한국인의 대장암 세포주에서 p53 돌연변이의 발견과 발현에 관한 연구

정지연,오상진,Jung, Ji-Yeon,Oh, Sang-Jin 대한면역학회 2001 Immune Network Vol.1 No.2

Background: Inactivation in p53 tumor suppressor gene through a point mutation and deletion is one of the most frequent genetic changes found in human cancer, with 50% of an incidence. This high rate of mutation mostly suggests that the gene plays a central role in the development of cancer and the mutations detected so far were found in exons 5 to 8. Mutation of p53 locus produced accumulation of abnormal p53 protein, and negative regulation of cell proliferation and transcriptional activation as a suppressor of transformation were lost. In addition, inhibition of its normal cellular function of wild-type by mutant is an important step in tumorigenesis. Method: 4 colon cancer cell lines (SNU C1, C2A, C4, C5) were examined for mutation in exons 5 to 8 of the p53 tumor suppressor gene by PCR-SSCP analysis and expression pattern by western blotting and immunoprecipitation. p53-mediated transactivation ability were examined by CAT assay and base substitution of p53 in SNU C2A cell were detected by DNA sequencing. Results: 1) SNU C2A cell and SNU C5 cell were detected mobility shifts each in exon 5 and exon 7 of p53 gene by the PCR-SSCP method, implicating being of p53 mutation. 2) 3 colon cancer cell lines (SNU C1, SNU C2A, SNU C5) expressed wild type and mutant type p53 protein. 3) In northern blot experiment, SNU C2A and SNU C5 cell expressed high level of p53 mRNA. 4) Results of p53-mediated transactivation in colon cancer cell lines by CAT assay represented only SNU C2A cell has transcriptional activity. 5) DNA sequencing in SNU C2A cell showed missense mutation in codon 179 of one allele, histidine to arginine and wild type p53 in the other allele. Conclusion: Colon cancer cell lines showed correlation with mutation in p53 gene and accumulation of abnormal p53 protein. Colon cancer cell SNU C2A retained p53-mediated transactivation as heterozygous p53 with one mutant allele in 179 codon and the other wild-type allele.

응급구조학과 학생들의 재난대처핵심역량과 재난대처방법에 대한 인식

정지연,Jung, Ji-Yeon 한국응급구조학회 2018 한국응급구조학회지 Vol.22 No.2

Purpose: To investigate the perception of paramedic students about core competence and methods of disaster response. Methods: A self-reported questionnaire was completed by 210 paramedic students in Jeollado from March 2 to April 2, 2018. It comprised 57 questions on topics such as general characteristics (6), related experience (11), cognition of core competence (15), and knowledge of disaster response methods (25). Data were analyzed by means of a frequency analysis, a t-test, an ANOVA, and the Pearson's correlation coefficient using SPSS WIN 21.0. Results: The average score of the participants on disaster response core competence was 3.21. The statement "In case of a disaster, I know the duties of medical staff" scored the second highest, at 3.58, and "In the case of disaster, I can conduct the basic treatment" scored the highest ar 3.59. The average score for the disaster response methods was 3.31. Within the section, the statement "I know what I need to do in the case of burn patients" scored 3.59 and "I know what I need to do in the case of a cardiac arrest patient" scored the highest at 3.93. Conclusion: It is necessary to develop a training module considering the diverse types of disasters and a standardized education program for simulation similar to real-life situations.

새만금 방조제 외해역의 저서생물 군집 변화

정지연,권효근,장정렬,최강원,Jung, Ji-Yeon,Kwon, Hyo-Keun,Jang, Jeong-Ryeol,Choi, Kang-Won 한국관개배수위원회 2010 한국관개배수논문집 Vol.17 No.2

This study was carried out to investigate the changing of benthic macrobenthos community in Saemangeum subtidal area. The distribution of macrobenthic community is important to assume the benthic environment in study area. Macrobenthic community was investigated three times in 2007 (March, June and September) in the Saemangeum subtidal area. The mean density of macrobenthos was $345{\sim}838ind./m^2$ and the most dominant taxa was benthic polychaete. Opportunistic species such as E.cordatum, T.fragilis, H.filiformis, and Tharyx sp. were shown high appearance rate in study area. The dominance of these opportunistic species suggest that the benthic environment of study area was unstable due to environmental disturbance. But, there are only few point that these species were recorded high densities, and also equilibrium species was dominant, too. It means disturbance and un stability of benthic environment in Seamangeum was not serious yet. so the dynamics and trends of these opportunistic species should be concerned.

