ATP에 의한 자궁경부암 세포내 NF-κB와 칼슘이온의 변화

임대석 ( Dae Seog Lim ),배수미 ( Su Mi Bae ),곽선영 ( Sun Young Kwak ),정국화 ( Guo Hua Ding ),김정 ( Jeong Kim ),김용욱 ( Yong Wook Kim ),노덕영 ( Duck Yeong Ro ),이준모 ( Joon Mo Lee ),남궁성은 ( Seung Eun Namkoong ),조영래 ( Yo 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.12

미생물 일반 , 생리 및 대사 Pyricularia oryzae 의 성장을 억제하는 물질을 생산하는 Streptomyces sp . NA - 52 의 분리 및 동정

윤원호,임대석,이명섭,김창한 ( Won Ho Yoon,Dae Seog Lim,Myung Sub Lee,Chang Han Kim ) 한국미생물생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.6

미생물 일반 , 생리 및 대사 항종양활성을 Enterococcus faecalis 2B4-1 의 분리 및 동정

박상진 ( Sang Jin Park ),김창한 ( Chang Han Kim ),백영진 ( Young Jin Baek ),임대석 ( Dae Seog Lim ),윤상군 ( Sang Kun Yoo ) 한국미생물생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.6

Near-infrared (NIR) 영상기법을 이용한 생체 내 수지상세포의 이동

이준호(Jun-Ho Lee),정남철(Nam-Chul Jung),이은계(Eun Gae Lee),임대석(Dae-Seog Lim) 한국생물공학회 2012 KSBB Journal Vol.27 No.5

Matured dendritic cells (DCs) begin migration with their release from the bone marrow (BM) into the blood and subsequent traffic into peripheral lymphoid and nonlymphoid tissues. Throughout this long movement, migrating DCs must apply specialized skills to reach their target destination. Non-invasive in vivo cell-tracking techniques are necessary to advance immune cell-based therapies. In this study, we used a DiD cell-tracking solution for in vivo dendritic cell tracking in naive mice. We tracked DiD (non-invasive fluorescence dye)-labeled mature dendritic cells using the Near Infrared (NIR) imaging system in normal mice. We examined the immunophenotype of DiD-labeled cells compared with non-labelled mature DCs, and obtained time-serial images of NIR-DC trafficking after mouse footpad injection. In conclusion, we confirmed that DiD-labeled DCs migrated into the popliteal lymph node 24 h after the footpad injection. Here, these data suggested that the cell tracking system with the stable fluorescence dye DiD was useful as a cell tracking tool to advance dendritic cell-based immunotherapy.

구제역 바이러스 혈청형 A와 O 에피토프 재조합단백질 백신의 구제역 방어 효능

박미나(Mi Na Park),이혜영(Hae Young Lee),이현수(Hyun Soo Lee),임대석(Dae Seog Lim),문상범(Sang Bum Moon) 한국생물공학회 2020 KSBB Journal Vol.35 No.4

Foot-and-mouth disease (FMD) is a highly contagious viral disease susceptible to cloven- hoofed animals such as cattle, pig and goat, consequently exerting a bad influence on the livestock industry. Although various FMD vaccines have been developed for several decades, the efficacy of current FMD vaccines is restrictive because of the FMD virus (FMDV) diversity. The aim of this study was to determine whether the FMD virus (FMDV) serotype A and O epitope recombinant protein vaccine shows protective effects in a FMD animal model using pigs. To manufacture the recombinant protein vaccine, three or six replicates of VP1 epitopes of A/Pocheon/SKR/2010 and O/Andong/SKR/2010 FMDV strains were used and coupled with a swine immunoglobulin G heavy-chain constant region. Pigs were vaccinated twice (interval of 4 weeks) intramuscularly and examined the serum titers of FMDV specific antibody and neutralization antibody. After FMDV challenge, the gross lesions and clinical signs in FMD were assessed, and the viremia was measured by RTPCR assay using oral swabs. In the field experiment using pigs, FMDV-specific antibodies were markedly induced in the sera of FMD vaccine-immunized pigs. In addition, produced antibodies dramatically inhibited FMDV in vitro. Moreover, from challenge test in pigs to test the potency of FMD vaccine, at least more than 93% of vaccinated pigs were clearly protected from FMD. Taken together, these results strongly demonstrate that FMDV bivalent epitope-constituted recombinant protein vaccine shows obviously protective effects against two different serotypes (A, O) of FMDV.

