K562 세포주에서 Genistein에 의해 억제되는 Radiation-induced Apoptosis의 조절 유전자

정수진,진영희,유여진,도창호,정민호,허기영,배혜란,양광모,문창우,오신근,허원주,이형식,Jeong, Soo-Jin,Jin, Young-Hee,Yoo, Yeo-Jin,Do, Chang-Ho,Jeong, Min-Ho,Huh, Gi-Yeong,Bae, Hye-Ran,Yang, Kwang-Mo,Moon, Chang-Woo,Oh, Sin-Geun,Hur, Won-Jo 대한방사선종양학회 2001 Radiation Oncology Journal Vol.19 No.3

목적 : K562 세포를 대상으로 PTK inhibitor (herbimycinA와 genistein)에 의한 방사선 유도 apoptosis의 변화에 따른 관련 유전자를 탐색하고자 하였다. 대상 및 방법 : K562 세포는 $2\times10^5\;cells/mL$의 비율로 준비하여 대수증식기로 성장한 세포를 각각의 실험조건에 따라 처리하여 사용하였다. 방사선 조사는 6-MV X-Ray 10 Gy (Clinac 1800C, Varian, USA)를 $200\~300\;cGy/min$의 선량율로 상온에서 일정하게 조사하였다. Herbimycin A (HMA, Calbiochem, UK)와 genistein (Calbiochem, UK)은 dimethylsulfoxide (DMSO, Sigma, UK)에 녹여서 각각 1 mM과 10 mM의 농축용액으로 조제한 각각 250 nM과 $25\;{\mu}M$의 최종농도로 처리하였다. 내성 변화의 조절에 관여하는 유전자를 찾고자 PCR-select cDNA subtractive hybridization을 실시하여 128개의 차별 발현되는 clones을 재선별하였다. DNA sequencing을 통하여 염기서열을 분석하였으며, GenBank database를 이용하여 이미 밝혀진 유전자들과의 상동성을 비교하였다. 상동성을 갖는 clone을 확인하여 이를 probe로 사용하여 방사선 단독조사한 실험군과 방사선과 HMA 또는 genistein을 동시 처리한 실험군들간의 mRNA 발현의 차이를 Northern hybridization을 통하여 확인하였다. 결과 : homo sapiens Smad6 유전자와 $95\%$의 상동성을 갖는 clone을 확인하였다. 이를 probe로 사용하여 방사선 단독 조사한 실험군과 방사선과 HMA 또는 genistein을 동시 처리한 실험군들간의 mRNA 발현의 차이를 비교 분석한 결과, 방사선과 genistein을 동시 처리한 실험군의 mRNA 발현이 방사선 단독 조사한 실험군과 방사선과 HMA를 처리한 실험군들에 비하여 현저히 높았다. 결론 : Smad6와 방사선에 의해 유도되는 apoptosis의 억제에 관한 연관성을 보고한 문헌은 없으나, Smad6가 일부세포에서 apoptosis를 억제한다는 보고들과, 본 연구에서 관찰한 genistein에 의한 방사선에 의해 유도되는 apoptosis의 억제에서 그 발현이 두드러지게 증가한 점 등을 미루어 보아 방사선에 의하여 유도된 apoptosis의 억제 및 방사선 내성의 조절에도 관여할 것으로 생각된다. Prupose : The genes involved on the suppression or radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated. Materials and methods : K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. For X-ray irradiation and drug treatment, cultures were prepared at $2\times10^5\;cells/mL$. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA), Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at $37^{\circ}C$ for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined. Results : Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis. Conclusion : We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.

냉간시동시 자동차용 저온활성촉매의 성능 향상을 위한 수치적 설계

정수진,김우승,Jeong, Soo-Jin,Kim, Woo-Seung 대한기계학회 2000 大韓機械學會論文集B Vol.24 No.9

Light-off catalyst has been used for minimization of cold-start emissions. Improved cold-start performance of light-off catalyst needs the optimal design in terms of flow distribution, geometric surface area, precious metal loading, cell density and space velocity. In this study, these influential factors are numerically investigated using integrated numerical technique by considering not only 3-D fluid flow but also heat and mass transfer with chemical reactions. The present results indicate that uneven catalyst loading of depositing high active catalyst at upstream of monolith is beneficial during warm-up period but its effect is severely deteriorated when the space velocity is above 100,000 $hr^{-1}$ To maximize light-off performance, this study suggests that 1) a light-off catalyst be designed double substrate type; 2) the substrate with high GSA and high PM loading at face be placed at the front monolith; and 3) the cell density of the rear monolith be lower to reduce the pressure drop.

