http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
BHA 와 NaCl 의 혼합첨가에 의한 미생물의 생육저해효과
윤원호,양종범,김창한 ( Won Ho Yoon,Jong Beon Yang,Chang Han Kim ) 한국축산학회 1985 한국축산학회지 Vol.27 No.1
These experiments were carried out to investigate the growth inhibition of Staphylococcus aureus, Escherichla coli and Pseudomonas fluorescens, respectively, in nutrient broth, and total viable cell counts in meat, using combination of butylated hydroxyanisole(BHA) and sodium chloride(NaCl). The results obtained were summarized as follows: 1. In the bacteria, Staphy. aureus was the most sensitive especially, bactericidal effect was shown at 200ppm BHA. With 5%-, 7%-NaCl and 50ppm BHA, a bacteriostatic effect was observed at 18, 12hr, respectively. 2. Cell numbers of E. coli increased at 200ppm BHA in the absence NaCl, but bactericidal effect was perceived at 5%-NaCl and 200ppm BHA, as well as, 7%-NaCl and 100ppm BHA. 3. The effect of bactericide on Ps. fluorescens was not present at below 200ppm BHA without NaCl, but at any level of BHA with above 5%-NaCl. 4. The addition of BHA and NaCl was effective in the prevention of total viable cell counts in raw meat (pork).
尹源鎬 전북대학교 1994 공산권연구 Vol.4 No.-
본문에서는 1978년 이래의 중공의 가격개혁에 초점을 맞추어 주로 개혁 전의 가격관리 체제의 폐단, 4단계 개혁의 주요 내용 및 미래에 대한 전망을 다루었다. 주기적인 경제 과열을 철저히 배제하기 위하여 중공은 개력을 심화하고 근본적으로 경제체제, 심지어는 정치상의 문제와 폐단을 척결해야 할 것이다.
티벳산 발효유로부터 분리한 Candida kefyr TFP 7의 항균활성 및 항암활성
윤원호,나영미,김창한 한국축산식품학회 2003 한국축산식품학회지 Vol.23 No.1
티벳산 발효유로부터 분리한 Candida kefyr TFP7의 항균활성 및 항암활성을 검토하기 위하여 PDA배지를 이용한 십진 희석법에 의해 10균주(TFP1∼10)를 분리하여 몇 종의 병원균과 식중독균, 식품과 관련이 있는 세균, 효모, 곰팡이, 녹조류 및 토양으로부터 분리한 방선균 등 18균주에 대하여 항균활성을 조사하였으며, 또한, 9종류의 인체 암세포주에 대하여 항암활성을 비교하였다. 항균활성 검사에서는 Gram 양성균 중 iwicrococcus iuteus ATCC l1880에 대하여 분리균주 (TFP2∼10)와 방선균인 Styeptomyces murinus JCM 4333에 패하여 분리균주(TFP6∼10)가 뚜렷한 생육억제효과를 나타내었다. 한편, 항암활성 검사에서는 SNU-5(human stomach carcinoma), SW-534(human larynx carcinoma)에 대하여 분리 균주(TFP1∼10) 모두 각각 60%, 70%이상의 생육억제 효과를 나타내었다. 특히, 균주 TFP7은 SNU-5, SW-534에 대하여 각각 77.5%, 76.5%의 가장 우수한 생육억제 효과를 나타내었다. This study was carried out to investigate antimicrobial and antitumoral activities of Candida kefyr TFP7 isolated from Tibetan fermented milk Strains of TFP1∼10 were isolated from Tibetan fermented milk by agar diffusion method using potato dextrose agar(PDA). Antimicrobial activities were examined against 18 microorganisms of food-related bacteria, yeast, algae, fungi and actinomycetes isolated from soil. Antitumor activities were examined against 9 human tumor cell lines. Strains of TFP2∼10 showed strong antimicrobial activities against Micrococcus luteus ATCC l1880, and strains of TFP6∼10 to actinomycetes, Streptomyces murinus JCM 4333. In antitumor test, all isolated strains(TEP1∼10) showed the growth inhibition of SNU-5 and SW-534 by 60% and 70%, respectively. Among those, the strain TFP7 showed the most antitumor activity, which was 77.5% for SNU-5 and 76.5% for SW-534. The strain was identified as Candida kefyr by use of API 20C AUX kit and scanning electron micrograph.
윤원호,황진용,김창한,Yoon Won-Ho,Hwang Jin-Yong,Kim Chang-Han 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.3
This study was carried out to investigate the antitumor activity from sulfur fed duck. The antitumor substances were crude purified by solvent extraction, silica gel column chromatography, and HPLC using C18 column. In MTT assay, the active compounds exhibited more cytotoxic activity on tumor cell lines than normal cell line. In addition of 100 $\mu\textrm{g}$/mL concentrations of crude purified active compounds, the growth inhibition rate of tumor cell lines was 56% (Hep-2j human larynx), 58% (KB; human epidermoid of mouth carcinoma), and 28% (MDBK; bovine normal kidney), respectively. The survival rate of clonogenic assay was 26% in Hep-2 and 28% in KB at 200 $\mu\textrm{g}$/mL. 본 연구에서는 유황오리로부터 항종양 효과를 나타내는 물질을 용매 추출법과 각종 크로마토그래피를 사용하여 분리 및 정제하였다. 유황오리로부터 정제한 활성 물질의 각종 종양세포에 대한 항암 효과를 측정한 결과 MTT assay는 100 $\mu\textrm{g}$/mL의 농도에서 HEp-2는 56%, KB는 58%의 세포 증식 억제 효과가 나타났다. 정상 세포주인 MDBK는 28%의 세포증식억제 효과가 나타나 정상 세포에 대한 세포 독성은 나타나지 않았다. Clonogenic assay는 200 $\mu\textrm{g}$/mL의 농도에서 HEp-2는 26%, KB는 28%의 생존율을 나타내었다. 따라서 유황오리로부터 정제한 활성 물질은 후두암 세포주인 HEp-2와 구강암 세포주인 KB에서만 특이적으로 세포 증식 억제 효과를 나타내었다.
