76% 억제됨을 확인하였다. 때문에본 연구결과를 통해 Lactobacillus johnsonii 파쇄물은 LPS에 의한 cytokine의 발현을 방지 및 NF-κB전사활성조절을 통해 항염 또는 피부진정 기능성 소재로써 가능성을 확인하였다. In this study, the anti-inflammation efficacy of Lactobacillus johnsonii derived from Kimchi was investigated. Raw 264.7 cells, which are rat-derived macrophages, were treated with Lactobacillus johnsonii lysate to confirm the expression level of TNFα and IL1β, which are inflammatory markers, and when treating 250 µg/mL extract, the exp ression level of TNFα and IL1β decreased by 40.55% and 34.66% compared to the control group treated with 1 µg/mL LP, respectively. In addition, as a result of confirming the transcriptional activity of NF-κB, a key transcription factor in cytokine expression by LPS, it was confirmed that the transcriptional activity of NF-κB was 40.76% inhibited compared to the control group treated with 1 µg/mL LPS. Therefore, the results of this study confirmed that Lactobacillus johnsonii lysate is likely to be an anti-inflammatory or skin-soothing functional material by preventing the expression of cytokine by LPS and controlling NF-κB transcriptional activity.

인간진피섬유아세포에서 코코아 주요성분인 Procyanidin B1이 JNK-AP1-TRE Axis를 통한 Matrix-Metalloprotease 1 발현조절에 미치는 영향

차화준,김영주 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.6

Cocoa extracts contain various anti-oxidant which play as anti-skin aging agents. Procyanidin B1 is one of major ingredients in cocoa. However, in dermis, effects of Procyanidin B1 are not understood currently. Therefore, we investigated whether procyanidin B1 regulated extra-cellular matrix (ECM) and exerts its therapeutic action and its effect on the anti-wrinkle. We show firstly cytotoxicity of procyanidin B1. Under 100 μg/ml procyanidin B1 show no cytotoxicity in normal human dermal fibroblasts (nHDFs). In addition, we measured matrix metalloprotease 1 (MMP1) mRNA expression in procyanidin B1 exposed nHDFs. As shown results, procyanidin B1 repressed oxidative stress induced increase of MMP1 mRNA expression. And procyanidin B1 repressed activity of TPA responsive element (TRE) which is DNA binding site of AP-1 complex which consist c-Jun and c-Fos. JNK-c-Jun axis is implicated oxidative stress-mediated MMP1 mRNA regulation. In addition, JNK is activated using phosphorylation of JNK. Therefore, we shown phosphorylation in procyanidin B1-exposed nHDFs. As shown results, procyanidin B1 decreased oxidativemediated JNK phosphorylation of JNK. Overall, our results suggest procyanidin B1 is a potential cosmetic ingredient repressed antiaging and anti-wrinkle in skin using repression of oxidative-induced MMP1 expression.

B16F10 mouse melanoma 세포에서 Cnidium officinale Makino 추출물의 α-MSH에 의해 유도된 melanogenesis 억제효과

차화준 한국피부과학연구원 2018 아시안뷰티화장품학술지 Vol.16 No.1

목적: 피부의 색을 결정하는 것은 피부, 모발, 눈, 심장, 뇌 등에 널리 퍼져있는 melanin 이라는 색소이다. Melanin은 자외선을 차단하고, 활성산소를 제거하여 피부를 보호하는 역할을 한다. 그러나 비이상적인 melanin의 축적은 과색소침착증이라는 질환을 일으키고, 대표적인 예로는 기미, 주근깨 등이 있다. Cnidium officinale Makino는 한국, 일본, 중국 등의 동아시아에서 많이 사용되는생약성분으로 한국에서는 천궁으로 불린다. 본 연구에서는Cnidium officinale Makino 추출물의 melanin 생성 억제효과를 B16F10 mouse melanoma 세포에서 확인하고자 한다. 방법: Cnidium officinale Makino를 70% ethanol에 침지하여 유효성분을 추출하고, 이를 B16F10에 처리하여 대조군에 비해서 melanin생성량이 감소하는지를 확인한다. 또한 tyrosinase 활성, tyrosinase mRNA 와 단백질 발현, MITF의 전사활성을 확인하여 Cnidium officinale Makino 추출물의 효능을 평가한다. 결과: Cnidium officinale Makino 추출물은 melanin합성을 감소시키는 것으로 확인되었고, 이러한 melanin의 감소는 melanin을 합성하는 효소인 tyrosinase 의 mRNA와 단백질의 발현 감소로 인한 활성감소로 인해 일어나는 것을 확인하였다. 또한 tyrosinase 전사인자인 MITF의 전사활성을 확인한 결과 Cnidium officinale Makino 추출물에 의해서 감소함을 확인하였다. 결론: 본 연구결과를 통해서 Cnidium officinale Makino 추출물의 melanin 합성 저해능력을 확인하였고, 과색소침착증을 감소시킬 수 있는 미백원료로 가능성을 보여주었다. Purpose: Skin color is determined by melanin distributed in skin, hair, eye, heart, and brain. The melanin protects skin against ultraviolet (UV) and scavenges cellular reactive oxygen species (ROS). However, the abnormal accumulation of melanin induces hyperpigmentation diseases such as melisma, freckle etc.. Cnidium offiinale Makino commonly used to natural medicine in East Asia such as Korea, Japan and China, is called “Chun-kung” in Korea. In present study, we identified the antimelanogenic effect of Cnidium offiinale Makino in B16F10 cells. Methods: Cnidium officinale Makino extracts were prepared by leaching in 70% ethanol. Comparing with control and Cnidium officinale Makino extracts treated B16F10, we identified melanin contents measured by optical density at 450 nm, tyrosinase activity, tyrosinase mRNA and protein expression, MITF activity using MITF. Results: As shown results, we demonstrated that Cnidium officinale Makino extracts down-regulated melanin synthesis using down-regulating activity and expression of tyrosinase which is key enzyme to produce melanin. In addition, we revealed expression of tyrosinase is regulated by MITF through repressing MITF transcriptional activity. Conclusion: Cnidium officinale Makino extracts has potential to repress melanogenesis and decreased of hyperpigmetation and to use as a cosmetic ingredient.