항진균성 항생물질을 생산하는 Streptoverticillium sp. NA-4083의 분리 및 동정

임대석,윤상균,이명섭,윤원호,김창한 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

전국 각지의 토양에서 441균주의 방선균을 분리한 결과 Trichophyton mentagrophytes, Trichophyton rubrum, Nanniza otae 및 Pyricularia oryzae 등의 진균에 광범위한 항진균활성을 나타내는 방선균 NA-4803을 선발하여 그 균주의 동정을 실시하였다. 분리균주 NA-4803은 Gram 양성세균 4균주, Gram 음성세균 2균주, 효모 2균주, 곰팡이 5균주, 조류 1균주에 대해 약한 항균활성을 보이는 반면, 곰팡이 Trichophyton mentagrophytes, Trichophyton rubrum, Nanniza otae 및 Pyricularia oryzae에 대해서는 비교적 강한 항진균활성을 나타내었다. 분리균주 NA-4803은 생리적 특성, 항균활성, lectithinase 및 lipolytic 활성, 각종 유기물의 분해력, 항생물질에 대한 저항성, 온도, pH 및 화학저해제의 첨가에 따른 생육, 질소원의 이용성 및 탄소원의 이용성 등에 있어서 cluster group F에 속하며 Cluster No. 58에 해당하는 Streptoverticillium blastmyceticum과 가장 유사하게 비교·동정되었으나 nitrate 환원성의 양성반응, L-tyrosine 분해력의 음성반응 및 cephaloridine에 대한 저항성에 음성반응을 보였고, 탄소원 중 L-rhamnose와 inulin을 이용하는 것으로 보아 Streptoverticillium blastmyceticum과 다른 특성을 나타내었다. 따라서 분리균주 NA-4803은 Streptoverticillium sp. NA-4803으로 동정되었다. The aim of the present research program was to develop a strain of actinomycetes producing antifungal substance. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 440 isolates, a strain (NA-4803) producing antifungal substance against Trichophyton spp. Nannizzia otae and Pyricularia oryzae was selected. The strain NA-4803 was identified as strain similar to Streptoverticillium blastmyceticum with respect to morphological and physiological characteristics, lecithinase and lipolytic activity, degradation of organic compounds, resistance to antibiotics and utilization of carbon and nitrogen sources. But it showed some differences such as positive reaction of nitrate reduction, negative reaction of L-tyrosine degradation, resistance to cephaloridine, and utilization of I rhamnose and inulin. The strain NA-4803 was named as Streptoverticillium sp. NA-4803.

Isolation and Physico-Chemical Properties of Cycloheximide-isomer from Streptomyces sp.NA-52

Yoon,Won-Ho,Lim,Dae-Seog,Kim,Chang-Han,Yoon,Sang-Kun 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

새로운 분리균주 Streptomyces sp. NA-52는 Pyricularia oryzae와 Chlorella regularis에 대해 항균활성물질을 생산하는 것으로 밝혀졌다. 그 균주에 의해 생산되는 항생물질 복합물 중 (compound A 및 B), 활성이 좋은 화합물 A가 분리되었으며, 그것의 이화학적 성질이 조사되었다. 그 화합물의 분자량은 281(EI-MS)로 결정되었고, UV, IR 및 ¹H, 13C NMR 스펙트럼 분석결과, cycloheximide(4-[2-(3, 5-dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione)의 이성체와 같은 glutarimide 그룹의 항생물질로 분류되었다. 한편, 백자무우 씨앗의 발아시험결과, compound A는 cycloheximide와 마찬가지로 백자무우씨앗에 대해 강한 독성을 가지고 있었으나, compound B는 그 씨앗을 100% 발아시켰다. 지금까지의 cycloheximide 이성체와 비교해 볼 때, compound B는 백자무우씨앗에 대해 전혀 독성을 나타내지 않는, glutarimide 그룹내의 새로운 cycloheximide 이성체로 추정되었다. 결과적으로 cycloheximide와 같은 glutarimide 그룹의 항생물질이 Streptomyces griseus가 아닌 다른 방선균종에서도 생산된다는 것으로 밝혀졌다. A new isolate of Streptomyces sp. NA-52 was found to produce antibiotics with antimicrobial spectra against Pyricularia oryzae and Chlorella regularis. The main active component, compound A, of the antibiotic complex (compounds A and B) produced by the strain was isolated, and its physico-chemical properties were investigated. The molecular weight of the compound was determined to be 281 (EI-MS). UV, 1R, and ¹H and 13C NMR spectra suggested that the compound is a cycloheximide (4-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione) isomerlike glutarimide group antibiotic. Ina germination test using BAIKJA radish seed, neither compound A nor cycloheximide germinated the seeds, but compound B completely germinated them. Compared with known cycloheximide-isomers, compound B may be thought of as a new cycloheximide-isomer, without toxicity for BAIKJA radish seed, in the glutarimide group.

