유색 찰옥수수의 항산화력 및 작물학적 특성

이희봉,박보영,지희정,조진웅,김석형,모은경,이미라,Lee, Hee-Bong,Park, Bo-Young,Ji, Hee-Chung,Cho, Jin-Woong,Kim, Seog-Hyung,Mo, Eun-Kyung,Lee, Mi-Ra 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

본 시험 결과를 요약하면 다음과 같다. 1. 공시교잡종의 평균 과피두께는 $37{\mu}m$로 얇은 특성을 보였는데 특히 CNU108 교잡종이 $30.3{\mu}m$로 가장 얇은 특성을 보였고 출사일수는 CNU69와 CNU202 등이 59일로 가장 빨랐으나 CNU160 교잡종은 68일로 본 교잡종들 중에서 가장 늦었다. 2. CNU70과 CNU138의 교잡종들은 높은 항산화효소(xanthin oxidase, catalase, superoxide dismutase)의 활성을 나타내었고, 특히 CNU160과 CNU193 교잡종은 각각 94.8%, 94.6% 등으로 높은 항산화력(EDA)을 나타내었다. 3. CNU109와 CNU34의 교잡종에서는 전반적으로 높은 항산화효소 활성 및 항산화력을 나타내었다. 따라서 CNU34, CNU70, CNU108, CNU138, CNU193 등의 교잡종 찰옥수수가 항산화 효과가 높은 고기능성 유색 찰옥수수인 것으로 사료되었다. This study was carried out to evaluate major agronomical characterization and phenol compound contents, Xanthine oxidase inhibitory activity (XO), Catalase activity, Superoxide dismutase activity (SOD) and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical sacvenging activity were analyzed in colored waxy corns. The mean of stem height and ear length were 248.8 cm and 18.6 cm, respectively. The pericarp thickness in CNU108 $(30.3{\mu}m)$ was thinner than other hybrids. The period of tasseling days in CNU69 and CNU202 were very shorter than other hybrids (59 days). 100-kernel weight of CNU50 was 35.6 g and heavier than the others. The antioxidant activities such as xanthin oxidase (XO), catalase and superoxide dismutase (SOD) were higher in CNU70 and CNUI 38. Especially highest EDA (electron donating ability) in DPPH radical scavenging effect was 94.8% and 94.6% in CNU160 and CNU193, respectively. In the results, the antioxidant enzyme activity and antioxidant acticity were higher in CNU109 and CNU 34 hybrids. The hybrids, CNU34, CNU70, CNU108, CNU 138 and CNU193 may be considered higher functional color waxy corn.

쥐 간 미토콘드리아 막의 알데하이드 탈수소 효소의 성질 및 기능적 재조합을 위한 조건 조사

이희봉,윤자영,주충노,Lee, Hee-Bong,Yun, Ja-Young,Joo, Chung-No 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8

본 연구의 장기적 목표로, 미토콘드리아 막에 aldehyde dehydrogenase(ALDH)가 존재하는가? 그리고 존재한다면 어떤 구조와 기능을 갖는가? 를 알기 위하여 우선 쥐 간 미토콘드리아를 sucrose의 step gradient(1~2 M) 원심분리를 이용하여 순수분리하였고, 초음파로 파쇄한 후 초원심분리를 이용하여 순수한 미토콘드리아 막 분획을 얻었다. 미토콘드리아 막 분획에 ALDH가 존재하는가를 ALDH 활성 측정으로 확인하였다. 미토콘드리아 막 ALDH를 용해시키기 위하여 여러 가지 종류(Triton X-100, digitonin, Lubrol PX, cholate 그리고 Chaps)의 detergent를 여러 가지 농도(0.5%, 0.75%, 1%, 1.5% 그리고 2%)로 사용하여 조사한 결과 1% cholate가 가장 좋은 용해 조건이었으며, 또한 용해를 위한 최적의 염(NaCl) 농도와 pH 조건은 각각 1M과 9.0이었다. 또한 용해시 5 mg/ml phospholipid 첨가가 막 ALDH 활성을 증가시키는 안정제 역할을 하였다. 5 mg/ml phospholipid와 1% cholate를 함유한 sucrose의 linear gradient(3~10%) 원심분리를 이용하여 부분정제를 한 결과 처음보다 약 100배 정제된 것을 알수 있었고, 이 부분정제된 cholate-solubilized ALDH로부터 투석을 이용하여 cholate를 제거하여 proteoliposome을 만들어 활성을 측정한 결과 그 전보다 2배 이상 증가하였다. To know the existance and structure and function of mitochondrial membrane aldehyde dehydrogenase (ALDH) as a final goal, mitochondrial membrane from rat liver was purified using sucrose step gradient (1~2 M) centrifugation, and then sonicated and ultracentrifuged to obtain pure mitochondrial membrane fraction. The observation of the ALDH activity from this fraction let us know the existance of mitochondrial membrane ALDH. The result that various kinds of detergent (Triton X-100, Digitonin, Lubrol PX, Cholate and Chaps) were examined as a good solubilizer at several different concentrations showed 1% cholate for the best one. In addition to this, the optimum salt (NaCl) concentration and pH for solubilization were 1 M and 9.0, separately. And also, the addition of phospholipid functioned as a stabilizer. The partial purification of ALDH from this cholate-solubilized membrane fraction with selected best conditions was tried using sucrose linear gradient (3~10%, including 1% cholate and 5 mg/ml phospholipid) centrifugation and was obtained more 100 times than crude mitochondrial state as a result. This partial purified, cholate-solubilized ALDH was dialyzed to remove the cholate and to make ALDH-incorporated vesicles. ALDH activity from this result was increased about 2 times than before dialysis.