윤원호,최재훈,이경호,김창한,Yoon, Won-Ho,Choi, Jae-Hoon,Lee, Keyong-Ho,Kim, Chang-Han 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1
본 연구는 폐기되는 차나무 열매를 이용하여 향균활성과 항종양활성을 비교하고 이용가능성에 대하여 탐색하고자 하였다. 항균활성과 항종양활성을 알아보기 위하여 70% 에탄올과 물 추출물의 활성을 측정하였다. 항균활성은 식중독균과 몇 종의 병원균, 효모, 곰팡이 등 17균주에 대하여 추정하였고, 항종양 활성은 6종류의 인체 암세포주, 1종류의 정상세포주에 대하여 조사하였다. 차나무 잎, 종자와 과피의 항균활성을 비교한 결과는 종자의 70% 에탄올과 물 추출물에서 효모에 대하여 항균활성이 높게 나타났다. 특히, candida albicans, Cryptococcus neoformans에 대하여 항균활성을 보였다. 종자 물 추출물을 $80,\;100^{\circ}C$에서 30분, $121^{\circ}C$에서 15분간 열처리 후 항균활성을 검색한 결과 가열온도에 따른 차이는 크게 나타나지 않았다. 항종양 활성을 검색하기 위하여 MTI assay를 통해 활성을 측정하였다. 과피 추출물에서 전혀 활성이 없었지만 종자 추출물에서는 큰 활성을 나타내었다. 극성용매인 70% 에탄올과 물 추출물의 종양세포 증식억제효과가 높게 나타났으며 그중에서 물 추출물이 70% 에탄올 추출물 보다 높게 항종양 활성이 나타났다. 차나무 종자로부터 추출한 활성물질의 종양세포와 정상 세포에 대한 세포독성을 MTT assay로 비교한 결과 $50{\mu}g/mL$의 농도에서 난소암 세포인 SK-OV-3는 70%의 억제율을 나타낸 반면 정상세포에서는 10% 미만으로 나타나 정상세포보다 종양세포에 대하여 보다 큰 선택 독성을 나타내는 것으로 보인다. Antimicrobial and antitumor activities of Camellia sinensis L seed extracts were investigated. Seed extracts showed antifungal activities against Candida albicans IFO 1594 and Cryptococcus neoformans. Inhibition zone of 20 mm was shown by 70% ethanol extract against C. albicans IFO 1594 at 100 mg/mL. Antifungal activity of seed extract was not decreased by heating at 80 and $100^{\circ}C$ for 30 min or at $121^{\circ}C$ for 15 min, indicating heat-stability of seed component. Growth-inhibitory effects were observed in 70 and 10% of tumor cell line SK-OV-3 and normal ceil line NIH/3T3 at $50{\mu}g/mL$, respectively.
尹源鎬 全北大學校 1985 論文集 Vol.27 No.-
Chinese local gazetteers dates as far back as the Dynasty of Tung-han. And it developed through the Dynasties of Sui and Tang with geographical characters. To Sung Dynasty, the humanities and history added to it, and all the social phenomena (such as geography and map, border, hillsand streams, famous places of scenic resort, officers, population, taxation, local products, villages, customs, personage, necromancy, bronze and tablet, literary pursuits, portent, etc.) and natural phenomena added to it. In the Dynasties of Yuan and Ming, it succeed the forms of local gazetteers of provious Dynasty. However to Ch'ing Dynasty, the movement which local gazetteers should be treated as a history was carried out by Chang Hsueh Cheng. Thenceforth, lots of local gazetteers were written ; so these are the tresury of historical materials. Chinese local gazetteers covers the diverse forms from the national to the local scale. Even though there are some differences in contents, it covers the above-mentioned basically and it became to have the materials of social economy as times pass. Therefore the materials of local gazetteers are concrete and individual in quality and wide enough to cover the whole nation. In this way, the values of local gazetteers are summarized as follows. First, local gazetteers covers the diverse materials of all the social phenemena and natural phenomena related to the above phenomena, so its capacity is superior to authentic records. Second, it is more valuable than authentic records chiefly because it covers the concrete facts of each local district. Third, the materials of local gazetteers are reliable chiefly because it was investigated, arranged and edited by the authorative experts. Finally, it has the contents which covers the lives of humbler classes so that it is plebeian. Besides the above-mentioned values, it is emphasized to be useful to study socio-ecomomic history because it is written more concretely than authentic records and China has the complexity of socio-economic history. In China, socio-eonomic phenomena is not seperated from humanitic phenomena. Especially under the comprehensiveness, rarity, reliability and plebeianism of materials in local gazetteers to collect and arrange the historic materials, and to describe the history are the efficient short-cut to study Chinese socio-economic history.