자동차용 촉매변환기의 최적설계를 위한 열 및 유동특성에 대한 수치적 연구

정수진,김우승,Jeong, Soo-Jin,Kim, Woo-Seung 대한기계학회 1999 大韓機械學會論文集B Vol.23 No.7

In the present work, the effect of a flow maldistribution on the thermal and conversion response of 8 monolith catalytic converter is Investigated. To achieve this goal, a combined chemical reaction and multi-dimensional fluid dynamic mathematical model has been developed. The present results show that flow uniformity within the monolith brick has 8 great impact on light-off performance of the catalytic converter. In the case of lower flow uniformity, large portions of the monolith remain cold due to locally concentrated high velocities and CO, HC are unconverted during warm-up period, which loads to retardation of light-off. It has been also found that the heat-up pattern of the monolith ill similar to the flow distribution profile, In the early stage of the reaction. It may be concluded that flow maldistribution can cause a significant retardation of the light-off and hence can eventually worsen tho conversion efficiency of automotive catalytic converter.

사료내 미량광물질 강화 복합미생물 첨가가 산란노계의 계란생산 및 난질개선에 마치는 영향

정수진,주은정,이우식,윤병선,이주삼,남기택,황성구,Jeong, Soo-Jin,Joo, Eun-Jung,Lee, Woo-Sic,Yon, Byeng-Sun,Lee, Ju-Sam,Nam, Ki-Taek,Hwang, Seong-Gu 한국유기농업학회 2004 韓國有機農業學會誌 Vol.12 No.2

In recent years, the consumption of livestock products were markedly decreased by awareness of world-widely occurred diseases including mad cow disease, Foot and mouth disease, Hog cholera, and Poultry Influenza virus. the consumers ara also wanting to have highly safe food such as organic animal products because of concerning about residual of antibiotics in animal products. However, disease control and impairment of productivity are the major problem in organic animal production. On these points of view, the present study was undertaken to investigate the effects of 1% or 2% of dietary probiotics fortified with various minerals on improvement of egg production and egg quality in old lay6r feeding low quality feed mainly composed of food waste, sesame meal, and rice bran. After 4 weeks of experimental feeding, the diameter of spreading of egg white was clearly decreased from 11.2cm of control eggs to 10.5m and 10.1m in 1% and 2% treatment group eggs, respectively. The color of egg yolk was 9.3 in control eggs but remarkably increased in treatment groups showing 10.1~10.2. Egg production was 75.8% in control layers but significantly increased to 79.8% of 1% treatment group and 82.9% of 2% treatment group layers. Egg weights (C : 66.3g, 1% : 73.2g, and 2% : 76.7g) and egg shell thickness (C : 0.33mm, 1% : 0.35mm and 2% : 0.36mm) were also increased by the addition of 1% or 2% of probiotics when compared to those of control group eggs. All together, it has been suggested that dietary addition of probiotics fortified with various minerals can improve the egg quality and egg production in layer's productivities by the recycling of organic waste resources such as food waste and agricultural by-products.

커먼레일 연료분사 시스템을 장착한 경량 DME 트럭의 연구 및 개발

정수진,전문수,박정권,Jeong, Soo-Jin,Chon, Mun Soo,Park, Jung-Kwon 한국교통대학교 융복합기술연구소 2012 융ㆍ복합기술연구소 논문집 Vol.2 No.1

In this study, the trucks(2.9-liter) have been developed to use DME as fuel, and performance test of the vehicle's DME engine, power, emissions, fuel economy and vehicle aspects was conducted. For experiments, the fuel system(common-rail injectors and high-pressure pump included) and the engine control logic was developed, and ECU mapping was performed. As a result, the rail pressure from 40MPa to approximately 65% increase compared to the base injector has been confirmed that. Also, the pump discharge flow is 15.5 kg/h when the fuel rail pressure is 400rpm(40 MPa), and the pump discharge flow is 92.1 kg/h when the fuel rail pressure is 2,000rpm(40MPa). The maximum value of full-load torque capability is 25.5kgfm(based on 2,000rpm), and more than 90% compared to the level of the diesel engine were obtained. The DME vehicle was developed in this study, 120 km/h can drive to the stable, and calculated in accordance with the carbon-balance method of fuel consumptions is 5.7 km/L.