쥐 간 미토콘드리아 막의 알데하이드 탈수소 효소의 성질 및 기능적 재조합을 위한 조건 조사

이희봉,윤자영,주충노 ( Hee Bong Lee,Ja Young Yun,Chung No Joo ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.8

To know the existance and structure and function of mitochondrial membrane aldehyde dehydrogenase (ALDH) as a final goal, mitochondrial membrane from rat liver was purified using sucrose step gradient (1∼2 M) centrifugation, and then sonicated and ultracentrifuged to obtain pure mitochondrial membrane fraction. The observation of the ALDH activity from this fraction let us know the existance of mitochondrial membrane ALDH. The result that various kinds of detergent (Triton X-100, Digitonin, Lubrol PX, Cholate and Chaps) were examined as a good solubilizer at several different concentrations showed 1% cholate for the best one. In addition to this, the optimum salt (NaCl) concentration and pH for solubilization were 1 M and 9.0, separately. And also, the addition of phospholipid functioned as a stabilizer. The partial purification of ALDH from this cholate-solubilized membrane fraction with selected best conditions was tried using sucrose linear gradient (3∼10%, including 1% cholate and 5 ㎎/㎖ phospholipid) centrifugation and was obtained more 100 times than crude mitochondrial state as a result. This partial purified, cholate-solubilized ALDH was dialyzed to remove the cholate and to make ALDH-incorporated vesicles. ALDH activity from this result was increased about 2 times than before dialysis.

Incorporation of Foreign Gene with Ti Plasmid Vector System: (II) Expression of E. coli Thioredoxin Gene in Cultured Tobacco Cells.

이희봉,주충노,홍순주,김성완,임창진,김영명,Lee, Hee-Bong,Joo, Chung-No,Hong, Soon-Joo,Kim, Seong-Wan,Lim, Chang-Jin,Kim, Young-Myeong 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

본 연구에서는 E. coli thioredoxin 유전자를 함유하고 있는 재조합된 plasmid pKDB3의 담배세포내로의 도입을 시도하고, 도입된 thioredoxin 유전자의 발현을 조사하였다. 담배(Nicotina tabacum cv Xanthi) 세포로의 재조합 plasmid pKDB3의 도입은 담배잎 절편과 재조합 DNA 및 helper Ti plasmid pTiBo 542를 함유하고 있는 Agrobacterium tumefaciens strain A281과의 cocultivation 방법을 이용하여 행하여졌으며, 형질전환된 담배세포는 항생물질 저항성 배지에서의 callus 형성 여부로 선별되었고, 선별된 형질전환된 calli는 shoot와 root 형성을 위해 적절한 MS agar 배지에서 계속 키워졌다. 이와 같이 형질전환된 담배세포에서 완전한 식물체로 재생된 담배잎에서의 E. coli thioredoxin 유전자의 발현을 조사한 결과 thioredoxin 활성이 형질전환된 담배세포가 정상세포에 비해 9배 정도 더 큰 것으로 나타났다. 이러한 일련의 결과들은 E. coli thioredoxin 유전자가 성공적으로 담배세포에 들어가서 높은 수준으로 발현됨을 보여주고 있다. This study was performed to observe the expression of E. coli thioredoxin gene incorporated in tobacco cells. The recombinant DNA used, pKDB3, had been constructed from a Ti plasmid vector pGA658 and a bacterial plasmid pCJF4 harboring E. coli thioredoxin gene, as described in the preceding paper (Lee et al., 1988). The leaf discs of plant (N. tabacum cv Xanthi) were transformed to kanamycin resistance by the cocultivation with Agrobacterium A281 containing plasmid pKDB3. Transformed leaf discs were cultured in MS agar medium with kanamycin for callus induction. Kanamycin-resistant tobacco calli were continuously grown in MS agar medium for shoot induction, and then in MS agar medium for root induction. Expression of E. coli thioredoxin gene in the plant tissue regenerated from transformed tobacco cells was confirmed by DTNB assay. The thioredoxin activity of transformed tobacco cells was much higher (about 9 times) than that of normal tobacco cells. Our results suggest that E. coli thioredoxin gene was successfully incorporated into tobacco cells, and the incorporated bacterial gene could be expressed at a high level.