논문 - HSPF를 이용한 새만금 유역의 오염부하 특성

정지연,신유리,최정훈,최인규,윤춘경,손영권,Jung, Ji-Yeon,Shin, Yu-Ri,Choi, Jung-Hoon,Choi, In-Kyu,Yoon, Chun-Gyeong,Son, Yeong-Kwon 한국관개배수위원회 2011 한국관개배수논문집 Vol.18 No.2

This study was performed to analyze the influence of pollutant loads characteristics on the point and non-point sources in Saemangeum watershed area using Hydrological Simulation Program, Fortran (HSPF). The simulation items were flow, BOD, T-N, and T-P(2007~2010). The pollutant loads trend reflects the precipitation. Specifically, the point source loads were almost constant, but the non-point source loads were influenced in the precipitation. It was found that the effect of non-point source is larger than point source. The water quality had a clear trend by the season. However, pollutant loads did not show distinct seasonal changes. The reason is that the pollutant concentration is diluted by the increased flow at summer season. Therefore, it is important to control the non-point source in order to manage water quality in the region. For the management of Saemangeum lake, it is need to control of supplied pollutant loads from Saemangeum watershed.

토끼에서 Pasteurella multocida 감염에 의한 뇌막뇌염과 폐렴 증례보고

정지연,이경현,최은진,김하영,손준형,소병재,정지열,Jeong, Jiyeon,Lee, Kyunghyun,Choi, Eun-Jin,Kim, Ha-Young,Sohn, Jun Hyung,So, ByungJae,Jung, Ji-Youl 대한수의학회 2018 大韓獸醫學會誌 Vol.58 No.1

Eight rabbits exhibited head tilt and subsequently died. At necropsy, three rabbits had crusty deposits in ears and four had reddish lungs. The main histopathological features were severe diffuse suppurative meningoencephalitis (75.0% of rabbits), fibrinopurulent pneumonia (37.5%), and otitis externa (37.5%). Pasteurella multocida (P. multocida) was isolated from brains, ears, and lungs. The capsular serogroups of the isolates were untypable. Based on histopathological features and bacterial analysis results, the rabbits were diagnosed as P. multocida infection. P. multocida infections might result in considerable economic loss in commercial rabbit production facilities in Korea.

일반인의 심폐소생술 인식에 따른 교육 활성화 방안 - 전북지역을 중심으로 -

정지연,심정신,신상열,Jung, Ji-Yeon,Shin, Chung-Sin,Sin, Sang-Yol 한국응급구조학회 2007 한국응급구조학회지 Vol.11 No.3

This study was attempted to provide basic data develop CPR training program for layperson by looking into layperson's recognition and attitude of execution of CPR and to prepare for underlying data in drawing up training policy and suggesting relevant legislation so that trained laypersons can positively perform rescue activities. The survey was done from August 20 to September 20, 2007. Total Subjects in this study were 78. The collected data were analyzed by SPSS Program. The summary of the research is as follows : First, to the question of whether or not the recognition or performance of CPR is universal within the country, 82.1% of respondents gave a negative answer as 'No', The most reason was found to be 'Poor training and P.R. of CPR' accounting for 50.1%, 94.8% of the whole respondents answered that CPR training is necessary. As plan for activatin of CPR training, they answered that top priority shall be given to compulsory school training(79.5%). Secondly, when respondents observed their family's cardic 84.6% of them answered that they would conduct CPR but when they observed other's cardiac arrest, just 41.7% of them answered it. As an effective CPR activation plan, the most answer was training and P.R. of CPR as 79.5%. This study suggests that laypersons high perception of the effectiveness of the CPR and that they wound be willing to provide CPR in a medical emergency.

개방형 IPTV 환경에서의 사용자 인증 및 키 분배 메커니즘

정지연,도인실,채기준,Jung, Ji-Yeon,Doh, In-Shil,Chae, Ki-Joon 한국정보처리학회 2011 정보처리학회논문지 C : 정보통신,정보보안 Vol.18 No.4