건강 진단 수검 여성들의 유방 자가 검진에 대한 인식도 및 실천정도에 관한 연구

정수진,최순석,박준한,엄상화,정귀원,이채언,배기택,Jeong, Soo-Jin,Choi, Soon-Seok,Park, Jun-Han,Urm, Sang-Hwa,Jeong, Kui-Won,Lee, Chae-Un,Bae, Ki-Taek 대한예방의학회 1998 예방의학회지 Vol.31 No.4

The methods for the early diagnosis of breast cancer are mammography, physical examination, breast self-examination(BSE). This study was conducted to investigate the knowledge and practice of BSE by structured questionnaire which included questions on general characteristics, performance of mammography, physical examination of breast, knowledge and practice of BSE. The subjects in this study were 412 females between 20 and 69 year old who visit to the Health Care Center of College of medicine of Inje University from December 1997 to February 1998. The results were as follows. 1. Women who have Soften the regular mammography are 8.19% of these subjects and women who have gotten the regular physical examination by doctors are 4.19% of these subjects. 2. Regarding BSE, 77.4% of these subjects said that they knew BSE and 3.88% of these subjects said that they practiced BSE regularly. It was that women who knew BSE were more younger, better-educated and higher economic status(p=0.001) and lived a normal life with he. husband(p=0.033). And women who practiced BSE regularly were more younger, and married(p=0.001). 3. The most common barrier to doing BSE is 'Do not see the needs/worry unnecessarily', and the most common reasons for doing BSE is 'Early detection of Breast cancer'. 4. The overall frequency and percentage distribution of recommended BSE step in Korea is lower than U.S.A.

대형 디젤엔진용 Urea-SCR 시스템의 NH₃슬립 억제를 위한 최적 인젝터의 형상 및 위치에 관한 수치적 연구

정수진(Soo-Jin Jeong),이상진(Sang-Jin Lee),김우승(Woo-Seung Kim) 한국자동차공학회 2004 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

In the past few years, Considerable efforts have been directed towards the further development of urea-SCR (selective catalytic reduction) technique for diesel-driven vehicle. Although urea possesses considerable advantages over ammonia in terms of toxicity and handling, its necessary decomposition into ammonia and carbon dioxide complicates the DeNOx process. Hence the keys to implementation of SCR technology is speeding up thermolysis, good mixing of ammonia and gas and reducing NH₃ slip. In this context, this study performed three-dimensional numerical simulation of urea injection of heavy-duty diesel engine under various conditions of injection pressure, location and injector hole number. The results of this study show some guidelines about the optimum locations and shape of urea injector for reducing NH₃ slip and improving DeNOx performance.

장수명 주택의 수선교체주기 설정을 위한 BIM활용에 관한 연구

정수진 ( Jeong Soo-jin ),박정로 ( Park Jung-lo ),김주형 ( Kim Ju-hyung ),김재준 ( Kim Jae-jun ) 한국건축시공학회 2011 한국건축시공학회 학술발표대회 논문집 Vol.11 No.2

제어알고리즘에 따른 Urea SCR 분사 시스템의 NOx 및 NH₃ 배출 특성

정수진(Soo-Jin Jeong),이호길(Ho-Kil Lee),오세두(Se-Doo Oh),박정권(Jung-Kwon Park),김영득(Young-Deuk Kim),심성민(Sung-Min Shim),김우승(Woo-seung Kim) 한국자동차공학회 2009 한국자동차공학회 학술대회 및 전시회 Vol.2009 No.11

The selective catalytic reduction(SCR) system is a highly-effective aftertreatment device for NOx reduction of diesel engines. Generally, the ammonia(NH₃) was generated from reaction mechanism of urea solution in the SCR system using the liquid urea as the reductant. Therefore, the precise urea dosing control is a very important factor for reducing NOx and NH₃ slip in the SCR system. This paper investigated that the effect of urea dosing control algorithms on NOx and NH₃ emission characteristics in order to minimize NOx emission and NH₃ slip. In the map-based control algorithm, the urea injection was determined by mass flow rate of exhaust gas based on engine rpm, torque and O₂, NOx concentration measured by NOx sensor. However, this algorithm cannot consider the effect of adsorption and desorption of NH₃ so that the amount of urea injection may be over- and under-estimated, which leads to NH₃ slip and deterioration of deNOx performance. Therefore, the model-based control algorithm based on physics of chemistry and mechanics has been developed in this study. The mass flow rate map obtained from engine test and NO, NO₂ and O₂ concentration measured by NOx sensor during engine test were used. A comparison of map-based and model-based control algorithms has been performed in terms of NOx emission and NH₃ slip

간 각질 세포 및 마우스 비장 세포를 이용한 방풍통성산의 항알러지 효능 연구

정수진(Soo-Jin Jeong),이미영(Mee-Young Lee),서창섭(Chang-Seob Seo),신현규(Hyeun-Kyoo Shin) 한의병리학회 2015 동의생리병리학회지 Vol.29 No.2