항 Vancomycin 내성균성 항생물질을 생산하는 Streptomyces sp.AMLK-335의 배양조건

이기형,임대석,최경희,이경호,김창한,김정환 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-

본 연구는 새로운 항 vancomycin 내성균성 항생물질을 개발하기 위하여 실시되었으며, 전국 각지의 토양 시료로부터 분리한 방선균 중 vancomycin 내성균주인 K-98 Enterococcus faecium 642, K-98 Enterococcus faecium 637 균주에 대하여 항생물질을 생산하는 분리균주 AMLK-335를 선정하여 이 균주의 항생물질 생산에 대한 최적 발효 조건을 검토한 결과, 탄소원 첨가로는 glycerol(0.5%), 질소원 첨가로는 yeast extract(0.5%)가 가장 효과적으로 항생물질 생산에 이용되었고, 비타민을 첨가에 따른 항생물질 생산성의 차이는 크게 나타나지 않았으나, calcium pantothenate 첨가시 항균활성이 비교적 높아졌으며, 금속이온의 첨가는 K+, Pb+, Ba²+, NA²+ , Ca²+를 첨가하였을 때는 항생물질의 생산성이 비교적 높아졌으며, 그중 Ca²+ 첨가구가 가장 생산성이 높았으며, Fe²+ , Hg+의 금속이온을 첨가하였을 때는 항생물질 생산성이 감소하였으며, CaCO₃첨가는 비첨가구보다 높은 항생물질 생산성을 보였으나, 첨가량에 따른 영향은 대체적으로 없었다. pH의 변화에 따른 생산성은 전반적으로 pH가 항생 물질 생산에 영향을 미치지 못하였으나, pH 4.5∼6.0 부근에서는 비교적 높은 항생물질 생산성을보였으며, 통기량에 따른 항생물질 생산성은 통기량이 많을수록 항생물질 생산이 좋았다. 한편 배양기간에 따라 항생물질 생산성을 조사한 결과, 5일째에 가장 높은 항생물질 생산성을 나타내었으며, pH의 변화는 배양 초기에 하강하였다가 점차 상승하는 경향을 보였다. This study was carried out for examining antibiotic productivity of the strain Streptomyces sp. AMLK-335 producing anti-VRE antibiotic. According to different condition, the strain was cultured for 4 days at 27℃ and determined cell growth, pH value and antibiotic productivity using disk diffusion method. In the experiment, the strain Streptomyces sp. AMLK-335 was examined various culture conditions which was added 8 carbon sources, 12 nitrogen sources, 8 vitamins and 11 metal ions in each time and controlled concentration of CaCO₃, initial pH and aeration. From the studies of conditions for antibiotic production, antibiotic productivity was the best to use 0.5% glycerol as carbon source and 0.5% yeast extract as nitrogen source. When several vitamins were added to medium, there was little difference in antibiotic productivity. But the addition of calcium pantothenate slightly increased antimicrobial activity. The addition of metal ion, such as K+, Pb+, Ba²+, NA²+ and Ca²+ slightly increased antibiotic productivity except Fe²+ and Hg+. The addition of CaCo₃showed higher than non-addition experiment in antibiotic production, but there wasn't generally influenced at addition concentration. According to pH change, antibiotic productivity was increased at pH 4.5∼6.0, and when there was a lot of aeration, antibiotic productivity was increased.