IPTV는 대표적인 방송통신융합 산업으로 IP망의 양방향성을 이용한 서비스의 차별화를 내세우며 여러 사업자에 의해 서비스가 이루어지고 있다. 최근에는 모바일 환경 등으로 그 영역을 확대하기 위한 연구가 진행되고 있으며 다른 한편으로는 IPTV 서비스를 위한 플랫폼 등을 개방하여 사업자가 아닌 일반인도 IPTV를 통한 방송을 할 수 있는 구조인 개방형 IPTV에 대한 연구가 활발하다. 이러한 개방형 IPTV 환경에서는 다수의 콘텐츠 제공자가 존재하기 때문에 기존 IPTV에서 사용되는 특정 기기 혹은 스마트카드를 통한 사용자 인증 및 키 분배가 어려운 실정이다. 본 논문에서는 기존 분산 네트워크에서의 인증 메커니즘인 Kerberos를 기반으로 하여 분산 환경에서 안전한 사용자 인증과 키 분배를 위한 메커니즘을 제안하였다. 제안 메커니즘은 콘텐츠의 안전한 전송을 보장하며 기존 제안된 IPTV 사용자 인증 관련 연구보다 인증 시간 오버 헤드에 있어 우위에 있음을 시뮬레이션을 통해 증명하였고 다양한 관점에서 제안 메커니즘을 다른 관련 연구와 비교하여 개방형 IPTV 환경에서 제안 메커니즘이 갖는 효율성을 증명하였으며 IPTV가 갖는 보안 요구 사항을 제안 메커니즘이 만족한다는 것을 보였다. IPTV(Internet Protocol Television) is one of the typical businesses which are the convergence of Broadcast and Communication. It provides broadcasting service using IP networks. Recently, IPTV service is developed to Mobile IPTV or Open IPTV. Especially, Open IPTV uses open platform so not only service providers but also general users can provide contents to other users. Open IPTV system has many content providers, so existing security solution of IPTV cannot be adopted. In this paper, we suggest user authentication and key distribution mechanism on Open IPTV. Our proposed mechanism is based on Kerberos, so it can support distribution environment such as Open IPTV. We demonstrate that proposed mechanism can guarantee secure transmission of contents and reduce the delay of user authentication on Open IPTV system compared to other authentication mechanisms. We also compare our proposal and other mechanisms in various aspects, and analyze efficiency and safety of proposed mechanism. As a result, we insist that this mechanism satisfies the security requirements for IPTV.

강원학 아카이브 구축에 대한 이론적 연구

정지연(Jung, Ji-Youn) 강원대학교 인문과학연구소 2019 인문과학연구 Vol.0 No.62

지역학에 대한 관심이 높아지면서 강원도와 관련된 수많은 자료를 한 곳에 모아 집적(集積)하고 체계적으로 관리·활용할 수 있는 아카이브 구축에 대한 필요성이 증대되고 있다. 강원학 관련 자료를 총괄하여 집적하고 이를 서비스 하는 아카이브를 구축한다면, 연구자들이나 일반인들이 강원학 관련 자료에 대한 접근성이 확대됨으로써 이를 기반으로 강원학 연구의 활성화를 기대할 수 있다. 뿐만 아니라, 구축된 자료를 교육, 연구, 창작 산업 등 다양한 자원의 콘텐츠 개발 및 응용을 위한 원천 기반을 제공함으로써 자료가치를 재생산할 수 있다. 강원학 아카이브를 구축하기 위해서는 강원도 관련 자료에 대한 종합적이고 체계적인 정리 및 DB화 방안, 저작권 문제, 자료 제공에 대한 표준화 방안 등 여러 가지 해결하여야 할 문제점이 있기 때문에 이를 위한 정밀한 설계가 먼저 선행되어야 한다. 이에 본 연구는 강원학 관련 자료의 방대함과 자료 유형의 다양성 등을 고려하여, 수집·관리·활용의 측면에서 강원학 아카이브 구축을 위한 기본 요소를 분석하고, 단계별 전략을 마련하여 향후 강원학 아카이브 구축을 위한 로드맵을 제안하고자 하였다. 또한, 강원학 아카이브 구축이 실효성을 가지기 위해서는 수집 정책 수립, 행·재정적 지원, 협의체 구성 등을 선결과제로 제시하였다. With increasing interests in regional studies, it is necessary to construct an archive to collect the many resources related to Gangwon Province and to manage and use them systematically. An archive that can steer, collect, and service the resources related to Gangwon Studies would expand the accessibility of resources related to Gangwon Studies for researchers or the public and lead to more studies on Gangwon. Not only that, the value of resources may be reproduced by providing the constructed resources for the development and application of various resources in education, research, creative industries, and more. In order to construct the Gangwon Studies Archive, there are some problems to resolve, such as comprehensive and systemized organization and database construction of resources related to Gangwon Province, copyright issues, and standardization of resources, and it is necessary to develop a precise design. The current study considered the expansiveness of resources related to Gangwon Studies and the diversity of resources to analyze the basic elements of building an archive in terms of collection, management, and utilization and establish step-by-step strategies to suggest a road map for the construction of Gangwon Studies Archive. In order for Gangwon Studies Archive to take effect, it is necessary to establish collection policies, provide administrative/financial support, organize a council, etc.