유색미 CNU 육성계통에 대한 안토시안, 유효성분 및 주요 식물학적 특성

이희봉,김용일,박보영,정재영,송재영,최현구,강시용,김보경,Lee Hee-Bong,Kim Yong-Il,Park Bo-Young,Jung Jae-Young,Song Jae-Young,Choi Hyun-Gu,Kang Si-Yong,Kim Bo-Kyoung 한국작물학회 2005 한국작물학회지 Vol.50 No.suppl1

This study was carried out to evaluate anthocyanine contents, effective components and major botanical characteristics of the new developed colored rices. Characteristics of colored rice lines showed variation in culm length and spikelets per panicle in CNU39, panicle length in CNU43, 1,000 grain weight in CNU90, and yield per plant in CNU119 were highly compared to the Check, Heugjinjubyeo. Relative optical density(ROD) of Heugjinjubyeo was the highest at 530 nm, followed by CNU71, CNU73 and CNU119. The high peaks of ROD of Heugjinjubyeo and CNU71 appeared at 280 nm and 530 nm, and that of CNU43 appeared at 430 nm. The relative anthocyanin contents (RAC) per plant of the CU 71 among the new developed rice compared by yield per unit area was very high, fellowed by CNU73 and Heugjinjubyo. 국내${\cdot}$외에서 수집된 유색미를 분양받아 이들 에 대해 감마선처리 및 수집종간 교배육종 결과 새로 육성한 13개의 공시종에 대해 안토시안 함량, 유효성분 및 주요 수량 관련 형질을 비교 분석하였다. 1. 주요 식물학적 특성으로 대조구인 흑진주벼의 간장은 78cm로 중간형이었고, 개화일수는 다른 공시종에 비해 $7\~20$일 빨랐던 반면에 간장과 수당영화수는 CNU33에서 가장 많았으며, 수장은 CNU 43에서, 천립중은 CNU 90에서 가장 높았으며, 주당 수량은 CNU 119가 가장 높았다. 2. 공시종에 대한 Anthocyanine 색소 함량을 상대흡광도 값 (흡광도$\times$희석배수)으로 비교한 결과, 530nm파장에서 대조구인 흑진주벼가 가장 높게 나타났고, 다음은 CNU71, CNU73, CNU119의 순이었다. 흡광도 값이 가장 높았던 흑진주벼와 CNU71은 280nm와 530nm 부근에서 흡수극대가 나타나 anthocyanin계 색소임이 확인되었고, 흡광도 값이 낮았던 CNU43은 430nm부근에서 흡수극대가 나타나 tannin계 색소로 판명되었다. 3. 공시종에 대한 주당 relative anthocyanin content(RAC)을 비교하기 위해 상대흡광도 값에 주당 현미수량을 곱한 결과 CNU71이 20,112로 가장 높았고, 다음은 CNU73이 16,046 그리고 대조구인 흑진주벼 15,985순으로 나타났다.

Metabolism of Ginseng Saponin in Animal Body (I)

이희봉,주충노,Lee, Hee-Bong,Joo, Chung-No 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2

인삼(Panax ginseng C.A. Meyer) 뿌리 절편을 효소원으로 사용하여 $^{14}C$-acetate로부터 제조한 방사성 인삼 사포닌을 Thin layer chromatography, High performance liquid chromatography, Autoradiography 등으로 확인하였다. 1 mg의 인삼 사포닌을 쥐에게 구경 투여하고 1시간 후, 간장으로부터의 추출물을 분석한 결과, 회수된 방사능의 약 25%가 분해되지 않은 사포닌 상태로 존재하고 있음을 확인하였다. 또한 인삼 사포닌 투여 (구경)후 시간에 따른 방사능의 변화 양상을 초사한 결과 간에서의 사포닌의 반감기는 4~6시간, 인삼 사포닌의 농도는 $10^{-5}%$ 수준으로 예측되었다. $^{14}C$-labelled ginseng saponins from $^{14}C$-acetate were prepared using the root slices of panaxginseng C.A. Meyer as an enzyme source and the products were demonstrated to be identical with the saponins extracted from the roots by thin layer chromatography, high performace liquid chromatography and autoradiography. The radioactivity of the liver was monitored on time course after the feeding of 1 mg of ginseng saponin containing $^{14}C$-labelled saponin. The extract from the liver of rats killed at one hr. after the oral administration of 1 mg of ginseng saponins containing the $^{14}C$-saponins was analyzed found that 25% of the radioactivity recovered was found as undissociated form of the saponin. It semed that the half life of the saponin might be 4~6 hrs and the concentration of the saponin would be $10^{-5}%$ level in the liver.