Mistral급과 RS:X급에 대한 장비의 비교분석

차화준(Hwa-Jun Cha),강신범(Sin-Buem Kang) 한국마린엔지니어링학회 2006 한국마린엔지니어링학회 학술대회 논문집 Vol.2006 No.-

Mistral급과 RS:X급에 대한 장비비교를 통한 국내선수들의 육성 방안 및 장비의 기술적인 면을 비교분석하여 국제 공식대회(올림픽, 아시안게임 등)에서 좋은 결과를 얻고 자 하며 윈드서핑 경기정의 발전 방향을 모색하고 분석하는데 자료로 활용되고자 한다.

Tyrosinase 발현 조절을 통한 Panax vietnamensis 추출물의 Anti-pigmentation 효과

김영주,차화준 사단법인 대한화장품학회 2022 대한화장품학회지 Vol.48 No.1

In this study, the anti-pigmentation efficacy of Panax vietnamensis (P. vietnamensis), a ginseng native to Vietnam, was confirmed. Melanin synthesis was repressed by ethanolic extracts of P. vietnamensis in B16F10 cells, melanocytes originated from mouse. At 250 µg/mL ethanolic extracts of P. vietnamensis, melanin contents were repressed by 64.04% compared to the control group. In addition, ethanolic extracts of P. vietnamensis downregulated tyrosinase activity and expression to 53.34% and 59.39%, respectively. As shown our result, ethanolic extracts of P. vietnamensis blocks α -MSH-mediated melanogenesis and is valuable whitening ingredients in cosmetics. 본 연구에서는 베트남에서 자생하고 있는 인삼인 Panax vietnamensis (P. vietnamensis)의 antipigmentation 효능을 확인하였다. 마우스 유래 melanocytes인 B16F10세포에 P. vietnamensis 70% ethanol 추출물을 처리하여 melanin의 생성량을 확인한 결과 250 µg/mL 추출물을 처리시 100 ng/mL α-MSH를 처리한대조군 대비 64.04%의 melanin 생성억제를 확인하였다. 또한 melanin합성에 주요한 단백질인 tyrosinase의활성 및 발현을 100 ng/mL α-MSH를 처리한 대조군 대비 tyrosinase의 활성은 53.34%, tyrosinase의 발현은59.39%의 억제율을 보였다. 본 연구결과를 통해 P. vietnamensis 70% ethanol추출물은 α-MSH에 의한 melanin 생성을 방지하여 미백 기능성 소재로써 가치가 있는 것으로 사료된다.

인간진피섬유아세포에서 병풀(Centella asiatica )과 엽산(Follic aicd)을 주성분으로 하는 복합발효추출물과 Phytosphingosine-1-phosphate의 복합처리의 산화적 스트레스 보호 시너지효과

이강태,이광식,차화준,안성관,안규중,김수영,이건국 한국피부과학연구원 2014 대한피부미용학회지 Vol.12 No.6

The present study aims to evaluate synergetic effects of co-treatment with Centella asiatica (C. asiatica )and follic acid-ferment extracts (CFFE) and phytosphingosine-1-phoaphte (PhS1P). Cosmetic effects ofeach of CFFE and PhS1P are relatively well-known. However, synergetic effects of co-treatment with CFFEand PhS1P are not understood currently. Therefore, we investigated synergetic effects of co-treatmentwith CFFE and PhS1P and how co-treatment with CFFE and PhS1P exerts its synergetic effects. First, wedetermined that co-treatment with specific-concentration of CFFE and PhS1P increased cell viability. At sameconcentration of CFFE and PhS1P, HDFs were resisted oxidative stress-dependent cell growth arrest. Inaddition, we show that co-treatment of CFFE and PhS1P repressed c-Jun N-terminal kinase (JNK) activationand JNK-mediated oxidative stress response in HDFs. And co-treatment of CFFE and PhS1P-mediatedblocking of JNK activation induced decrease of MMP1 mRNA expression. Therefore, co-treatment of CFFEand PhS1P synergetic protects oxidative-stress through repressing JNK phosphorylation and transcriptionalactivity of AP-1 complex. And these results suggest that cosmetics contained with both CFFE and PhS1Pmay have more therapeutic effects against oxidative-stress-mediated skin aging and formation of wrinkle.

Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells

배승희,이은미,차화준,김가람,윤영민,이현진,김종란,김유정,이홍기,정회경,민유홍,안성관 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.3

Resveratrol is a plant phenolic phytoalexin that has been reported to have antitumor properties in several types of cancers. In particular, several studies have suggested that resveratrol exerts antiproliferative effects against A549 human non-small cell lung cancer cells; however, its me-chanism of action remains incompletely understood. Deregulation of microRNAs (miRNAs), a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with lung cancer. In this study, we demonstrated that resveratrol treatment altered miRNA expression in A549 cells. Using microarray analysis, we identified 71 miRNAs exhibiting greater than 2-fold expression changes in resveratrol-treated cells relative to their expression levels in untreated cells. Furthermore, we identified target genes related to apoptosis, cell cycle regulation, cell proliferation, and differentiation using a miRNA target-prediction program. In conclusion, our data demonstrate that resveratrol induces considerable changes in the miRNA expression profiles of A549 cells, suggesting a novel approach for studying the anticancer mechanisms of resveratrol.