근현대기록물 수집활동 체계화 방안

정지연(Jung Ji Youn) 강원대학교 인문과학연구소 2007 江原人文論叢 Vol.18 No.-

근현대기록물은 다양한 매체를 통해 근현대시기의 모습을 전달해주는 중요한 사료적 가치를 지니고 있으나, 기록물이 산재해 있고, 인식의 부재로 인한 관리소홀로 사장되어 갈 위기에 놓여 있어 근현대 기록물의 관리를 위한 체계적 수집이 시급하다. 이러한 문제 의식하에 근현대기록물을 수집하고 있는 국사편찬위원회의 수집활동에 대한 사례분석을 통해 다음과 같은 문제점을 확인할 수 있었다. 첫째, 근현대 기록물 수집대상에 전근대기록물도 포함시키고 있어 수집전반에 혼선을 야기한다. 둘째, 다양한 유형의 기록물 수집이 기대됨에도 불구하고 여전히 문서중심의 기록물이 수집되고 있다. 셋째, 수집된 기록물의 이용 등을 위해 필요한 관할권을 확보하지 않고 있다. 넷째, 수집된 기록물 중 불필요한 기록물에 대한 재처리 방안이 없다. 이러한 문제에 대한 개선방안으로 아래와 같은 사항을 제시하였다. 첫째, 국편의 수집정책을 수립하여 성문화한다. 기관과 관련된 요소, 수집대상과 관련된 요소, 관리와 관련된 요소, 정책과 관련된 요소 등의 네 가지 필수요소를 통해 기관의 사명과 목적에 맞는 수집정책을 수립함으로써 체계적이고 효율적인 기록물 수집을 이룰 수 있을 것이다. 둘째, 법적 권한의 확보이다. 수집된 기록물을 보존하고 이용하기 위해서는 기록물에 대한 법적 권한을 확보해야 한다. 가장 대표적인 예로 기증 증서의 작성이 있지만, 국편의 경우 기록물 수집에 있어 기증이나 기탁, 위탁 등의 방법보다는 복사 및 복제의 방식으로 수집하고 있으므로, 이후 기록물의 이용이나 전시 등의 활용에 대한 설명 및 합의 절차는 반드시 거쳐야 할 것이다. 셋째, 기록물 유형에 맞는 수집방법의 고안이 요구된다. 근현대기록물은 문서 형식은 물론, 사진이나 필름 등의 시청각 기록물과 박물류등의 다양한 유형의 기록물이 수집 가능함에도 불구하고 기록물 유형에 따른 수집방안이 설립되지 않고 있다. 따라서 국편은 현재까지 수집된 기록물의 유형을 파악하고 잠재된 기록물 유형을 설정하여 그에 대한 기록물 유형별 수집방법을 마련해야만 한다. 또한 기록물 유형에 맞는 목록양식의 고안도 필요할 것이다. Modern documentary has important values that can notify the Japanese Imperialist's rule in the Korean Peninsula, liberation from the Japanese Imperialist's rule and the Korean War, etc through various kinds of media. The documentary can be found here and there to manage it carelessly because of low cognition and to be likely to keep in dead storage, so that it should be systematically collected to manage it effectively. The study has investigated cases of collection activities of the National Institute of Korean History to find out problems and to suggest reformation programs. The paper has set up definition as well as scope of the documentary collection that is theoretical background, and it has examined not only basic factors of the collection but also requirements at each stage of the collection. The paper investigated collection activities of modern documentary. The findings were as follow: Firstly, the target of collection of modern documentary has included premodern documentary to disturb all of collection activities. Secondly, various kinds of documentaries are expected to collect: But, only document type of documentary has been collected. Thirdly, jurisdiction for the use of the documentary collected has not been obtained. Fourthly, there is no reprocessing program of unnecessary documentary. The paper has suggested reformation of the problems as follow: Firstly, the collection policy of the National Institute of Korean History is to be established to put in statutory form. The National Institute of Korean History shall establish collection policy through four factors, that is to say, organization, collection object, management and policy, etc to satisfy mission and purposes and to collect documentary systematically and effectively. Secondly, the legal authority shall be obtained. The legal authority of documentary shall be obtained to keep and make use of the documentary collected. For instance, a certificate of donation can be used. The National Institute of Korean History has collected documentary not by donation, deposition and consignment, etc but by copying and reproduction, so that explanation and agreement on the use of documentary and exhibition shall be required. The process can guarantee rights and responsibility of not only owners of the documentary but also the National Institute of Korean History. Therefore, explanation and agreement of the use of the documentary collected shall put in a statutory form to obtain legal rights of the documentary. Thirdly, collection method that can meet types of the documentary is required. Even if modern documentary can be collected in various types, for instance, document, photographs, films and other audio-visual materials, and various kinds of objects, collection method has not been made depending upon types of documentary. Therefore, the National Institute of Korean History shall investigate types of the documentary collected to set up types of potential documentary and to make collection method depending upon types of the documentary. And, a list form that can meet types of the documentary shall be required. The paper has examined cases of the National Institute of Korean History to suggest reformation of the problems and to produce basic material that can establish standard collection method of modern documentary in the future.