Ti plasmid vector 를 이용한 진핵세포 유전자의 도입에 관한 연구

이희봉,주충노,홍순주,김성완,임창진,김영명 ( Hee Bong Lee,Chung No Joo,Sun Joo Hong,Seong Wan Kim,Chang Jin Lim,Young Myeong Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1

DNA recombination using plasmid pGA658, a Ti plasmid vector, was tried to study the role of yeast homoserine dehydrogenase after introducing its gene into plant cells. Two recombinant plasmids with opposite orientation of the gene DNA under nos promoter of vector pGA658, pKDB1 and pKDB2, were constructed by the aid of two intermediate vectors, pUC7 and pUC119. Transformed E. coli and Agrobacterium tumefaciens were confirmed by the resistance to appropriate antibiotics and by sizing DNA restriction fragments after plasmid isolation. Assay of homoserine dehydrogenase activity showed that a little increase in its activity was detected in transformed E. coli. but no increase in transformed Agrobacterium tumefaciens, compared with its untransformed strain.

Call Admission Control using Soft QoS-based Borrowing Scheme in DVB-RCS Networks

이희봉,장영민,이호진,Lee, Hee-Bong,Jang, Yeong-Min,Lee, Ho-Jin The Korean Institute of Communications and Informa 2005 韓國通信學會論文誌 Vol.30 No.2a

본 논문에서는 DVB-RCS (Digital Video Broadcast-Return Channel via Satellite) 망에서 호 수락제어 (CAC)를 위한 Soft-QoS 기반의 borrowing 기법을 제안한다. 제안된 기법은 위성 망에서 사용 가능한 자원이 부족할 때 새로운 호를 받아들이기 위해 서비스 중인 호 중 일부에서 일시적이고 공평하게 대역폭을 가져온다. 각각의 호에서 가져오는 대역폭의 양은 각 사용자의 critical bandwidth ratio에 비례하며 critical bandwidth ratio는 제안된 기법에서 이용하는 QoS 파라메타 중 하나이다. 시뮬레이션 결과는 제안된 기법이 호의 blocking 확률과 대역폭 효율 측면에서 성능의 향상이 있음을 보여준다. We propose a soft QoS-based borrowing scheme for call admission control(CAC) in DVB-RCS(Digital Video Broadcast-Return Channel via Satellite). Some of the ongoing calls temporarily and fairly release bandwidths that can be used to accommodate a new call in an overloaded satellite network. The amount of bandwidth borrowed from each call is proportional to each user's critical bandwidth ratio, one of parameters for soft QoS mechanism. Simulation results show that the proposed scheme improves the system performance in terms of call blocking probability and bandwidth utilization.

Ti Plamid Vector System 을 이용한 외래 유전자의 도입 : ( 2 ) E . coli thioredoxin 유전자의 배양된 담배세포내 발현

이희봉,주충노,홍순주,김성완,임창진,김영명 ( Hee Bong Lee,Chung No Joo,Soon Joo Hong,Seong Wan Kim,Chang Jin Lim,Young Myeong Kim ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

This study was performed to observe the expression of E. coli thioredoxin gene incorporated in tobacco cells. The recombinant DNA used, pKDB3, had been constructed from a Ti plasmid vector pGA658 and a bacterial plasmid pCJF4 harboring E. coli thioredoxin gene, as described in the preceding paper (Lee et al., 1988). The leaf discs of plant (N. tabacum cv Xanthi) were transformed to kanamycin resistance by the cocultivation with Agrobacterium A281 containing plasmid pKDB3. Transformed leaf discs were cultured in MS agar medium with kanamycin for callus induction. Kanamycin-resistant tobacco calli were continuously grown in MS agar medium for shoot induction, and then in MS agar medium for root induction. Expression of E. coli thioredoxin gene in the plant tissue regenerated from transformed tobacco cells was confirmed by DTNB assay. The thioredoxin activity of transformed tobacco cells was much higher (about 9 times) than that of normal tobacco cells. Our results suggest that E. coli thioredoxin gene was successfully incorporated into tobacco cells, and the incorporated bacterial gene could be expressed at a high level.

초음파 유도하 터널식 혈액투석용 도관 삽입술

이희봉,장근조,Lee Hee Bong,Jang Kuhn Jo 대한방사선사협회 2001 대한방사선사협회지 Vol.27 No.2

Ⅰ. Purpose : To evaluate the usefulness of ultrasound-guided, radiologic placement of tunneled hemodialysis catheters via internal jugular vein(IJV). Ⅱ. Materials and Methods : In 32 patients(31 chronic renal failure, 1 acute renal failure), We performed