디지털 시대의 영화 존재론에 관한 연구: 매체 융합환경에서 영화의 수용자 경험 변화를 중심으로

정지연 ( Ji Youn Jung ) 한국커뮤니케이션학회 2009 커뮤니케이션학연구 Vol.17 No.1

본 연구는 영화의 존재론(Ontology), 즉 영화(Cinema)가 무엇인가 하는 것을 연구한 논문이다. 영화는 1895년 처음 등장했고, 이후 백여 년이 넘는 시간동안 역사적, 지역적, 기술적, 산업적, 미학적 특수성에 따라 다양한 모습으로 변모해왔다. 그러나 이 논문에서 주목하는 것은 1990년대부터 가시화된 디지털 테크놀로지와 영화의 문제이다. 디지털 테크놀로지는 영화의 지난 역사, 그 어떤 시기의 기술적 변화보다도 크고 강력하게 영화에 침투하며 영화의 존재양식에 변화를 주도하고 있다. 생산의 메커니즘은 물론이고 관객이 영화를 경험하는 방식 역시 전통적인 영화 경험과는 다른 양식(mode)으로 나아가고 있다. 고전적인 영화의 존재론은 영화가 극장의 예술이며, 사회적 제도이자 경험이라는 것을 전제하고 있다. 그러나 디지털 컨버전스 미디어가 만들어낸 영화의 다양한 형태들(극장을 포함하여, TV, 컴퓨터, DVD, PMP 등)은 전통적인 영화의 경험 양식을 공적, 집단적, 강제적, 선형적, 이벤트적 성격으로부터 사적이고 개인적, 비선형적, 선택적, 즉흥적인 것으로 변모시키고 있다. 그렇다면 디지털 시대에 이렇듯 변모하는 성격을 지닌 영화를, 과거 우리가 불렀던 전통적인 영화(cinema)와 과연 같은 것이라고 말할 수 있을까? 이를 위해 본고에서는 디지털 시대 영화의 현재적 특성을 이해하기 위해 영화 고고학(archeology)의 방법론을 채택하고, 과거 역사적 경험 속에서 발견되는 영화의 다양한 특징과 제도들을 현재적인 것과의 상관관계 속에서 살펴보았다. This study is on the ontology of cinema, asking what cinema is. Cinema first appeared in 1895 and has changed itself a lot in accordance with its historical, regional, technological, industrial and aesthetic specificity. However, what this study tries to pay attention to is especially the mutual relationship between the digital technology and cinema, which has been built since the 1990s. In fact, digital technology penetrated powerfully into cinema than any other technological changes in the past and has been leading the shift in cinema`s ontological mode. Due to digital technology, the mechanism of production as well as the mode of experience of cinema develops itself in a different way from the traditional mode of it. In other words, the classical ontology of cinema premises cinema as an art of theatre as well as a social institution and experience, while various forms of cinema resulted from the digital convergence media such as TV, computer, DVD and PMP, not to mention the theatre, alter the traditional mode of experience of cinema: from the public, collective, forcing, linear and event-like characteristics to the private, individual, nonlinear, selective and spontaneous ones. Thus, the following question was the biggest issue in this study: can we categorize this newly changing cinema in the digital era as the same as the traditional cinema? In order to answer this question, I would like to employ the methodology of cinematic archeology to understand the present characteristics of digital cinema, and explored the various characteristics and institutions found in the past historical experiences in a relationship with the present ones.